Thermosulfidibacter takaii (strain DSM 17441 / JCM 13301 / NBRC 103674 / ABI70S6)
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1756 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S3QSZ6|A0A0S3QSZ6_THET7 Chemotaxis protein MotA OS=Thermosulfidibacter takaii (strain DSM 17441 / JCM 13301 / NBRC 103674 / ABI70S6) OX=1298851 GN=motA PE=3 SV=1
MM1 pKa = 7.24 AQAGSLCDD9 pKa = 3.6 KK10 pKa = 10.67 VDD12 pKa = 3.45 YY13 pKa = 11.33 DD14 pKa = 3.31 IDD16 pKa = 3.97 NFVAYY21 pKa = 10.18 AGIDD25 pKa = 3.43 VQLFEE30 pKa = 4.7 NLNLFADD37 pKa = 4.73 VSYY40 pKa = 9.39 TWTKK44 pKa = 11.23 AEE46 pKa = 4.03 GDD48 pKa = 3.7 NPHH51 pKa = 7.07 FGNPWNVITDD61 pKa = 3.55 HH62 pKa = 6.93 GGTPEE67 pKa = 4.84 DD68 pKa = 3.82 PHH70 pKa = 7.78 PSYY73 pKa = 10.45 TGSYY77 pKa = 10.0 DD78 pKa = 2.87 PWFVITYY85 pKa = 8.53 TNDD88 pKa = 3.03 MDD90 pKa = 6.18 DD91 pKa = 3.44 MKK93 pKa = 11.12 DD94 pKa = 3.29 WYY96 pKa = 10.29 DD97 pKa = 3.49 LEE99 pKa = 4.65 YY100 pKa = 10.72 EE101 pKa = 4.03 IFEE104 pKa = 4.38 VSGGFQWDD112 pKa = 3.09 IFKK115 pKa = 10.84 NLSLKK120 pKa = 9.38 TVATYY125 pKa = 11.08 RR126 pKa = 11.84 NFDD129 pKa = 3.65 DD130 pKa = 4.36 KK131 pKa = 11.69 EE132 pKa = 4.06 EE133 pKa = 3.92 YY134 pKa = 10.39 LYY136 pKa = 11.05 EE137 pKa = 5.42 DD138 pKa = 4.32 NDD140 pKa = 4.0 GEE142 pKa = 4.32 MYY144 pKa = 10.42 IINTSLIWRR153 pKa = 11.84 FF154 pKa = 3.2
Molecular weight: 18.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.795
IPC_protein 3.795
Toseland 3.567
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.164
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.923
Patrickios 0.477
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.812
Protein with the highest isoelectric point:
>tr|A0A0S3QRP9|A0A0S3QRP9_THET7 Cytochrome c biogenesis protein OS=Thermosulfidibacter takaii (strain DSM 17441 / JCM 13301 / NBRC 103674 / ABI70S6) OX=1298851 GN=resB PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.28 QPSKK9 pKa = 10.25 LKK11 pKa = 9.55 MKK13 pKa = 9.95 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.93 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 EE29 pKa = 3.16 IIKK32 pKa = 9.81 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.68 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LTVV44 pKa = 3.11
Molecular weight: 5.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 10.979
IPC_protein 12.413
Toseland 12.574
ProMoST 13.071
Dawson 12.574
Bjellqvist 12.574
Wikipedia 13.042
Rodwell 12.281
Grimsley 12.618
Solomon 13.071
Lehninger 12.969
Nozaki 12.574
DTASelect 12.574
Thurlkill 12.574
EMBOSS 13.071
Sillero 12.574
Patrickios 12.003
IPC_peptide 13.071
IPC2_peptide 12.062
IPC2.peptide.svr19 9.07
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1756
0
1756
571920
37
1542
325.7
36.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.116 ± 0.058
1.235 ± 0.025
4.924 ± 0.043
8.0 ± 0.065
4.58 ± 0.05
7.075 ± 0.049
1.723 ± 0.027
7.228 ± 0.049
8.012 ± 0.055
9.995 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.272 ± 0.029
3.452 ± 0.04
4.182 ± 0.034
2.428 ± 0.029
5.185 ± 0.048
5.215 ± 0.048
4.455 ± 0.041
8.264 ± 0.058
1.058 ± 0.025
3.6 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here