Mosquito circovirus
Average proteome isoelectric point is 8.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5C3Z3|A0A0C5C3Z3_9CIRC ATP-dependent helicase Rep OS=Mosquito circovirus OX=1611039 PE=3 SV=1
MM1 pKa = 7.59 SLIQFVCWTLNNYY14 pKa = 9.81 SEE16 pKa = 6.04 DD17 pKa = 4.05 EE18 pKa = 4.24 YY19 pKa = 11.55 QSLIEE24 pKa = 4.13 FSEE27 pKa = 3.47 WRR29 pKa = 11.84 YY30 pKa = 9.9 IVIGRR35 pKa = 11.84 EE36 pKa = 3.93 VGEE39 pKa = 4.37 TGTPHH44 pKa = 5.54 LQGYY48 pKa = 9.51 GEE50 pKa = 4.06 LKK52 pKa = 10.06 KK53 pKa = 10.47 RR54 pKa = 11.84 KK55 pKa = 9.53 KK56 pKa = 9.92 FAALKK61 pKa = 9.41 NLFPRR66 pKa = 11.84 VHH68 pKa = 6.07 WEE70 pKa = 3.43 QRR72 pKa = 11.84 RR73 pKa = 11.84 ASRR76 pKa = 11.84 DD77 pKa = 3.0 AAANYY82 pKa = 8.24 CKK84 pKa = 10.39 KK85 pKa = 10.27 EE86 pKa = 3.75 GRR88 pKa = 11.84 FEE90 pKa = 4.17 EE91 pKa = 4.91 RR92 pKa = 11.84 GSLPKK97 pKa = 10.36 NASEE101 pKa = 4.34 KK102 pKa = 10.19 SSKK105 pKa = 9.99 EE106 pKa = 3.31 AITRR110 pKa = 11.84 VKK112 pKa = 10.33 SGQTMRR118 pKa = 11.84 AILDD122 pKa = 3.95 DD123 pKa = 4.43 PPNLSGIRR131 pKa = 11.84 MCQIWLSYY139 pKa = 11.01 NEE141 pKa = 4.09 PKK143 pKa = 10.55 RR144 pKa = 11.84 NFKK147 pKa = 11.01 SEE149 pKa = 3.97 VFWYY153 pKa = 10.23 YY154 pKa = 10.77 GASGTGKK161 pKa = 8.35 TKK163 pKa = 10.46 LASEE167 pKa = 4.24 QAGPDD172 pKa = 4.02 AYY174 pKa = 9.78 WHH176 pKa = 7.46 DD177 pKa = 3.62 GTKK180 pKa = 9.79 WFDD183 pKa = 3.32 GYY185 pKa = 10.9 DD186 pKa = 3.35 AHH188 pKa = 6.85 EE189 pKa = 4.73 HH190 pKa = 5.87 VLLDD194 pKa = 4.21 DD195 pKa = 3.82 YY196 pKa = 11.56 RR197 pKa = 11.84 GGNMKK202 pKa = 10.39 FNFLLKK208 pKa = 10.6 FLDD211 pKa = 4.19 RR212 pKa = 11.84 YY213 pKa = 9.48 PLRR216 pKa = 11.84 LEE218 pKa = 4.06 VKK220 pKa = 9.72 GGYY223 pKa = 9.0 RR224 pKa = 11.84 QLLAKK229 pKa = 9.57 KK230 pKa = 9.69 IWVTSIKK237 pKa = 10.06 HH238 pKa = 5.29 PKK240 pKa = 9.13 EE241 pKa = 3.73 IYY243 pKa = 10.21 SFSEE247 pKa = 3.53 MDD249 pKa = 3.65 EE250 pKa = 4.0 PTEE253 pKa = 3.76 QLLRR257 pKa = 11.84 RR258 pKa = 11.84 ITTIKK263 pKa = 10.33 KK264 pKa = 8.32 MCACSQCEE272 pKa = 3.58 RR273 pKa = 11.84 FYY275 pKa = 11.6 GNRR278 pKa = 11.84 FEE280 pKa = 4.31 VSRR283 pKa = 11.84 FAVCGNTSTNSDD295 pKa = 3.09 TAANEE300 pKa = 4.09 TT301 pKa = 4.0
Molecular weight: 34.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.362
IPC2_protein 8.317
IPC_protein 8.214
Toseland 8.609
ProMoST 8.712
Dawson 9.063
Bjellqvist 9.077
Wikipedia 9.165
Rodwell 9.18
Grimsley 8.887
Solomon 9.151
Lehninger 9.121
Nozaki 9.194
DTASelect 8.916
Thurlkill 9.004
EMBOSS 9.209
Sillero 9.224
Patrickios 4.507
IPC_peptide 9.136
IPC2_peptide 7.834
IPC2.peptide.svr19 7.726
Protein with the highest isoelectric point:
>tr|A0A0C5C3Z3|A0A0C5C3Z3_9CIRC ATP-dependent helicase Rep OS=Mosquito circovirus OX=1611039 PE=3 SV=1
MM1 pKa = 7.66 AYY3 pKa = 9.97 KK4 pKa = 10.63 RR5 pKa = 11.84 MRR7 pKa = 11.84 RR8 pKa = 11.84 SRR10 pKa = 11.84 KK11 pKa = 7.8 RR12 pKa = 11.84 APRR15 pKa = 11.84 KK16 pKa = 8.08 RR17 pKa = 11.84 SYY19 pKa = 10.28 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 FRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 FNTSITRR33 pKa = 11.84 TVLRR37 pKa = 11.84 KK38 pKa = 9.77 FFDD41 pKa = 3.68 TSFSTTDD48 pKa = 2.7 VGYY51 pKa = 11.0 NYY53 pKa = 10.66 LSDD56 pKa = 3.99 NPKK59 pKa = 8.91 GTPGWSYY66 pKa = 9.41 YY67 pKa = 10.89 ASSFHH72 pKa = 7.04 HH73 pKa = 5.72 YY74 pKa = 9.79 RR75 pKa = 11.84 VVGLALKK82 pKa = 8.63 FVPKK86 pKa = 9.77 ATTAPMEE93 pKa = 4.4 TGMVHH98 pKa = 6.26 YY99 pKa = 10.46 DD100 pKa = 2.93 SSLFFARR107 pKa = 11.84 DD108 pKa = 3.19 ISPVVWAEE116 pKa = 3.92 TGNNLINKK124 pKa = 8.7 ALSIPNMKK132 pKa = 9.85 QRR134 pKa = 11.84 TLTRR138 pKa = 11.84 SFSLYY143 pKa = 10.02 FPMRR147 pKa = 11.84 KK148 pKa = 9.34 SSPTTYY154 pKa = 10.1 QATAGGYY161 pKa = 10.52 LSTDD165 pKa = 3.62 DD166 pKa = 4.6 PKK168 pKa = 9.11 TTQLVHH174 pKa = 5.53 MVSEE178 pKa = 4.45 KK179 pKa = 10.22 MSFAIPGRR187 pKa = 11.84 FYY189 pKa = 11.05 LSLYY193 pKa = 10.23 IRR195 pKa = 11.84 FKK197 pKa = 10.3 NKK199 pKa = 9.75 RR200 pKa = 11.84 YY201 pKa = 6.03 TTTPVV206 pKa = 2.65
Molecular weight: 24.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.295
IPC2_protein 10.292
IPC_protein 11.082
Toseland 10.965
ProMoST 10.833
Dawson 11.067
Bjellqvist 10.847
Wikipedia 11.345
Rodwell 11.228
Grimsley 11.125
Solomon 11.228
Lehninger 11.184
Nozaki 10.935
DTASelect 10.847
Thurlkill 10.965
EMBOSS 11.374
Sillero 10.994
Patrickios 10.921
IPC_peptide 11.228
IPC2_peptide 9.867
IPC2.peptide.svr19 7.897
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
507
206
301
253.5
29.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.114 ± 0.177
1.381 ± 0.846
3.945 ± 0.335
5.523 ± 2.49
5.72 ± 0.659
5.917 ± 0.948
1.972 ± 0.019
3.748 ± 0.511
7.692 ± 0.549
7.101 ± 0.484
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.761 ± 0.687
4.339 ± 0.279
4.339 ± 0.91
2.564 ± 0.678
8.876 ± 1.402
8.481 ± 1.049
7.495 ± 1.95
4.339 ± 0.613
1.972 ± 0.613
5.72 ± 0.659
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here