Culex mononega-like virus 2

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z2RT68|A0A1Z2RT68_9VIRU Glycoprotein OS=Culex mononega-like virus 2 OX=2010272 PE=4 SV=1
MM1 pKa = 7.91KK2 pKa = 10.08MEE4 pKa = 3.75VHH6 pKa = 6.72LLVFLSFVALSAGFIAYY23 pKa = 8.55DD24 pKa = 3.82CNGSRR29 pKa = 11.84IQKK32 pKa = 5.62TTVSLVEE39 pKa = 4.29TPSCTFEE46 pKa = 4.41LEE48 pKa = 4.17NVTEE52 pKa = 4.33SEE54 pKa = 4.23VAVAVTQSALTQEE67 pKa = 4.13IPFYY71 pKa = 10.59RR72 pKa = 11.84CLIVAYY78 pKa = 9.28HH79 pKa = 6.35HH80 pKa = 6.77IWRR83 pKa = 11.84CGSVIDD89 pKa = 4.39TNVQGGDD96 pKa = 3.4YY97 pKa = 11.03AEE99 pKa = 4.79VIRR102 pKa = 11.84TTKK105 pKa = 10.4SEE107 pKa = 4.11CEE109 pKa = 3.49EE110 pKa = 4.24MINHH114 pKa = 6.93KK115 pKa = 10.08KK116 pKa = 10.6YY117 pKa = 9.45KK118 pKa = 9.51TIGGNSVSIDD128 pKa = 3.91LPPGGRR134 pKa = 11.84TSFSYY139 pKa = 10.41TSWGQVKK146 pKa = 10.67DD147 pKa = 3.6SGSCVAGPILYY158 pKa = 10.48SGGTSYY164 pKa = 11.12DD165 pKa = 2.95QHH167 pKa = 5.68TRR169 pKa = 11.84NTRR172 pKa = 11.84LEE174 pKa = 3.75ILYY177 pKa = 8.78TRR179 pKa = 11.84GVGRR183 pKa = 11.84VAVEE187 pKa = 4.12KK188 pKa = 10.69KK189 pKa = 10.64SLIMPNGMQCNVALEE204 pKa = 4.17EE205 pKa = 5.04CEE207 pKa = 3.9LADD210 pKa = 3.71YY211 pKa = 10.49GQVFWKK217 pKa = 10.45QPVPSCQNEE226 pKa = 4.09VEE228 pKa = 4.36EE229 pKa = 4.44QSLVYY234 pKa = 10.16KK235 pKa = 10.91GPATLITNQNTSEE248 pKa = 4.23RR249 pKa = 11.84FIQVSFSGHH258 pKa = 5.81HH259 pKa = 5.22FQIKK263 pKa = 10.46LEE265 pKa = 4.18DD266 pKa = 3.52RR267 pKa = 11.84TTYY270 pKa = 10.06ICGFRR275 pKa = 11.84SFFTEE280 pKa = 3.98HH281 pKa = 6.57PKK283 pKa = 10.92LYY285 pKa = 9.02ITLLDD290 pKa = 3.87LSYY293 pKa = 11.27PDD295 pKa = 5.21FPLKK299 pKa = 10.78EE300 pKa = 4.08NTGTDD305 pKa = 3.88VNMMNYY311 pKa = 9.47INSKK315 pKa = 8.94LVYY318 pKa = 9.84SMRR321 pKa = 11.84HH322 pKa = 4.85IKK324 pKa = 8.98EE325 pKa = 3.88QVMTLYY331 pKa = 10.91RR332 pKa = 11.84LFEE335 pKa = 4.3LEE337 pKa = 3.86RR338 pKa = 11.84CLMQNRR344 pKa = 11.84ITSNLLTLAILSPRR358 pKa = 11.84EE359 pKa = 3.51FAYY362 pKa = 10.18QYY364 pKa = 10.3YY365 pKa = 10.35GEE367 pKa = 4.56PGYY370 pKa = 8.47TAVVRR375 pKa = 11.84GEE377 pKa = 4.15VVHH380 pKa = 7.03IARR383 pKa = 11.84CTPVPVTPRR392 pKa = 11.84KK393 pKa = 8.5TEE395 pKa = 3.64LCYY398 pKa = 11.04NEE400 pKa = 5.24LPVSFNNKK408 pKa = 9.16SLFMTPRR415 pKa = 11.84SRR417 pKa = 11.84ILMEE421 pKa = 4.4IGTVVEE427 pKa = 4.73CATDD431 pKa = 3.16VGPQFKK437 pKa = 8.67TANRR441 pKa = 11.84WITMVSHH448 pKa = 6.51GLISVEE454 pKa = 4.04KK455 pKa = 9.57PKK457 pKa = 10.46IITPDD462 pKa = 4.4PITYY466 pKa = 10.04HH467 pKa = 6.68FEE469 pKa = 3.99TLEE472 pKa = 4.11DD473 pKa = 3.73MLTGGLYY480 pKa = 9.95TKK482 pKa = 8.99EE483 pKa = 4.04TIANYY488 pKa = 8.18QQILTSPMEE497 pKa = 3.93EE498 pKa = 4.43SILSSRR504 pKa = 11.84VTTAIRR510 pKa = 11.84GGEE513 pKa = 4.3SLPSGYY519 pKa = 9.15PASNIFSSYY528 pKa = 10.44DD529 pKa = 3.22IEE531 pKa = 4.19RR532 pKa = 11.84LRR534 pKa = 11.84VKK536 pKa = 9.22VTSGLVHH543 pKa = 6.49TMDD546 pKa = 4.18KK547 pKa = 11.04LLVLGNWFSVFVMIGWAINTLISIINCGLNYY578 pKa = 10.5YY579 pKa = 7.93VVKK582 pKa = 10.42PEE584 pKa = 3.84TSGIFALLTCCFSAIFQVVKK604 pKa = 10.55NRR606 pKa = 11.84QLGNPEE612 pKa = 3.77EE613 pKa = 4.44TKK615 pKa = 10.12TLSAVVQIDD624 pKa = 3.51AEE626 pKa = 4.41GRR628 pKa = 11.84EE629 pKa = 4.3EE630 pKa = 3.87PSHH633 pKa = 6.03NKK635 pKa = 9.51FYY637 pKa = 11.02KK638 pKa = 10.4

Molecular weight: 71.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z2RT27|A0A1Z2RT27_9VIRU Uncharacterized protein OS=Culex mononega-like virus 2 OX=2010272 PE=4 SV=1
MM1 pKa = 7.48NYY3 pKa = 7.67ITPTILVAVIVIAHH17 pKa = 5.68TVAIRR22 pKa = 11.84SLSYY26 pKa = 11.64DD27 pKa = 3.05MTDD30 pKa = 3.05RR31 pKa = 11.84VPVIALINDD40 pKa = 3.49HH41 pKa = 6.88ASWFMKK47 pKa = 9.54LTVSPVTINFYY58 pKa = 10.09ATLTSFFATLALMGVVAKK76 pKa = 10.36NWRR79 pKa = 11.84AYY81 pKa = 10.3VRR83 pKa = 11.84DD84 pKa = 3.37IQRR87 pKa = 11.84GVVRR91 pKa = 11.84AEE93 pKa = 3.54VLMRR97 pKa = 11.84SAA99 pKa = 4.92

Molecular weight: 11.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3855

86

2079

642.5

73.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.811 ± 0.572

2.387 ± 0.232

4.929 ± 0.819

6.978 ± 0.467

4.099 ± 0.248

5.785 ± 0.421

2.464 ± 0.144

6.304 ± 0.426

5.759 ± 0.662

9.183 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.32 ± 0.178

4.047 ± 0.438

4.202 ± 0.244

3.398 ± 0.156

5.914 ± 0.436

6.848 ± 0.69

6.667 ± 0.887

6.693 ± 0.566

1.167 ± 0.162

4.047 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski