Tibetan frog hepatitis B virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Blubervirales; Hepadnaviridae; Herpetohepadnavirus

Average proteome isoelectric point is 8.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A193AUB7|A0A193AUB7_9HEPA Isoform of A0A193AU90 Large envelope protein OS=Tibetan frog hepatitis B virus OX=2169919 GN=PreS PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84FFARR6 pKa = 11.84VWSWIGGNPSQHH18 pKa = 5.7VSAQEE23 pKa = 3.64HH24 pKa = 6.68RR25 pKa = 11.84DD26 pKa = 3.61RR27 pKa = 11.84MEE29 pKa = 4.8DD30 pKa = 3.05PHH32 pKa = 8.71AFQQYY37 pKa = 10.19LATLPDD43 pKa = 3.47INRR46 pKa = 11.84AALLSLNKK54 pKa = 9.91HH55 pKa = 5.56FRR57 pKa = 11.84LDD59 pKa = 3.37NHH61 pKa = 6.58QGFLQILDD69 pKa = 3.51NATFAEE75 pKa = 4.48IAEE78 pKa = 4.17HH79 pKa = 6.38YY80 pKa = 10.35YY81 pKa = 10.69RR82 pKa = 11.84FRR84 pKa = 11.84QAYY87 pKa = 8.85ANHH90 pKa = 6.94SITWTPPHH98 pKa = 6.42EE99 pKa = 4.03EE100 pKa = 4.5EE101 pKa = 4.36YY102 pKa = 10.18WEE104 pKa = 4.02EE105 pKa = 3.68HH106 pKa = 5.64ALYY109 pKa = 9.41PEE111 pKa = 4.21KK112 pKa = 10.45AAPVEE117 pKa = 4.22PIIPVPGPKK126 pKa = 9.33RR127 pKa = 11.84APPLKK132 pKa = 10.07PRR134 pKa = 11.84PQPRR138 pKa = 11.84NRR140 pKa = 11.84TKK142 pKa = 10.65LRR144 pKa = 11.84KK145 pKa = 9.1PSDD148 pKa = 4.59AIPVLLAIPTSSSTEE163 pKa = 3.99VGRR166 pKa = 11.84LPYY169 pKa = 10.08QIYY172 pKa = 9.16PHH174 pKa = 6.95EE175 pKa = 4.85LLPIPKK181 pKa = 9.7LEE183 pKa = 4.39EE184 pKa = 4.07KK185 pKa = 9.78STTANMSGAVSPALWFPVVALVVYY209 pKa = 9.41FLLTKK214 pKa = 10.47ARR216 pKa = 11.84EE217 pKa = 3.99ILAKK221 pKa = 10.3LDD223 pKa = 3.46WWWTSHH229 pKa = 5.78SFRR232 pKa = 11.84GANGEE237 pKa = 5.09CIFQDD242 pKa = 4.41TSPQTQKK249 pKa = 10.86HH250 pKa = 5.13LAGFCPLTCGGFLWTSLRR268 pKa = 11.84LFTICLIILLAVCGYY283 pKa = 10.13LFLMEE288 pKa = 5.5KK289 pKa = 10.7GFTSLGEE296 pKa = 4.08QLWEE300 pKa = 4.46LGSAPGTLLSSLLSSLKK317 pKa = 10.27GSGNIFLSIALLTWMTSSYY336 pKa = 10.02LTLTPATSLLLLTGLWATYY355 pKa = 11.26NNMGSGG361 pKa = 3.47

Molecular weight: 40.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A193AU90|A0A193AU90_9HEPA Large envelope protein OS=Tibetan frog hepatitis B virus OX=2169919 GN=PreS PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 10.31RR3 pKa = 11.84LWSRR7 pKa = 11.84NVRR10 pKa = 11.84GRR12 pKa = 11.84PGPILEE18 pKa = 4.34EE19 pKa = 4.21LEE21 pKa = 4.46GALTQAGTEE30 pKa = 4.13DD31 pKa = 4.15YY32 pKa = 10.4IIRR35 pKa = 11.84GQSGDD40 pKa = 4.54LIVQGKK46 pKa = 9.34EE47 pKa = 3.87DD48 pKa = 4.41LDD50 pKa = 4.55GGVLPLQAEE59 pKa = 4.63PEE61 pKa = 4.18RR62 pKa = 11.84LLGPLKK68 pKa = 10.63GLYY71 pKa = 10.17QNDD74 pKa = 3.68VPPFNPSWEE83 pKa = 4.1IPTIQGVHH91 pKa = 5.28LQEE94 pKa = 5.24DD95 pKa = 4.99IIRR98 pKa = 11.84EE99 pKa = 3.96IPDD102 pKa = 3.24RR103 pKa = 11.84RR104 pKa = 11.84WAYY107 pKa = 9.67IYY109 pKa = 9.53PIRR112 pKa = 11.84FWPNEE117 pKa = 3.6VKK119 pKa = 10.52YY120 pKa = 10.4RR121 pKa = 11.84PRR123 pKa = 11.84EE124 pKa = 3.6QGITDD129 pKa = 4.41KK130 pKa = 11.33YY131 pKa = 9.37PEE133 pKa = 3.98HH134 pKa = 6.21TRR136 pKa = 11.84AHH138 pKa = 5.52SEE140 pKa = 4.08KK141 pKa = 10.57VSLYY145 pKa = 10.91LQTLYY150 pKa = 11.51NNGILYY156 pKa = 10.03KK157 pKa = 10.21RR158 pKa = 11.84EE159 pKa = 3.99SRR161 pKa = 11.84HH162 pKa = 5.58VLLFNDD168 pKa = 4.58NPYY171 pKa = 10.65SWEE174 pKa = 4.28SNNLLPCHH182 pKa = 5.69CHH184 pKa = 6.97KK185 pKa = 11.11SQDD188 pKa = 3.74YY189 pKa = 10.29EE190 pKa = 4.29GLGRR194 pKa = 11.84QPPSRR199 pKa = 11.84RR200 pKa = 11.84SNEE203 pKa = 3.58VLRR206 pKa = 11.84PSVEE210 pKa = 3.88LDD212 pKa = 2.73RR213 pKa = 11.84GKK215 pKa = 9.97PLTTRR220 pKa = 11.84LSPRR224 pKa = 11.84AQRR227 pKa = 11.84PDD229 pKa = 3.01GRR231 pKa = 11.84PSRR234 pKa = 11.84FPAILSNPAGHH245 pKa = 5.97QPSGIVKK252 pKa = 9.52FKK254 pKa = 9.42QTLPAGQSPRR264 pKa = 11.84ISTDD268 pKa = 2.34TRR270 pKa = 11.84QCHH273 pKa = 4.33VRR275 pKa = 11.84GNSRR279 pKa = 11.84TLLQIPPSICQPQHH293 pKa = 6.93NMDD296 pKa = 3.52SSPRR300 pKa = 11.84GGILGGTRR308 pKa = 11.84PLSRR312 pKa = 11.84EE313 pKa = 4.0GGSGRR318 pKa = 11.84TYY320 pKa = 9.53HH321 pKa = 6.15TGSRR325 pKa = 11.84AQKK328 pKa = 9.91SSSSKK333 pKa = 9.64ATSSTPKK340 pKa = 9.6QDD342 pKa = 3.38KK343 pKa = 9.65AQEE346 pKa = 4.19AIRR349 pKa = 11.84RR350 pKa = 11.84HH351 pKa = 4.41TCAARR356 pKa = 11.84NSYY359 pKa = 9.12QQLNRR364 pKa = 11.84SRR366 pKa = 11.84PASISNLPPRR376 pKa = 11.84VTTYY380 pKa = 10.67PEE382 pKa = 4.15AGRR385 pKa = 11.84KK386 pKa = 8.63EE387 pKa = 4.23YY388 pKa = 10.55NCKK391 pKa = 10.1HH392 pKa = 5.59EE393 pKa = 4.28RR394 pKa = 11.84RR395 pKa = 11.84CFSRR399 pKa = 11.84SVVPSGCTGRR409 pKa = 11.84VFLVNKK415 pKa = 10.37SSGNSSQARR424 pKa = 11.84LVVDD428 pKa = 4.9FSQFSRR434 pKa = 11.84GKK436 pKa = 10.11RR437 pKa = 11.84RR438 pKa = 11.84VHH440 pKa = 6.11FPRR443 pKa = 11.84YY444 pKa = 9.23FSPNTEE450 pKa = 3.89ALSRR454 pKa = 11.84ILPPHH459 pKa = 6.05MWRR462 pKa = 11.84ISLDD466 pKa = 3.43LAEE469 pKa = 5.16AFYY472 pKa = 10.67HH473 pKa = 6.54LPYY476 pKa = 9.06HH477 pKa = 6.3TSSSLRR483 pKa = 11.84LSVSDD488 pKa = 3.54GKK490 pKa = 10.46RR491 pKa = 11.84VYY493 pKa = 10.7LFRR496 pKa = 11.84RR497 pKa = 11.84AAMGVGLSPWNLTLFTSIIAEE518 pKa = 4.52RR519 pKa = 11.84IRR521 pKa = 11.84KK522 pKa = 8.41YY523 pKa = 10.97FSIHH527 pKa = 5.81CFAYY531 pKa = 9.43MDD533 pKa = 4.75DD534 pKa = 5.59FILSHH539 pKa = 6.66PNPRR543 pKa = 11.84YY544 pKa = 10.28LIAAANGVVGYY555 pKa = 8.56LQQYY559 pKa = 7.3GVRR562 pKa = 11.84INFDD566 pKa = 3.45KK567 pKa = 10.8STLTPTRR574 pKa = 11.84QIVFMGLQITPTHH587 pKa = 5.01IHH589 pKa = 4.06VTKK592 pKa = 10.68EE593 pKa = 3.88KK594 pKa = 10.15FQKK597 pKa = 10.65LHH599 pKa = 7.25DD600 pKa = 4.91LLTSINTDD608 pKa = 2.48VFYY611 pKa = 10.73DD612 pKa = 3.41WKK614 pKa = 10.37ILQRR618 pKa = 11.84FAGHH622 pKa = 6.6FNQIVAFSLFGSHH635 pKa = 6.63ILGPIYY641 pKa = 10.48LAITNKK647 pKa = 10.35ADD649 pKa = 3.34FCFTKK654 pKa = 10.41SYY656 pKa = 9.54VTDD659 pKa = 3.56LRR661 pKa = 11.84YY662 pKa = 10.34VLNHH666 pKa = 6.19VYY668 pKa = 10.71DD669 pKa = 3.84LRR671 pKa = 11.84LRR673 pKa = 11.84ASPKK677 pKa = 10.22HH678 pKa = 5.6IVPSVAADD686 pKa = 3.24ATLTHH691 pKa = 6.63GGIATRR697 pKa = 11.84QGEE700 pKa = 4.57EE701 pKa = 3.59IQLTFSEE708 pKa = 4.24QRR710 pKa = 11.84PVHH713 pKa = 4.89VQEE716 pKa = 4.8LLIALVALLCFVPKK730 pKa = 10.69GLLSDD735 pKa = 3.72STFVVHH741 pKa = 6.6KK742 pKa = 10.13QLHH745 pKa = 4.94TLPYY749 pKa = 8.59ALAAVGHH756 pKa = 5.83YY757 pKa = 10.1LLSRR761 pKa = 11.84AKK763 pKa = 8.45VTYY766 pKa = 10.41VHH768 pKa = 6.77TSLNPADD775 pKa = 3.8PVSRR779 pKa = 11.84LLANHH784 pKa = 6.53SAPRR788 pKa = 11.84WPLLVHH794 pKa = 6.22TSLVKK799 pKa = 10.04PLYY802 pKa = 10.17IPWSLLRR809 pKa = 11.84SPP811 pKa = 4.9

Molecular weight: 91.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

1

4

1928

266

811

482.0

54.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.587 ± 0.679

1.297 ± 0.215

3.06 ± 0.472

4.824 ± 0.496

4.253 ± 0.301

6.172 ± 0.302

3.683 ± 0.262

5.394 ± 0.293

3.631 ± 0.578

11.463 ± 0.736

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.297 ± 0.317

3.786 ± 0.256

7.988 ± 0.253

4.253 ± 0.371

7.573 ± 1.401

8.039 ± 0.626

6.38 ± 0.618

4.409 ± 0.833

2.127 ± 0.442

3.786 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski