Mageeibacillus indolicus (strain UPII9-5) (Clostridiales genomosp. BVAB3 (strain UPII9-5))

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Mageeibacillus; Mageeibacillus indolicus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1566 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3QZQ9|D3QZQ9_MAGIU Peptidase family M13 OS=Mageeibacillus indolicus (strain UPII9-5) OX=699246 GN=HMPREF0868_0060 PE=4 SV=1
MM1 pKa = 7.57LNLNNIFYY9 pKa = 10.53SDD11 pKa = 4.13EE12 pKa = 4.27CDD14 pKa = 3.58CGCGHH19 pKa = 6.01TKK21 pKa = 8.71EE22 pKa = 5.08HH23 pKa = 5.54EE24 pKa = 4.24HH25 pKa = 5.77EE26 pKa = 4.97HH27 pKa = 6.89DD28 pKa = 4.03LEE30 pKa = 6.1HH31 pKa = 6.57EE32 pKa = 4.38HH33 pKa = 7.15SCGCGCGCEE42 pKa = 4.22DD43 pKa = 3.84EE44 pKa = 5.66GEE46 pKa = 4.12IGEE49 pKa = 5.0SIVTMVDD56 pKa = 2.97EE57 pKa = 4.58EE58 pKa = 4.47TGEE61 pKa = 4.25EE62 pKa = 4.26YY63 pKa = 10.67QFSIVDD69 pKa = 3.63DD70 pKa = 4.23FDD72 pKa = 5.19FEE74 pKa = 4.96GEE76 pKa = 4.67VYY78 pKa = 10.47CVLLTVDD85 pKa = 4.83KK86 pKa = 11.02DD87 pKa = 3.63PEE89 pKa = 3.96ALIVKK94 pKa = 8.99VVTDD98 pKa = 4.39EE99 pKa = 4.19NDD101 pKa = 2.78NDD103 pKa = 3.78YY104 pKa = 11.3FMSLEE109 pKa = 4.13DD110 pKa = 3.56EE111 pKa = 4.56EE112 pKa = 4.84YY113 pKa = 11.13DD114 pKa = 3.61RR115 pKa = 11.84VSAEE119 pKa = 3.82YY120 pKa = 10.64EE121 pKa = 4.13RR122 pKa = 11.84ILAEE126 pKa = 5.78ADD128 pKa = 4.18DD129 pKa = 4.69EE130 pKa = 6.11DD131 pKa = 6.49DD132 pKa = 5.4DD133 pKa = 5.02GSEE136 pKa = 4.14YY137 pKa = 10.93VIEE140 pKa = 4.67GGKK143 pKa = 9.22EE144 pKa = 3.55

Molecular weight: 16.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3R277|D3R277_MAGIU Peptidase M20/M25/M40 family OS=Mageeibacillus indolicus (strain UPII9-5) OX=699246 GN=HMPREF0868_0981 PE=4 SV=1
MM1 pKa = 7.84LKK3 pKa = 10.4RR4 pKa = 11.84FFQPDD9 pKa = 3.02AYY11 pKa = 10.3FRR13 pKa = 11.84SLADD17 pKa = 2.99IDD19 pKa = 4.82FNSLYY24 pKa = 9.33EE25 pKa = 3.73QGYY28 pKa = 9.68RR29 pKa = 11.84LVLLDD34 pKa = 4.24MDD36 pKa = 3.88NTLIRR41 pKa = 11.84DD42 pKa = 3.64GHH44 pKa = 5.42RR45 pKa = 11.84EE46 pKa = 3.5RR47 pKa = 11.84TAFSDD52 pKa = 3.61LQINRR57 pKa = 11.84MLDD60 pKa = 3.08AGFKK64 pKa = 10.63CLILSNGPAEE74 pKa = 4.12RR75 pKa = 11.84VKK77 pKa = 10.85PFADD81 pKa = 3.43LHH83 pKa = 6.63HH84 pKa = 7.4LDD86 pKa = 5.99FIADD90 pKa = 3.48SGKK93 pKa = 8.2PSRR96 pKa = 11.84RR97 pKa = 11.84GVHH100 pKa = 6.19LACQKK105 pKa = 10.59FSLSPKK111 pKa = 7.94EE112 pKa = 3.84TLLIGDD118 pKa = 4.15QIFTDD123 pKa = 3.93VLCGRR128 pKa = 11.84RR129 pKa = 11.84SGVLTLLVAPLATGEE144 pKa = 3.74KK145 pKa = 8.62WYY147 pKa = 10.34IRR149 pKa = 11.84IKK151 pKa = 10.58RR152 pKa = 11.84IFEE155 pKa = 4.09LPFISSLKK163 pKa = 8.53MNKK166 pKa = 9.5GLPSSGRR173 pKa = 11.84KK174 pKa = 8.72IFANWLARR182 pKa = 11.84RR183 pKa = 11.84RR184 pKa = 11.84FKK186 pKa = 10.78NLRR189 pKa = 11.84RR190 pKa = 11.84FLPRR194 pKa = 11.84RR195 pKa = 11.84RR196 pKa = 11.84LRR198 pKa = 11.84TLPHH202 pKa = 6.68RR203 pKa = 11.84KK204 pKa = 9.78NIFSRR209 pKa = 11.84LLKK212 pKa = 9.7RR213 pKa = 11.84RR214 pKa = 11.84HH215 pKa = 4.32YY216 pKa = 11.01HH217 pKa = 6.07FFYY220 pKa = 10.64LKK222 pKa = 10.42KK223 pKa = 10.21PSDD226 pKa = 3.69YY227 pKa = 10.76HH228 pKa = 7.99ISNGG232 pKa = 3.49

Molecular weight: 27.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1566

0

1566

537427

37

2451

343.2

38.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.067 ± 0.07

1.323 ± 0.024

5.38 ± 0.056

6.169 ± 0.052

4.214 ± 0.041

6.927 ± 0.054

1.785 ± 0.024

6.878 ± 0.061

6.476 ± 0.072

10.088 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.502 ± 0.035

4.641 ± 0.052

4.079 ± 0.047

3.285 ± 0.03

4.781 ± 0.05

5.841 ± 0.046

5.426 ± 0.045

6.591 ± 0.049

0.935 ± 0.019

3.612 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski