Suicoccus acidiformans
Average proteome isoelectric point is 5.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2209 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A347WJM2|A0A347WJM2_9LACT Uncharacterized protein OS=Suicoccus acidiformans OX=2036206 GN=CL176_04305 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 10.53 KK3 pKa = 10.41 LLLLSASMLALSSQGFAPSVHH24 pKa = 6.11 AQNNADD30 pKa = 3.73 VMAVTQTQQSQIQTDD45 pKa = 3.88 YY46 pKa = 11.27 DD47 pKa = 3.41 QAIQSFQSQYY57 pKa = 10.6 PNTHH61 pKa = 5.55 ITDD64 pKa = 3.38 VDD66 pKa = 3.27 IHH68 pKa = 6.0 LHH70 pKa = 5.09 QEE72 pKa = 3.7 TGHH75 pKa = 5.5 FVVRR79 pKa = 11.84 VLGADD84 pKa = 3.35 QSQEE88 pKa = 4.11 YY89 pKa = 10.07 QLITYY94 pKa = 10.2 NGEE97 pKa = 3.63 QFNASRR103 pKa = 11.84 PLEE106 pKa = 4.28 EE107 pKa = 5.33 ANSTSQTLNPAEE119 pKa = 4.27 LASLEE124 pKa = 4.45 TVTQNAIQQAQSDD137 pKa = 4.24 KK138 pKa = 11.01 AVNWSLEE145 pKa = 3.92 GHH147 pKa = 6.85 EE148 pKa = 4.96 DD149 pKa = 3.25 TDD151 pKa = 3.73 APRR154 pKa = 11.84 WDD156 pKa = 3.33 VTFIEE161 pKa = 5.18 DD162 pKa = 3.61 NQEE165 pKa = 3.64 KK166 pKa = 10.17 NVAVDD171 pKa = 3.36 ATTGEE176 pKa = 4.75 VIDD179 pKa = 4.28 STQDD183 pKa = 3.01 SATQEE188 pKa = 4.27 SADD191 pKa = 3.8 TQQAPNASQNTDD203 pKa = 2.45 QDD205 pKa = 3.56 RR206 pKa = 11.84 SDD208 pKa = 3.78 NSDD211 pKa = 2.59 ARR213 pKa = 11.84 HH214 pKa = 6.77 FYY216 pKa = 10.01 RR217 pKa = 11.84 QFYY220 pKa = 10.45 SDD222 pKa = 3.8 YY223 pKa = 9.96 NGTRR227 pKa = 11.84 PTVRR231 pKa = 11.84 DD232 pKa = 2.96 AGYY235 pKa = 10.36 QIRR238 pKa = 11.84 NTPSNSSQTTGPATSTVGQYY258 pKa = 11.37 DD259 pKa = 3.73 DD260 pKa = 6.13 DD261 pKa = 5.1 DD262 pKa = 3.85 WDD264 pKa = 5.68 DD265 pKa = 3.93 YY266 pKa = 12.15 DD267 pKa = 6.67 DD268 pKa = 4.92 YY269 pKa = 12.0 DD270 pKa = 6.52 DD271 pKa = 5.65 YY272 pKa = 12.14 DD273 pKa = 6.15 DD274 pKa = 6.6 YY275 pKa = 12.14 DD276 pKa = 5.63 DD277 pKa = 6.14 DD278 pKa = 3.94 WDD280 pKa = 6.21 DD281 pKa = 3.97 YY282 pKa = 12.14 DD283 pKa = 6.05 DD284 pKa = 4.65 YY285 pKa = 12.01 NDD287 pKa = 3.53 YY288 pKa = 11.54 DD289 pKa = 5.69 DD290 pKa = 6.13 YY291 pKa = 12.07 DD292 pKa = 6.1 GDD294 pKa = 4.67 DD295 pKa = 3.78 DD296 pKa = 4.58
Molecular weight: 33.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.617
IPC_protein 3.668
Toseland 3.427
ProMoST 3.834
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.656
Rodwell 3.49
Grimsley 3.325
Solomon 3.668
Lehninger 3.63
Nozaki 3.795
DTASelect 4.101
Thurlkill 3.49
EMBOSS 3.656
Sillero 3.795
Patrickios 0.846
IPC_peptide 3.668
IPC2_peptide 3.77
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|A0A347WHK8|A0A347WHK8_9LACT Guanine permease OS=Suicoccus acidiformans OX=2036206 GN=CL176_00155 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.67 QPKK8 pKa = 8.89 KK9 pKa = 7.7 RR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 7.95 KK14 pKa = 8.03 VHH16 pKa = 5.72 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTKK25 pKa = 10.14 NGRR28 pKa = 11.84 NVLKK32 pKa = 10.51 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.59 GRR39 pKa = 11.84 KK40 pKa = 8.75 KK41 pKa = 10.39 LASS44 pKa = 3.43
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.416
IPC2_protein 11.111
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.632
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.486
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.193
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.986
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2209
0
2209
678505
28
3877
307.2
34.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.106 ± 0.057
0.508 ± 0.013
5.956 ± 0.047
7.785 ± 0.06
3.978 ± 0.034
6.637 ± 0.05
2.097 ± 0.025
7.225 ± 0.052
4.97 ± 0.05
9.656 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.601 ± 0.031
4.359 ± 0.038
3.646 ± 0.053
4.821 ± 0.047
4.449 ± 0.042
5.785 ± 0.038
5.546 ± 0.054
6.992 ± 0.044
0.876 ± 0.017
4.007 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here