Aurantiacibacter spongiae
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2690 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N5CPX8|A0A3N5CPX8_9SPHN Thiolase family protein OS=Aurantiacibacter spongiae OX=2488860 GN=EG799_02660 PE=3 SV=1
MM1 pKa = 7.33 TNISRR6 pKa = 11.84 NLILGCSLLALSACGPDD23 pKa = 4.44 EE24 pKa = 4.08 IASPGTGGDD33 pKa = 3.45 IIINNPSPTPSPTPTPTPTSGGVTAAAGCPTIADD67 pKa = 3.79 AQGLTDD73 pKa = 4.27 EE74 pKa = 4.96 GTISGPTGEE83 pKa = 4.53 YY84 pKa = 9.35 RR85 pKa = 11.84 VCATPIRR92 pKa = 11.84 FNSSSTLSYY101 pKa = 11.3 VPGLLYY107 pKa = 10.51 RR108 pKa = 11.84 LPGQVSVGTDD118 pKa = 3.56 GGPAPDD124 pKa = 4.25 NSDD127 pKa = 3.54 GFDD130 pKa = 3.59 DD131 pKa = 4.16 TDD133 pKa = 3.66 VEE135 pKa = 4.4 LTIEE139 pKa = 4.37 PGVIIYY145 pKa = 10.64 ASGSSFLNVTRR156 pKa = 11.84 GNSIDD161 pKa = 3.75 ANGTEE166 pKa = 4.41 DD167 pKa = 3.79 RR168 pKa = 11.84 PIIFTSRR175 pKa = 11.84 DD176 pKa = 3.4 NILGLNNDD184 pKa = 3.64 NSSGQWGGVVLSGRR198 pKa = 11.84 APVTDD203 pKa = 4.52 CFQPGATPGTVNCEE217 pKa = 4.05 RR218 pKa = 11.84 QVEE221 pKa = 4.6 GAADD225 pKa = 3.69 PNFFGGATPDD235 pKa = 3.85 DD236 pKa = 4.42 SSGSMSYY243 pKa = 10.01 VQIRR247 pKa = 11.84 FSGFVLSGDD256 pKa = 4.13 NEE258 pKa = 4.34 LQSLTTGGVGSGTDD272 pKa = 3.18 LSYY275 pKa = 10.78 IQSVNSSDD283 pKa = 4.32 DD284 pKa = 3.38 GAEE287 pKa = 4.02 FFGGRR292 pKa = 11.84 VNLSHH297 pKa = 7.28 LIVAGAEE304 pKa = 3.94 DD305 pKa = 5.08 DD306 pKa = 5.31 SLDD309 pKa = 3.36 TDD311 pKa = 3.83 TGVKK315 pKa = 10.69 ANFQYY320 pKa = 11.2 VLAAQRR326 pKa = 11.84 SGVGDD331 pKa = 3.51 TMIEE335 pKa = 3.9 ADD337 pKa = 3.88 SSNGLEE343 pKa = 4.42 DD344 pKa = 3.14 QTPRR348 pKa = 11.84 QNTQVANFTFIQRR361 pKa = 11.84 RR362 pKa = 11.84 SGDD365 pKa = 3.2 QVIRR369 pKa = 11.84 LRR371 pKa = 11.84 GGTDD375 pKa = 2.93 YY376 pKa = 11.6 GLYY379 pKa = 9.99 NGVVIDD385 pKa = 4.1 QSSSGTPCIRR395 pKa = 11.84 IDD397 pKa = 3.6 DD398 pKa = 4.08 QEE400 pKa = 4.46 TIRR403 pKa = 11.84 AANAGLDD410 pKa = 3.76 DD411 pKa = 4.03 VGPPRR416 pKa = 11.84 FNSVALDD423 pKa = 3.52 CQTNFRR429 pKa = 11.84 AGSDD433 pKa = 3.58 GVTVADD439 pKa = 3.91 VEE441 pKa = 4.92 AILTSGTGNTLDD453 pKa = 4.16 YY454 pKa = 11.4 DD455 pKa = 3.74 NTIQNGYY462 pKa = 10.3 LNGANEE468 pKa = 4.68 DD469 pKa = 3.71 GYY471 pKa = 11.12 MPIFDD476 pKa = 4.02 VTSLSSFFEE485 pKa = 4.18 SASFIGAVSASNDD498 pKa = 2.81 WTQGWTCDD506 pKa = 3.18 SAAITFGNNTGNCLTLPVYY525 pKa = 10.6
Molecular weight: 54.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.732
IPC_protein 3.77
Toseland 3.528
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.592
Grimsley 3.439
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.177
Thurlkill 3.592
EMBOSS 3.732
Sillero 3.897
Patrickios 1.888
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|A0A3N5CVL0|A0A3N5CVL0_9SPHN ATP synthase gamma chain OS=Aurantiacibacter spongiae OX=2488860 GN=atpG PE=3 SV=1
MM1 pKa = 7.22 TFSHH5 pKa = 7.47 PRR7 pKa = 11.84 RR8 pKa = 11.84 QPRR11 pKa = 11.84 RR12 pKa = 11.84 SRR14 pKa = 11.84 RR15 pKa = 11.84 SRR17 pKa = 11.84 EE18 pKa = 3.49 PRR20 pKa = 11.84 PRR22 pKa = 11.84 RR23 pKa = 11.84 LRR25 pKa = 11.84 TRR27 pKa = 11.84 PRR29 pKa = 11.84 ANPRR33 pKa = 11.84 PPRR36 pKa = 11.84 SATTVLPARR45 pKa = 11.84 SPDD48 pKa = 3.21 RR49 pKa = 11.84 ARR51 pKa = 11.84 PARR54 pKa = 11.84 PAVRR58 pKa = 11.84 RR59 pKa = 11.84 HH60 pKa = 6.08 RR61 pKa = 11.84 RR62 pKa = 11.84 RR63 pKa = 11.84 TWPKK67 pKa = 9.07 GRR69 pKa = 11.84 TGRR72 pKa = 11.84 RR73 pKa = 3.22
Molecular weight: 8.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.502
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.427
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.135
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.178
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2690
0
2690
867789
41
1865
322.6
34.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.965 ± 0.067
0.836 ± 0.014
6.566 ± 0.037
6.144 ± 0.048
3.533 ± 0.028
8.963 ± 0.048
1.916 ± 0.022
4.743 ± 0.031
2.514 ± 0.039
9.758 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.475 ± 0.023
2.479 ± 0.025
5.202 ± 0.037
3.033 ± 0.029
7.841 ± 0.05
5.137 ± 0.036
5.191 ± 0.029
7.094 ± 0.035
1.43 ± 0.019
2.182 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here