Amino acid dipepetide frequency for Pseudomonas pohangensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.284AlaAla: 13.284 ± 0.146	1.35AlaCys: 1.35 ± 0.037	5.958AlaAsp: 5.958 ± 0.075	7.347AlaGlu: 7.347 ± 0.101	3.695AlaPhe: 3.695 ± 0.063	9.486AlaGly: 9.486 ± 0.1	2.083AlaHis: 2.083 ± 0.038	5.316AlaIle: 5.316 ± 0.08	3.529AlaLys: 3.529 ± 0.075	13.379AlaLeu: 13.379 ± 0.155	2.943AlaMet: 2.943 ± 0.055	2.969AlaAsn: 2.969 ± 0.056	4.404AlaPro: 4.404 ± 0.071	4.939AlaGln: 4.939 ± 0.083	7.195AlaArg: 7.195 ± 0.096	6.178AlaSer: 6.178 ± 0.08	4.722AlaThr: 4.722 ± 0.102	7.213AlaVal: 7.213 ± 0.085	1.527AlaTrp: 1.527 ± 0.042	2.624AlaTyr: 2.624 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	1.244CysAla: 1.244 ± 0.031	0.164CysCys: 0.164 ± 0.012	0.579CysAsp: 0.579 ± 0.028	0.6CysGlu: 0.6 ± 0.021	0.39CysPhe: 0.39 ± 0.02	0.997CysGly: 0.997 ± 0.03	0.313CysHis: 0.313 ± 0.019	0.516CysIle: 0.516 ± 0.021	0.357CysLys: 0.357 ± 0.019	1.206CysLeu: 1.206 ± 0.037	0.259CysMet: 0.259 ± 0.016	0.35CysAsn: 0.35 ± 0.02	0.576CysPro: 0.576 ± 0.028	0.447CysGln: 0.447 ± 0.021	0.658CysArg: 0.658 ± 0.025	0.726CysSer: 0.726 ± 0.026	0.478CysThr: 0.478 ± 0.021	0.718CysVal: 0.718 ± 0.024	0.165CysTrp: 0.165 ± 0.012	0.287CysTyr: 0.287 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	5.498AspAla: 5.498 ± 0.066	0.636AspCys: 0.636 ± 0.025	2.675AspAsp: 2.675 ± 0.057	3.286AspGlu: 3.286 ± 0.057	2.163AspPhe: 2.163 ± 0.048	4.171AspGly: 4.171 ± 0.084	0.995AspHis: 0.995 ± 0.032	2.689AspIle: 2.689 ± 0.051	2.093AspLys: 2.093 ± 0.049	5.868AspLeu: 5.868 ± 0.079	1.299AspMet: 1.299 ± 0.033	1.711AspAsn: 1.711 ± 0.055	2.79AspPro: 2.79 ± 0.053	2.3AspGln: 2.3 ± 0.049	2.763AspArg: 2.763 ± 0.053	3.053AspSer: 3.053 ± 0.06	2.213AspThr: 2.213 ± 0.057	3.316AspVal: 3.316 ± 0.063	1.049AspTrp: 1.049 ± 0.035	1.703AspTyr: 1.703 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	6.267GluAla: 6.267 ± 0.096	0.467GluCys: 0.467 ± 0.023	2.575GluAsp: 2.575 ± 0.05	3.093GluGlu: 3.093 ± 0.065	1.966GluPhe: 1.966 ± 0.042	3.754GluGly: 3.754 ± 0.061	1.538GluHis: 1.538 ± 0.038	2.897GluIle: 2.897 ± 0.059	2.307GluLys: 2.307 ± 0.053	7.268GluLeu: 7.268 ± 0.081	1.443GluMet: 1.443 ± 0.036	1.669GluAsn: 1.669 ± 0.039	2.381GluPro: 2.381 ± 0.053	4.009GluGln: 4.009 ± 0.077	4.29GluArg: 4.29 ± 0.073	2.854GluSer: 2.854 ± 0.053	2.479GluThr: 2.479 ± 0.05	4.24GluVal: 4.24 ± 0.073	0.716GluTrp: 0.716 ± 0.029	1.224GluTyr: 1.224 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	4.161PheAla: 4.161 ± 0.069	0.525PheCys: 0.525 ± 0.023	2.398PheAsp: 2.398 ± 0.047	2.03PheGlu: 2.03 ± 0.047	1.532PhePhe: 1.532 ± 0.043	3.209PheGly: 3.209 ± 0.06	0.721PheHis: 0.721 ± 0.021	1.915PheIle: 1.915 ± 0.048	1.258PheLys: 1.258 ± 0.033	3.406PheLeu: 3.406 ± 0.053	0.87PheMet: 0.87 ± 0.026	1.372PheAsn: 1.372 ± 0.035	1.55PhePro: 1.55 ± 0.037	1.225PheGln: 1.225 ± 0.035	1.981PheArg: 1.981 ± 0.038	2.754PheSer: 2.754 ± 0.051	1.855PheThr: 1.855 ± 0.043	2.44PheVal: 2.44 ± 0.047	0.564PheTrp: 0.564 ± 0.029	1.066PheTyr: 1.066 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	7.123GlyAla: 7.123 ± 0.08	1.066GlyCys: 1.066 ± 0.035	3.728GlyAsp: 3.728 ± 0.065	4.598GlyGlu: 4.598 ± 0.077	3.267GlyPhe: 3.267 ± 0.058	5.892GlyGly: 5.892 ± 0.08	1.833GlyHis: 1.833 ± 0.039	4.275GlyIle: 4.275 ± 0.07	3.714GlyLys: 3.714 ± 0.063	9.329GlyLeu: 9.329 ± 0.101	2.384GlyMet: 2.384 ± 0.053	2.595GlyAsn: 2.595 ± 0.063	2.533GlyPro: 2.533 ± 0.054	3.632GlyGln: 3.632 ± 0.055	4.608GlyArg: 4.608 ± 0.064	4.778GlySer: 4.778 ± 0.078	3.584GlyThr: 3.584 ± 0.105	5.729GlyVal: 5.729 ± 0.085	1.399GlyTrp: 1.399 ± 0.032	2.43GlyTyr: 2.43 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	2.273HisAla: 2.273 ± 0.046	0.351HisCys: 0.351 ± 0.017	1.047HisAsp: 1.047 ± 0.032	1.115HisGlu: 1.115 ± 0.031	1.004HisPhe: 1.004 ± 0.035	1.928HisGly: 1.928 ± 0.045	0.581HisHis: 0.581 ± 0.023	1.004HisIle: 1.004 ± 0.031	0.722HisLys: 0.722 ± 0.027	2.556HisLeu: 2.556 ± 0.048	0.533HisMet: 0.533 ± 0.022	0.746HisAsn: 0.746 ± 0.023	1.342HisPro: 1.342 ± 0.037	0.894HisGln: 0.894 ± 0.028	1.219HisArg: 1.219 ± 0.038	1.306HisSer: 1.306 ± 0.039	0.9HisThr: 0.9 ± 0.03	1.303HisVal: 1.303 ± 0.031	0.44HisTrp: 0.44 ± 0.021	0.706HisTyr: 0.706 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.77IleAla: 5.77 ± 0.076	0.57IleCys: 0.57 ± 0.025	3.201IleAsp: 3.201 ± 0.058	3.446IleGlu: 3.446 ± 0.067	1.589IlePhe: 1.589 ± 0.043	4.398IleGly: 4.398 ± 0.077	0.976IleHis: 0.976 ± 0.029	2.187IleIle: 2.187 ± 0.057	1.842IleLys: 1.842 ± 0.043	4.458IleLeu: 4.458 ± 0.067	0.913IleMet: 0.913 ± 0.034	1.853IleAsn: 1.853 ± 0.043	2.382IlePro: 2.382 ± 0.041	1.7IleGln: 1.7 ± 0.039	2.975IleArg: 2.975 ± 0.061	3.256IleSer: 3.256 ± 0.057	2.552IleThr: 2.552 ± 0.052	3.012IleVal: 3.012 ± 0.059	0.548IleTrp: 0.548 ± 0.019	1.28IleTyr: 1.28 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	4.148LysAla: 4.148 ± 0.068	0.233LysCys: 0.233 ± 0.016	1.726LysAsp: 1.726 ± 0.046	1.703LysGlu: 1.703 ± 0.05	0.934LysPhe: 0.934 ± 0.03	2.579LysGly: 2.579 ± 0.057	0.781LysHis: 0.781 ± 0.026	1.738LysIle: 1.738 ± 0.042	1.444LysLys: 1.444 ± 0.049	4.066LysLeu: 4.066 ± 0.069	0.793LysMet: 0.793 ± 0.029	1.136LysAsn: 1.136 ± 0.034	2.198LysPro: 2.198 ± 0.046	1.759LysGln: 1.759 ± 0.048	2.365LysArg: 2.365 ± 0.06	2.015LysSer: 2.015 ± 0.053	1.971LysThr: 1.971 ± 0.046	2.79LysVal: 2.79 ± 0.057	0.383LysTrp: 0.383 ± 0.018	0.774LysTyr: 0.774 ± 0.03	0.0LysXaa: 0.0 ± 0.0
Leu	14.46LeuAla: 14.46 ± 0.167	1.334LeuCys: 1.334 ± 0.037	6.687LeuAsp: 6.687 ± 0.086	6.556LeuGlu: 6.556 ± 0.082	4.344LeuPhe: 4.344 ± 0.078	9.284LeuGly: 9.284 ± 0.114	2.586LeuHis: 2.586 ± 0.058	5.731LeuIle: 5.731 ± 0.099	4.332LeuLys: 4.332 ± 0.073	15.516LeuLeu: 15.516 ± 0.211	2.499LeuMet: 2.499 ± 0.059	3.608LeuAsn: 3.608 ± 0.058	6.794LeuPro: 6.794 ± 0.091	6.074LeuGln: 6.074 ± 0.091	7.519LeuArg: 7.519 ± 0.097	6.775LeuSer: 6.775 ± 0.097	5.417LeuThr: 5.417 ± 0.075	7.578LeuVal: 7.578 ± 0.091	1.518LeuTrp: 1.518 ± 0.036	2.614LeuTyr: 2.614 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	2.695MetAla: 2.695 ± 0.05	0.183MetCys: 0.183 ± 0.013	1.091MetAsp: 1.091 ± 0.034	1.016MetGlu: 1.016 ± 0.033	0.751MetPhe: 0.751 ± 0.028	1.733MetGly: 1.733 ± 0.045	0.578MetHis: 0.578 ± 0.023	1.13MetIle: 1.13 ± 0.033	0.949MetLys: 0.949 ± 0.031	3.03MetLeu: 3.03 ± 0.054	0.508MetMet: 0.508 ± 0.026	0.852MetAsn: 0.852 ± 0.023	1.412MetPro: 1.412 ± 0.039	1.358MetGln: 1.358 ± 0.033	1.532MetArg: 1.532 ± 0.035	1.726MetSer: 1.726 ± 0.038	1.406MetThr: 1.406 ± 0.035	1.506MetVal: 1.506 ± 0.046	0.203MetTrp: 0.203 ± 0.014	0.419MetTyr: 0.419 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	3.068AsnAla: 3.068 ± 0.055	0.354AsnCys: 0.354 ± 0.016	1.524AsnAsp: 1.524 ± 0.055	1.436AsnGlu: 1.436 ± 0.037	1.132AsnPhe: 1.132 ± 0.034	2.559AsnGly: 2.559 ± 0.062	0.651AsnHis: 0.651 ± 0.023	1.509AsnIle: 1.509 ± 0.04	1.036AsnLys: 1.036 ± 0.034	3.64AsnLeu: 3.64 ± 0.056	0.716AsnMet: 0.716 ± 0.026	1.004AsnAsn: 1.004 ± 0.037	2.207AsnPro: 2.207 ± 0.049	1.474AsnGln: 1.474 ± 0.039	1.856AsnArg: 1.856 ± 0.044	1.727AsnSer: 1.727 ± 0.053	1.472AsnThr: 1.472 ± 0.04	1.778AsnVal: 1.778 ± 0.045	0.528AsnTrp: 0.528 ± 0.021	0.879AsnTyr: 0.879 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	6.121ProAla: 6.121 ± 0.082	0.406ProCys: 0.406 ± 0.021	2.944ProAsp: 2.944 ± 0.049	3.445ProGlu: 3.445 ± 0.055	1.798ProPhe: 1.798 ± 0.04	4.177ProGly: 4.177 ± 0.068	0.977ProHis: 0.977 ± 0.032	1.91ProIle: 1.91 ± 0.042	1.493ProLys: 1.493 ± 0.042	5.829ProLeu: 5.829 ± 0.088	1.197ProMet: 1.197 ± 0.032	1.279ProAsn: 1.279 ± 0.033	1.927ProPro: 1.927 ± 0.045	2.476ProGln: 2.476 ± 0.055	2.503ProArg: 2.503 ± 0.052	2.352ProSer: 2.352 ± 0.048	1.983ProThr: 1.983 ± 0.042	3.854ProVal: 3.854 ± 0.063	0.718ProTrp: 0.718 ± 0.024	1.227ProTyr: 1.227 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	6.001GlnAla: 6.001 ± 0.092	0.367GlnCys: 0.367 ± 0.017	1.932GlnAsp: 1.932 ± 0.046	1.974GlnGlu: 1.974 ± 0.043	1.468GlnPhe: 1.468 ± 0.044	3.286GlnGly: 3.286 ± 0.054	1.21GlnHis: 1.21 ± 0.04	2.203GlnIle: 2.203 ± 0.045	1.423GlnLys: 1.423 ± 0.035	6.577GlnLeu: 6.577 ± 0.103	1.185GlnMet: 1.185 ± 0.031	1.134GlnAsn: 1.134 ± 0.032	2.686GlnPro: 2.686 ± 0.05	3.468GlnGln: 3.468 ± 0.083	3.814GlnArg: 3.814 ± 0.077	2.459GlnSer: 2.459 ± 0.052	2.058GlnThr: 2.058 ± 0.057	3.536GlnVal: 3.536 ± 0.06	0.707GlnTrp: 0.707 ± 0.026	1.042GlnTyr: 1.042 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	5.844ArgAla: 5.844 ± 0.082	0.652ArgCys: 0.652 ± 0.029	3.256ArgAsp: 3.256 ± 0.061	4.229ArgGlu: 4.229 ± 0.071	2.717ArgPhe: 2.717 ± 0.058	3.793ArgGly: 3.793 ± 0.058	1.596ArgHis: 1.596 ± 0.038	3.402ArgIle: 3.402 ± 0.052	2.229ArgLys: 2.229 ± 0.047	8.149ArgLeu: 8.149 ± 0.1	1.615ArgMet: 1.615 ± 0.033	1.983ArgAsn: 1.983 ± 0.041	2.679ArgPro: 2.679 ± 0.056	3.493ArgGln: 3.493 ± 0.063	4.026ArgArg: 4.026 ± 0.065	3.516ArgSer: 3.516 ± 0.06	2.462ArgThr: 2.462 ± 0.047	4.202ArgVal: 4.202 ± 0.075	1.033ArgTrp: 1.033 ± 0.032	1.961ArgTyr: 1.961 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	6.17SerAla: 6.17 ± 0.079	0.584SerCys: 0.584 ± 0.023	2.867SerAsp: 2.867 ± 0.062	3.206SerGlu: 3.206 ± 0.061	2.246SerPhe: 2.246 ± 0.047	5.443SerGly: 5.443 ± 0.075	1.259SerHis: 1.259 ± 0.036	2.803SerIle: 2.803 ± 0.054	2.075SerLys: 2.075 ± 0.043	7.101SerLeu: 7.101 ± 0.108	1.547SerMet: 1.547 ± 0.036	1.87SerAsn: 1.87 ± 0.047	2.611SerPro: 2.611 ± 0.051	2.432SerGln: 2.432 ± 0.048	3.576SerArg: 3.576 ± 0.054	3.607SerSer: 3.607 ± 0.073	2.659SerThr: 2.659 ± 0.056	3.753SerVal: 3.753 ± 0.069	0.93SerTrp: 0.93 ± 0.031	1.474SerTyr: 1.474 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	5.095ThrAla: 5.095 ± 0.084	0.501ThrCys: 0.501 ± 0.025	2.391ThrAsp: 2.391 ± 0.054	2.323ThrGlu: 2.323 ± 0.042	1.655ThrPhe: 1.655 ± 0.036	4.062ThrGly: 4.062 ± 0.063	0.942ThrHis: 0.942 ± 0.03	2.062ThrIle: 2.062 ± 0.059	1.056ThrLys: 1.056 ± 0.034	6.227ThrLeu: 6.227 ± 0.106	0.787ThrMet: 0.787 ± 0.026	1.136ThrAsn: 1.136 ± 0.04	2.912ThrPro: 2.912 ± 0.059	1.754ThrGln: 1.754 ± 0.061	2.962ThrArg: 2.962 ± 0.051	2.559ThrSer: 2.559 ± 0.056	2.266ThrThr: 2.266 ± 0.053	3.142ThrVal: 3.142 ± 0.062	0.582ThrTrp: 0.582 ± 0.026	1.254ThrTyr: 1.254 ± 0.062	0.0ThrXaa: 0.0 ± 0.0
Val	7.21ValAla: 7.21 ± 0.089	0.783ValCys: 0.783 ± 0.03	3.775ValAsp: 3.775 ± 0.069	4.238ValGlu: 4.238 ± 0.068	2.553ValPhe: 2.553 ± 0.05	4.769ValGly: 4.769 ± 0.059	1.342ValHis: 1.342 ± 0.038	3.839ValIle: 3.839 ± 0.064	2.397ValLys: 2.397 ± 0.06	8.013ValLeu: 8.013 ± 0.097	1.698ValMet: 1.698 ± 0.047	2.099ValAsn: 2.099 ± 0.046	3.171ValPro: 3.171 ± 0.057	2.694ValGln: 2.694 ± 0.052	4.03ValArg: 4.03 ± 0.064	4.161ValSer: 4.161 ± 0.059	3.419ValThr: 3.419 ± 0.087	4.93ValVal: 4.93 ± 0.079	0.869ValTrp: 0.869 ± 0.037	1.524ValTyr: 1.524 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.151TrpAla: 1.151 ± 0.036	0.169TrpCys: 0.169 ± 0.013	0.617TrpAsp: 0.617 ± 0.026	0.608TrpGlu: 0.608 ± 0.026	0.559TrpPhe: 0.559 ± 0.021	0.929TrpGly: 0.929 ± 0.032	0.41TrpHis: 0.41 ± 0.019	0.711TrpIle: 0.711 ± 0.023	0.462TrpLys: 0.462 ± 0.021	2.407TrpLeu: 2.407 ± 0.052	0.374TrpMet: 0.374 ± 0.02	0.431TrpAsn: 0.431 ± 0.021	0.712TrpPro: 0.712 ± 0.026	1.006TrpGln: 1.006 ± 0.033	1.045TrpArg: 1.045 ± 0.033	0.826TrpSer: 0.826 ± 0.032	0.578TrpThr: 0.578 ± 0.028	0.896TrpVal: 0.896 ± 0.028	0.262TrpTrp: 0.262 ± 0.018	0.407TrpTyr: 0.407 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.561TyrAla: 2.561 ± 0.053	0.335TyrCys: 0.335 ± 0.017	1.3TyrAsp: 1.3 ± 0.037	1.185TyrGlu: 1.185 ± 0.032	1.068TyrPhe: 1.068 ± 0.029	2.047TyrGly: 2.047 ± 0.046	0.598TyrHis: 0.598 ± 0.025	1.068TyrIle: 1.068 ± 0.036	0.82TyrLys: 0.82 ± 0.027	3.202TyrLeu: 3.202 ± 0.054	0.514TyrMet: 0.514 ± 0.02	0.752TyrAsn: 0.752 ± 0.027	1.353TyrPro: 1.353 ± 0.04	1.393TyrGln: 1.393 ± 0.041	1.921TyrArg: 1.921 ± 0.042	1.584TyrSer: 1.584 ± 0.042	1.196TyrThr: 1.196 ± 0.047	1.578TyrVal: 1.578 ± 0.042	0.419TyrTrp: 0.419 ± 0.019	0.7TyrTyr: 0.7 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3431 proteins (1117736 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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