Eurypyga helias (Sunbittern)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia;

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7979 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A093IDJ8|A0A093IDJ8_EURHL Acyl-CoA:lysophosphatidylglycerol acyltransferase 1 OS=Eurypyga helias OX=54383 GN=N326_07511 PE=4 SV=1
LL1 pKa = 7.59FSLSLHH7 pKa = 6.68LDD9 pKa = 3.4AFAAFSAAEE18 pKa = 3.77GHH20 pKa = 6.2FFFSFFFSFFLFFFSSDD37 pKa = 3.3PSSVCTAGTLAAVVTSDD54 pKa = 4.73SLPEE58 pKa = 4.64LDD60 pKa = 3.87SSDD63 pKa = 2.81WGMEE67 pKa = 3.56RR68 pKa = 11.84SACDD72 pKa = 3.09VGVTT76 pKa = 3.97

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A093L2Y3|A0A093L2Y3_EURHL Caldesmon (Fragment) OS=Eurypyga helias OX=54383 GN=N326_11268 PE=4 SV=1
KKK2 pKa = 10.12RR3 pKa = 11.84QGKKK7 pKa = 8.85RR8 pKa = 11.84QGKKK12 pKa = 8.73RR13 pKa = 11.84QGKKK17 pKa = 8.73RR18 pKa = 11.84QGKKK22 pKa = 8.73RR23 pKa = 11.84QGKKK27 pKa = 8.73RR28 pKa = 11.84QGKKK32 pKa = 8.73RR33 pKa = 11.84QGKKK37 pKa = 8.73RR38 pKa = 11.84QGKKK42 pKa = 8.73RR43 pKa = 11.84QGKKK47 pKa = 8.55RR48 pKa = 11.84QGKKK52 pKa = 6.97EE53 pKa = 3.79KKK55 pKa = 9.54K

Molecular weight: 6.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7979

0

7979

3218414

33

4867

403.4

45.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.424 ± 0.025

2.217 ± 0.024

5.074 ± 0.02

7.164 ± 0.036

3.954 ± 0.019

5.809 ± 0.031

2.548 ± 0.013

4.967 ± 0.021

6.433 ± 0.034

9.733 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.242 ± 0.013

4.125 ± 0.017

5.283 ± 0.029

4.602 ± 0.026

5.277 ± 0.024

8.199 ± 0.038

5.379 ± 0.019

6.356 ± 0.019

1.199 ± 0.01

3.008 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski