Lake Sarah-associated circular virus-39

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQQ0|A0A140AQQ0_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-39 OX=1685767 PE=3 SV=1
MM1 pKa = 7.28YY2 pKa = 8.05MARR5 pKa = 11.84NRR7 pKa = 11.84NFVFTWNNYY16 pKa = 8.48TEE18 pKa = 5.43DD19 pKa = 3.44SEE21 pKa = 4.53QFLRR25 pKa = 11.84SRR27 pKa = 11.84VDD29 pKa = 3.14EE30 pKa = 4.27GVIKK34 pKa = 10.77YY35 pKa = 10.09VGYY38 pKa = 10.18CKK40 pKa = 10.31EE41 pKa = 4.04IAPSTGTPHH50 pKa = 6.75LQGFCSFEE58 pKa = 4.11NKK60 pKa = 10.01KK61 pKa = 10.37SVQQVRR67 pKa = 11.84VILVGCHH74 pKa = 5.07VEE76 pKa = 4.22TMLGSISQNEE86 pKa = 4.54DD87 pKa = 3.37YY88 pKa = 10.7CSKK91 pKa = 10.83AGEE94 pKa = 4.4LINYY98 pKa = 7.43GVQPVTNDD106 pKa = 2.79NKK108 pKa = 10.43GRR110 pKa = 11.84AEE112 pKa = 3.73QLRR115 pKa = 11.84WQRR118 pKa = 11.84ARR120 pKa = 11.84EE121 pKa = 4.03SAKK124 pKa = 10.49LGQFDD129 pKa = 6.3DD130 pKa = 3.52IDD132 pKa = 5.38ADD134 pKa = 3.61IFIRR138 pKa = 11.84CYY140 pKa = 9.79STLKK144 pKa = 10.54RR145 pKa = 11.84IRR147 pKa = 11.84TDD149 pKa = 3.33YY150 pKa = 8.97TAKK153 pKa = 10.5PPPEE157 pKa = 5.34DD158 pKa = 3.51VTCYY162 pKa = 9.98WIYY165 pKa = 11.16GPTGTGKK172 pKa = 9.3SHH174 pKa = 6.42SVEE177 pKa = 4.14TTFPLCYY184 pKa = 9.29KK185 pKa = 10.79KK186 pKa = 10.9NMDD189 pKa = 3.76DD190 pKa = 4.02PKK192 pKa = 10.73WFDD195 pKa = 3.36GYY197 pKa = 10.93QGEE200 pKa = 4.4EE201 pKa = 3.77TVYY204 pKa = 11.22LEE206 pKa = 6.39DD207 pKa = 3.45IDD209 pKa = 4.66KK210 pKa = 11.25YY211 pKa = 7.36QVKK214 pKa = 9.65WGGLLKK220 pKa = 10.55RR221 pKa = 11.84LADD224 pKa = 3.96RR225 pKa = 11.84WPLLVNTKK233 pKa = 10.51GSMQYY238 pKa = 10.04IRR240 pKa = 11.84PKK242 pKa = 10.43RR243 pKa = 11.84IIVTSNYY250 pKa = 10.12NLDD253 pKa = 4.74EE254 pKa = 4.28IWSDD258 pKa = 3.88SGTLDD263 pKa = 3.38PLLRR267 pKa = 11.84RR268 pKa = 11.84FTVILKK274 pKa = 9.63EE275 pKa = 4.15SQEE278 pKa = 4.0QVIDD282 pKa = 3.71FTT284 pKa = 4.84

Molecular weight: 32.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQQ0|A0A140AQQ0_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-39 OX=1685767 PE=3 SV=1
MM1 pKa = 6.9KK2 pKa = 10.2RR3 pKa = 11.84KK4 pKa = 9.37YY5 pKa = 9.95RR6 pKa = 11.84SSDD9 pKa = 3.07SYY11 pKa = 11.69LPSTLTNSSPYY22 pKa = 9.73VRR24 pKa = 11.84RR25 pKa = 11.84TKK27 pKa = 10.09RR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 6.49TRR32 pKa = 11.84RR33 pKa = 11.84VSIDD37 pKa = 3.02YY38 pKa = 10.27GRR40 pKa = 11.84RR41 pKa = 11.84SSTSSGGLTNYY52 pKa = 8.28TSSSGSSVGRR62 pKa = 11.84AVASASGAALGFIHH76 pKa = 6.57MNVPGAVASYY86 pKa = 10.25KK87 pKa = 10.42KK88 pKa = 10.49AGEE91 pKa = 4.24FYY93 pKa = 10.73DD94 pKa = 3.75YY95 pKa = 10.4MNSKK99 pKa = 8.38TVSQTKK105 pKa = 10.03SSSNNMYY112 pKa = 9.93SGKK115 pKa = 9.59FRR117 pKa = 11.84KK118 pKa = 9.27PKK120 pKa = 9.85PFKK123 pKa = 10.15QSAMMKK129 pKa = 9.69AQTDD133 pKa = 4.83GYY135 pKa = 10.96LSTQEE140 pKa = 3.92QYY142 pKa = 10.32GTVTDD147 pKa = 4.23PNGVYY152 pKa = 10.32IFQSTFNSAQMALAITTALVRR173 pKa = 11.84KK174 pKa = 9.16LFKK177 pKa = 10.56KK178 pKa = 10.65AGITVSDD185 pKa = 3.95RR186 pKa = 11.84DD187 pKa = 3.33GSLAFFGVYY196 pKa = 9.96NADD199 pKa = 3.57GFKK202 pKa = 11.12LEE204 pKa = 4.13MVEE207 pKa = 4.12YY208 pKa = 11.02NPITGANSPAITYY221 pKa = 6.98TTVAADD227 pKa = 4.3TITTVVANSTFVTTFTNYY245 pKa = 9.92LNKK248 pKa = 9.58ATEE251 pKa = 4.26HH252 pKa = 6.87PPVSMTLYY260 pKa = 10.5SSDD263 pKa = 4.42RR264 pKa = 11.84NGLDD268 pKa = 3.59SNWRR272 pKa = 11.84LCSNLDD278 pKa = 3.58LNAEE282 pKa = 4.21YY283 pKa = 9.15LTVVANSTMVLQNQTKK299 pKa = 10.28AVISASNDD307 pKa = 2.37IDD309 pKa = 4.7RR310 pKa = 11.84NDD312 pKa = 3.57VQPLNVTMYY321 pKa = 10.71KK322 pKa = 10.51FKK324 pKa = 10.99NDD326 pKa = 3.79PKK328 pKa = 10.86LKK330 pKa = 10.61AIGNRR335 pKa = 11.84PSGSSKK341 pKa = 9.87TEE343 pKa = 4.51LIQWQGVGYY352 pKa = 10.29SGTRR356 pKa = 11.84LLRR359 pKa = 11.84AQDD362 pKa = 3.68FVNTSFQNPPPPGIFSNLTGKK383 pKa = 9.31SHH385 pKa = 6.49STLQPGQMKK394 pKa = 10.67KK395 pKa = 10.47NFLTHH400 pKa = 5.34TFKK403 pKa = 11.33GKK405 pKa = 10.35LSNILPKK412 pKa = 9.9MRR414 pKa = 11.84MEE416 pKa = 4.18QFGLSPIKK424 pKa = 10.53IHH426 pKa = 6.86GVYY429 pKa = 10.69CNTNLFCFQEE439 pKa = 4.39RR440 pKa = 11.84MRR442 pKa = 11.84TSTTNPIIVAYY453 pKa = 8.52EE454 pKa = 3.89HH455 pKa = 6.75EE456 pKa = 4.35LDD458 pKa = 3.95IGVFCATYY466 pKa = 9.53HH467 pKa = 6.42PKK469 pKa = 10.07IAMQASLDD477 pKa = 3.97SMDD480 pKa = 3.2VSLL483 pKa = 5.7

Molecular weight: 53.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

767

284

483

383.5

43.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.476 ± 1.4

1.434 ± 0.625

5.215 ± 1.109

3.781 ± 1.552

4.302 ± 0.26

6.519 ± 0.104

1.304 ± 0.15

4.824 ± 0.705

6.649 ± 0.025

6.91 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.868 ± 0.672

5.997 ± 0.862

4.433 ± 0.126

4.302 ± 0.381

5.346 ± 0.602

9.387 ± 2.064

8.605 ± 0.948

6.389 ± 0.183

1.173 ± 0.784

5.085 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski