Proteome-pI 2.0 - Proteome Isoelectric Point Database

Ophiostoma mitovirus 5

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Howeltoviricetes; Cryppavirales; Mitoviridae; Mitovirus

Average proteome isoelectric point is 8.81

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|Q9WJ23|Q9WJ23_9VIRU RNA-dependent RNA polymerase  putative OS=Ophiostoma mitovirus 5 OX=88388 GN=pol5 PE=2 SV=1

MM1 pKa = 7.43KK2 pKa = 10.29KK3 pKa = 9.69INKK6 pKa = 7.68TIKK9 pKa = 9.69ILLSIYY15 pKa = 9.99FNRR18 pKa = 11.84KK19 pKa = 6.87YY20 pKa = 10.7SSYY23 pKa = 9.4GIRR26 pKa = 11.84WIVTVEE32 pKa = 3.87RR33 pKa = 11.84MRR35 pKa = 11.84KK36 pKa = 8.97INGLKK41 pKa = 9.17FTIKK45 pKa = 10.38YY46 pKa = 7.18MKK48 pKa = 10.09AVKK51 pKa = 10.15LHH53 pKa = 4.49ITKK56 pKa = 10.71YY57 pKa = 9.38IANEE61 pKa = 3.84RR62 pKa = 11.84LLSISGSRR70 pKa = 11.84VSVDD74 pKa = 2.81KK75 pKa = 11.45DD76 pKa = 3.38GFPTKK81 pKa = 10.55FNYY84 pKa = 9.34IKK86 pKa = 10.52HH87 pKa = 6.56IIDD90 pKa = 3.81SGDD93 pKa = 2.9IDD95 pKa = 4.41GIRR98 pKa = 11.84FVMTLLTYY106 pKa = 9.83TRR108 pKa = 11.84AINPTKK114 pKa = 10.47KK115 pKa = 9.88EE116 pKa = 3.92YY117 pKa = 11.1LKK119 pKa = 10.6IFPDD123 pKa = 3.66YY124 pKa = 10.78STITNEE130 pKa = 3.84FTGSNKK136 pKa = 9.4IAIPNKK142 pKa = 9.42IIKK145 pKa = 10.04EE146 pKa = 3.9FVDD149 pKa = 4.21YY150 pKa = 11.4YY151 pKa = 11.67KK152 pKa = 10.93LANPNNNNNPEE163 pKa = 4.29LQWSRR168 pKa = 11.84DD169 pKa = 3.28DD170 pKa = 4.4HH171 pKa = 6.22YY172 pKa = 11.86LSFKK176 pKa = 10.68SSPNGQSTLHH186 pKa = 5.89SSYY189 pKa = 11.46GLFSMIFVGHH199 pKa = 6.94TILEE203 pKa = 4.61NILKK207 pKa = 10.09IVGEE211 pKa = 4.18KK212 pKa = 10.02QYY214 pKa = 11.36HH215 pKa = 5.64EE216 pKa = 4.89IIGNHH221 pKa = 5.2IKK223 pKa = 10.55KK224 pKa = 10.05LYY226 pKa = 9.05HH227 pKa = 5.79DD228 pKa = 4.53HH229 pKa = 7.39RR230 pKa = 11.84LFIPGKK236 pKa = 9.34IDD238 pKa = 3.35YY239 pKa = 10.17LFGKK243 pKa = 10.22ISIVKK248 pKa = 10.25DD249 pKa = 3.47PEE251 pKa = 3.85LKK253 pKa = 9.62MRR255 pKa = 11.84VIAMVDD261 pKa = 3.13YY262 pKa = 10.85HH263 pKa = 6.46SQFVLKK269 pKa = 10.23KK270 pKa = 9.26IHH272 pKa = 6.51NSLFNKK278 pKa = 10.21LKK280 pKa = 10.49LIKK283 pKa = 9.88SDD285 pKa = 3.36RR286 pKa = 11.84TFTQDD291 pKa = 4.7PIFTTPTMGHH301 pKa = 6.65RR302 pKa = 11.84FWSMDD307 pKa = 3.09LSAATDD313 pKa = 3.88RR314 pKa = 11.84FPIDD318 pKa = 3.36LQEE321 pKa = 4.39RR322 pKa = 11.84LLSYY326 pKa = 10.72LYY328 pKa = 10.81GSEE331 pKa = 4.86ISSAWKK337 pKa = 9.83QLLIDD342 pKa = 3.94RR343 pKa = 11.84TYY345 pKa = 9.61KK346 pKa = 10.1TPEE349 pKa = 4.03GDD351 pKa = 3.36EE352 pKa = 3.87LHH354 pKa = 6.76YY355 pKa = 11.01KK356 pKa = 9.84VGQPMGAYY364 pKa = 9.89SSWAAFTLTHH374 pKa = 6.39HH375 pKa = 6.76LVVFYY380 pKa = 9.34SARR383 pKa = 11.84MAGIKK388 pKa = 10.64DD389 pKa = 3.32FTNYY393 pKa = 9.8ILLGDD398 pKa = 5.23DD399 pKa = 3.71IVINNDD405 pKa = 2.84KK406 pKa = 9.53VAKK409 pKa = 10.21YY410 pKa = 9.66YY411 pKa = 10.2IRR413 pKa = 11.84TMKK416 pKa = 10.64RR417 pKa = 11.84LGVEE421 pKa = 3.83LSMNKK426 pKa = 7.79THH428 pKa = 6.34VSKK431 pKa = 9.8NTYY434 pKa = 9.61EE435 pKa = 4.03FAKK438 pKa = 10.3RR439 pKa = 11.84WFKK442 pKa = 10.86NKK444 pKa = 10.17KK445 pKa = 9.43EE446 pKa = 4.04ITGLPLRR453 pKa = 11.84GILNNLNNYY462 pKa = 9.71GIVFQEE468 pKa = 4.09LFKK471 pKa = 10.81FHH473 pKa = 6.45YY474 pKa = 9.82KK475 pKa = 10.25YY476 pKa = 10.6PHH478 pKa = 5.88LTNVKK483 pKa = 8.61LTDD486 pKa = 2.87IMFIIFKK493 pKa = 9.83GLKK496 pKa = 8.39IKK498 pKa = 10.6GRR500 pKa = 11.84IITNSQLRR508 pKa = 11.84FNLMKK513 pKa = 10.44INFLLRR519 pKa = 11.84YY520 pKa = 8.94INKK523 pKa = 9.4LVNFDD528 pKa = 3.76EE529 pKa = 4.35TRR531 pKa = 11.84LFYY534 pKa = 10.74TKK536 pKa = 10.49FIKK539 pKa = 10.65SEE541 pKa = 4.57DD542 pKa = 3.06ISMVNEE548 pKa = 3.89HH549 pKa = 6.53NFLDD553 pKa = 4.61FTRR556 pKa = 11.84GMLKK560 pKa = 10.56LGLTQKK566 pKa = 10.16IEE568 pKa = 4.06NSVKK572 pKa = 10.03EE573 pKa = 4.36LKK575 pKa = 9.58TFYY578 pKa = 11.19DD579 pKa = 3.95DD580 pKa = 3.38VLKK583 pKa = 11.22NSFISNIEE591 pKa = 3.97NKK593 pKa = 9.98NDD595 pKa = 3.46LQYY598 pKa = 11.38EE599 pKa = 4.0PLINGLYY606 pKa = 10.59NKK608 pKa = 9.03MLIMRR613 pKa = 11.84NSIDD617 pKa = 3.56RR618 pKa = 11.84IVRR621 pKa = 11.84NKK623 pKa = 10.81DD624 pKa = 2.81FDD626 pKa = 5.74IIDD629 pKa = 3.78AMNDD633 pKa = 3.02MRR635 pKa = 11.84LDD637 pKa = 3.55NPEE640 pKa = 4.39AYY642 pKa = 10.25LEE644 pKa = 4.45KK645 pKa = 10.32IKK647 pKa = 11.09NSNKK651 pKa = 9.66PLSNLNDD658 pKa = 3.52MFNTAKK664 pKa = 10.45KK665 pKa = 9.98RR666 pKa = 11.84IKK668 pKa = 10.04EE669 pKa = 3.81INEE672 pKa = 3.75YY673 pKa = 10.18NSEE676 pKa = 4.21YY677 pKa = 10.74FQDD680 pKa = 5.08IYY682 pKa = 11.82DD683 pKa = 4.16FDD685 pKa = 3.91NFSNFRR691 pKa = 11.84PYY693 pKa = 10.48EE694 pKa = 3.91SYY696 pKa = 11.24YY697 pKa = 10.43RR698 pKa = 11.84AEE700 pKa = 4.97LSTQIDD706 pKa = 4.15NLDD709 pKa = 4.24MIRR712 pKa = 11.84GAYY715 pKa = 7.86WRR717 pKa = 11.84DD718 pKa = 3.4PQKK721 pKa = 9.81EE722 pKa = 4.44TEE724 pKa = 4.08MLQYY728 pKa = 9.37WW729 pKa = 3.71

Molecular weight: 86.19 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|Q9WJ23|Q9WJ23_9VIRU RNA-dependent RNA polymerase  putative OS=Ophiostoma mitovirus 5 OX=88388 GN=pol5 PE=2 SV=1

MM1 pKa = 7.43KK2 pKa = 10.29KK3 pKa = 9.69INKK6 pKa = 7.68TIKK9 pKa = 9.69ILLSIYY15 pKa = 9.99FNRR18 pKa = 11.84KK19 pKa = 6.87YY20 pKa = 10.7SSYY23 pKa = 9.4GIRR26 pKa = 11.84WIVTVEE32 pKa = 3.87RR33 pKa = 11.84MRR35 pKa = 11.84KK36 pKa = 8.97INGLKK41 pKa = 9.17FTIKK45 pKa = 10.38YY46 pKa = 7.18MKK48 pKa = 10.09AVKK51 pKa = 10.15LHH53 pKa = 4.49ITKK56 pKa = 10.71YY57 pKa = 9.38IANEE61 pKa = 3.84RR62 pKa = 11.84LLSISGSRR70 pKa = 11.84VSVDD74 pKa = 2.81KK75 pKa = 11.45DD76 pKa = 3.38GFPTKK81 pKa = 10.55FNYY84 pKa = 9.34IKK86 pKa = 10.52HH87 pKa = 6.56IIDD90 pKa = 3.81SGDD93 pKa = 2.9IDD95 pKa = 4.41GIRR98 pKa = 11.84FVMTLLTYY106 pKa = 9.83TRR108 pKa = 11.84AINPTKK114 pKa = 10.47KK115 pKa = 9.88EE116 pKa = 3.92YY117 pKa = 11.1LKK119 pKa = 10.6IFPDD123 pKa = 3.66YY124 pKa = 10.78STITNEE130 pKa = 3.84FTGSNKK136 pKa = 9.4IAIPNKK142 pKa = 9.42IIKK145 pKa = 10.04EE146 pKa = 3.9FVDD149 pKa = 4.21YY150 pKa = 11.4YY151 pKa = 11.67KK152 pKa = 10.93LANPNNNNNPEE163 pKa = 4.29LQWSRR168 pKa = 11.84DD169 pKa = 3.28DD170 pKa = 4.4HH171 pKa = 6.22YY172 pKa = 11.86LSFKK176 pKa = 10.68SSPNGQSTLHH186 pKa = 5.89SSYY189 pKa = 11.46GLFSMIFVGHH199 pKa = 6.94TILEE203 pKa = 4.61NILKK207 pKa = 10.09IVGEE211 pKa = 4.18KK212 pKa = 10.02QYY214 pKa = 11.36HH215 pKa = 5.64EE216 pKa = 4.89IIGNHH221 pKa = 5.2IKK223 pKa = 10.55KK224 pKa = 10.05LYY226 pKa = 9.05HH227 pKa = 5.79DD228 pKa = 4.53HH229 pKa = 7.39RR230 pKa = 11.84LFIPGKK236 pKa = 9.34IDD238 pKa = 3.35YY239 pKa = 10.17LFGKK243 pKa = 10.22ISIVKK248 pKa = 10.25DD249 pKa = 3.47PEE251 pKa = 3.85LKK253 pKa = 9.62MRR255 pKa = 11.84VIAMVDD261 pKa = 3.13YY262 pKa = 10.85HH263 pKa = 6.46SQFVLKK269 pKa = 10.23KK270 pKa = 9.26IHH272 pKa = 6.51NSLFNKK278 pKa = 10.21LKK280 pKa = 10.49LIKK283 pKa = 9.88SDD285 pKa = 3.36RR286 pKa = 11.84TFTQDD291 pKa = 4.7PIFTTPTMGHH301 pKa = 6.65RR302 pKa = 11.84FWSMDD307 pKa = 3.09LSAATDD313 pKa = 3.88RR314 pKa = 11.84FPIDD318 pKa = 3.36LQEE321 pKa = 4.39RR322 pKa = 11.84LLSYY326 pKa = 10.72LYY328 pKa = 10.81GSEE331 pKa = 4.86ISSAWKK337 pKa = 9.83QLLIDD342 pKa = 3.94RR343 pKa = 11.84TYY345 pKa = 9.61KK346 pKa = 10.1TPEE349 pKa = 4.03GDD351 pKa = 3.36EE352 pKa = 3.87LHH354 pKa = 6.76YY355 pKa = 11.01KK356 pKa = 9.84VGQPMGAYY364 pKa = 9.89SSWAAFTLTHH374 pKa = 6.39HH375 pKa = 6.76LVVFYY380 pKa = 9.34SARR383 pKa = 11.84MAGIKK388 pKa = 10.64DD389 pKa = 3.32FTNYY393 pKa = 9.8ILLGDD398 pKa = 5.23DD399 pKa = 3.71IVINNDD405 pKa = 2.84KK406 pKa = 9.53VAKK409 pKa = 10.21YY410 pKa = 9.66YY411 pKa = 10.2IRR413 pKa = 11.84TMKK416 pKa = 10.64RR417 pKa = 11.84LGVEE421 pKa = 3.83LSMNKK426 pKa = 7.79THH428 pKa = 6.34VSKK431 pKa = 9.8NTYY434 pKa = 9.61EE435 pKa = 4.03FAKK438 pKa = 10.3RR439 pKa = 11.84WFKK442 pKa = 10.86NKK444 pKa = 10.17KK445 pKa = 9.43EE446 pKa = 4.04ITGLPLRR453 pKa = 11.84GILNNLNNYY462 pKa = 9.71GIVFQEE468 pKa = 4.09LFKK471 pKa = 10.81FHH473 pKa = 6.45YY474 pKa = 9.82KK475 pKa = 10.25YY476 pKa = 10.6PHH478 pKa = 5.88LTNVKK483 pKa = 8.61LTDD486 pKa = 2.87IMFIIFKK493 pKa = 9.83GLKK496 pKa = 8.39IKK498 pKa = 10.6GRR500 pKa = 11.84IITNSQLRR508 pKa = 11.84FNLMKK513 pKa = 10.44INFLLRR519 pKa = 11.84YY520 pKa = 8.94INKK523 pKa = 9.4LVNFDD528 pKa = 3.76EE529 pKa = 4.35TRR531 pKa = 11.84LFYY534 pKa = 10.74TKK536 pKa = 10.49FIKK539 pKa = 10.65SEE541 pKa = 4.57DD542 pKa = 3.06ISMVNEE548 pKa = 3.89HH549 pKa = 6.53NFLDD553 pKa = 4.61FTRR556 pKa = 11.84GMLKK560 pKa = 10.56LGLTQKK566 pKa = 10.16IEE568 pKa = 4.06NSVKK572 pKa = 10.03EE573 pKa = 4.36LKK575 pKa = 9.58TFYY578 pKa = 11.19DD579 pKa = 3.95DD580 pKa = 3.38VLKK583 pKa = 11.22NSFISNIEE591 pKa = 3.97NKK593 pKa = 9.98NDD595 pKa = 3.46LQYY598 pKa = 11.38EE599 pKa = 4.0PLINGLYY606 pKa = 10.59NKK608 pKa = 9.03MLIMRR613 pKa = 11.84NSIDD617 pKa = 3.56RR618 pKa = 11.84IVRR621 pKa = 11.84NKK623 pKa = 10.81DD624 pKa = 2.81FDD626 pKa = 5.74IIDD629 pKa = 3.78AMNDD633 pKa = 3.02MRR635 pKa = 11.84LDD637 pKa = 3.55NPEE640 pKa = 4.39AYY642 pKa = 10.25LEE644 pKa = 4.45KK645 pKa = 10.32IKK647 pKa = 11.09NSNKK651 pKa = 9.66PLSNLNDD658 pKa = 3.52MFNTAKK664 pKa = 10.45KK665 pKa = 9.98RR666 pKa = 11.84IKK668 pKa = 10.04EE669 pKa = 3.81INEE672 pKa = 3.75YY673 pKa = 10.18NSEE676 pKa = 4.21YY677 pKa = 10.74FQDD680 pKa = 5.08IYY682 pKa = 11.82DD683 pKa = 4.16FDD685 pKa = 3.91NFSNFRR691 pKa = 11.84PYY693 pKa = 10.48EE694 pKa = 3.91SYY696 pKa = 11.24YY697 pKa = 10.43RR698 pKa = 11.84AEE700 pKa = 4.97LSTQIDD706 pKa = 4.15NLDD709 pKa = 4.24MIRR712 pKa = 11.84GAYY715 pKa = 7.86WRR717 pKa = 11.84DD718 pKa = 3.4PQKK721 pKa = 9.81EE722 pKa = 4.44TEE724 pKa = 4.08MLQYY728 pKa = 9.37WW729 pKa = 3.71

Molecular weight: 86.19 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	729	729	729	729.0	86.19

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
2.881 ± 0.0	0.0 ± 0.0	6.036 ± 0.0	4.664 ± 0.0	6.036 ± 0.0	4.252 ± 0.0	2.606 ± 0.0	10.151 ± 0.0	10.014 ± 0.0	9.465 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
3.292 ± 0.0	8.093 ± 0.0	2.881 ± 0.0	2.195 ± 0.0	4.801 ± 0.0	6.31 ± 0.0	5.487 ± 0.0	3.704 ± 0.0	1.097 ± 0.0	6.036 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski