Amino acid dipepetide frequency for Pseudokineococcus lusitanus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	28.74AlaAla: 28.74 ± 0.31	0.903AlaCys: 0.903 ± 0.029	10.417AlaAsp: 10.417 ± 0.108	8.624AlaGlu: 8.624 ± 0.1	2.837AlaPhe: 2.837 ± 0.059	16.888AlaGly: 16.888 ± 0.17	2.457AlaHis: 2.457 ± 0.053	1.882AlaIle: 1.882 ± 0.052	1.284AlaLys: 1.284 ± 0.046	17.114AlaLeu: 17.114 ± 0.172	2.256AlaMet: 2.256 ± 0.049	1.254AlaAsn: 1.254 ± 0.042	9.953AlaPro: 9.953 ± 0.146	3.279AlaGln: 3.279 ± 0.056	12.506AlaArg: 12.506 ± 0.141	6.275AlaSer: 6.275 ± 0.091	8.48AlaThr: 8.48 ± 0.123	16.878AlaVal: 16.878 ± 0.147	2.121AlaTrp: 2.121 ± 0.048	2.04AlaTyr: 2.04 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.783CysAla: 0.783 ± 0.026	0.077CysCys: 0.077 ± 0.008	0.372CysAsp: 0.372 ± 0.018	0.288CysGlu: 0.288 ± 0.016	0.138CysPhe: 0.138 ± 0.011	0.714CysGly: 0.714 ± 0.028	0.132CysHis: 0.132 ± 0.011	0.071CysIle: 0.071 ± 0.008	0.058CysLys: 0.058 ± 0.008	0.541CysLeu: 0.541 ± 0.02	0.07CysMet: 0.07 ± 0.008	0.074CysAsn: 0.074 ± 0.008	0.375CysPro: 0.375 ± 0.019	0.102CysGln: 0.102 ± 0.009	0.466CysArg: 0.466 ± 0.021	0.315CysSer: 0.315 ± 0.016	0.374CysThr: 0.374 ± 0.019	0.53CysVal: 0.53 ± 0.02	0.09CysTrp: 0.09 ± 0.009	0.104CysTyr: 0.104 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	10.432AspAla: 10.432 ± 0.12	0.268AspCys: 0.268 ± 0.014	5.568AspAsp: 5.568 ± 0.084	4.284AspGlu: 4.284 ± 0.066	1.116AspPhe: 1.116 ± 0.034	8.452AspGly: 8.452 ± 0.099	1.291AspHis: 1.291 ± 0.041	0.901AspIle: 0.901 ± 0.03	0.514AspLys: 0.514 ± 0.024	7.609AspLeu: 7.609 ± 0.092	0.561AspMet: 0.561 ± 0.022	0.547AspAsn: 0.547 ± 0.022	4.843AspPro: 4.843 ± 0.071	1.213AspGln: 1.213 ± 0.033	4.957AspArg: 4.957 ± 0.071	1.496AspSer: 1.496 ± 0.043	2.186AspThr: 2.186 ± 0.05	8.418AspVal: 8.418 ± 0.093	0.692AspTrp: 0.692 ± 0.025	0.87AspTyr: 0.87 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	7.367GluAla: 7.367 ± 0.099	0.218GluCys: 0.218 ± 0.015	3.626GluAsp: 3.626 ± 0.058	3.445GluGlu: 3.445 ± 0.064	0.743GluPhe: 0.743 ± 0.03	5.064GluGly: 5.064 ± 0.079	1.671GluHis: 1.671 ± 0.04	1.046GluIle: 1.046 ± 0.036	0.683GluLys: 0.683 ± 0.027	5.623GluLeu: 5.623 ± 0.078	0.691GluMet: 0.691 ± 0.022	0.585GluAsn: 0.585 ± 0.023	3.345GluPro: 3.345 ± 0.076	2.162GluGln: 2.162 ± 0.047	5.405GluArg: 5.405 ± 0.08	1.529GluSer: 1.529 ± 0.045	2.299GluThr: 2.299 ± 0.053	6.457GluVal: 6.457 ± 0.071	0.52GluTrp: 0.52 ± 0.022	0.662GluTyr: 0.662 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	2.897PheAla: 2.897 ± 0.054	0.16PheCys: 0.16 ± 0.013	1.46PheAsp: 1.46 ± 0.041	0.994PheGlu: 0.994 ± 0.032	0.62PhePhe: 0.62 ± 0.026	2.18PheGly: 2.18 ± 0.052	0.339PheHis: 0.339 ± 0.018	0.322PheIle: 0.322 ± 0.019	0.226PheLys: 0.226 ± 0.015	2.002PheLeu: 2.002 ± 0.052	0.229PheMet: 0.229 ± 0.013	0.334PheAsn: 0.334 ± 0.015	1.044PhePro: 1.044 ± 0.033	0.441PheGln: 0.441 ± 0.021	1.404PheArg: 1.404 ± 0.036	1.029PheSer: 1.029 ± 0.034	1.424PheThr: 1.424 ± 0.043	2.202PheVal: 2.202 ± 0.055	0.317PheTrp: 0.317 ± 0.018	0.408PheTyr: 0.408 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	14.805GlyAla: 14.805 ± 0.138	0.7GlyCys: 0.7 ± 0.023	6.535GlyAsp: 6.535 ± 0.081	5.22GlyGlu: 5.22 ± 0.07	2.255GlyPhe: 2.255 ± 0.056	10.873GlyGly: 10.873 ± 0.142	1.962GlyHis: 1.962 ± 0.048	2.055GlyIle: 2.055 ± 0.059	1.241GlyLys: 1.241 ± 0.04	10.842GlyLeu: 10.842 ± 0.108	1.734GlyMet: 1.734 ± 0.044	1.044GlyAsn: 1.044 ± 0.036	6.482GlyPro: 6.482 ± 0.094	2.312GlyGln: 2.312 ± 0.054	9.535GlyArg: 9.535 ± 0.105	5.317GlySer: 5.317 ± 0.074	7.331GlyThr: 7.331 ± 0.128	10.024GlyVal: 10.024 ± 0.099	1.797GlyTrp: 1.797 ± 0.044	1.841GlyTyr: 1.841 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.425HisAla: 2.425 ± 0.052	0.101HisCys: 0.101 ± 0.01	1.397HisAsp: 1.397 ± 0.034	1.095HisGlu: 1.095 ± 0.032	0.349HisPhe: 0.349 ± 0.018	2.049HisGly: 2.049 ± 0.047	0.623HisHis: 0.623 ± 0.026	0.233HisIle: 0.233 ± 0.014	0.167HisLys: 0.167 ± 0.012	2.497HisLeu: 2.497 ± 0.054	0.162HisMet: 0.162 ± 0.013	0.212HisAsn: 0.212 ± 0.015	1.483HisPro: 1.483 ± 0.041	0.413HisGln: 0.413 ± 0.018	1.894HisArg: 1.894 ± 0.05	0.59HisSer: 0.59 ± 0.021	0.724HisThr: 0.724 ± 0.029	2.118HisVal: 2.118 ± 0.054	0.235HisTrp: 0.235 ± 0.015	0.295HisTyr: 0.295 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	2.395IleAla: 2.395 ± 0.064	0.125IleCys: 0.125 ± 0.011	1.036IleAsp: 1.036 ± 0.035	0.799IleGlu: 0.799 ± 0.028	0.43IlePhe: 0.43 ± 0.022	1.85IleGly: 1.85 ± 0.047	0.25IleHis: 0.25 ± 0.015	0.42IleIle: 0.42 ± 0.022	0.292IleLys: 0.292 ± 0.018	0.952IleLeu: 0.952 ± 0.036	0.201IleMet: 0.201 ± 0.015	0.353IleAsn: 0.353 ± 0.022	0.851IlePro: 0.851 ± 0.029	0.329IleGln: 0.329 ± 0.02	0.97IleArg: 0.97 ± 0.033	1.018IleSer: 1.018 ± 0.03	1.397IleThr: 1.397 ± 0.04	1.146IleVal: 1.146 ± 0.038	0.19IleTrp: 0.19 ± 0.012	0.317IleTyr: 0.317 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	1.325LysAla: 1.325 ± 0.039	0.044LysCys: 0.044 ± 0.007	0.696LysAsp: 0.696 ± 0.03	0.532LysGlu: 0.532 ± 0.025	0.188LysPhe: 0.188 ± 0.013	1.011LysGly: 1.011 ± 0.035	0.236LysHis: 0.236 ± 0.013	0.327LysIle: 0.327 ± 0.02	0.313LysLys: 0.313 ± 0.022	0.832LysLeu: 0.832 ± 0.032	0.165LysMet: 0.165 ± 0.013	0.213LysAsn: 0.213 ± 0.016	0.551LysPro: 0.551 ± 0.025	0.321LysGln: 0.321 ± 0.018	0.754LysArg: 0.754 ± 0.029	0.514LysSer: 0.514 ± 0.028	0.62LysThr: 0.62 ± 0.025	1.161LysVal: 1.161 ± 0.039	0.097LysTrp: 0.097 ± 0.009	0.197LysTyr: 0.197 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	17.831LeuAla: 17.831 ± 0.17	0.529LeuCys: 0.529 ± 0.024	7.535LeuAsp: 7.535 ± 0.093	5.477LeuGlu: 5.477 ± 0.073	1.944LeuPhe: 1.944 ± 0.042	10.495LeuGly: 10.495 ± 0.104	2.02LeuHis: 2.02 ± 0.043	1.102LeuIle: 1.102 ± 0.038	0.914LeuLys: 0.914 ± 0.037	12.959LeuLeu: 12.959 ± 0.151	1.086LeuMet: 1.086 ± 0.034	0.93LeuAsn: 0.93 ± 0.029	7.022LeuPro: 7.022 ± 0.081	2.353LeuGln: 2.353 ± 0.048	9.203LeuArg: 9.203 ± 0.112	4.321LeuSer: 4.321 ± 0.074	5.556LeuThr: 5.556 ± 0.079	13.805LeuVal: 13.805 ± 0.164	1.124LeuTrp: 1.124 ± 0.033	1.251LeuTyr: 1.251 ± 0.035	0.0LeuXaa: 0.0 ± 0.0
Met	2.014MetAla: 2.014 ± 0.043	0.071MetCys: 0.071 ± 0.007	0.684MetAsp: 0.684 ± 0.03	0.462MetGlu: 0.462 ± 0.019	0.23MetPhe: 0.23 ± 0.016	1.073MetGly: 1.073 ± 0.033	0.232MetHis: 0.232 ± 0.014	0.275MetIle: 0.275 ± 0.016	0.157MetLys: 0.157 ± 0.013	1.169MetLeu: 1.169 ± 0.033	0.151MetMet: 0.151 ± 0.013	0.161MetAsn: 0.161 ± 0.011	0.979MetPro: 0.979 ± 0.026	0.306MetGln: 0.306 ± 0.017	1.062MetArg: 1.062 ± 0.029	1.226MetSer: 1.226 ± 0.032	1.42MetThr: 1.42 ± 0.034	1.123MetVal: 1.123 ± 0.028	0.114MetTrp: 0.114 ± 0.01	0.216MetTyr: 0.216 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	1.397AsnAla: 1.397 ± 0.037	0.072AsnCys: 0.072 ± 0.008	0.608AsnAsp: 0.608 ± 0.026	0.452AsnGlu: 0.452 ± 0.023	0.304AsnPhe: 0.304 ± 0.016	1.052AsnGly: 1.052 ± 0.034	0.233AsnHis: 0.233 ± 0.014	0.317AsnIle: 0.317 ± 0.018	0.148AsnLys: 0.148 ± 0.012	1.065AsnLeu: 1.065 ± 0.032	0.141AsnMet: 0.141 ± 0.011	0.249AsnAsn: 0.249 ± 0.013	0.847AsnPro: 0.847 ± 0.032	0.282AsnGln: 0.282 ± 0.019	0.756AsnArg: 0.756 ± 0.022	0.422AsnSer: 0.422 ± 0.019	0.629AsnThr: 0.629 ± 0.026	1.112AsnVal: 1.112 ± 0.036	0.147AsnTrp: 0.147 ± 0.011	0.244AsnTyr: 0.244 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	11.436ProAla: 11.436 ± 0.16	0.279ProCys: 0.279 ± 0.017	4.788ProAsp: 4.788 ± 0.068	3.85ProGlu: 3.85 ± 0.062	1.343ProPhe: 1.343 ± 0.039	7.82ProGly: 7.82 ± 0.106	1.142ProHis: 1.142 ± 0.037	0.672ProIle: 0.672 ± 0.027	0.494ProLys: 0.494 ± 0.021	5.958ProLeu: 5.958 ± 0.084	0.769ProMet: 0.769 ± 0.025	0.502ProAsn: 0.502 ± 0.022	4.44ProPro: 4.44 ± 0.107	1.331ProGln: 1.331 ± 0.039	4.783ProArg: 4.783 ± 0.086	3.526ProSer: 3.526 ± 0.066	4.112ProThr: 4.112 ± 0.078	6.833ProVal: 6.833 ± 0.085	0.951ProTrp: 0.951 ± 0.03	0.891ProTyr: 0.891 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.222GlnAla: 3.222 ± 0.057	0.106GlnCys: 0.106 ± 0.009	1.139GlnAsp: 1.139 ± 0.037	1.171GlnGlu: 1.171 ± 0.036	0.429GlnPhe: 0.429 ± 0.021	2.03GlnGly: 2.03 ± 0.048	0.556GlnHis: 0.556 ± 0.028	0.482GlnIle: 0.482 ± 0.022	0.324GlnLys: 0.324 ± 0.02	2.571GlnLeu: 2.571 ± 0.05	0.403GlnMet: 0.403 ± 0.021	0.28GlnAsn: 0.28 ± 0.016	1.448GlnPro: 1.448 ± 0.038	1.064GlnGln: 1.064 ± 0.034	2.309GlnArg: 2.309 ± 0.052	0.805GlnSer: 0.805 ± 0.029	1.065GlnThr: 1.065 ± 0.031	2.867GlnVal: 2.867 ± 0.056	0.306GlnTrp: 0.306 ± 0.019	0.433GlnTyr: 0.433 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	12.1ArgAla: 12.1 ± 0.133	0.514ArgCys: 0.514 ± 0.025	4.776ArgAsp: 4.776 ± 0.077	4.488ArgGlu: 4.488 ± 0.069	1.754ArgPhe: 1.754 ± 0.044	7.485ArgGly: 7.485 ± 0.092	1.7ArgHis: 1.7 ± 0.044	1.355ArgIle: 1.355 ± 0.037	0.783ArgLys: 0.783 ± 0.029	9.389ArgLeu: 9.389 ± 0.12	1.407ArgMet: 1.407 ± 0.038	0.741ArgAsn: 0.741 ± 0.03	6.007ArgPro: 6.007 ± 0.082	1.913ArgGln: 1.913 ± 0.042	10.083ArgArg: 10.083 ± 0.122	4.111ArgSer: 4.111 ± 0.063	5.501ArgThr: 5.501 ± 0.079	7.812ArgVal: 7.812 ± 0.082	1.676ArgTrp: 1.676 ± 0.044	1.493ArgTyr: 1.493 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	6.506SerAla: 6.506 ± 0.075	0.285SerCys: 0.285 ± 0.016	2.235SerAsp: 2.235 ± 0.045	1.764SerGlu: 1.764 ± 0.045	1.254SerPhe: 1.254 ± 0.032	4.976SerGly: 4.976 ± 0.074	0.71SerHis: 0.71 ± 0.026	0.911SerIle: 0.911 ± 0.03	0.501SerLys: 0.501 ± 0.024	4.369SerLeu: 4.369 ± 0.065	0.793SerMet: 0.793 ± 0.023	0.55SerAsn: 0.55 ± 0.024	3.089SerPro: 3.089 ± 0.061	0.944SerGln: 0.944 ± 0.029	3.638SerArg: 3.638 ± 0.059	2.835SerSer: 2.835 ± 0.067	3.435SerThr: 3.435 ± 0.053	4.138SerVal: 4.138 ± 0.059	0.803SerTrp: 0.803 ± 0.03	0.832SerTyr: 0.832 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	10.062ThrAla: 10.062 ± 0.129	0.366ThrCys: 0.366 ± 0.019	3.523ThrAsp: 3.523 ± 0.067	2.402ThrGlu: 2.402 ± 0.059	1.359ThrPhe: 1.359 ± 0.039	7.118ThrGly: 7.118 ± 0.111	0.892ThrHis: 0.892 ± 0.03	0.996ThrIle: 0.996 ± 0.035	0.645ThrLys: 0.645 ± 0.026	4.98ThrLeu: 4.98 ± 0.072	0.661ThrMet: 0.661 ± 0.023	0.749ThrAsn: 0.749 ± 0.029	4.618ThrPro: 4.618 ± 0.083	1.169ThrGln: 1.169 ± 0.033	3.781ThrArg: 3.781 ± 0.055	3.557ThrSer: 3.557 ± 0.066	5.395ThrThr: 5.395 ± 0.104	5.614ThrVal: 5.614 ± 0.092	0.992ThrTrp: 0.992 ± 0.033	1.166ThrTyr: 1.166 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	16.47ValAla: 16.47 ± 0.14	0.624ValCys: 0.624 ± 0.027	7.76ValAsp: 7.76 ± 0.095	6.329ValGlu: 6.329 ± 0.084	1.976ValPhe: 1.976 ± 0.047	10.04ValGly: 10.04 ± 0.11	2.084ValHis: 2.084 ± 0.044	1.509ValIle: 1.509 ± 0.039	0.978ValLys: 0.978 ± 0.034	13.831ValLeu: 13.831 ± 0.158	1.274ValMet: 1.274 ± 0.038	1.223ValAsn: 1.223 ± 0.035	7.023ValPro: 7.023 ± 0.091	2.249ValGln: 2.249 ± 0.048	8.882ValArg: 8.882 ± 0.101	4.065ValSer: 4.065 ± 0.063	6.28ValThr: 6.28 ± 0.1	16.405ValVal: 16.405 ± 0.166	1.16ValTrp: 1.16 ± 0.031	1.376ValTyr: 1.376 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.771TrpAla: 1.771 ± 0.041	0.118TrpCys: 0.118 ± 0.01	0.931TrpAsp: 0.931 ± 0.035	0.624TrpGlu: 0.624 ± 0.023	0.387TrpPhe: 0.387 ± 0.02	1.071TrpGly: 1.071 ± 0.034	0.317TrpHis: 0.317 ± 0.018	0.2TrpIle: 0.2 ± 0.014	0.173TrpLys: 0.173 ± 0.011	1.501TrpLeu: 1.501 ± 0.041	0.224TrpMet: 0.224 ± 0.014	0.218TrpAsn: 0.218 ± 0.014	0.742TrpPro: 0.742 ± 0.024	0.488TrpGln: 0.488 ± 0.021	1.354TrpArg: 1.354 ± 0.039	0.871TrpSer: 0.871 ± 0.03	0.959TrpThr: 0.959 ± 0.032	1.322TrpVal: 1.322 ± 0.031	0.343TrpTrp: 0.343 ± 0.016	0.283TrpTyr: 0.283 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.208TyrAla: 2.208 ± 0.035	0.114TyrCys: 0.114 ± 0.01	1.129TyrAsp: 1.129 ± 0.034	0.84TyrGlu: 0.84 ± 0.027	0.379TyrPhe: 0.379 ± 0.02	1.666TyrGly: 1.666 ± 0.039	0.215TyrHis: 0.215 ± 0.014	0.245TyrIle: 0.245 ± 0.017	0.202TyrLys: 0.202 ± 0.014	1.652TyrLeu: 1.652 ± 0.039	0.147TyrMet: 0.147 ± 0.014	0.258TyrAsn: 0.258 ± 0.017	0.778TyrPro: 0.778 ± 0.026	0.339TyrGln: 0.339 ± 0.021	1.349TyrArg: 1.349 ± 0.038	0.743TyrSer: 0.743 ± 0.029	0.857TyrThr: 0.857 ± 0.033	1.573TyrVal: 1.573 ± 0.039	0.225TyrTrp: 0.225 ± 0.013	0.318TyrTyr: 0.318 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3389 proteins (1148759 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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