Dethiosulfatarculus sandiegensis
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5388 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D2GBJ9|A0A0D2GBJ9_9DELT Uncharacterized protein OS=Dethiosulfatarculus sandiegensis OX=1429043 GN=X474_19730 PE=4 SV=1
MM1 pKa = 7.8 PIEE4 pKa = 4.63 FDD6 pKa = 4.35 PNTSTYY12 pKa = 9.68 YY13 pKa = 8.23 QTSYY17 pKa = 8.85 TQNSYY22 pKa = 10.97 AQAPVEE28 pKa = 4.47 NPPPTPTGPQDD39 pKa = 3.24 PAQAVSAEE47 pKa = 4.14 KK48 pKa = 10.44 QIPQDD53 pKa = 3.49 PALVEE58 pKa = 4.2 PQDD61 pKa = 3.48 RR62 pKa = 11.84 PRR64 pKa = 11.84 PSGQEE69 pKa = 3.63 TSLTGSIVDD78 pKa = 3.7 TVVV81 pKa = 2.4
Molecular weight: 8.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.923
IPC_protein 3.77
Toseland 3.592
ProMoST 3.948
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.516
Solomon 3.719
Lehninger 3.681
Nozaki 3.91
DTASelect 4.05
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|A0A0D2G7A7|A0A0D2G7A7_9DELT Cytochrome C OS=Dethiosulfatarculus sandiegensis OX=1429043 GN=X474_26750 PE=4 SV=1
MM1 pKa = 7.69 RR2 pKa = 11.84 RR3 pKa = 11.84 LSKK6 pKa = 10.66 KK7 pKa = 9.78 GAQKK11 pKa = 11.18 ALLVSQKK18 pKa = 10.21 HH19 pKa = 5.43 HH20 pKa = 6.3 LQIQGRR26 pKa = 11.84 FAFALVVRR34 pKa = 5.09
Molecular weight: 3.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 10.774
IPC_protein 12.325
Toseland 12.501
ProMoST 12.998
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.34
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.106
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.04
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5388
0
5388
1659360
29
2205
308.0
34.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.122 ± 0.036
1.239 ± 0.014
5.062 ± 0.026
6.372 ± 0.037
4.126 ± 0.022
7.944 ± 0.034
1.922 ± 0.014
5.398 ± 0.031
6.176 ± 0.035
11.221 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.787 ± 0.015
3.444 ± 0.024
4.808 ± 0.025
3.709 ± 0.02
5.421 ± 0.03
5.632 ± 0.023
4.77 ± 0.022
6.917 ± 0.028
1.236 ± 0.014
2.693 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here