Fusarium longipes
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11420 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A395SG12|A0A395SG12_9HYPO Amine oxidase (Fragment) OS=Fusarium longipes OX=694270 GN=FLONG3_7157 PE=3 SV=1
MM1 pKa = 6.98 PTATEE6 pKa = 4.01 FFGYY10 pKa = 9.49 SAHH13 pKa = 6.62 NLGPLTTTYY22 pKa = 8.12 TAPSSCATGTEE33 pKa = 3.62 HH34 pKa = 7.55 RR35 pKa = 11.84 VFVNASDD42 pKa = 3.75 PVRR45 pKa = 11.84 FFAAPTCGRR54 pKa = 11.84 QTFGDD59 pKa = 4.33 CLPSGSSWDD68 pKa = 3.85 SIKK71 pKa = 10.69 QQTTEE76 pKa = 4.02 FVQGVNFYY84 pKa = 9.44 FSPGIACPSGWRR96 pKa = 11.84 TVGTLAHH103 pKa = 7.4 DD104 pKa = 5.03 DD105 pKa = 3.94 DD106 pKa = 5.82 DD107 pKa = 4.3 KK108 pKa = 12.01 VSAFGALATPKK119 pKa = 9.87 WDD121 pKa = 4.02 DD122 pKa = 3.81 LEE124 pKa = 4.66 GAVNLLHH131 pKa = 6.47 PTEE134 pKa = 4.27 FWYY137 pKa = 10.81 GLLEE141 pKa = 4.29 PSEE144 pKa = 4.39 TLALCCPSNYY154 pKa = 9.91 KK155 pKa = 10.34 ADD157 pKa = 3.87 AYY159 pKa = 10.35 GDD161 pKa = 4.5 CISTIGPTEE170 pKa = 4.11 SFNYY174 pKa = 9.4 TAMCMTYY181 pKa = 10.21 RR182 pKa = 11.84 EE183 pKa = 4.41 LNGVPISTWDD193 pKa = 3.24 GTTLDD198 pKa = 4.03 RR199 pKa = 11.84 NQLISIASGTDD210 pKa = 3.28 EE211 pKa = 4.84 PRR213 pKa = 11.84 TTVDD217 pKa = 6.26 DD218 pKa = 3.11 IWLTNTQLDD227 pKa = 4.11 WAVATYY233 pKa = 10.59 VPAVPLIYY241 pKa = 10.32 KK242 pKa = 10.47 KK243 pKa = 10.35 SDD245 pKa = 3.05 KK246 pKa = 10.66 DD247 pKa = 3.84 GKK249 pKa = 10.69 DD250 pKa = 3.42 GDD252 pKa = 5.59 DD253 pKa = 5.98 DD254 pKa = 7.06 DD255 pKa = 7.77 DD256 pKa = 7.6 DD257 pKa = 7.6 DD258 pKa = 6.89 DD259 pKa = 7.14 DD260 pKa = 6.45 NDD262 pKa = 5.65 DD263 pKa = 4.73 GDD265 pKa = 5.05 DD266 pKa = 3.89 SAASTTTSRR275 pKa = 11.84 QEE277 pKa = 3.94 FVSVLGLTLGLLAGAGMLLL296 pKa = 3.73
Molecular weight: 31.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.884
IPC_protein 3.923
Toseland 3.681
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.872
Rodwell 3.732
Grimsley 3.592
Solomon 3.91
Lehninger 3.872
Nozaki 4.024
DTASelect 4.317
Thurlkill 3.745
EMBOSS 3.884
Sillero 4.037
Patrickios 1.303
IPC_peptide 3.91
IPC2_peptide 4.012
IPC2.peptide.svr19 3.908
Protein with the highest isoelectric point:
>tr|A0A395RZF7|A0A395RZF7_9HYPO Alpha beta fold family OS=Fusarium longipes OX=694270 GN=FLONG3_9225 PE=3 SV=1
MM1 pKa = 7.86 PLTRR5 pKa = 11.84 THH7 pKa = 6.29 RR8 pKa = 11.84 QSAPRR13 pKa = 11.84 RR14 pKa = 11.84 SIFSTRR20 pKa = 11.84 RR21 pKa = 11.84 HH22 pKa = 5.05 APARR26 pKa = 11.84 SSRR29 pKa = 11.84 HH30 pKa = 3.79 TTTTTTTTTTKK41 pKa = 9.91 PRR43 pKa = 11.84 RR44 pKa = 11.84 GMFGGGSTTRR54 pKa = 11.84 RR55 pKa = 11.84 THH57 pKa = 5.71 GSAPVHH63 pKa = 5.02 HH64 pKa = 6.65 HH65 pKa = 4.99 QRR67 pKa = 11.84 RR68 pKa = 11.84 PSMKK72 pKa = 10.02 DD73 pKa = 2.95 KK74 pKa = 11.41 VSGALLKK81 pKa = 11.04 LKK83 pKa = 10.68 GSLTRR88 pKa = 11.84 RR89 pKa = 11.84 PGVKK93 pKa = 9.89 AAGTRR98 pKa = 11.84 RR99 pKa = 11.84 MHH101 pKa = 5.65 GTDD104 pKa = 2.95 GRR106 pKa = 11.84 GARR109 pKa = 11.84 HH110 pKa = 5.73 RR111 pKa = 11.84 RR112 pKa = 11.84 YY113 pKa = 10.37
Molecular weight: 12.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.424
IPC2_protein 11.008
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.281
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.003
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11420
0
11420
5842010
38
13304
511.6
56.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.289 ± 0.021
1.24 ± 0.008
5.964 ± 0.02
6.301 ± 0.025
3.75 ± 0.015
6.732 ± 0.023
2.345 ± 0.01
5.101 ± 0.017
5.118 ± 0.023
8.656 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.225 ± 0.009
3.863 ± 0.011
5.897 ± 0.025
4.035 ± 0.017
5.807 ± 0.018
8.138 ± 0.024
6.081 ± 0.022
6.144 ± 0.015
1.514 ± 0.009
2.799 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here