Avian metaavulavirus 7
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C6FGZ0|C6FGZ0_9MONO Hemagglutinin-neuraminidase OS=Avian metaavulavirus 7 OX=2560317 GN=HN PE=3 SV=1
MM1 pKa = 7.92 RR2 pKa = 11.84 VRR4 pKa = 11.84 PLIIILVLLVLLWLNILPVIGLDD27 pKa = 3.34 NSKK30 pKa = 9.92 IAQAGIISAQEE41 pKa = 3.78 YY42 pKa = 9.53 AVNVYY47 pKa = 9.97 SQSNEE52 pKa = 3.37 AYY54 pKa = 8.7 IALRR58 pKa = 11.84 TVPYY62 pKa = 10.09 IPPHH66 pKa = 6.01 NLSCFQDD73 pKa = 4.53 LINTYY78 pKa = 8.44 NTTIQNIFSPIQDD91 pKa = 3.85 QITSITSASTLPSSRR106 pKa = 11.84 FAGLVVGAIALGVATSAQITAAVALTKK133 pKa = 10.39 AQQNAQEE140 pKa = 4.53 IIRR143 pKa = 11.84 LRR145 pKa = 11.84 DD146 pKa = 3.66 SIQNTINAVNDD157 pKa = 3.24 ITVGLSSIGVALSKK171 pKa = 10.05 VQNYY175 pKa = 10.03 LNDD178 pKa = 3.87 VINPALQNLSCQVSALNLGIQLNLYY203 pKa = 8.18 LTEE206 pKa = 3.8 ITTIFGPQITNPSLTPLSIQALYY229 pKa = 9.57 TLAGDD234 pKa = 4.04 NLMQFLTRR242 pKa = 11.84 YY243 pKa = 9.77 GYY245 pKa = 11.41 GEE247 pKa = 4.2 TSVSSILEE255 pKa = 3.98 SGLISAQIVSFDD267 pKa = 3.67 KK268 pKa = 10.22 QTGIAILYY276 pKa = 6.9 VTLPSIATLSGSRR289 pKa = 11.84 VTKK292 pKa = 10.54 LMSVSVQTGVGEE304 pKa = 4.46 GSAIVPSYY312 pKa = 10.7 VIQQGTVIEE321 pKa = 4.24 EE322 pKa = 4.64 FIPDD326 pKa = 3.27 SCIFTRR332 pKa = 11.84 SDD334 pKa = 3.71 VYY336 pKa = 10.36 CTQLYY341 pKa = 10.78 SKK343 pKa = 10.23 LLPDD347 pKa = 5.99 SILQCLQGSMADD359 pKa = 3.72 CQFTRR364 pKa = 11.84 SLGSFANRR372 pKa = 11.84 FMTVAGGVIANCQTVLCRR390 pKa = 11.84 CYY392 pKa = 10.24 NPVMIIPQNNGIAVTLIDD410 pKa = 4.55 GSLCKK415 pKa = 10.08 EE416 pKa = 4.31 LEE418 pKa = 4.17 LEE420 pKa = 4.73 GIRR423 pKa = 11.84 LTMADD428 pKa = 4.18 PVFASYY434 pKa = 11.11 SRR436 pKa = 11.84 DD437 pKa = 3.32 LIINGNQFAPSDD449 pKa = 3.72 ALDD452 pKa = 3.42 ISSEE456 pKa = 4.02 LGQLNNSISSATDD469 pKa = 3.24 NLQKK473 pKa = 10.82 AQEE476 pKa = 4.31 SLNKK480 pKa = 10.31 SIIPAATSSWLIILLFVLVSISLVIGCISIYY511 pKa = 10.44 FIYY514 pKa = 10.23 KK515 pKa = 9.93 HH516 pKa = 5.42 STTNRR521 pKa = 11.84 SRR523 pKa = 11.84 NLSSDD528 pKa = 3.76 IISNPYY534 pKa = 8.26 IQKK537 pKa = 10.92 ANN539 pKa = 3.44
Molecular weight: 58.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.894
IPC2_protein 5.08
IPC_protein 4.978
Toseland 4.838
ProMoST 5.105
Dawson 4.94
Bjellqvist 5.092
Wikipedia 4.825
Rodwell 4.838
Grimsley 4.749
Solomon 4.94
Lehninger 4.902
Nozaki 5.054
DTASelect 5.245
Thurlkill 4.851
EMBOSS 4.851
Sillero 5.118
Patrickios 1.163
IPC_peptide 4.952
IPC2_peptide 5.105
IPC2.peptide.svr19 5.045
Protein with the highest isoelectric point:
>tr|C6FGY9|C6FGY9_9MONO Fusion glycoprotein F0 OS=Avian metaavulavirus 7 OX=2560317 GN=F PE=3 SV=1
MM1 pKa = 7.55 EE2 pKa = 5.78 SISLGLYY9 pKa = 9.82 VDD11 pKa = 4.54 EE12 pKa = 5.46 SDD14 pKa = 4.18 PACSLLAFPIIMQTTSEE31 pKa = 4.29 GKK33 pKa = 10.02 KK34 pKa = 9.74 VLQPQVRR41 pKa = 11.84 INRR44 pKa = 11.84 LGSISIEE51 pKa = 3.93 GVRR54 pKa = 11.84 AMFINTYY61 pKa = 10.31 GFIEE65 pKa = 4.12 EE66 pKa = 4.75 RR67 pKa = 11.84 PTEE70 pKa = 3.96 RR71 pKa = 11.84 TGFFQPGEE79 pKa = 4.15 KK80 pKa = 9.96 NQQQVVTAGMLTLGQIRR97 pKa = 11.84 TNIDD101 pKa = 2.97 PDD103 pKa = 4.21 EE104 pKa = 5.55 IGEE107 pKa = 4.14 ACLRR111 pKa = 11.84 LKK113 pKa = 11.14 VNAKK117 pKa = 9.85 KK118 pKa = 10.48 SAASEE123 pKa = 4.05 EE124 pKa = 4.43 KK125 pKa = 9.97 IVFSILEE132 pKa = 4.22 KK133 pKa = 10.69 PPALMTAPVVQDD145 pKa = 3.17 GGLIAKK151 pKa = 10.21 AEE153 pKa = 4.14 GSIKK157 pKa = 10.68 CPGKK161 pKa = 9.95 MMSEE165 pKa = 3.29 IHH167 pKa = 5.82 YY168 pKa = 9.01 SFRR171 pKa = 11.84 VMFVSITMLDD181 pKa = 3.48 NQSLYY186 pKa = 10.4 RR187 pKa = 11.84 VPTAISSFKK196 pKa = 11.01 NKK198 pKa = 10.1 ALYY201 pKa = 10.27 SIQLEE206 pKa = 4.29 VLLEE210 pKa = 4.03 VDD212 pKa = 4.68 VKK214 pKa = 11.12 PEE216 pKa = 3.87 SPQCKK221 pKa = 9.41 FLADD225 pKa = 3.52 QKK227 pKa = 9.68 GKK229 pKa = 10.51 KK230 pKa = 8.37 VASVWFHH237 pKa = 6.27 LCNSKK242 pKa = 8.46 KK243 pKa = 9.2 TNASGKK249 pKa = 8.92 PRR251 pKa = 11.84 SLEE254 pKa = 3.85 DD255 pKa = 3.06 MRR257 pKa = 11.84 KK258 pKa = 9.26 KK259 pKa = 10.5 VRR261 pKa = 11.84 DD262 pKa = 3.42 MGIKK266 pKa = 10.28 VSLADD271 pKa = 3.48 LWGPTIIVRR280 pKa = 11.84 ATGKK284 pKa = 8.8 MSKK287 pKa = 10.81 YY288 pKa = 9.09 MLGFFSTSGTSCHH301 pKa = 6.37 PVTKK305 pKa = 10.45 SSPDD309 pKa = 3.17 LAKK312 pKa = 10.48 ILWSCSSTIIKK323 pKa = 10.42 ANAIVQGSVKK333 pKa = 10.16 VDD335 pKa = 3.22 VLTLEE340 pKa = 5.2 DD341 pKa = 3.68 IQVSSAAKK349 pKa = 9.59 INKK352 pKa = 9.67 SGIGKK357 pKa = 9.13 FNPFKK362 pKa = 10.81 KK363 pKa = 10.52
Molecular weight: 39.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.773
IPC2_protein 8.624
IPC_protein 8.492
Toseland 9.502
ProMoST 9.165
Dawson 9.677
Bjellqvist 9.385
Wikipedia 9.78
Rodwell 10.248
Grimsley 9.692
Solomon 9.721
Lehninger 9.692
Nozaki 9.677
DTASelect 9.311
Thurlkill 9.575
EMBOSS 9.911
Sillero 9.663
Patrickios 9.516
IPC_peptide 9.721
IPC2_peptide 7.936
IPC2.peptide.svr19 7.68
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4555
363
2227
759.2
84.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.828 ± 0.742
1.932 ± 0.36
4.83 ± 0.413
4.632 ± 0.416
3.403 ± 0.435
5.423 ± 0.383
1.361 ± 0.292
8.518 ± 0.793
5.005 ± 0.836
11.416 ± 0.725
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.261 ± 0.353
5.049 ± 0.486
4.479 ± 0.386
5.357 ± 0.619
4.193 ± 0.537
8.957 ± 0.603
6.213 ± 0.433
5.884 ± 0.393
1.076 ± 0.237
3.183 ± 0.505
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here