Amino acid dipepetide frequency for Mucilaginibacter sp. HMF7856

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.478AlaAla: 7.478 ± 0.106	0.664AlaCys: 0.664 ± 0.022	5.259AlaAsp: 5.259 ± 0.06	4.576AlaGlu: 4.576 ± 0.076	3.604AlaPhe: 3.604 ± 0.054	6.294AlaGly: 6.294 ± 0.086	1.384AlaHis: 1.384 ± 0.038	5.759AlaIle: 5.759 ± 0.076	5.102AlaLys: 5.102 ± 0.073	7.301AlaLeu: 7.301 ± 0.081	1.882AlaMet: 1.882 ± 0.043	4.306AlaAsn: 4.306 ± 0.076	2.601AlaPro: 2.601 ± 0.062	3.239AlaGln: 3.239 ± 0.058	2.767AlaArg: 2.767 ± 0.059	4.588AlaSer: 4.588 ± 0.082	4.864AlaThr: 4.864 ± 0.136	5.45AlaVal: 5.45 ± 0.082	0.778AlaTrp: 0.778 ± 0.024	2.963AlaTyr: 2.963 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.584CysAla: 0.584 ± 0.024	0.125CysCys: 0.125 ± 0.012	0.422CysAsp: 0.422 ± 0.02	0.345CysGlu: 0.345 ± 0.016	0.417CysPhe: 0.417 ± 0.019	0.647CysGly: 0.647 ± 0.026	0.197CysHis: 0.197 ± 0.015	0.591CysIle: 0.591 ± 0.025	0.461CysLys: 0.461 ± 0.019	0.769CysLeu: 0.769 ± 0.03	0.169CysMet: 0.169 ± 0.013	0.379CysAsn: 0.379 ± 0.02	0.32CysPro: 0.32 ± 0.017	0.21CysGln: 0.21 ± 0.012	0.322CysArg: 0.322 ± 0.018	0.52CysSer: 0.52 ± 0.024	0.44CysThr: 0.44 ± 0.02	0.493CysVal: 0.493 ± 0.022	0.097CysTrp: 0.097 ± 0.009	0.286CysTyr: 0.286 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	4.454AspAla: 4.454 ± 0.07	0.414AspCys: 0.414 ± 0.023	2.924AspAsp: 2.924 ± 0.065	3.351AspGlu: 3.351 ± 0.061	2.976AspPhe: 2.976 ± 0.055	3.97AspGly: 3.97 ± 0.078	1.124AspHis: 1.124 ± 0.032	4.15AspIle: 4.15 ± 0.06	3.971AspLys: 3.971 ± 0.063	5.095AspLeu: 5.095 ± 0.073	1.267AspMet: 1.267 ± 0.034	3.013AspAsn: 3.013 ± 0.052	2.205AspPro: 2.205 ± 0.05	1.877AspGln: 1.877 ± 0.04	2.2AspArg: 2.2 ± 0.042	2.867AspSer: 2.867 ± 0.06	2.905AspThr: 2.905 ± 0.06	3.781AspVal: 3.781 ± 0.06	0.744AspTrp: 0.744 ± 0.028	2.553AspTyr: 2.553 ± 0.052	0.0AspXaa: 0.0 ± 0.0
Glu	4.126GluAla: 4.126 ± 0.078	0.296GluCys: 0.296 ± 0.018	2.648GluAsp: 2.648 ± 0.055	3.275GluGlu: 3.275 ± 0.076	2.238GluPhe: 2.238 ± 0.048	3.093GluGly: 3.093 ± 0.053	1.166GluHis: 1.166 ± 0.035	3.911GluIle: 3.911 ± 0.072	3.999GluLys: 3.999 ± 0.067	5.171GluLeu: 5.171 ± 0.091	1.441GluMet: 1.441 ± 0.04	2.947GluAsn: 2.947 ± 0.056	1.681GluPro: 1.681 ± 0.041	2.327GluGln: 2.327 ± 0.048	2.489GluArg: 2.489 ± 0.056	2.612GluSer: 2.612 ± 0.048	2.803GluThr: 2.803 ± 0.053	3.65GluVal: 3.65 ± 0.069	0.58GluTrp: 0.58 ± 0.025	1.828GluTyr: 1.828 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	3.727PheAla: 3.727 ± 0.063	0.457PheCys: 0.457 ± 0.019	3.051PheAsp: 3.051 ± 0.059	2.539PheGlu: 2.539 ± 0.051	2.313PhePhe: 2.313 ± 0.055	3.531PheGly: 3.531 ± 0.064	0.796PheHis: 0.796 ± 0.023	3.468PheIle: 3.468 ± 0.07	3.384PheLys: 3.384 ± 0.052	4.196PheLeu: 4.196 ± 0.077	1.004PheMet: 1.004 ± 0.029	2.872PheAsn: 2.872 ± 0.055	1.627PhePro: 1.627 ± 0.035	1.221PheGln: 1.221 ± 0.032	1.693PheArg: 1.693 ± 0.038	3.253PheSer: 3.253 ± 0.057	3.341PheThr: 3.341 ± 0.087	2.821PheVal: 2.821 ± 0.051	0.56PheTrp: 0.56 ± 0.023	2.131PheTyr: 2.131 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	5.058GlyAla: 5.058 ± 0.081	0.629GlyCys: 0.629 ± 0.029	3.596GlyAsp: 3.596 ± 0.06	3.176GlyGlu: 3.176 ± 0.055	3.576GlyPhe: 3.576 ± 0.068	5.288GlyGly: 5.288 ± 0.109	1.348GlyHis: 1.348 ± 0.035	5.181GlyIle: 5.181 ± 0.093	5.168GlyLys: 5.168 ± 0.068	6.284GlyLeu: 6.284 ± 0.09	1.786GlyMet: 1.786 ± 0.042	3.712GlyAsn: 3.712 ± 0.07	1.664GlyPro: 1.664 ± 0.041	2.344GlyGln: 2.344 ± 0.043	2.749GlyArg: 2.749 ± 0.044	4.474GlySer: 4.474 ± 0.074	4.549GlyThr: 4.549 ± 0.137	4.683GlyVal: 4.683 ± 0.065	0.901GlyTrp: 0.901 ± 0.029	3.236GlyTyr: 3.236 ± 0.062	0.0GlyXaa: 0.0 ± 0.0
His	1.273HisAla: 1.273 ± 0.035	0.197HisCys: 0.197 ± 0.014	1.0HisAsp: 1.0 ± 0.027	1.066HisGlu: 1.066 ± 0.035	1.114HisPhe: 1.114 ± 0.033	1.229HisGly: 1.229 ± 0.033	0.565HisHis: 0.565 ± 0.024	1.463HisIle: 1.463 ± 0.037	1.073HisLys: 1.073 ± 0.034	1.939HisLeu: 1.939 ± 0.041	0.393HisMet: 0.393 ± 0.017	1.03HisAsn: 1.03 ± 0.031	1.024HisPro: 1.024 ± 0.031	0.753HisGln: 0.753 ± 0.03	0.823HisArg: 0.823 ± 0.027	1.096HisSer: 1.096 ± 0.031	1.106HisThr: 1.106 ± 0.029	1.148HisVal: 1.148 ± 0.033	0.237HisTrp: 0.237 ± 0.014	0.893HisTyr: 0.893 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.202IleAla: 6.202 ± 0.086	0.721IleCys: 0.721 ± 0.026	4.312IleAsp: 4.312 ± 0.068	3.809IleGlu: 3.809 ± 0.067	3.136IlePhe: 3.136 ± 0.057	4.88IleGly: 4.88 ± 0.081	1.22IleHis: 1.22 ± 0.033	5.066IleIle: 5.066 ± 0.083	4.783IleLys: 4.783 ± 0.073	5.878IleLeu: 5.878 ± 0.088	1.369IleMet: 1.369 ± 0.04	4.274IleAsn: 4.274 ± 0.07	2.933IlePro: 2.933 ± 0.053	2.081IleGln: 2.081 ± 0.042	2.796IleArg: 2.796 ± 0.053	4.972IleSer: 4.972 ± 0.072	4.917IleThr: 4.917 ± 0.074	4.322IleVal: 4.322 ± 0.061	0.657IleTrp: 0.657 ± 0.023	2.658IleTyr: 2.658 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	5.322LysAla: 5.322 ± 0.076	0.325LysCys: 0.325 ± 0.016	3.903LysAsp: 3.903 ± 0.057	3.93LysGlu: 3.93 ± 0.07	2.665LysPhe: 2.665 ± 0.052	4.242LysGly: 4.242 ± 0.071	1.354LysHis: 1.354 ± 0.032	4.605LysIle: 4.605 ± 0.07	4.778LysLys: 4.778 ± 0.075	6.265LysLeu: 6.265 ± 0.075	1.795LysMet: 1.795 ± 0.043	3.74LysAsn: 3.74 ± 0.062	2.923LysPro: 2.923 ± 0.052	2.874LysGln: 2.874 ± 0.065	2.839LysArg: 2.839 ± 0.053	3.773LysSer: 3.773 ± 0.061	4.071LysThr: 4.071 ± 0.058	4.393LysVal: 4.393 ± 0.068	0.714LysTrp: 0.714 ± 0.024	2.769LysTyr: 2.769 ± 0.057	0.0LysXaa: 0.0 ± 0.0
Leu	7.248LeuAla: 7.248 ± 0.089	0.789LeuCys: 0.789 ± 0.029	4.801LeuAsp: 4.801 ± 0.07	4.517LeuGlu: 4.517 ± 0.086	4.402LeuPhe: 4.402 ± 0.068	5.747LeuGly: 5.747 ± 0.078	1.782LeuHis: 1.782 ± 0.04	6.484LeuIle: 6.484 ± 0.1	7.013LeuLys: 7.013 ± 0.097	8.944LeuLeu: 8.944 ± 0.117	2.172LeuMet: 2.172 ± 0.046	5.474LeuAsn: 5.474 ± 0.073	4.008LeuPro: 4.008 ± 0.062	3.754LeuGln: 3.754 ± 0.073	3.787LeuArg: 3.787 ± 0.057	6.614LeuSer: 6.614 ± 0.084	5.725LeuThr: 5.725 ± 0.078	5.506LeuVal: 5.506 ± 0.076	0.831LeuTrp: 0.831 ± 0.028	3.229LeuTyr: 3.229 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	1.951MetAla: 1.951 ± 0.047	0.145MetCys: 0.145 ± 0.013	1.19MetAsp: 1.19 ± 0.03	1.182MetGlu: 1.182 ± 0.039	0.883MetPhe: 0.883 ± 0.032	1.512MetGly: 1.512 ± 0.039	0.482MetHis: 0.482 ± 0.023	1.405MetIle: 1.405 ± 0.035	1.786MetLys: 1.786 ± 0.042	2.331MetLeu: 2.331 ± 0.059	0.638MetMet: 0.638 ± 0.028	1.155MetAsn: 1.155 ± 0.03	1.159MetPro: 1.159 ± 0.032	1.004MetGln: 1.004 ± 0.032	1.061MetArg: 1.061 ± 0.034	1.444MetSer: 1.444 ± 0.034	1.164MetThr: 1.164 ± 0.035	1.536MetVal: 1.536 ± 0.039	0.167MetTrp: 0.167 ± 0.012	0.668MetTyr: 0.668 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	4.442AsnAla: 4.442 ± 0.077	0.421AsnCys: 0.421 ± 0.02	2.877AsnAsp: 2.877 ± 0.067	2.706AsnGlu: 2.706 ± 0.053	2.756AsnPhe: 2.756 ± 0.057	4.191AsnGly: 4.191 ± 0.081	1.026AsnHis: 1.026 ± 0.029	4.142AsnIle: 4.142 ± 0.068	3.439AsnLys: 3.439 ± 0.062	5.009AsnLeu: 5.009 ± 0.059	1.164AsnMet: 1.164 ± 0.033	3.332AsnAsn: 3.332 ± 0.074	2.572AsnPro: 2.572 ± 0.054	2.033AsnGln: 2.033 ± 0.042	2.292AsnArg: 2.292 ± 0.046	3.218AsnSer: 3.218 ± 0.058	3.398AsnThr: 3.398 ± 0.089	3.457AsnVal: 3.457 ± 0.07	0.695AsnTrp: 0.695 ± 0.032	2.642AsnTyr: 2.642 ± 0.054	0.0AsnXaa: 0.0 ± 0.0
Pro	3.596ProAla: 3.596 ± 0.073	0.223ProCys: 0.223 ± 0.015	2.547ProAsp: 2.547 ± 0.051	2.515ProGlu: 2.515 ± 0.048	1.867ProPhe: 1.867 ± 0.039	2.933ProGly: 2.933 ± 0.054	0.768ProHis: 0.768 ± 0.027	2.371ProIle: 2.371 ± 0.056	2.263ProLys: 2.263 ± 0.043	3.203ProLeu: 3.203 ± 0.049	0.812ProMet: 0.812 ± 0.029	2.025ProAsn: 2.025 ± 0.047	1.187ProPro: 1.187 ± 0.045	1.645ProGln: 1.645 ± 0.04	1.175ProArg: 1.175 ± 0.033	2.233ProSer: 2.233 ± 0.046	2.089ProThr: 2.089 ± 0.076	3.392ProVal: 3.392 ± 0.059	0.387ProTrp: 0.387 ± 0.017	1.56ProTyr: 1.56 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	2.824GlnAla: 2.824 ± 0.054	0.166GlnCys: 0.166 ± 0.013	1.743GlnAsp: 1.743 ± 0.042	1.766GlnGlu: 1.766 ± 0.043	1.71GlnPhe: 1.71 ± 0.041	2.001GlnGly: 2.001 ± 0.045	0.82GlnHis: 0.82 ± 0.031	2.622GlnIle: 2.622 ± 0.052	2.665GlnLys: 2.665 ± 0.055	3.964GlnLeu: 3.964 ± 0.064	0.959GlnMet: 0.959 ± 0.031	2.039GlnAsn: 2.039 ± 0.041	1.623GlnPro: 1.623 ± 0.038	2.282GlnGln: 2.282 ± 0.086	1.713GlnArg: 1.713 ± 0.047	2.194GlnSer: 2.194 ± 0.05	2.219GlnThr: 2.219 ± 0.051	2.473GlnVal: 2.473 ± 0.051	0.443GlnTrp: 0.443 ± 0.023	1.525GlnTyr: 1.525 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	2.634ArgAla: 2.634 ± 0.056	0.233ArgCys: 0.233 ± 0.013	2.271ArgAsp: 2.271 ± 0.043	2.268ArgGlu: 2.268 ± 0.049	2.183ArgPhe: 2.183 ± 0.046	2.322ArgGly: 2.322 ± 0.049	0.808ArgHis: 0.808 ± 0.026	2.947ArgIle: 2.947 ± 0.048	2.723ArgLys: 2.723 ± 0.056	3.983ArgLeu: 3.983 ± 0.063	1.125ArgMet: 1.125 ± 0.034	2.202ArgAsn: 2.202 ± 0.044	1.448ArgPro: 1.448 ± 0.034	1.568ArgGln: 1.568 ± 0.034	1.781ArgArg: 1.781 ± 0.035	2.393ArgSer: 2.393 ± 0.047	2.07ArgThr: 2.07 ± 0.045	2.596ArgVal: 2.596 ± 0.047	0.453ArgTrp: 0.453 ± 0.02	1.846ArgTyr: 1.846 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	5.317SerAla: 5.317 ± 0.086	0.521SerCys: 0.521 ± 0.021	3.151SerAsp: 3.151 ± 0.056	2.764SerGlu: 2.764 ± 0.054	3.402SerPhe: 3.402 ± 0.056	4.925SerGly: 4.925 ± 0.088	1.159SerHis: 1.159 ± 0.033	4.212SerIle: 4.212 ± 0.067	3.707SerLys: 3.707 ± 0.064	5.739SerLeu: 5.739 ± 0.076	1.2SerMet: 1.2 ± 0.029	3.053SerAsn: 3.053 ± 0.068	2.414SerPro: 2.414 ± 0.052	2.142SerGln: 2.142 ± 0.043	2.441SerArg: 2.441 ± 0.046	3.844SerSer: 3.844 ± 0.069	3.746SerThr: 3.746 ± 0.08	4.372SerVal: 4.372 ± 0.059	0.662SerTrp: 0.662 ± 0.022	2.566SerTyr: 2.566 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	5.539ThrAla: 5.539 ± 0.118	0.4ThrCys: 0.4 ± 0.021	3.562ThrAsp: 3.562 ± 0.066	2.901ThrGlu: 2.901 ± 0.049	2.959ThrPhe: 2.959 ± 0.074	5.124ThrGly: 5.124 ± 0.12	1.055ThrHis: 1.055 ± 0.027	4.326ThrIle: 4.326 ± 0.079	3.115ThrLys: 3.115 ± 0.053	5.567ThrLeu: 5.567 ± 0.086	1.065ThrMet: 1.065 ± 0.027	3.111ThrAsn: 3.111 ± 0.096	2.918ThrPro: 2.918 ± 0.119	2.006ThrGln: 2.006 ± 0.041	2.094ThrArg: 2.094 ± 0.046	3.733ThrSer: 3.733 ± 0.084	4.03ThrThr: 4.03 ± 0.139	4.532ThrVal: 4.532 ± 0.114	0.625ThrTrp: 0.625 ± 0.024	2.535ThrTyr: 2.535 ± 0.086	0.0ThrXaa: 0.0 ± 0.0
Val	5.408ValAla: 5.408 ± 0.086	0.615ValCys: 0.615 ± 0.028	3.686ValAsp: 3.686 ± 0.054	3.224ValGlu: 3.224 ± 0.057	3.014ValPhe: 3.014 ± 0.058	4.007ValGly: 4.007 ± 0.06	1.168ValHis: 1.168 ± 0.033	5.021ValIle: 5.021 ± 0.077	4.626ValLys: 4.626 ± 0.065	6.032ValLeu: 6.032 ± 0.076	1.608ValMet: 1.608 ± 0.039	3.92ValAsn: 3.92 ± 0.062	2.449ValPro: 2.449 ± 0.043	2.133ValGln: 2.133 ± 0.042	2.439ValArg: 2.439 ± 0.046	4.434ValSer: 4.434 ± 0.067	4.581ValThr: 4.581 ± 0.114	4.513ValVal: 4.513 ± 0.082	0.615ValTrp: 0.615 ± 0.027	2.608ValTyr: 2.608 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.725TrpAla: 0.725 ± 0.027	0.097TrpCys: 0.097 ± 0.009	0.594TrpAsp: 0.594 ± 0.025	0.501TrpGlu: 0.501 ± 0.023	0.579TrpPhe: 0.579 ± 0.025	0.714TrpGly: 0.714 ± 0.027	0.29TrpHis: 0.29 ± 0.015	0.716TrpIle: 0.716 ± 0.029	0.624TrpLys: 0.624 ± 0.025	1.184TrpLeu: 1.184 ± 0.036	0.316TrpMet: 0.316 ± 0.015	0.621TrpAsn: 0.621 ± 0.023	0.327TrpPro: 0.327 ± 0.018	0.553TrpGln: 0.553 ± 0.024	0.5TrpArg: 0.5 ± 0.017	0.581TrpSer: 0.581 ± 0.023	0.557TrpThr: 0.557 ± 0.025	0.663TrpVal: 0.663 ± 0.025	0.19TrpTrp: 0.19 ± 0.012	0.464TrpTyr: 0.464 ± 0.03	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.953TyrAla: 2.953 ± 0.059	0.359TyrCys: 0.359 ± 0.02	2.302TyrAsp: 2.302 ± 0.046	1.872TyrGlu: 1.872 ± 0.041	2.191TyrPhe: 2.191 ± 0.05	2.75TyrGly: 2.75 ± 0.055	0.91TyrHis: 0.91 ± 0.032	2.547TyrIle: 2.547 ± 0.052	2.598TyrLys: 2.598 ± 0.052	3.973TyrLeu: 3.973 ± 0.063	0.695TyrMet: 0.695 ± 0.022	2.606TyrAsn: 2.606 ± 0.053	1.705TyrPro: 1.705 ± 0.04	1.705TyrGln: 1.705 ± 0.041	1.895TyrArg: 1.895 ± 0.046	2.497TyrSer: 2.497 ± 0.046	2.624TyrThr: 2.624 ± 0.091	2.321TyrVal: 2.321 ± 0.049	0.46TyrTrp: 0.46 ± 0.019	1.869TyrTyr: 1.869 ± 0.05	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3558 proteins (1173901 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---