Sphingobium czechense LL01

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; Sphingobium cupriresistens

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4505 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J7XS97|A0A0J7XS97_9SPHN Uncharacterized protein OS=Sphingobium czechense LL01 OX=1420583 GN=V473_14955 PE=4 SV=1
MM1 pKa = 7.79PKK3 pKa = 10.4LIVVNRR9 pKa = 11.84AGEE12 pKa = 4.04EE13 pKa = 3.9QAVDD17 pKa = 3.52GDD19 pKa = 3.97NGLSVMEE26 pKa = 5.01IIRR29 pKa = 11.84DD30 pKa = 3.56NGFDD34 pKa = 4.08EE35 pKa = 5.76LLALCGGCCSCATCHH50 pKa = 6.48VYY52 pKa = 10.53VDD54 pKa = 3.94PAFADD59 pKa = 3.63ALPAMSEE66 pKa = 4.29DD67 pKa = 4.16EE68 pKa = 4.68NDD70 pKa = 4.1LLDD73 pKa = 4.81SSDD76 pKa = 3.59HH77 pKa = 6.39RR78 pKa = 11.84NEE80 pKa = 3.85TSRR83 pKa = 11.84LSCQVMLSDD92 pKa = 4.29SLDD95 pKa = 3.54GLRR98 pKa = 11.84VTIAPEE104 pKa = 3.78DD105 pKa = 3.45

Molecular weight: 11.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J7XQ71|A0A0J7XQ71_9SPHN Multifunctional fusion protein OS=Sphingobium czechense LL01 OX=1420583 GN=cysN PE=3 SV=1
MM1 pKa = 7.49IRR3 pKa = 11.84RR4 pKa = 11.84FSPDD8 pKa = 3.14PFIVALLLAILVATMVPARR27 pKa = 11.84GPFATIVDD35 pKa = 4.15ASSTIAIVALFFLHH49 pKa = 6.76GVRR52 pKa = 11.84LPRR55 pKa = 11.84EE56 pKa = 3.86ALIQGVTHH64 pKa = 6.6WRR66 pKa = 11.84LHH68 pKa = 5.24LAILAATFVLFPLLGLGIAAAVPGLLPPPLWIGLLFLCALPSTVQSSIAFTSIAGGNVAGAVASATASNLLGIFVTPIVVGLLTQAHH155 pKa = 6.82GGAIPMSNSWKK166 pKa = 10.16IALQLLLPFILGNLCRR182 pKa = 11.84PWLGDD187 pKa = 2.9WAARR191 pKa = 11.84NKK193 pKa = 10.6KK194 pKa = 9.74IMSLCDD200 pKa = 3.04RR201 pKa = 11.84GTIIIAVYY209 pKa = 10.05SAFSAAVIAGLWQTLSWTILSSLLGICALLLAVALFVTRR248 pKa = 11.84YY249 pKa = 9.23GSRR252 pKa = 11.84LLGFSRR258 pKa = 11.84EE259 pKa = 3.99DD260 pKa = 3.37EE261 pKa = 4.14IAISFCGSKK270 pKa = 8.66KK271 pKa = 8.8TLASGVPMARR281 pKa = 11.84VLFAGPDD288 pKa = 3.26MGAVVLPLMIFHH300 pKa = 6.3QMQLIVCAALARR312 pKa = 11.84RR313 pKa = 11.84YY314 pKa = 9.98ARR316 pKa = 11.84AGRR319 pKa = 11.84QDD321 pKa = 3.5RR322 pKa = 11.84APVVASTSPSSTGG335 pKa = 3.05

Molecular weight: 35.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4505

0

4505

1355987

29

1554

301.0

32.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.352 ± 0.059

0.805 ± 0.01

6.259 ± 0.03

4.984 ± 0.036

3.499 ± 0.021

8.889 ± 0.035

2.049 ± 0.018

5.276 ± 0.022

3.141 ± 0.027

9.825 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.678 ± 0.02

2.514 ± 0.022

5.241 ± 0.027

3.301 ± 0.021

7.195 ± 0.039

5.143 ± 0.032

5.167 ± 0.026

6.983 ± 0.026

1.427 ± 0.016

2.273 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski