Desulfotomaculum ruminis (strain ATCC 23193 / DSM 2154 / NCIMB 8452 / DL)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfotomaculum; Desulfotomaculum ruminis

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3763 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6DME8|F6DME8_DESRL Putative sigma factor OS=Desulfotomaculum ruminis (strain ATCC 23193 / DSM 2154 / NCIMB 8452 / DL) OX=696281 GN=Desru_3506 PE=4 SV=1
MM1 pKa = 7.62AKK3 pKa = 9.37MSNYY7 pKa = 10.21LEE9 pKa = 4.25NALINCTLRR18 pKa = 11.84GIPYY22 pKa = 7.73TPPSVIYY29 pKa = 10.02AALFISDD36 pKa = 4.3PTDD39 pKa = 3.58ADD41 pKa = 3.38TGTEE45 pKa = 4.11VSGGGYY51 pKa = 6.69TRR53 pKa = 11.84KK54 pKa = 9.39PVTFDD59 pKa = 3.25TPDD62 pKa = 2.92NGAAVNNADD71 pKa = 4.21LAFPVATGNWGTVTHH86 pKa = 6.8IGLYY90 pKa = 10.24DD91 pKa = 3.8AEE93 pKa = 4.53TDD95 pKa = 3.95GNLLFYY101 pKa = 11.2GDD103 pKa = 5.03LNTPRR108 pKa = 11.84AINIDD113 pKa = 3.4NQLIILEE120 pKa = 4.42GGLMVTLDD128 pKa = 3.39

Molecular weight: 13.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6DRM7|F6DRM7_DESRL Uncharacterized protein OS=Desulfotomaculum ruminis (strain ATCC 23193 / DSM 2154 / NCIMB 8452 / DL) OX=696281 GN=Desru_1523 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.31QPKK8 pKa = 9.71KK9 pKa = 8.96RR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 4.85QKK14 pKa = 9.0VHH16 pKa = 5.81GFLKK20 pKa = 10.67RR21 pKa = 11.84MATKK25 pKa = 10.41SGRR28 pKa = 11.84NVLKK32 pKa = 10.48RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.24GRR39 pKa = 11.84KK40 pKa = 8.9RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3763

0

3763

1098365

31

1736

291.9

32.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.144 ± 0.04

1.167 ± 0.016

4.696 ± 0.026

6.958 ± 0.038

3.926 ± 0.028

7.717 ± 0.04

1.848 ± 0.016

6.862 ± 0.039

6.052 ± 0.033

10.546 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.727 ± 0.019

3.845 ± 0.027

4.228 ± 0.026

4.015 ± 0.03

5.114 ± 0.033

5.457 ± 0.026

5.108 ± 0.03

7.384 ± 0.033

1.032 ± 0.014

3.177 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski