Luffa yellow mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7T4A3|Q7T4A3_9GEMI Capsid protein OS=Luffa yellow mosaic virus OX=207240 GN=AV1 PE=3 SV=1
MM1 pKa = 7.68PRR3 pKa = 11.84TNQFQVKK10 pKa = 9.47AKK12 pKa = 10.56NIFLTFPKK20 pKa = 10.39CPIPKK25 pKa = 9.62EE26 pKa = 4.02HH27 pKa = 6.55MLEE30 pKa = 3.88LLRR33 pKa = 11.84NISCPSDD40 pKa = 3.14KK41 pKa = 11.06LFIRR45 pKa = 11.84VSQEE49 pKa = 3.19KK50 pKa = 9.64HH51 pKa = 4.78QDD53 pKa = 3.22GSLHH57 pKa = 5.16IHH59 pKa = 6.91ALIQFKK65 pKa = 10.38GKK67 pKa = 9.81CQFRR71 pKa = 11.84NPRR74 pKa = 11.84HH75 pKa = 6.12FDD77 pKa = 3.1VTHH80 pKa = 7.25PNTSTQYY87 pKa = 10.45HH88 pKa = 7.05PNFQGAKK95 pKa = 9.08SSSDD99 pKa = 3.06VKK101 pKa = 11.23SYY103 pKa = 10.8IEE105 pKa = 4.55KK106 pKa = 10.86DD107 pKa = 2.8GDD109 pKa = 4.02YY110 pKa = 10.58IDD112 pKa = 4.14WGQFQIDD119 pKa = 3.44GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 3.91AAAEE136 pKa = 3.92ALNAGSKK143 pKa = 9.49EE144 pKa = 3.75AALSIIRR151 pKa = 11.84EE152 pKa = 4.35KK153 pKa = 10.99LPKK156 pKa = 10.13DD157 pKa = 3.78YY158 pKa = 10.83IFQFHH163 pKa = 6.7NLNSNLDD170 pKa = 4.05RR171 pKa = 11.84IFAPPLEE178 pKa = 4.42VFVCPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 3.87EE195 pKa = 4.25LEE197 pKa = 4.08VWVSEE202 pKa = 4.19NVRR205 pKa = 11.84DD206 pKa = 3.6AAARR210 pKa = 11.84PWRR213 pKa = 11.84PKK215 pKa = 10.55SIVIEE220 pKa = 4.33GDD222 pKa = 3.1SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 3.88LSPRR249 pKa = 11.84VYY251 pKa = 11.16NNEE254 pKa = 2.91AWYY257 pKa = 10.8NVIDD261 pKa = 5.29DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.47YY287 pKa = 10.28GKK289 pKa = 9.35PVMIKK294 pKa = 10.55GGIPTIFLCNKK305 pKa = 9.29GPNSSYY311 pKa = 11.07KK312 pKa = 10.45EE313 pKa = 3.93YY314 pKa = 10.94LDD316 pKa = 3.87EE317 pKa = 4.82EE318 pKa = 4.69KK319 pKa = 11.12NSALKK324 pKa = 10.0QWAIKK329 pKa = 10.25NAVFIQLEE337 pKa = 4.22EE338 pKa = 3.96PLYY341 pKa = 10.88SGRR344 pKa = 11.84SNNAPTEE351 pKa = 4.13EE352 pKa = 4.31EE353 pKa = 4.72EE354 pKa = 4.14EE355 pKa = 4.46HH356 pKa = 5.49QTQTDD361 pKa = 3.12

Molecular weight: 41.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7T4A4|Q7T4A4_9GEMI Protein V2 OS=Luffa yellow mosaic virus OX=207240 GN=AV2 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.87IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 9.72VSRR30 pKa = 11.84AVVPIARR37 pKa = 11.84VTKK40 pKa = 10.21GKK42 pKa = 10.45AWTNRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.8PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.06SRR80 pKa = 11.84HH81 pKa = 5.57DD82 pKa = 3.47VSHH85 pKa = 6.93IGKK88 pKa = 9.37VMCISDD94 pKa = 3.67VTRR97 pKa = 11.84GTGLTHH103 pKa = 7.09RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.56NHH131 pKa = 5.33TNSVIFFLVRR141 pKa = 11.84DD142 pKa = 4.02RR143 pKa = 11.84RR144 pKa = 11.84PTGTPQDD151 pKa = 3.67FGEE154 pKa = 4.38VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.61NMHH172 pKa = 7.0RR173 pKa = 11.84DD174 pKa = 3.31RR175 pKa = 11.84YY176 pKa = 8.78QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.6ATVTGGTYY191 pKa = 10.6ASRR194 pKa = 11.84EE195 pKa = 3.76QALVRR200 pKa = 11.84KK201 pKa = 8.27FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.34DD252 pKa = 3.32SVTNN256 pKa = 3.98

Molecular weight: 29.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1740

132

361

217.5

24.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.31 ± 0.616

2.356 ± 0.388

4.655 ± 0.359

4.713 ± 0.746

4.138 ± 0.372

5.402 ± 0.182

3.966 ± 0.585

6.322 ± 0.662

5.805 ± 0.533

6.782 ± 0.794

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.299 ± 0.329

5.92 ± 0.284

5.287 ± 0.37

3.736 ± 0.533

7.069 ± 0.815

8.563 ± 0.776

5.805 ± 0.639

7.356 ± 0.943

1.494 ± 0.153

4.023 ± 0.46
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski