Calanoida sp. copepod associated circular virus
Average proteome isoelectric point is 8.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1RLR5|A0A0K1RLR5_9CIRC ATP-dependent helicase Rep OS=Calanoida sp. copepod associated circular virus OX=1692244 PE=3 SV=1
MM1 pKa = 7.14 VNSGKK6 pKa = 9.56 HH7 pKa = 3.65 WCFTLNNYY15 pKa = 7.84 TPEE18 pKa = 4.19 EE19 pKa = 4.01 EE20 pKa = 4.5 SSIKK24 pKa = 10.72 LVDD27 pKa = 3.49 AVYY30 pKa = 10.32 LVYY33 pKa = 10.62 GRR35 pKa = 11.84 EE36 pKa = 4.12 RR37 pKa = 11.84 GEE39 pKa = 3.78 SDD41 pKa = 3.35 TPHH44 pKa = 6.29 LQGFVSFDD52 pKa = 2.88 KK53 pKa = 10.81 RR54 pKa = 11.84 RR55 pKa = 11.84 TLRR58 pKa = 11.84 QVKK61 pKa = 9.43 EE62 pKa = 3.88 LLSDD66 pKa = 4.03 RR67 pKa = 11.84 VHH69 pKa = 7.01 LEE71 pKa = 3.92 SAKK74 pKa = 9.39 GTPRR78 pKa = 11.84 QASEE82 pKa = 4.16 YY83 pKa = 9.38 CKK85 pKa = 10.53 KK86 pKa = 10.83 DD87 pKa = 2.75 GDD89 pKa = 4.15 YY90 pKa = 11.21 YY91 pKa = 11.11 EE92 pKa = 5.27 VGILPGGRR100 pKa = 11.84 GTRR103 pKa = 11.84 TDD105 pKa = 3.59 LEE107 pKa = 4.56 SVRR110 pKa = 11.84 EE111 pKa = 4.17 LIKK114 pKa = 11.01 SGGSIRR120 pKa = 11.84 DD121 pKa = 3.37 VFDD124 pKa = 2.88 KK125 pKa = 11.15 HH126 pKa = 7.14 FGVALRR132 pKa = 11.84 YY133 pKa = 9.54 HH134 pKa = 7.13 RR135 pKa = 11.84 GISMAINLRR144 pKa = 11.84 GDD146 pKa = 3.05 KK147 pKa = 10.71 RR148 pKa = 11.84 EE149 pKa = 3.99 WVVDD153 pKa = 3.64 VQVYY157 pKa = 9.32 WGDD160 pKa = 3.48 TGSGKK165 pKa = 7.75 TKK167 pKa = 10.37 KK168 pKa = 10.21 AWTEE172 pKa = 3.6 APDD175 pKa = 4.27 AYY177 pKa = 10.62 VHH179 pKa = 7.35 PGGQWFDD186 pKa = 3.91 GYY188 pKa = 9.43 TGQPDD193 pKa = 4.05 VIFDD197 pKa = 4.11 DD198 pKa = 4.93 FSGSEE203 pKa = 4.02 FKK205 pKa = 10.14 LTYY208 pKa = 10.26 LLKK211 pKa = 11.04 LLDD214 pKa = 4.7 RR215 pKa = 11.84 YY216 pKa = 10.12 PMQVPVKK223 pKa = 9.76 GGFVNWVPKK232 pKa = 10.39 RR233 pKa = 11.84 IFITSNLDD241 pKa = 2.82 PRR243 pKa = 11.84 TWYY246 pKa = 10.35 KK247 pKa = 10.97 DD248 pKa = 3.34 AIQEE252 pKa = 4.02 HH253 pKa = 5.49 QAALMRR259 pKa = 11.84 RR260 pKa = 11.84 ITKK263 pKa = 8.43 ITHH266 pKa = 4.58 FTIPFHH272 pKa = 6.72
Molecular weight: 31.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.148
IPC2_protein 8.185
IPC_protein 8.185
Toseland 8.653
ProMoST 8.565
Dawson 8.975
Bjellqvist 8.858
Wikipedia 9.136
Rodwell 9.092
Grimsley 8.96
Solomon 9.121
Lehninger 9.077
Nozaki 8.887
DTASelect 8.785
Thurlkill 8.873
EMBOSS 9.121
Sillero 9.033
Patrickios 4.558
IPC_peptide 9.107
IPC2_peptide 7.585
IPC2.peptide.svr19 7.595
Protein with the highest isoelectric point:
>tr|A0A0K1RLR5|A0A0K1RLR5_9CIRC ATP-dependent helicase Rep OS=Calanoida sp. copepod associated circular virus OX=1692244 PE=3 SV=1
MM1 pKa = 7.03 VSSMTPYY8 pKa = 10.19 SRR10 pKa = 11.84 SRR12 pKa = 11.84 YY13 pKa = 8.7 GGVWLPAARR22 pKa = 11.84 AAGRR26 pKa = 11.84 LAIRR30 pKa = 11.84 AGSRR34 pKa = 11.84 YY35 pKa = 7.9 VARR38 pKa = 11.84 LLGKK42 pKa = 9.95 RR43 pKa = 11.84 KK44 pKa = 9.65 RR45 pKa = 11.84 ATTTAHH51 pKa = 5.47 QPRR54 pKa = 11.84 KK55 pKa = 9.52 RR56 pKa = 11.84 VYY58 pKa = 8.85 TKK60 pKa = 9.1 TKK62 pKa = 9.69 RR63 pKa = 11.84 YY64 pKa = 6.09 MTGGYY69 pKa = 8.79 IGRR72 pKa = 11.84 RR73 pKa = 11.84 VKK75 pKa = 10.13 RR76 pKa = 11.84 AKK78 pKa = 10.24 RR79 pKa = 11.84 RR80 pKa = 11.84 TRR82 pKa = 11.84 QNAATLYY89 pKa = 8.97 GCSLKK94 pKa = 10.01 TEE96 pKa = 4.43 LGGVVTDD103 pKa = 4.05 SKK105 pKa = 11.4 AVYY108 pKa = 9.76 VGHH111 pKa = 7.16 CSVQPEE117 pKa = 5.26 LILKK121 pKa = 9.65 AAFRR125 pKa = 11.84 AIVKK129 pKa = 10.01 KK130 pKa = 10.68 LYY132 pKa = 10.26 DD133 pKa = 3.51 GAGIRR138 pKa = 11.84 IASWMDD144 pKa = 3.19 KK145 pKa = 10.01 PVNPAVLYY153 pKa = 10.5 CKK155 pKa = 10.32 LYY157 pKa = 10.15 NQPASQSLNTLSYY170 pKa = 10.44 TLLATDD176 pKa = 4.57 TYY178 pKa = 11.35 QDD180 pKa = 3.24 IAEE183 pKa = 4.4 GLLNVVVNYY192 pKa = 10.36 INLTVAINTLPTFTYY207 pKa = 10.5 FSLQHH212 pKa = 6.41 GGTAVAEE219 pKa = 4.22 IQAPQARR226 pKa = 11.84 FYY228 pKa = 11.3 VSTYY232 pKa = 9.62 STLTVQNRR240 pKa = 11.84 TLAGVSADD248 pKa = 3.44 NDD250 pKa = 3.19 EE251 pKa = 4.43 STNVSKK257 pKa = 11.38 NPVHH261 pKa = 6.55 GKK263 pKa = 9.74 LYY265 pKa = 10.23 CGRR268 pKa = 11.84 GNFFEE273 pKa = 4.49 PSYY276 pKa = 10.79 RR277 pKa = 11.84 DD278 pKa = 3.45 PNDD281 pKa = 3.38 ISWVPLVCGRR291 pKa = 11.84 TFGSMAATASGSLPLDD307 pKa = 3.35 GMKK310 pKa = 10.35 PPEE313 pKa = 3.92 GSYY316 pKa = 10.89 FSNCMSTSRR325 pKa = 11.84 ITIKK329 pKa = 10.19 PGEE332 pKa = 4.09 IRR334 pKa = 11.84 RR335 pKa = 11.84 SVLKK339 pKa = 10.05 YY340 pKa = 9.82 KK341 pKa = 10.59 RR342 pKa = 11.84 SFQFTEE348 pKa = 4.35 LFHH351 pKa = 8.06 QLFNLIKK358 pKa = 10.09 VYY360 pKa = 10.62 SLSAQVPTMLGNMNMFGLEE379 pKa = 3.9 KK380 pKa = 10.71 LLDD383 pKa = 3.92 SRR385 pKa = 11.84 SDD387 pKa = 3.5 EE388 pKa = 4.14 PDD390 pKa = 2.84 ISIGWEE396 pKa = 3.82 VNNTVDD402 pKa = 4.23 CGLSIKK408 pKa = 10.27 RR409 pKa = 11.84 CNPPAEE415 pKa = 4.42 VIEE418 pKa = 4.41 TT419 pKa = 3.75
Molecular weight: 46.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.837
IPC2_protein 9.385
IPC_protein 9.458
Toseland 9.765
ProMoST 9.619
Dawson 10.072
Bjellqvist 9.823
Wikipedia 10.277
Rodwell 10.306
Grimsley 10.16
Solomon 10.087
Lehninger 10.043
Nozaki 9.853
DTASelect 9.794
Thurlkill 9.897
EMBOSS 10.204
Sillero 9.999
Patrickios 4.991
IPC_peptide 10.072
IPC2_peptide 8.712
IPC2.peptide.svr19 8.261
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
691
272
419
345.5
38.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.512 ± 1.469
1.447 ± 0.424
4.92 ± 1.448
4.197 ± 0.784
3.763 ± 0.605
8.104 ± 0.647
2.026 ± 0.764
4.631 ± 0.088
6.078 ± 0.54
8.249 ± 0.533
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.026 ± 0.331
3.907 ± 1.013
4.776 ± 0.217
3.184 ± 0.074
7.381 ± 0.016
7.381 ± 0.892
7.236 ± 0.587
7.525 ± 0.116
1.592 ± 0.584
5.065 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here