Streptococcus intermedius B196

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus anginosus group; Streptococcus intermedius

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1812 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1ZF46|T1ZF46_STRIT DNA helicase OS=Streptococcus intermedius B196 OX=862967 GN=SIR_0979 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.47KK3 pKa = 9.84NQTYY7 pKa = 10.18DD8 pKa = 3.22LKK10 pKa = 11.32DD11 pKa = 2.91IMEE14 pKa = 4.4AVKK17 pKa = 10.69SEE19 pKa = 4.0EE20 pKa = 4.56LDD22 pKa = 4.85DD23 pKa = 5.86DD24 pKa = 3.94FCLYY28 pKa = 10.88AKK30 pKa = 10.6EE31 pKa = 3.9NGEE34 pKa = 4.84LNFQTSYY41 pKa = 10.5WLADD45 pKa = 3.56YY46 pKa = 9.19PQVVDD51 pKa = 4.62NKK53 pKa = 10.62DD54 pKa = 3.21VYY56 pKa = 10.01PRR58 pKa = 11.84QVRR61 pKa = 11.84EE62 pKa = 3.88QEE64 pKa = 4.18LEE66 pKa = 4.1LIYY69 pKa = 10.85YY70 pKa = 10.45GEE72 pKa = 4.79DD73 pKa = 3.33FADD76 pKa = 3.37VLLPVMEE83 pKa = 4.64QKK85 pKa = 11.18AEE87 pKa = 4.14VTDD90 pKa = 4.31EE91 pKa = 4.3EE92 pKa = 4.78CLQALLYY99 pKa = 10.58YY100 pKa = 10.12YY101 pKa = 10.46EE102 pKa = 6.27HH103 pKa = 7.93DD104 pKa = 3.82DD105 pKa = 4.85FMDD108 pKa = 4.88FEE110 pKa = 4.59

Molecular weight: 13.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1ZCP7|T1ZCP7_STRIT Magnesium and cobalt transporter OS=Streptococcus intermedius B196 OX=862967 GN=corA1 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.18IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 8.26HH16 pKa = 4.88GFRR19 pKa = 11.84HH20 pKa = 6.53RR21 pKa = 11.84MATKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.8GRR39 pKa = 11.84KK40 pKa = 8.76VLAAA44 pKa = 4.31

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1812

0

1812

573309

18

3042

316.4

35.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.201 ± 0.084

0.579 ± 0.018

5.373 ± 0.043

6.971 ± 0.067

4.675 ± 0.048

6.388 ± 0.053

1.949 ± 0.024

7.587 ± 0.056

7.303 ± 0.065

10.02 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.474 ± 0.03

4.828 ± 0.046

3.128 ± 0.03

4.04 ± 0.036

4.05 ± 0.047

6.112 ± 0.046

5.705 ± 0.055

6.803 ± 0.051

0.896 ± 0.017

3.919 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski