Aspergillus heteromorphus CBS 117.55
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10837 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A317V505|A0A317V505_9EURO Isochorismatase hydrolase OS=Aspergillus heteromorphus CBS 117.55 OX=1448321 GN=BO70DRAFT_366221 PE=3 SV=1
DDD2 pKa = 4.18 DD3 pKa = 4.43 GDDD6 pKa = 3.78 DD7 pKa = 4.0 GDDD10 pKa = 3.55 DD11 pKa = 3.93 GDDD14 pKa = 3.55 DD15 pKa = 3.93 GDDD18 pKa = 3.55 DD19 pKa = 3.93 GDDD22 pKa = 3.55 DD23 pKa = 3.93 GDDD26 pKa = 3.55 DD27 pKa = 3.93 GDDD30 pKa = 3.55 DD31 pKa = 3.93 GDDD34 pKa = 3.55 DD35 pKa = 3.93 GDDD38 pKa = 3.55 DD39 pKa = 3.93 GDDD42 pKa = 3.58 DD43 pKa = 3.94 GDDD46 pKa = 3.71 DD47 pKa = 3.99 DDD49 pKa = 5.25 DDD51 pKa = 4.06 GDDD54 pKa = 3.61 YY55 pKa = 9.91 DDD57 pKa = 4.85 DDD59 pKa = 4.02 GDDD62 pKa = 4.0 DD63 pKa = 3.67 VASSTYYY70 pKa = 8.64 TPATSSSAAVSDDD83 pKa = 5.65 DD84 pKa = 3.87 EE85 pKa = 5.13 CSPQCTEEE93 pKa = 5.21 ISNDDD98 pKa = 3.34 TDDD101 pKa = 3.66 SSASSKKK108 pKa = 9.61 SNWFDDD114 pKa = 3.21 GLSKKK119 pKa = 10.21 TSDDD123 pKa = 3.51 YY124 pKa = 9.44 TWMLTQMRR132 pKa = 11.84 TGPLLTKKK140 pKa = 9.97 DD141 pKa = 3.47 SGMTTSEEE149 pKa = 3.85 VVFDDD154 pKa = 3.48 SAINFSQTNIRR165 pKa = 11.84 GCTVVIVVSKKK176 pKa = 9.06 YY177 pKa = 10.34 AYYY180 pKa = 9.52 AHHH183 pKa = 6.57 WEEE186 pKa = 5.06 DD187 pKa = 3.53 GFVSQTNDDD196 pKa = 2.38 TTAVLNEEE204 pKa = 4.31 EEE206 pKa = 4.28 QNTVLTYYY214 pKa = 8.94 TNDDD218 pKa = 3.64 NLKKK222 pKa = 9.16 PQLTGSTAYYY232 pKa = 9.25 DDD234 pKa = 4.44 GTQVYYY240 pKa = 10.22 GTPQAQSGKKK250 pKa = 8.33 TAKKK254 pKa = 10.75 YY255 pKa = 7.66 AQVSRR260 pKa = 11.84 VGITVNEEE268 pKa = 4.0 LGLSAAPTEEE278 pKa = 4.66 YY279 pKa = 10.7 YY280 pKa = 10.9 YY281 pKa = 10.27 TEEE284 pKa = 3.89 EE285 pKa = 3.81 AGEEE289 pKa = 4.36 KKK291 pKa = 9.32 LFQFPNSCDDD301 pKa = 3.43 KK302 pKa = 10.77 LWQIWYYY309 pKa = 10.23 DDD311 pKa = 4.22 DD312 pKa = 3.7 NSALWASSQNGACSSAPAPASASSSVTAVRR342 pKa = 11.84 TPPFPIFTDDD352 pKa = 3.49 STLIARR358 pKa = 11.84 PRR360 pKa = 11.84 TTTQAYYY367 pKa = 8.17
Molecular weight: 39.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.592
IPC_protein 3.643
Toseland 3.401
ProMoST 3.846
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.63
Rodwell 3.465
Grimsley 3.3
Solomon 3.656
Lehninger 3.617
Nozaki 3.77
DTASelect 4.088
Thurlkill 3.465
EMBOSS 3.643
Sillero 3.77
Patrickios 1.265
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|A0A317WLR7|A0A317WLR7_9EURO Uncharacterized protein OS=Aspergillus heteromorphus CBS 117.55 OX=1448321 GN=BO70DRAFT_193952 PE=4 SV=1
MM1 pKa = 7.09 FCFRR5 pKa = 11.84 SRR7 pKa = 11.84 ALPAAIRR14 pKa = 11.84 AYY16 pKa = 10.0 AAPTARR22 pKa = 11.84 LSSAPITPIATSLSTPLRR40 pKa = 11.84 TLSSTTLSSLRR51 pKa = 11.84 QHH53 pKa = 6.67 IPSQTLSSSSTTFSSLRR70 pKa = 11.84 PLLTTSPFTAQQTRR84 pKa = 11.84 SFSASASLGGKK95 pKa = 9.56 RR96 pKa = 11.84 NTYY99 pKa = 9.03 NPSRR103 pKa = 11.84 RR104 pKa = 11.84 VQKK107 pKa = 10.1 RR108 pKa = 11.84 RR109 pKa = 11.84 HH110 pKa = 5.44 GFLSRR115 pKa = 11.84 LRR117 pKa = 11.84 TRR119 pKa = 11.84 GGRR122 pKa = 11.84 NILMRR127 pKa = 11.84 RR128 pKa = 11.84 RR129 pKa = 11.84 QKK131 pKa = 10.54 GRR133 pKa = 11.84 KK134 pKa = 7.54 NLSWW138 pKa = 4.16
Molecular weight: 15.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.471
Toseland 12.632
ProMoST 13.13
Dawson 12.632
Bjellqvist 12.632
Wikipedia 13.115
Rodwell 12.223
Grimsley 12.676
Solomon 13.13
Lehninger 13.027
Nozaki 12.632
DTASelect 12.632
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 11.945
IPC_peptide 13.13
IPC2_peptide 12.12
IPC2.peptide.svr19 9.11
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10837
0
10837
4977815
49
9562
459.3
50.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.592 ± 0.023
1.281 ± 0.009
5.625 ± 0.016
6.009 ± 0.026
3.718 ± 0.013
6.915 ± 0.023
2.507 ± 0.011
4.785 ± 0.016
4.252 ± 0.022
9.142 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.162 ± 0.008
3.419 ± 0.013
6.333 ± 0.028
3.971 ± 0.016
6.3 ± 0.021
8.387 ± 0.028
6.064 ± 0.013
6.252 ± 0.016
1.474 ± 0.009
2.814 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here