Amino acid dipepetide frequency for Pusillimonas sp. EA3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.6AlaAla: 11.6 ± 0.134
1.108AlaCys: 1.108 ± 0.04
5.389AlaAsp: 5.389 ± 0.067
5.516AlaGlu: 5.516 ± 0.08
3.916AlaPhe: 3.916 ± 0.075
8.775AlaGly: 8.775 ± 0.122
2.381AlaHis: 2.381 ± 0.058
5.469AlaIle: 5.469 ± 0.08
3.598AlaLys: 3.598 ± 0.081
12.529AlaLeu: 12.529 ± 0.139
3.149AlaMet: 3.149 ± 0.068
3.288AlaAsn: 3.288 ± 0.06
4.344AlaPro: 4.344 ± 0.076
5.401AlaGln: 5.401 ± 0.082
6.51AlaArg: 6.51 ± 0.093
5.665AlaSer: 5.665 ± 0.078
5.092AlaThr: 5.092 ± 0.085
7.652AlaVal: 7.652 ± 0.109
1.495AlaTrp: 1.495 ± 0.04
2.641AlaTyr: 2.641 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
0.959CysAla: 0.959 ± 0.032
0.112CysCys: 0.112 ± 0.013
0.506CysAsp: 0.506 ± 0.023
0.522CysGlu: 0.522 ± 0.022
0.355CysPhe: 0.355 ± 0.019
0.878CysGly: 0.878 ± 0.03
0.264CysHis: 0.264 ± 0.017
0.459CysIle: 0.459 ± 0.021
0.245CysLys: 0.245 ± 0.016
0.884CysLeu: 0.884 ± 0.028
0.202CysMet: 0.202 ± 0.014
0.272CysAsn: 0.272 ± 0.018
0.523CysPro: 0.523 ± 0.025
0.28CysGln: 0.28 ± 0.017
0.574CysArg: 0.574 ± 0.027
0.497CysSer: 0.497 ± 0.024
0.46CysThr: 0.46 ± 0.022
0.756CysVal: 0.756 ± 0.031
0.143CysTrp: 0.143 ± 0.013
0.23CysTyr: 0.23 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
5.583AspAla: 5.583 ± 0.089
0.427AspCys: 0.427 ± 0.022
2.852AspAsp: 2.852 ± 0.075
3.264AspGlu: 3.264 ± 0.058
2.007AspPhe: 2.007 ± 0.044
3.772AspGly: 3.772 ± 0.069
1.145AspHis: 1.145 ± 0.039
3.246AspIle: 3.246 ± 0.065
1.864AspLys: 1.864 ± 0.054
5.219AspLeu: 5.219 ± 0.084
1.37AspMet: 1.37 ± 0.037
1.703AspAsn: 1.703 ± 0.043
2.958AspPro: 2.958 ± 0.055
2.055AspGln: 2.055 ± 0.049
2.934AspArg: 2.934 ± 0.056
2.433AspSer: 2.433 ± 0.049
2.987AspThr: 2.987 ± 0.067
3.865AspVal: 3.865 ± 0.067
0.894AspTrp: 0.894 ± 0.032
1.557AspTyr: 1.557 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
5.968GluAla: 5.968 ± 0.093
0.417GluCys: 0.417 ± 0.022
2.398GluAsp: 2.398 ± 0.051
3.071GluGlu: 3.071 ± 0.077
1.853GluPhe: 1.853 ± 0.045
3.932GluGly: 3.932 ± 0.066
1.48GluHis: 1.48 ± 0.044
3.257GluIle: 3.257 ± 0.07
2.616GluLys: 2.616 ± 0.055
5.607GluLeu: 5.607 ± 0.094
1.534GluMet: 1.534 ± 0.042
2.111GluAsn: 2.111 ± 0.043
2.653GluPro: 2.653 ± 0.065
3.194GluGln: 3.194 ± 0.054
4.128GluArg: 4.128 ± 0.072
3.266GluSer: 3.266 ± 0.058
3.386GluThr: 3.386 ± 0.06
4.114GluVal: 4.114 ± 0.077
0.815GluTrp: 0.815 ± 0.034
1.422GluTyr: 1.422 ± 0.039
0.0GluXaa: 0.0 ± 0.0
Phe
3.672PheAla: 3.672 ± 0.066
0.409PheCys: 0.409 ± 0.022
2.381PheAsp: 2.381 ± 0.054
2.28PheGlu: 2.28 ± 0.048
1.453PhePhe: 1.453 ± 0.05
3.431PheGly: 3.431 ± 0.071
0.749PheHis: 0.749 ± 0.027
2.085PheIle: 2.085 ± 0.053
1.302PheLys: 1.302 ± 0.038
3.402PheLeu: 3.402 ± 0.068
0.98PheMet: 0.98 ± 0.037
1.462PheAsn: 1.462 ± 0.036
1.658PhePro: 1.658 ± 0.039
1.214PheGln: 1.214 ± 0.037
1.897PheArg: 1.897 ± 0.04
2.646PheSer: 2.646 ± 0.059
2.009PheThr: 2.009 ± 0.054
2.816PheVal: 2.816 ± 0.053
0.587PheTrp: 0.587 ± 0.029
1.113PheTyr: 1.113 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
7.583GlyAla: 7.583 ± 0.111
0.836GlyCys: 0.836 ± 0.029
3.66GlyAsp: 3.66 ± 0.066
4.03GlyGlu: 4.03 ± 0.071
3.317GlyPhe: 3.317 ± 0.062
6.059GlyGly: 6.059 ± 0.166
1.94GlyHis: 1.94 ± 0.048
4.413GlyIle: 4.413 ± 0.078
3.596GlyLys: 3.596 ± 0.063
8.709GlyLeu: 8.709 ± 0.117
2.371GlyMet: 2.371 ± 0.046
2.444GlyAsn: 2.444 ± 0.053
2.914GlyPro: 2.914 ± 0.056
3.297GlyGln: 3.297 ± 0.062
4.926GlyArg: 4.926 ± 0.077
4.437GlySer: 4.437 ± 0.07
4.053GlyThr: 4.053 ± 0.076
6.549GlyVal: 6.549 ± 0.091
1.247GlyTrp: 1.247 ± 0.04
2.433GlyTyr: 2.433 ± 0.043
0.0GlyXaa: 0.0 ± 0.0
His
2.429HisAla: 2.429 ± 0.061
0.264HisCys: 0.264 ± 0.017
1.351HisAsp: 1.351 ± 0.039
1.306HisGlu: 1.306 ± 0.042
1.022HisPhe: 1.022 ± 0.035
1.858HisGly: 1.858 ± 0.046
0.728HisHis: 0.728 ± 0.035
1.276HisIle: 1.276 ± 0.035
0.653HisLys: 0.653 ± 0.025
2.086HisLeu: 2.086 ± 0.055
0.532HisMet: 0.532 ± 0.024
0.76HisAsn: 0.76 ± 0.031
1.42HisPro: 1.42 ± 0.043
0.86HisGln: 0.86 ± 0.029
1.269HisArg: 1.269 ± 0.044
1.215HisSer: 1.215 ± 0.034
1.22HisThr: 1.22 ± 0.033
1.594HisVal: 1.594 ± 0.042
0.373HisTrp: 0.373 ± 0.015
0.802HisTyr: 0.802 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
5.867IleAla: 5.867 ± 0.079
0.488IleCys: 0.488 ± 0.023
3.482IleAsp: 3.482 ± 0.062
3.689IleGlu: 3.689 ± 0.067
1.687IlePhe: 1.687 ± 0.042
4.461IleGly: 4.461 ± 0.085
1.117IleHis: 1.117 ± 0.036
2.524IleIle: 2.524 ± 0.059
1.956IleLys: 1.956 ± 0.053
4.598IleLeu: 4.598 ± 0.081
1.224IleMet: 1.224 ± 0.041
1.93IleAsn: 1.93 ± 0.048
2.497IlePro: 2.497 ± 0.053
2.004IleGln: 2.004 ± 0.048
3.002IleArg: 3.002 ± 0.058
3.248IleSer: 3.248 ± 0.061
3.154IleThr: 3.154 ± 0.055
4.027IleVal: 4.027 ± 0.069
0.654IleTrp: 0.654 ± 0.029
1.221IleTyr: 1.221 ± 0.04
0.0IleXaa: 0.0 ± 0.0
Lys
4.086LysAla: 4.086 ± 0.083
0.198LysCys: 0.198 ± 0.014
1.754LysAsp: 1.754 ± 0.042
2.19LysGlu: 2.19 ± 0.047
0.967LysPhe: 0.967 ± 0.031
2.844LysGly: 2.844 ± 0.053
0.79LysHis: 0.79 ± 0.029
1.82LysIle: 1.82 ± 0.047
1.693LysLys: 1.693 ± 0.063
3.743LysLeu: 3.743 ± 0.059
0.958LysMet: 0.958 ± 0.033
1.259LysAsn: 1.259 ± 0.04
2.171LysPro: 2.171 ± 0.054
1.625LysGln: 1.625 ± 0.043
2.446LysArg: 2.446 ± 0.056
2.015LysSer: 2.015 ± 0.049
2.248LysThr: 2.248 ± 0.047
2.905LysVal: 2.905 ± 0.068
0.496LysTrp: 0.496 ± 0.021
0.813LysTyr: 0.813 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
12.073LeuAla: 12.073 ± 0.142
1.018LeuCys: 1.018 ± 0.029
5.385LeuAsp: 5.385 ± 0.072
5.893LeuGlu: 5.893 ± 0.092
4.016LeuPhe: 4.016 ± 0.088
8.293LeuGly: 8.293 ± 0.129
2.232LeuHis: 2.232 ± 0.058
5.402LeuIle: 5.402 ± 0.081
4.24LeuLys: 4.24 ± 0.064
10.631LeuLeu: 10.631 ± 0.153
2.671LeuMet: 2.671 ± 0.055
3.831LeuAsn: 3.831 ± 0.071
5.337LeuPro: 5.337 ± 0.072
4.002LeuGln: 4.002 ± 0.062
6.446LeuArg: 6.446 ± 0.089
6.96LeuSer: 6.96 ± 0.092
5.841LeuThr: 5.841 ± 0.076
7.51LeuVal: 7.51 ± 0.095
1.214LeuTrp: 1.214 ± 0.039
2.321LeuTyr: 2.321 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
2.921MetAla: 2.921 ± 0.06
0.192MetCys: 0.192 ± 0.014
1.042MetAsp: 1.042 ± 0.035
1.154MetGlu: 1.154 ± 0.035
0.773MetPhe: 0.773 ± 0.03
2.15MetGly: 2.15 ± 0.051
0.571MetHis: 0.571 ± 0.026
1.205MetIle: 1.205 ± 0.036
1.178MetLys: 1.178 ± 0.037
2.853MetLeu: 2.853 ± 0.06
0.71MetMet: 0.71 ± 0.031
0.993MetAsn: 0.993 ± 0.034
1.536MetPro: 1.536 ± 0.043
1.214MetGln: 1.214 ± 0.034
1.665MetArg: 1.665 ± 0.044
1.862MetSer: 1.862 ± 0.042
1.665MetThr: 1.665 ± 0.048
1.931MetVal: 1.931 ± 0.054
0.221MetTrp: 0.221 ± 0.016
0.426MetTyr: 0.426 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
3.711AsnAla: 3.711 ± 0.068
0.29AsnCys: 0.29 ± 0.017
1.914AsnAsp: 1.914 ± 0.04
2.064AsnGlu: 2.064 ± 0.051
1.11AsnPhe: 1.11 ± 0.036
2.853AsnGly: 2.853 ± 0.051
0.727AsnHis: 0.727 ± 0.029
1.841AsnIle: 1.841 ± 0.047
1.145AsnLys: 1.145 ± 0.033
3.365AsnLeu: 3.365 ± 0.067
0.86AsnMet: 0.86 ± 0.028
1.256AsnAsn: 1.256 ± 0.036
2.186AsnPro: 2.186 ± 0.051
1.379AsnGln: 1.379 ± 0.042
2.048AsnArg: 2.048 ± 0.045
1.583AsnSer: 1.583 ± 0.046
1.962AsnThr: 1.962 ± 0.043
2.447AsnVal: 2.447 ± 0.058
0.556AsnTrp: 0.556 ± 0.024
0.864AsnTyr: 0.864 ± 0.03
0.0AsnXaa: 0.0 ± 0.0
Pro
4.805ProAla: 4.805 ± 0.078
0.362ProCys: 0.362 ± 0.021
3.39ProAsp: 3.39 ± 0.064
3.849ProGlu: 3.849 ± 0.072
1.934ProPhe: 1.934 ± 0.047
3.95ProGly: 3.95 ± 0.067
1.087ProHis: 1.087 ± 0.03
2.315ProIle: 2.315 ± 0.051
1.627ProLys: 1.627 ± 0.049
4.671ProLeu: 4.671 ± 0.061
1.22ProMet: 1.22 ± 0.035
1.851ProAsn: 1.851 ± 0.045
2.098ProPro: 2.098 ± 0.049
1.882ProGln: 1.882 ± 0.043
2.322ProArg: 2.322 ± 0.06
2.7ProSer: 2.7 ± 0.052
2.417ProThr: 2.417 ± 0.045
4.126ProVal: 4.126 ± 0.071
0.721ProTrp: 0.721 ± 0.029
1.303ProTyr: 1.303 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
5.575GlnAla: 5.575 ± 0.089
0.357GlnCys: 0.357 ± 0.018
1.765GlnAsp: 1.765 ± 0.045
2.175GlnGlu: 2.175 ± 0.05
1.511GlnPhe: 1.511 ± 0.036
3.161GlnGly: 3.161 ± 0.061
0.968GlnHis: 0.968 ± 0.033
2.118GlnIle: 2.118 ± 0.051
1.51GlnLys: 1.51 ± 0.041
4.269GlnLeu: 4.269 ± 0.07
1.097GlnMet: 1.097 ± 0.036
1.39GlnAsn: 1.39 ± 0.043
2.093GlnPro: 2.093 ± 0.05
2.043GlnGln: 2.043 ± 0.047
2.909GlnArg: 2.909 ± 0.055
2.383GlnSer: 2.383 ± 0.054
2.463GlnThr: 2.463 ± 0.057
3.109GlnVal: 3.109 ± 0.051
0.729GlnTrp: 0.729 ± 0.031
1.018GlnTyr: 1.018 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
5.782ArgAla: 5.782 ± 0.079
0.5ArgCys: 0.5 ± 0.02
3.068ArgAsp: 3.068 ± 0.059
3.771ArgGlu: 3.771 ± 0.072
2.559ArgPhe: 2.559 ± 0.054
3.655ArgGly: 3.655 ± 0.057
1.67ArgHis: 1.67 ± 0.048
3.616ArgIle: 3.616 ± 0.072
2.423ArgLys: 2.423 ± 0.06
6.965ArgLeu: 6.965 ± 0.103
1.71ArgMet: 1.71 ± 0.043
2.225ArgAsn: 2.225 ± 0.05
2.65ArgPro: 2.65 ± 0.056
2.755ArgGln: 2.755 ± 0.058
4.247ArgArg: 4.247 ± 0.076
3.272ArgSer: 3.272 ± 0.054
2.985ArgThr: 2.985 ± 0.062
4.601ArgVal: 4.601 ± 0.07
0.916ArgTrp: 0.916 ± 0.032
1.967ArgTyr: 1.967 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
5.846SerAla: 5.846 ± 0.07
0.473SerCys: 0.473 ± 0.024
2.944SerAsp: 2.944 ± 0.057
2.964SerGlu: 2.964 ± 0.056
2.344SerPhe: 2.344 ± 0.052
5.398SerGly: 5.398 ± 0.076
1.247SerHis: 1.247 ± 0.037
3.054SerIle: 3.054 ± 0.058
1.803SerLys: 1.803 ± 0.038
6.231SerLeu: 6.231 ± 0.1
1.523SerMet: 1.523 ± 0.044
1.927SerAsn: 1.927 ± 0.045
2.763SerPro: 2.763 ± 0.055
2.271SerGln: 2.271 ± 0.047
3.602SerArg: 3.602 ± 0.059
3.526SerSer: 3.526 ± 0.072
3.057SerThr: 3.057 ± 0.056
4.526SerVal: 4.526 ± 0.079
0.84SerTrp: 0.84 ± 0.028
1.45SerTyr: 1.45 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
5.066ThrAla: 5.066 ± 0.084
0.404ThrCys: 0.404 ± 0.024
2.793ThrAsp: 2.793 ± 0.058
2.897ThrGlu: 2.897 ± 0.049
1.954ThrPhe: 1.954 ± 0.046
4.63ThrGly: 4.63 ± 0.078
1.377ThrHis: 1.377 ± 0.043
2.764ThrIle: 2.764 ± 0.057
1.415ThrLys: 1.415 ± 0.038
6.587ThrLeu: 6.587 ± 0.107
1.188ThrMet: 1.188 ± 0.039
1.499ThrAsn: 1.499 ± 0.039
3.344ThrPro: 3.344 ± 0.056
2.509ThrGln: 2.509 ± 0.054
3.151ThrArg: 3.151 ± 0.057
2.911ThrSer: 2.911 ± 0.055
2.911ThrThr: 2.911 ± 0.074
4.488ThrVal: 4.488 ± 0.076
0.7ThrTrp: 0.7 ± 0.024
1.308ThrTyr: 1.308 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
8.154ValAla: 8.154 ± 0.103
0.824ValCys: 0.824 ± 0.03
4.04ValAsp: 4.04 ± 0.077
4.401ValGlu: 4.401 ± 0.073
2.975ValPhe: 2.975 ± 0.056
5.602ValGly: 5.602 ± 0.077
1.545ValHis: 1.545 ± 0.042
4.116ValIle: 4.116 ± 0.073
2.836ValLys: 2.836 ± 0.056
8.223ValLeu: 8.223 ± 0.104
2.031ValMet: 2.031 ± 0.049
2.727ValAsn: 2.727 ± 0.051
3.518ValPro: 3.518 ± 0.058
2.852ValGln: 2.852 ± 0.058
4.401ValArg: 4.401 ± 0.076
4.915ValSer: 4.915 ± 0.067
4.009ValThr: 4.009 ± 0.064
6.535ValVal: 6.535 ± 0.099
1.025ValTrp: 1.025 ± 0.032
1.853ValTyr: 1.853 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
1.181TrpAla: 1.181 ± 0.041
0.167TrpCys: 0.167 ± 0.013
0.535TrpAsp: 0.535 ± 0.024
0.642TrpGlu: 0.642 ± 0.026
0.632TrpPhe: 0.632 ± 0.025
0.905TrpGly: 0.905 ± 0.029
0.409TrpHis: 0.409 ± 0.019
0.714TrpIle: 0.714 ± 0.028
0.493TrpLys: 0.493 ± 0.022
2.08TrpLeu: 2.08 ± 0.06
0.403TrpMet: 0.403 ± 0.021
0.458TrpAsn: 0.458 ± 0.02
0.658TrpPro: 0.658 ± 0.025
0.744TrpGln: 0.744 ± 0.027
1.091TrpArg: 1.091 ± 0.039
0.833TrpSer: 0.833 ± 0.033
0.588TrpThr: 0.588 ± 0.023
1.149TrpVal: 1.149 ± 0.032
0.252TrpTrp: 0.252 ± 0.019
0.367TrpTyr: 0.367 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.662TyrAla: 2.662 ± 0.059
0.273TyrCys: 0.273 ± 0.017
1.485TyrAsp: 1.485 ± 0.037
1.542TyrGlu: 1.542 ± 0.043
1.16TyrPhe: 1.16 ± 0.037
2.206TyrGly: 2.206 ± 0.045
0.594TyrHis: 0.594 ± 0.027
1.137TyrIle: 1.137 ± 0.038
0.825TyrLys: 0.825 ± 0.03
2.665TyrLeu: 2.665 ± 0.058
0.526TyrMet: 0.526 ± 0.022
0.774TyrAsn: 0.774 ± 0.026
1.308TyrPro: 1.308 ± 0.034
1.063TyrGln: 1.063 ± 0.039
1.77TyrArg: 1.77 ± 0.044
1.419TyrSer: 1.419 ± 0.04
1.392TyrThr: 1.392 ± 0.037
1.884TyrVal: 1.884 ± 0.045
0.425TyrTrp: 0.425 ± 0.022
0.75TyrTyr: 0.75 ± 0.031
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2949 proteins (975259 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski