Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Salinispora; Salinispora tropica

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4522 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4X2H7|A4X2H7_SALTO Uncharacterized protein OS=Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) OX=369723 GN=Strop_0597 PE=4 SV=1
MM1 pKa = 7.4LNDD4 pKa = 3.22NATLVVGAGNFFRR17 pKa = 11.84APTGTIAPSDD27 pKa = 3.98LFSIDD32 pKa = 4.19SMWEE36 pKa = 4.2NVGHH40 pKa = 6.66TSLEE44 pKa = 4.49DD45 pKa = 3.06IFGITSEE52 pKa = 4.23GGEE55 pKa = 4.12ATTLGTLQAQTLRR68 pKa = 11.84TTYY71 pKa = 10.97APRR74 pKa = 11.84TEE76 pKa = 4.52SFAFTLQQFDD86 pKa = 3.55RR87 pKa = 11.84AGLRR91 pKa = 11.84LYY93 pKa = 10.53YY94 pKa = 10.59GSNAPLLADD103 pKa = 4.41GSLGVPMSPVPTEE116 pKa = 3.79CAFLVVFYY124 pKa = 10.95DD125 pKa = 3.58GTNAFAFYY133 pKa = 10.52APKK136 pKa = 10.85AEE138 pKa = 4.33IFRR141 pKa = 11.84ADD143 pKa = 3.94DD144 pKa = 3.91LSVSDD149 pKa = 4.6AEE151 pKa = 4.54SLAGLPLSVKK161 pKa = 10.13PLVFGSNAYY170 pKa = 10.14AYY172 pKa = 7.71TVTPIGGIEE181 pKa = 4.18AEE183 pKa = 4.51GASAGTPGAFSPDD196 pKa = 3.81GADD199 pKa = 2.96TPYY202 pKa = 11.5NLTALAGVTASPATAWTTGQYY223 pKa = 10.82VDD225 pKa = 5.58LEE227 pKa = 5.0DD228 pKa = 3.77GTTAYY233 pKa = 9.04WDD235 pKa = 3.5SSVWVAGVAAA245 pKa = 4.81

Molecular weight: 25.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4X0U3|A4X0U3_SALTO DNA gyrase subunit B OS=Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) OX=369723 GN=gyrB PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIISTRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.47GRR40 pKa = 11.84ARR42 pKa = 11.84LAAA45 pKa = 4.44

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4522

0

4522

1517537

33

7210

335.6

35.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.586 ± 0.056

0.79 ± 0.01

5.952 ± 0.032

5.232 ± 0.033

2.624 ± 0.019

9.328 ± 0.039

2.208 ± 0.019

3.291 ± 0.026

1.546 ± 0.025

10.527 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.629 ± 0.014

1.837 ± 0.023

6.227 ± 0.031

2.908 ± 0.018

8.431 ± 0.04

4.967 ± 0.033

6.302 ± 0.035

9.042 ± 0.039

1.558 ± 0.017

2.016 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski