Arthrobacter echini
Average proteome isoelectric point is 6.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2848 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4S5E3U7|A0A4S5E3U7_9MICC PH domain-containing protein OS=Arthrobacter echini OX=1529066 GN=E8P82_10560 PE=4 SV=1
MM1 pKa = 7.87 RR2 pKa = 11.84 KK3 pKa = 7.64 TRR5 pKa = 11.84 YY6 pKa = 6.62 TAAAIAAVTALTLSACGGGDD26 pKa = 3.62 DD27 pKa = 5.18 DD28 pKa = 5.46 AAGSSGASGEE38 pKa = 4.23 AGGEE42 pKa = 4.18 SVRR45 pKa = 11.84 IGIKK49 pKa = 10.03 FDD51 pKa = 3.5 QPGLGFDD58 pKa = 3.68 EE59 pKa = 5.09 GGSYY63 pKa = 10.6 SGFDD67 pKa = 3.18 VDD69 pKa = 3.87 VARR72 pKa = 11.84 YY73 pKa = 7.93 VANEE77 pKa = 3.86 LGYY80 pKa = 9.29 TDD82 pKa = 3.77 EE83 pKa = 5.1 QIDD86 pKa = 4.08 FVEE89 pKa = 4.63 SPSANRR95 pKa = 11.84 EE96 pKa = 3.59 NMLANDD102 pKa = 3.73 QVDD105 pKa = 4.42 MIFATYY111 pKa = 10.44 SITDD115 pKa = 3.53 TRR117 pKa = 11.84 KK118 pKa = 8.21 EE119 pKa = 4.34 TVDD122 pKa = 3.6 FAGPYY127 pKa = 9.96 FIAGQDD133 pKa = 3.64 LLVPTDD139 pKa = 3.6 SDD141 pKa = 3.41 ITGPEE146 pKa = 3.99 DD147 pKa = 3.57 LEE149 pKa = 4.25 GRR151 pKa = 11.84 NLCSVTGSTSAEE163 pKa = 4.12 QIKK166 pKa = 9.42 EE167 pKa = 3.96 QYY169 pKa = 10.1 PGVQLVEE176 pKa = 3.88 QPGYY180 pKa = 10.33 AEE182 pKa = 5.14 CVTAMGGGQIDD193 pKa = 4.3 AVTTDD198 pKa = 4.42 DD199 pKa = 5.44 IILAGLAAQDD209 pKa = 4.12 ANAGQYY215 pKa = 9.93 RR216 pKa = 11.84 VVGNTFSEE224 pKa = 4.05 EE225 pKa = 4.35 RR226 pKa = 11.84 YY227 pKa = 10.31 GVGLPPGSDD236 pKa = 2.74 RR237 pKa = 11.84 CEE239 pKa = 4.96 DD240 pKa = 3.02 INAAITQMIEE250 pKa = 3.49 SGAWEE255 pKa = 3.94 EE256 pKa = 3.88 ALAANTEE263 pKa = 4.32 GADD266 pKa = 3.42 YY267 pKa = 10.83 SYY269 pKa = 11.78 NEE271 pKa = 4.86 DD272 pKa = 4.09 LNPPEE277 pKa = 5.16 PEE279 pKa = 3.99 PCAA282 pKa = 3.95
Molecular weight: 29.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.706
IPC_protein 3.706
Toseland 3.49
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.605
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 4.012
Thurlkill 3.541
EMBOSS 3.617
Sillero 3.821
Patrickios 0.871
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>tr|A0A4S5E9K8|A0A4S5E9K8_9MICC HAD-IB family hydrolase OS=Arthrobacter echini OX=1529066 GN=E8P82_00285 PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2848
0
2848
924687
24
1983
324.7
34.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.485 ± 0.06
0.566 ± 0.011
6.185 ± 0.036
5.879 ± 0.039
3.069 ± 0.03
8.99 ± 0.047
2.067 ± 0.021
4.294 ± 0.033
2.006 ± 0.035
10.425 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.864 ± 0.018
2.069 ± 0.022
5.4 ± 0.032
3.059 ± 0.025
7.349 ± 0.048
6.114 ± 0.032
6.154 ± 0.036
8.659 ± 0.042
1.366 ± 0.018
1.999 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here