Edwardsiella phage Edno5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Gofduovirus; unclassified Gofduovirus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3BYD3|A0A3G3BYD3_9CAUD Uncharacterized protein OS=Edwardsiella phage Edno5 OX=2419942 GN=Edno5_0059 PE=4 SV=1
MM1 pKa = 6.41MTVSIQPLKK10 pKa = 10.11SQSFSVQLGGQQCEE24 pKa = 3.49IRR26 pKa = 11.84LIQRR30 pKa = 11.84VSALYY35 pKa = 9.69MDD37 pKa = 4.65LTVDD41 pKa = 4.76GNPIMQGVPCYY52 pKa = 10.05YY53 pKa = 8.16GNRR56 pKa = 11.84MVRR59 pKa = 11.84YY60 pKa = 9.3SYY62 pKa = 11.26LGFKK66 pKa = 10.4GDD68 pKa = 5.16LVFLDD73 pKa = 4.26TDD75 pKa = 4.04GQSDD79 pKa = 4.23PTWDD83 pKa = 3.69GLGDD87 pKa = 4.16RR88 pKa = 11.84YY89 pKa = 10.82QLFYY93 pKa = 11.35LEE95 pKa = 4.97EE96 pKa = 5.43SDD98 pKa = 5.34LVV100 pKa = 3.67

Molecular weight: 11.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3BYD7|A0A3G3BYD7_9CAUD Uncharacterized protein OS=Edwardsiella phage Edno5 OX=2419942 GN=Edno5_0040 PE=4 SV=1
MM1 pKa = 7.43RR2 pKa = 11.84HH3 pKa = 6.9IIRR6 pKa = 11.84NAKK9 pKa = 9.22SDD11 pKa = 3.68KK12 pKa = 10.3EE13 pKa = 3.94RR14 pKa = 11.84AAAQAALSRR23 pKa = 11.84HH24 pKa = 4.36QQTFGDD30 pKa = 3.72YY31 pKa = 10.18GAQKK35 pKa = 8.98KK36 pKa = 6.62TFIYY40 pKa = 10.01RR41 pKa = 11.84VKK43 pKa = 10.81LGDD46 pKa = 3.34TVIPVDD52 pKa = 3.41IVNRR56 pKa = 11.84RR57 pKa = 11.84CSYY60 pKa = 10.24VATVRR65 pKa = 11.84NGHH68 pKa = 6.46IGLGRR73 pKa = 11.84VGG75 pKa = 3.7

Molecular weight: 8.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

12744

38

585

167.7

18.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.055 ± 0.325

1.405 ± 0.139

5.705 ± 0.232

6.105 ± 0.357

3.688 ± 0.174

7.258 ± 0.349

1.365 ± 0.149

6.332 ± 0.231

5.807 ± 0.281

7.706 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.021 ± 0.207

4.716 ± 0.218

4.229 ± 0.181

3.994 ± 0.208

5.438 ± 0.357

7.313 ± 0.282

5.54 ± 0.292

6.513 ± 0.273

1.554 ± 0.136

3.256 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski