Frateuria aurantia (strain ATCC 33424 / DSM 6220 / KCTC 2777 / LMG 1558 / NBRC 3245 / NCIMB 13370) (Acetobacter aurantius)
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3098 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H8L640|H8L640_FRAAD Uncharacterized protein OS=Frateuria aurantia (strain ATCC 33424 / DSM 6220 / KCTC 2777 / LMG 1558 / NBRC 3245 / NCIMB 13370) OX=767434 GN=Fraau_1461 PE=4 SV=1
MM1 pKa = 7.24 KK2 pKa = 10.3 RR3 pKa = 11.84 VLTPEE8 pKa = 4.28 AEE10 pKa = 4.18 AEE12 pKa = 4.07 RR13 pKa = 11.84 ADD15 pKa = 4.06 FDD17 pKa = 5.21 SEE19 pKa = 4.28 FDD21 pKa = 3.98 GGNCSCHH28 pKa = 5.72 LTPPCGSCTHH38 pKa = 7.26 PGNPDD43 pKa = 2.92 NQAEE47 pKa = 4.31 DD48 pKa = 3.6 DD49 pKa = 4.31 SAWMEE54 pKa = 4.04 VDD56 pKa = 3.9 DD57 pKa = 6.43 DD58 pKa = 4.81 YY59 pKa = 12.17 DD60 pKa = 3.82 GVEE63 pKa = 4.05 EE64 pKa = 4.19
Molecular weight: 7.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.732
IPC_protein 3.668
Toseland 3.478
ProMoST 3.821
Dawson 3.668
Bjellqvist 3.897
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.389
Solomon 3.643
Lehninger 3.605
Nozaki 3.808
DTASelect 3.999
Thurlkill 3.541
EMBOSS 3.63
Sillero 3.795
Patrickios 0.769
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|H8L6X7|H8L6X7_FRAAD Uncharacterized protein OS=Frateuria aurantia (strain ATCC 33424 / DSM 6220 / KCTC 2777 / LMG 1558 / NBRC 3245 / NCIMB 13370) OX=767434 GN=Fraau_2539 PE=4 SV=1
MM1 pKa = 7.42 KK2 pKa = 9.47 PANFRR7 pKa = 11.84 RR8 pKa = 11.84 LINLWPPYY16 pKa = 9.45 LLNSIRR22 pKa = 11.84 VQSIARR28 pKa = 11.84 DD29 pKa = 3.3 WSEE32 pKa = 3.67 ARR34 pKa = 11.84 VVLHH38 pKa = 6.18 LRR40 pKa = 11.84 PWNRR44 pKa = 11.84 NIMRR48 pKa = 11.84 TQFGGNLFAMIDD60 pKa = 4.01 PMPMLLAMRR69 pKa = 11.84 QLGTHH74 pKa = 4.44 YY75 pKa = 10.65 HH76 pKa = 5.31 VWDD79 pKa = 3.89 KK80 pKa = 11.15 AAEE83 pKa = 4.06 IDD85 pKa = 4.27 FVAPGRR91 pKa = 11.84 SHH93 pKa = 5.1 VHH95 pKa = 4.42 ATIRR99 pKa = 11.84 MPACAMDD106 pKa = 5.12 EE107 pKa = 4.13 IRR109 pKa = 11.84 SAAQTGDD116 pKa = 3.16 KK117 pKa = 10.03 VLRR120 pKa = 11.84 WFDD123 pKa = 3.4 IDD125 pKa = 3.19 ICHH128 pKa = 7.42 ADD130 pKa = 3.87 GSPVAHH136 pKa = 6.23 VRR138 pKa = 11.84 KK139 pKa = 9.46 QLYY142 pKa = 9.48 VRR144 pKa = 11.84 LKK146 pKa = 8.79 SQHH149 pKa = 5.27 RR150 pKa = 3.66
Molecular weight: 17.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.531
IPC_protein 10.379
Toseland 10.555
ProMoST 10.54
Dawson 10.657
Bjellqvist 10.394
Wikipedia 10.877
Rodwell 10.804
Grimsley 10.716
Solomon 10.789
Lehninger 10.745
Nozaki 10.555
DTASelect 10.379
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.599
Patrickios 10.54
IPC_peptide 10.789
IPC2_peptide 9.487
IPC2.peptide.svr19 8.721
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3098
0
3098
1035801
29
2518
334.3
36.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.22 ± 0.062
0.907 ± 0.015
5.458 ± 0.03
4.94 ± 0.046
3.144 ± 0.026
8.464 ± 0.042
2.632 ± 0.02
4.528 ± 0.033
2.533 ± 0.031
11.461 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.345 ± 0.021
2.381 ± 0.029
5.41 ± 0.036
4.591 ± 0.035
7.369 ± 0.04
5.897 ± 0.048
4.711 ± 0.041
6.904 ± 0.031
1.65 ± 0.021
2.454 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here