Menangle virus
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q91MK2|Q91MK2_9MONO Isoform of Q91MK1 Non-structural protein V OS=Menangle virus OX=152219 GN=V/P PE=3 SV=1
MM1 pKa = 7.4 SSVFRR6 pKa = 11.84 AFEE9 pKa = 4.3 LFTLEE14 pKa = 4.59 QEE16 pKa = 4.52 QNEE19 pKa = 4.39 HH20 pKa = 6.46 GNDD23 pKa = 3.11 IEE25 pKa = 5.19 LPPEE29 pKa = 3.96 TLRR32 pKa = 11.84 TNIKK36 pKa = 10.08 VCTLNNQEE44 pKa = 3.8 PQARR48 pKa = 11.84 HH49 pKa = 6.55 DD50 pKa = 3.8 MMCFCLRR57 pKa = 11.84 LIASNSARR65 pKa = 11.84 AAHH68 pKa = 5.96 KK69 pKa = 9.34 TGAILTLLSLPTAMMQNHH87 pKa = 6.33 LRR89 pKa = 11.84 IADD92 pKa = 4.31 RR93 pKa = 11.84 SPDD96 pKa = 3.38 ADD98 pKa = 3.45 IEE100 pKa = 4.11 RR101 pKa = 11.84 LEE103 pKa = 4.42 IDD105 pKa = 3.46 GFEE108 pKa = 4.53 PGTFRR113 pKa = 11.84 LRR115 pKa = 11.84 ANARR119 pKa = 11.84 TPMTNGEE126 pKa = 4.12 VTALNLMAQDD136 pKa = 5.46 LPDD139 pKa = 3.9 TYY141 pKa = 11.76 SNDD144 pKa = 3.75 TPFLNPNTEE153 pKa = 4.16 TEE155 pKa = 4.06 QCDD158 pKa = 3.82 EE159 pKa = 4.3 MEE161 pKa = 4.17 QFLNAIYY168 pKa = 10.51 SVLVQVWVTVCKK180 pKa = 10.65 CMTAHH185 pKa = 6.77 DD186 pKa = 4.33 QPTGSDD192 pKa = 3.06 EE193 pKa = 4.13 RR194 pKa = 11.84 RR195 pKa = 11.84 LAKK198 pKa = 10.17 YY199 pKa = 8.02 QQQGRR204 pKa = 11.84 LDD206 pKa = 3.26 QRR208 pKa = 11.84 YY209 pKa = 8.96 ALQPEE214 pKa = 4.98 LRR216 pKa = 11.84 RR217 pKa = 11.84 QIQTCIRR224 pKa = 11.84 SSLTIRR230 pKa = 11.84 QFLTHH235 pKa = 6.73 EE236 pKa = 4.26 LQTARR241 pKa = 11.84 KK242 pKa = 8.65 QGAITGKK249 pKa = 9.08 YY250 pKa = 7.82 YY251 pKa = 11.55 AMVGDD256 pKa = 3.45 IGKK259 pKa = 10.43 YY260 pKa = 9.05 IDD262 pKa = 3.75 NAGMSAFFMTMRR274 pKa = 11.84 FALGTKK280 pKa = 8.92 WPPLALAAFSGEE292 pKa = 3.98 LLKK295 pKa = 11.09 LKK297 pKa = 10.68 SLMQLYY303 pKa = 10.2 RR304 pKa = 11.84 SLGDD308 pKa = 3.15 RR309 pKa = 11.84 ARR311 pKa = 11.84 YY312 pKa = 7.24 MALLEE317 pKa = 4.08 MSEE320 pKa = 4.07 MMEE323 pKa = 4.3 FAPANYY329 pKa = 8.35 PLCYY333 pKa = 9.75 SYY335 pKa = 11.89 AMGIGSVQDD344 pKa = 3.3 PMMRR348 pKa = 11.84 NYY350 pKa = 9.81 TFARR354 pKa = 11.84 PFLNPAYY361 pKa = 9.55 FQLGVEE367 pKa = 4.29 TANRR371 pKa = 11.84 QQGSVDD377 pKa = 2.93 KK378 pKa = 11.47 AMAAEE383 pKa = 4.62 LGLTEE388 pKa = 4.82 DD389 pKa = 4.34 EE390 pKa = 4.57 KK391 pKa = 11.47 RR392 pKa = 11.84 DD393 pKa = 3.28 MSAAVTRR400 pKa = 11.84 LTTGRR405 pKa = 11.84 GGNQAQEE412 pKa = 4.86 LINVMGARR420 pKa = 11.84 QGRR423 pKa = 11.84 DD424 pKa = 2.79 QGRR427 pKa = 11.84 RR428 pKa = 11.84 GNRR431 pKa = 11.84 DD432 pKa = 2.9 YY433 pKa = 11.69 DD434 pKa = 3.7 VVEE437 pKa = 4.34 EE438 pKa = 4.18 NEE440 pKa = 4.23 EE441 pKa = 4.29 TEE443 pKa = 4.12 SDD445 pKa = 3.49 SDD447 pKa = 3.61 NDD449 pKa = 3.68 EE450 pKa = 3.96 EE451 pKa = 5.56 QEE453 pKa = 4.07 IQNRR457 pKa = 11.84 PLPPIPQMPQNIDD470 pKa = 3.02 WEE472 pKa = 4.5 VRR474 pKa = 11.84 LAEE477 pKa = 3.97 IEE479 pKa = 4.2 RR480 pKa = 11.84 RR481 pKa = 11.84 NQQMAARR488 pKa = 11.84 DD489 pKa = 3.64 RR490 pKa = 11.84 PQAVVTADD498 pKa = 3.4 VHH500 pKa = 6.63 QEE502 pKa = 3.71 PADD505 pKa = 3.74 ARR507 pKa = 11.84 VDD509 pKa = 3.88 EE510 pKa = 4.44 QDD512 pKa = 3.34 MLLDD516 pKa = 4.26 LDD518 pKa = 3.95 MM519 pKa = 6.44
Molecular weight: 58.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.896
IPC2_protein 4.902
IPC_protein 4.851
Toseland 4.724
ProMoST 4.952
Dawson 4.8
Bjellqvist 4.94
Wikipedia 4.66
Rodwell 4.711
Grimsley 4.635
Solomon 4.8
Lehninger 4.749
Nozaki 4.914
DTASelect 5.067
Thurlkill 4.711
EMBOSS 4.685
Sillero 4.978
Patrickios 4.075
IPC_peptide 4.813
IPC2_peptide 4.978
IPC2.peptide.svr19 4.955
Protein with the highest isoelectric point:
>tr|Q91MK1|Q91MK1_9MONO Phosphoprotein OS=Menangle virus OX=152219 GN=V/P PE=1 SV=1
MM1 pKa = 8.01 ALRR4 pKa = 11.84 QATIPVDD11 pKa = 3.38 VDD13 pKa = 3.34 KK14 pKa = 11.42 DD15 pKa = 3.94 SEE17 pKa = 4.5 KK18 pKa = 11.38 NNLNPFPIIPVTRR31 pKa = 11.84 DD32 pKa = 3.25 DD33 pKa = 4.13 GSSTGRR39 pKa = 11.84 LVKK42 pKa = 9.77 QLRR45 pKa = 11.84 VKK47 pKa = 10.76 NLTPKK52 pKa = 10.79 GSTEE56 pKa = 4.02 LPLSFVNTYY65 pKa = 10.91 GFIKK69 pKa = 10.5 PLLTYY74 pKa = 10.35 SEE76 pKa = 5.41 FYY78 pKa = 11.11 SEE80 pKa = 4.24 VHH82 pKa = 6.02 HH83 pKa = 6.42 QASTPCLTACMIPFGAGPYY102 pKa = 9.53 IEE104 pKa = 4.61 NPHH107 pKa = 6.93 RR108 pKa = 11.84 ILDD111 pKa = 3.79 EE112 pKa = 4.25 CDD114 pKa = 2.99 KK115 pKa = 11.49 VNIIVRR121 pKa = 11.84 KK122 pKa = 8.64 SASLKK127 pKa = 9.96 EE128 pKa = 4.15 EE129 pKa = 3.52 IVFDD133 pKa = 4.05 VRR135 pKa = 11.84 RR136 pKa = 11.84 LPPIFNKK143 pKa = 10.17 HH144 pKa = 5.34 QISGNRR150 pKa = 11.84 LICVPSEE157 pKa = 4.41 KK158 pKa = 10.2 YY159 pKa = 10.28 VKK161 pKa = 10.72 SPGKK165 pKa = 9.29 MVAGTDD171 pKa = 3.49 YY172 pKa = 10.63 AYY174 pKa = 10.84 QIAFVSLTFCPEE186 pKa = 3.79 SQKK189 pKa = 10.37 FRR191 pKa = 11.84 VARR194 pKa = 11.84 PLQTIRR200 pKa = 11.84 SPIMRR205 pKa = 11.84 SVQLEE210 pKa = 3.89 VMLRR214 pKa = 11.84 IDD216 pKa = 4.14 CAADD220 pKa = 3.58 SPLKK224 pKa = 10.51 RR225 pKa = 11.84 LLIVNPGSTDD235 pKa = 3.22 YY236 pKa = 11.12 YY237 pKa = 9.65 ASVWFHH243 pKa = 6.09 ICNLYY248 pKa = 10.45 RR249 pKa = 11.84 GNKK252 pKa = 8.18 PFKK255 pKa = 10.75 SYY257 pKa = 11.47 DD258 pKa = 3.28 DD259 pKa = 4.02 TYY261 pKa = 11.34 FSQKK265 pKa = 8.99 CRR267 pKa = 11.84 AMQLEE272 pKa = 4.42 CGIVDD277 pKa = 3.09 MWGPTLVVKK286 pKa = 10.71 AHH288 pKa = 5.64 GKK290 pKa = 7.5 IPKK293 pKa = 7.84 MAKK296 pKa = 9.7 PFFSAKK302 pKa = 9.05 GWACHH307 pKa = 5.86 AFSDD311 pKa = 5.38 AAPTLAKK318 pKa = 10.59 ALWSVGAKK326 pKa = 7.82 ITQVNAILQPSEE338 pKa = 4.15 LSQMVQVSDD347 pKa = 4.24 VIWPKK352 pKa = 10.72 VKK354 pKa = 10.44 LDD356 pKa = 4.06 NRR358 pKa = 11.84 IQDD361 pKa = 3.41 YY362 pKa = 10.98 SAAKK366 pKa = 8.94 WNPFKK371 pKa = 11.11 KK372 pKa = 10.26 PP373 pKa = 3.15
Molecular weight: 41.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.78
IPC2_protein 8.653
IPC_protein 8.507
Toseland 9.209
ProMoST 9.063
Dawson 9.516
Bjellqvist 9.355
Wikipedia 9.663
Rodwell 9.78
Grimsley 9.545
Solomon 9.56
Lehninger 9.531
Nozaki 9.545
DTASelect 9.253
Thurlkill 9.399
EMBOSS 9.677
Sillero 9.56
Patrickios 4.774
IPC_peptide 9.56
IPC2_peptide 8.112
IPC2.peptide.svr19 7.799
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
1
7
4925
227
2269
703.6
78.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.97 ± 1.005
2.091 ± 0.251
5.36 ± 0.498
4.975 ± 0.572
3.655 ± 0.361
5.137 ± 0.601
1.949 ± 0.316
7.919 ± 0.734
5.015 ± 0.663
10.234 ± 1.191
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.843 ± 0.346
5.239 ± 0.47
5.178 ± 0.614
4.629 ± 0.673
4.954 ± 0.486
8.365 ± 0.634
6.477 ± 0.3
5.827 ± 0.613
1.056 ± 0.176
3.127 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here