Quadrisphaera sp. DD2A

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Kineosporiales; Kineosporiaceae; Quadrisphaera; unclassified Quadrisphaera

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4416 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C8ZKH8|A0A5C8ZKH8_9ACTN PH domain-containing protein OS=Quadrisphaera sp. DD2A OX=2593304 GN=FMM08_04280 PE=4 SV=1
MM1 pKa = 7.71AATSLLVLTLGGCSSDD17 pKa = 3.59SDD19 pKa = 3.97LASRR23 pKa = 11.84NLSTEE28 pKa = 3.77AEE30 pKa = 4.11RR31 pKa = 11.84FEE33 pKa = 4.34VDD35 pKa = 3.15RR36 pKa = 11.84QIVFSNGVTDD46 pKa = 5.05AYY48 pKa = 11.1VLEE51 pKa = 4.25IQGRR55 pKa = 11.84CSVDD59 pKa = 3.09PAEE62 pKa = 4.9SLDD65 pKa = 3.7ALEE68 pKa = 4.68VTCEE72 pKa = 4.14VGDD75 pKa = 4.08GEE77 pKa = 4.75YY78 pKa = 10.34EE79 pKa = 3.92QHH81 pKa = 7.46LLGLSDD87 pKa = 3.3KK88 pKa = 7.39TTCFVEE94 pKa = 4.13QLEE97 pKa = 4.43SRR99 pKa = 11.84DD100 pKa = 3.11VSTYY104 pKa = 8.73HH105 pKa = 7.4DD106 pKa = 3.62EE107 pKa = 4.31VAFEE111 pKa = 4.54PEE113 pKa = 4.71SIVPDD118 pKa = 4.53DD119 pKa = 4.96DD120 pKa = 6.01LSTSSDD126 pKa = 3.22GGG128 pKa = 3.63

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C8ZFZ9|A0A5C8ZFZ9_9ACTN Aldo/keto reductase OS=Quadrisphaera sp. DD2A OX=2593304 GN=FMM08_11625 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.87GRR40 pKa = 11.84AQLSAA45 pKa = 3.87

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4416

0

4416

1464610

29

1733

331.7

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.554 ± 0.059

0.656 ± 0.01

5.944 ± 0.034

5.15 ± 0.034

2.286 ± 0.022

9.79 ± 0.035

2.125 ± 0.02

2.027 ± 0.025

1.278 ± 0.019

10.853 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.354 ± 0.012

1.195 ± 0.018

6.076 ± 0.031

2.866 ± 0.021

8.095 ± 0.044

5.559 ± 0.034

5.84 ± 0.03

10.265 ± 0.038

1.554 ± 0.013

1.534 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski