Caenorhabditis nigoni
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44209 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2G5SJT8|A0A2G5SJT8_9PELO Isoform of A0A2G5SJE5 Uncharacterized protein OS=Caenorhabditis nigoni OX=1611254 GN=Cnig_chr_X.g22250 PE=4 SV=1
MM1 pKa = 7.1 TVASKK6 pKa = 11.2 LNDD9 pKa = 3.03 ASTFTGYY16 pKa = 9.78 PDD18 pKa = 3.19 SFGYY22 pKa = 10.68 AGGLNDD28 pKa = 3.5 HH29 pKa = 6.24 SQYY32 pKa = 10.91 PVYY35 pKa = 10.87 NYY37 pKa = 10.61 TEE39 pKa = 4.34 FDD41 pKa = 3.74 KK42 pKa = 11.01 MPTPADD48 pKa = 4.38 DD49 pKa = 5.05 LDD51 pKa = 6.18 DD52 pKa = 7.09 DD53 pKa = 4.25 IDD55 pKa = 5.65 LDD57 pKa = 4.34 LRR59 pKa = 11.84 YY60 pKa = 10.73 VNANLTQASWQPPAQDD76 pKa = 3.32 QTCLIFFSAAPEE88 pKa = 4.13 AEE90 pKa = 4.46 FEE92 pKa = 4.35 DD93 pKa = 4.42 TSIQTTYY100 pKa = 11.6 DD101 pKa = 3.03 SFKK104 pKa = 10.46 TVIGVLIGDD113 pKa = 3.85 ATSIPGITDD122 pKa = 3.23 SVNAKK127 pKa = 9.73 NLNDD131 pKa = 3.79 EE132 pKa = 4.34 EE133 pKa = 4.35 AQSVVQKK140 pKa = 11.09 LLDD143 pKa = 3.75 SLPP146 pKa = 3.71
Molecular weight: 15.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.605
IPC_protein 3.605
Toseland 3.376
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.605
Rodwell 3.439
Grimsley 3.287
Solomon 3.605
Lehninger 3.567
Nozaki 3.745
DTASelect 4.024
Thurlkill 3.452
EMBOSS 3.605
Sillero 3.732
Patrickios 0.896
IPC_peptide 3.592
IPC2_peptide 3.706
IPC2.peptide.svr19 3.707
Protein with the highest isoelectric point:
>tr|A0A2G5T830|A0A2G5T830_9PELO Uncharacterized protein OS=Caenorhabditis nigoni OX=1611254 GN=Cnig_chr_V.g17022 PE=4 SV=1
MM1 pKa = 6.82 EE2 pKa = 5.24 TSPSSEE8 pKa = 3.82 TTTISEE14 pKa = 4.42 SSSQSTIAPTSTTMEE29 pKa = 4.22 TSPSSEE35 pKa = 3.82 TTTISEE41 pKa = 4.42 SSSQSTIAPTSTTMEE56 pKa = 4.22 TSPSSEE62 pKa = 3.82 TTTISEE68 pKa = 4.42 SSSQSTIAPTSTTMEE83 pKa = 4.22 TSPSSEE89 pKa = 3.82 TTTISEE95 pKa = 4.42 SSSQSTIAPTSTTMEE110 pKa = 4.22 TSPSSEE116 pKa = 3.82 TTTISEE122 pKa = 4.42 SSSQSTIAPTSTTMEE137 pKa = 4.22 TSPSSEE143 pKa = 3.82 TTTISEE149 pKa = 4.42 SSSQSTIRR157 pKa = 11.84 RR158 pKa = 11.84 HH159 pKa = 4.65 PRR161 pKa = 11.84 PWKK164 pKa = 8.72 QARR167 pKa = 11.84 PLKK170 pKa = 10.42 LLQFLNRR177 pKa = 11.84 AANLRR182 pKa = 11.84 LRR184 pKa = 11.84 RR185 pKa = 11.84 HH186 pKa = 5.21 PRR188 pKa = 11.84 PWKK191 pKa = 8.72 QARR194 pKa = 11.84 PLKK197 pKa = 10.42 LLQFLNRR204 pKa = 11.84 AANLRR209 pKa = 11.84 LRR211 pKa = 11.84 RR212 pKa = 11.84 HH213 pKa = 5.21 PRR215 pKa = 11.84 PWKK218 pKa = 8.72 QARR221 pKa = 11.84 PLKK224 pKa = 10.42 LLQFLNRR231 pKa = 11.84 AANLRR236 pKa = 11.84 LRR238 pKa = 11.84 RR239 pKa = 11.84 HH240 pKa = 5.21 PRR242 pKa = 11.84 PWKK245 pKa = 8.72 QARR248 pKa = 11.84 PLKK251 pKa = 10.42 LLQFLNRR258 pKa = 11.84 AANLRR263 pKa = 11.84 LRR265 pKa = 11.84 RR266 pKa = 11.84 HH267 pKa = 5.21 PRR269 pKa = 11.84 PWKK272 pKa = 8.72 QARR275 pKa = 11.84 PLKK278 pKa = 10.42 LLQFLNRR285 pKa = 11.84 AANLRR290 pKa = 11.84 LRR292 pKa = 11.84 RR293 pKa = 11.84 HH294 pKa = 5.21 PRR296 pKa = 11.84 PWKK299 pKa = 8.72 QARR302 pKa = 11.84 PLKK305 pKa = 10.42 LLQFLNRR312 pKa = 11.84 AANLRR317 pKa = 11.84 LRR319 pKa = 11.84 RR320 pKa = 11.84 HH321 pKa = 5.21 PRR323 pKa = 11.84 PWKK326 pKa = 8.72 QARR329 pKa = 11.84 PLKK332 pKa = 10.42 LLQFLNRR339 pKa = 11.84 AANLRR344 pKa = 11.84 LRR346 pKa = 11.84 RR347 pKa = 11.84 HH348 pKa = 5.21 PRR350 pKa = 11.84 PWKK353 pKa = 8.72 QARR356 pKa = 11.84 PLKK359 pKa = 10.42 LLQFLNRR366 pKa = 11.84 AANLRR371 pKa = 11.84 LRR373 pKa = 11.84 RR374 pKa = 11.84 HH375 pKa = 5.21 PRR377 pKa = 11.84 PWKK380 pKa = 8.72 QARR383 pKa = 11.84 PLKK386 pKa = 10.42 LLQFLNRR393 pKa = 11.84 AANLRR398 pKa = 11.84 LRR400 pKa = 11.84 RR401 pKa = 11.84 HH402 pKa = 5.21 PRR404 pKa = 11.84 PWKK407 pKa = 8.72 QARR410 pKa = 11.84 PLKK413 pKa = 10.42 LLQFLNRR420 pKa = 11.84 AANLRR425 pKa = 11.84 LRR427 pKa = 11.84 RR428 pKa = 11.84 HH429 pKa = 5.21 PRR431 pKa = 11.84 PWKK434 pKa = 8.72 QARR437 pKa = 11.84 PLKK440 pKa = 10.42 LLQFLNRR447 pKa = 11.84 AANLQVRR454 pKa = 11.84 RR455 pKa = 11.84 HH456 pKa = 5.06 PRR458 pKa = 11.84 PWKK461 pKa = 8.72 QARR464 pKa = 11.84 PLKK467 pKa = 10.42 LLQFLNRR474 pKa = 11.84 AANLRR479 pKa = 11.84 LRR481 pKa = 11.84 RR482 pKa = 11.84 HH483 pKa = 5.21 PRR485 pKa = 11.84 PWKK488 pKa = 8.72 QARR491 pKa = 11.84 PLKK494 pKa = 10.42 LLQFLNRR501 pKa = 11.84 AANLRR506 pKa = 11.84 LRR508 pKa = 11.84 RR509 pKa = 11.84 HH510 pKa = 5.21 PRR512 pKa = 11.84 PWKK515 pKa = 8.72 QARR518 pKa = 11.84 PLKK521 pKa = 10.42 LLQFLNRR528 pKa = 11.84 AANLRR533 pKa = 11.84 LRR535 pKa = 11.84 RR536 pKa = 11.84 HH537 pKa = 5.21 PRR539 pKa = 11.84 PWKK542 pKa = 8.72 QARR545 pKa = 11.84 PLKK548 pKa = 10.42 LLQFLNRR555 pKa = 11.84 AANLRR560 pKa = 11.84 LRR562 pKa = 11.84 RR563 pKa = 11.84 HH564 pKa = 5.21 PRR566 pKa = 11.84 PWKK569 pKa = 8.72 QARR572 pKa = 11.84 PLKK575 pKa = 10.42 LLQFLNRR582 pKa = 11.84 AANLRR587 pKa = 11.84 LRR589 pKa = 11.84 RR590 pKa = 11.84 HH591 pKa = 5.21 PRR593 pKa = 11.84 PWKK596 pKa = 8.72 QARR599 pKa = 11.84 PLKK602 pKa = 10.42 LLQFLNRR609 pKa = 11.84 AANLRR614 pKa = 11.84 LRR616 pKa = 11.84 RR617 pKa = 11.84 HH618 pKa = 5.21 PRR620 pKa = 11.84 PWKK623 pKa = 8.72 QARR626 pKa = 11.84 PLKK629 pKa = 10.42 LLQFLNRR636 pKa = 11.84 AANLRR641 pKa = 11.84 LRR643 pKa = 11.84 RR644 pKa = 11.84 HH645 pKa = 5.21 PRR647 pKa = 11.84 PWKK650 pKa = 8.72 QARR653 pKa = 11.84 PLKK656 pKa = 10.42 LLQFLNRR663 pKa = 11.84 AANLRR668 pKa = 11.84 LRR670 pKa = 11.84 RR671 pKa = 11.84 HH672 pKa = 5.21 PRR674 pKa = 11.84 PWKK677 pKa = 8.72 QARR680 pKa = 11.84 PLKK683 pKa = 10.42 LLQFLNRR690 pKa = 11.84 AANLRR695 pKa = 11.84 LRR697 pKa = 11.84 RR698 pKa = 11.84 HH699 pKa = 5.21 PRR701 pKa = 11.84 PWKK704 pKa = 8.72 QARR707 pKa = 11.84 PLKK710 pKa = 10.42 LLQFLNRR717 pKa = 11.84 AANLRR722 pKa = 11.84 LRR724 pKa = 11.84 RR725 pKa = 11.84 HH726 pKa = 5.21 PRR728 pKa = 11.84 PWKK731 pKa = 8.72 QARR734 pKa = 11.84 PLKK737 pKa = 10.42 LLQFLNRR744 pKa = 11.84 AANLRR749 pKa = 11.84 LRR751 pKa = 11.84 RR752 pKa = 11.84 HH753 pKa = 5.21 PRR755 pKa = 11.84 PWKK758 pKa = 8.72 QARR761 pKa = 11.84 PLKK764 pKa = 10.42 LLQFLNRR771 pKa = 11.84 AANLRR776 pKa = 11.84 LRR778 pKa = 11.84 RR779 pKa = 11.84 HH780 pKa = 5.21 PRR782 pKa = 11.84 PWKK785 pKa = 8.72 QARR788 pKa = 11.84 PLKK791 pKa = 10.42 LLQFLNRR798 pKa = 11.84 AANLRR803 pKa = 11.84 LRR805 pKa = 11.84 RR806 pKa = 11.84 HH807 pKa = 5.21 PRR809 pKa = 11.84 PWKK812 pKa = 8.72 QARR815 pKa = 11.84 PLKK818 pKa = 10.42 LLQFLNRR825 pKa = 11.84 AANLRR830 pKa = 11.84 LRR832 pKa = 11.84 RR833 pKa = 11.84 HH834 pKa = 5.21 PRR836 pKa = 11.84 PWKK839 pKa = 8.72 QARR842 pKa = 11.84 PLKK845 pKa = 10.42 LLQFLNRR852 pKa = 11.84 AANLRR857 pKa = 11.84 LRR859 pKa = 11.84 RR860 pKa = 11.84 HH861 pKa = 5.21 PRR863 pKa = 11.84 PWKK866 pKa = 8.72 QARR869 pKa = 11.84 PLKK872 pKa = 10.42 LLQFLNRR879 pKa = 11.84 AANLRR884 pKa = 11.84 LRR886 pKa = 11.84 RR887 pKa = 11.84 HH888 pKa = 5.21 PRR890 pKa = 11.84 PWKK893 pKa = 8.72 QARR896 pKa = 11.84 PLKK899 pKa = 10.42 LLQFLNRR906 pKa = 11.84 AANLRR911 pKa = 11.84 LRR913 pKa = 11.84 RR914 pKa = 11.84 HH915 pKa = 5.21 PRR917 pKa = 11.84 PWKK920 pKa = 8.72 QARR923 pKa = 11.84 PLKK926 pKa = 10.42 LLQFLNRR933 pKa = 11.84 AANLRR938 pKa = 11.84 LRR940 pKa = 11.84 RR941 pKa = 11.84 HH942 pKa = 5.21 PRR944 pKa = 11.84 PWKK947 pKa = 8.72 QARR950 pKa = 11.84 PLKK953 pKa = 10.42 LLQFLNRR960 pKa = 11.84 AANLRR965 pKa = 11.84 LRR967 pKa = 11.84 RR968 pKa = 11.84 HH969 pKa = 5.21 PRR971 pKa = 11.84 PWKK974 pKa = 8.72 QARR977 pKa = 11.84 PLKK980 pKa = 10.42 LLQFLNRR987 pKa = 11.84 AANLRR992 pKa = 11.84 LRR994 pKa = 11.84 RR995 pKa = 11.84 HH996 pKa = 5.21 PRR998 pKa = 11.84 PWKK1001 pKa = 8.72 QARR1004 pKa = 11.84 PLKK1007 pKa = 10.42 LLQFLNRR1014 pKa = 11.84 AANLRR1019 pKa = 11.84 LRR1021 pKa = 11.84 RR1022 pKa = 11.84 HH1023 pKa = 5.21 PRR1025 pKa = 11.84 PWKK1028 pKa = 8.72 QARR1031 pKa = 11.84 PLKK1034 pKa = 10.42 LLQFLNRR1041 pKa = 11.84 AANLRR1046 pKa = 11.84 LRR1048 pKa = 11.84 RR1049 pKa = 11.84 HH1050 pKa = 5.21 PRR1052 pKa = 11.84 PWKK1055 pKa = 8.72 QARR1058 pKa = 11.84 PLKK1061 pKa = 10.42 LLQFLNRR1068 pKa = 11.84 AANLRR1073 pKa = 11.84 LRR1075 pKa = 11.84 RR1076 pKa = 11.84 HH1077 pKa = 5.21 PRR1079 pKa = 11.84 PWKK1082 pKa = 8.72 QARR1085 pKa = 11.84 PLKK1088 pKa = 10.42 LLQFLNRR1095 pKa = 11.84 AANLQVRR1102 pKa = 11.84 RR1103 pKa = 11.84 HH1104 pKa = 5.06 PRR1106 pKa = 11.84 PWKK1109 pKa = 8.72 QARR1112 pKa = 11.84 PLKK1115 pKa = 10.42 LLQFLNRR1122 pKa = 11.84 AANLRR1127 pKa = 11.84 LRR1129 pKa = 11.84 RR1130 pKa = 11.84 HH1131 pKa = 5.21 PRR1133 pKa = 11.84 PWKK1136 pKa = 8.72 QARR1139 pKa = 11.84 PLKK1142 pKa = 10.42 LLQFLNRR1149 pKa = 11.84 AANLRR1154 pKa = 11.84 LRR1156 pKa = 11.84 RR1157 pKa = 11.84 HH1158 pKa = 5.21 PRR1160 pKa = 11.84 PWKK1163 pKa = 8.72 QARR1166 pKa = 11.84 PLKK1169 pKa = 10.42 LLQFLNRR1176 pKa = 11.84 AANLRR1181 pKa = 11.84 LRR1183 pKa = 11.84 RR1184 pKa = 11.84 HH1185 pKa = 5.21 PRR1187 pKa = 11.84 PWKK1190 pKa = 8.72 QARR1193 pKa = 11.84 PLKK1196 pKa = 10.42 LLQFLNRR1203 pKa = 11.84 AANLRR1208 pKa = 11.84 LRR1210 pKa = 11.84 RR1211 pKa = 11.84 HH1212 pKa = 5.21 PRR1214 pKa = 11.84 PWKK1217 pKa = 8.72 QARR1220 pKa = 11.84 PLKK1223 pKa = 10.42 LLQFLNRR1230 pKa = 11.84 AANLRR1235 pKa = 11.84 FADD1238 pKa = 3.67 IHH1240 pKa = 6.3 DD1241 pKa = 4.68 HH1242 pKa = 6.01 GNKK1245 pKa = 9.48 PVLL1248 pKa = 3.75
Molecular weight: 150.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.524
IPC2_protein 11.257
IPC_protein 12.852
Toseland 13.013
ProMoST 13.51
Dawson 13.013
Bjellqvist 13.013
Wikipedia 13.495
Rodwell 12.647
Grimsley 13.056
Solomon 13.51
Lehninger 13.422
Nozaki 13.013
DTASelect 13.013
Thurlkill 13.013
EMBOSS 13.525
Sillero 13.013
Patrickios 12.354
IPC_peptide 13.525
IPC2_peptide 12.501
IPC2.peptide.svr19 9.199
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28846
15363
44209
17210255
66
16129
389.3
44.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.04 ± 0.018
1.933 ± 0.012
5.44 ± 0.012
7.109 ± 0.024
4.636 ± 0.013
5.23 ± 0.016
2.304 ± 0.007
5.998 ± 0.012
6.595 ± 0.02
8.452 ± 0.015
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.652 ± 0.007
4.87 ± 0.009
5.108 ± 0.02
4.126 ± 0.013
5.37 ± 0.013
7.993 ± 0.017
5.8 ± 0.019
6.075 ± 0.012
1.166 ± 0.005
3.101 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here