Menangle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Rubulavirinae; Pararubulavirus; Menangle pararubulavirus

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q91MK2|Q91MK2_9MONO Isoform of Q91MK1 Non-structural protein V OS=Menangle virus OX=152219 GN=V/P PE=3 SV=1
MM1 pKa = 7.4SSVFRR6 pKa = 11.84AFEE9 pKa = 4.3LFTLEE14 pKa = 4.59QEE16 pKa = 4.52QNEE19 pKa = 4.39HH20 pKa = 6.46GNDD23 pKa = 3.11IEE25 pKa = 5.19LPPEE29 pKa = 3.96TLRR32 pKa = 11.84TNIKK36 pKa = 10.08VCTLNNQEE44 pKa = 3.8PQARR48 pKa = 11.84HH49 pKa = 6.55DD50 pKa = 3.8MMCFCLRR57 pKa = 11.84LIASNSARR65 pKa = 11.84AAHH68 pKa = 5.96KK69 pKa = 9.34TGAILTLLSLPTAMMQNHH87 pKa = 6.33LRR89 pKa = 11.84IADD92 pKa = 4.31RR93 pKa = 11.84SPDD96 pKa = 3.38ADD98 pKa = 3.45IEE100 pKa = 4.11RR101 pKa = 11.84LEE103 pKa = 4.42IDD105 pKa = 3.46GFEE108 pKa = 4.53PGTFRR113 pKa = 11.84LRR115 pKa = 11.84ANARR119 pKa = 11.84TPMTNGEE126 pKa = 4.12VTALNLMAQDD136 pKa = 5.46LPDD139 pKa = 3.9TYY141 pKa = 11.76SNDD144 pKa = 3.75TPFLNPNTEE153 pKa = 4.16TEE155 pKa = 4.06QCDD158 pKa = 3.82EE159 pKa = 4.3MEE161 pKa = 4.17QFLNAIYY168 pKa = 10.51SVLVQVWVTVCKK180 pKa = 10.65CMTAHH185 pKa = 6.77DD186 pKa = 4.33QPTGSDD192 pKa = 3.06EE193 pKa = 4.13RR194 pKa = 11.84RR195 pKa = 11.84LAKK198 pKa = 10.17YY199 pKa = 8.02QQQGRR204 pKa = 11.84LDD206 pKa = 3.26QRR208 pKa = 11.84YY209 pKa = 8.96ALQPEE214 pKa = 4.98LRR216 pKa = 11.84RR217 pKa = 11.84QIQTCIRR224 pKa = 11.84SSLTIRR230 pKa = 11.84QFLTHH235 pKa = 6.73EE236 pKa = 4.26LQTARR241 pKa = 11.84KK242 pKa = 8.65QGAITGKK249 pKa = 9.08YY250 pKa = 7.82YY251 pKa = 11.55AMVGDD256 pKa = 3.45IGKK259 pKa = 10.43YY260 pKa = 9.05IDD262 pKa = 3.75NAGMSAFFMTMRR274 pKa = 11.84FALGTKK280 pKa = 8.92WPPLALAAFSGEE292 pKa = 3.98LLKK295 pKa = 11.09LKK297 pKa = 10.68SLMQLYY303 pKa = 10.2RR304 pKa = 11.84SLGDD308 pKa = 3.15RR309 pKa = 11.84ARR311 pKa = 11.84YY312 pKa = 7.24MALLEE317 pKa = 4.08MSEE320 pKa = 4.07MMEE323 pKa = 4.3FAPANYY329 pKa = 8.35PLCYY333 pKa = 9.75SYY335 pKa = 11.89AMGIGSVQDD344 pKa = 3.3PMMRR348 pKa = 11.84NYY350 pKa = 9.81TFARR354 pKa = 11.84PFLNPAYY361 pKa = 9.55FQLGVEE367 pKa = 4.29TANRR371 pKa = 11.84QQGSVDD377 pKa = 2.93KK378 pKa = 11.47AMAAEE383 pKa = 4.62LGLTEE388 pKa = 4.82DD389 pKa = 4.34EE390 pKa = 4.57KK391 pKa = 11.47RR392 pKa = 11.84DD393 pKa = 3.28MSAAVTRR400 pKa = 11.84LTTGRR405 pKa = 11.84GGNQAQEE412 pKa = 4.86LINVMGARR420 pKa = 11.84QGRR423 pKa = 11.84DD424 pKa = 2.79QGRR427 pKa = 11.84RR428 pKa = 11.84GNRR431 pKa = 11.84DD432 pKa = 2.9YY433 pKa = 11.69DD434 pKa = 3.7VVEE437 pKa = 4.34EE438 pKa = 4.18NEE440 pKa = 4.23EE441 pKa = 4.29TEE443 pKa = 4.12SDD445 pKa = 3.49SDD447 pKa = 3.61NDD449 pKa = 3.68EE450 pKa = 3.96EE451 pKa = 5.56QEE453 pKa = 4.07IQNRR457 pKa = 11.84PLPPIPQMPQNIDD470 pKa = 3.02WEE472 pKa = 4.5VRR474 pKa = 11.84LAEE477 pKa = 3.97IEE479 pKa = 4.2RR480 pKa = 11.84RR481 pKa = 11.84NQQMAARR488 pKa = 11.84DD489 pKa = 3.64RR490 pKa = 11.84PQAVVTADD498 pKa = 3.4VHH500 pKa = 6.63QEE502 pKa = 3.71PADD505 pKa = 3.74ARR507 pKa = 11.84VDD509 pKa = 3.88EE510 pKa = 4.44QDD512 pKa = 3.34MLLDD516 pKa = 4.26LDD518 pKa = 3.95MM519 pKa = 6.44

Molecular weight:
58.91 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q91MK1|Q91MK1_9MONO Phosphoprotein OS=Menangle virus OX=152219 GN=V/P PE=1 SV=1
MM1 pKa = 8.01ALRR4 pKa = 11.84QATIPVDD11 pKa = 3.38VDD13 pKa = 3.34KK14 pKa = 11.42DD15 pKa = 3.94SEE17 pKa = 4.5KK18 pKa = 11.38NNLNPFPIIPVTRR31 pKa = 11.84DD32 pKa = 3.25DD33 pKa = 4.13GSSTGRR39 pKa = 11.84LVKK42 pKa = 9.77QLRR45 pKa = 11.84VKK47 pKa = 10.76NLTPKK52 pKa = 10.79GSTEE56 pKa = 4.02LPLSFVNTYY65 pKa = 10.91GFIKK69 pKa = 10.5PLLTYY74 pKa = 10.35SEE76 pKa = 5.41FYY78 pKa = 11.11SEE80 pKa = 4.24VHH82 pKa = 6.02HH83 pKa = 6.42QASTPCLTACMIPFGAGPYY102 pKa = 9.53IEE104 pKa = 4.61NPHH107 pKa = 6.93RR108 pKa = 11.84ILDD111 pKa = 3.79EE112 pKa = 4.25CDD114 pKa = 2.99KK115 pKa = 11.49VNIIVRR121 pKa = 11.84KK122 pKa = 8.64SASLKK127 pKa = 9.96EE128 pKa = 4.15EE129 pKa = 3.52IVFDD133 pKa = 4.05VRR135 pKa = 11.84RR136 pKa = 11.84LPPIFNKK143 pKa = 10.17HH144 pKa = 5.34QISGNRR150 pKa = 11.84LICVPSEE157 pKa = 4.41KK158 pKa = 10.2YY159 pKa = 10.28VKK161 pKa = 10.72SPGKK165 pKa = 9.29MVAGTDD171 pKa = 3.49YY172 pKa = 10.63AYY174 pKa = 10.84QIAFVSLTFCPEE186 pKa = 3.79SQKK189 pKa = 10.37FRR191 pKa = 11.84VARR194 pKa = 11.84PLQTIRR200 pKa = 11.84SPIMRR205 pKa = 11.84SVQLEE210 pKa = 3.89VMLRR214 pKa = 11.84IDD216 pKa = 4.14CAADD220 pKa = 3.58SPLKK224 pKa = 10.51RR225 pKa = 11.84LLIVNPGSTDD235 pKa = 3.22YY236 pKa = 11.12YY237 pKa = 9.65ASVWFHH243 pKa = 6.09ICNLYY248 pKa = 10.45RR249 pKa = 11.84GNKK252 pKa = 8.18PFKK255 pKa = 10.75SYY257 pKa = 11.47DD258 pKa = 3.28DD259 pKa = 4.02TYY261 pKa = 11.34FSQKK265 pKa = 8.99CRR267 pKa = 11.84AMQLEE272 pKa = 4.42CGIVDD277 pKa = 3.09MWGPTLVVKK286 pKa = 10.71AHH288 pKa = 5.64GKK290 pKa = 7.5IPKK293 pKa = 7.84MAKK296 pKa = 9.7PFFSAKK302 pKa = 9.05GWACHH307 pKa = 5.86AFSDD311 pKa = 5.38AAPTLAKK318 pKa = 10.59ALWSVGAKK326 pKa = 7.82ITQVNAILQPSEE338 pKa = 4.15LSQMVQVSDD347 pKa = 4.24VIWPKK352 pKa = 10.72VKK354 pKa = 10.44LDD356 pKa = 4.06NRR358 pKa = 11.84IQDD361 pKa = 3.41YY362 pKa = 10.98SAAKK366 pKa = 8.94WNPFKK371 pKa = 11.11KK372 pKa = 10.26PP373 pKa = 3.15

Molecular weight:
41.96 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

1

7

4925

227

2269

703.6

78.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.97 ± 1.005

2.091 ± 0.251

5.36 ± 0.498

4.975 ± 0.572

3.655 ± 0.361

5.137 ± 0.601

1.949 ± 0.316

7.919 ± 0.734

5.015 ± 0.663

10.234 ± 1.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.843 ± 0.346

5.239 ± 0.47

5.178 ± 0.614

4.629 ± 0.673

4.954 ± 0.486

8.365 ± 0.634

6.477 ± 0.3

5.827 ± 0.613

1.056 ± 0.176

3.127 ± 0.415

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski