Acinetobacter phage Aristophanes

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Beijerinckvirinae; unclassified Beijerinckvirinae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9VYN0|A0A7G9VYN0_9CAUD Recombination endonuclease VII OS=Acinetobacter phage Aristophanes OX=2759203 GN=Aristophanes_00021 PE=4 SV=1
MM1 pKa = 6.85STEE4 pKa = 4.35EE5 pKa = 4.34RR6 pKa = 11.84ITWDD10 pKa = 3.13EE11 pKa = 5.57LEE13 pKa = 4.28INWYY17 pKa = 10.15GSDD20 pKa = 3.7YY21 pKa = 11.35LL22 pKa = 4.41

Molecular weight: 2.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9VYM7|A0A7G9VYM7_9CAUD Uncharacterized protein OS=Acinetobacter phage Aristophanes OX=2759203 GN=Aristophanes_00018 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 9.14RR3 pKa = 11.84TLLWKK8 pKa = 9.52RR9 pKa = 11.84ARR11 pKa = 11.84RR12 pKa = 11.84GSQLIRR18 pKa = 11.84VYY20 pKa = 11.4ADD22 pKa = 3.23LTRR25 pKa = 11.84PPFSTYY31 pKa = 9.34RR32 pKa = 11.84VYY34 pKa = 10.18RR35 pKa = 11.84TLNIHH40 pKa = 6.01KK41 pKa = 9.0HH42 pKa = 4.88TII44 pKa = 3.3

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

13481

22

1394

293.1

32.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.137 ± 0.421

0.994 ± 0.167

5.957 ± 0.211

6.179 ± 0.329

3.538 ± 0.172

6.913 ± 0.258

1.995 ± 0.159

5.133 ± 0.196

5.897 ± 0.296

8.545 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.185

4.985 ± 0.348

3.761 ± 0.261

5.014 ± 0.403

4.97 ± 0.239

5.875 ± 0.306

6.557 ± 0.413

7.299 ± 0.279

1.335 ± 0.1

4.555 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski