African horse sickness virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D6R1D9|D6R1D9_9REOV Non-structural protein NS1 OS=African horse sickness virus OX=40050 PE=4 SV=1
MM1 pKa = 7.73DD2 pKa = 6.41AIAARR7 pKa = 11.84ALSVVRR13 pKa = 11.84ACVTVTDD20 pKa = 4.54ARR22 pKa = 11.84VSLDD26 pKa = 3.45PGVMEE31 pKa = 4.58TLGIAINRR39 pKa = 11.84YY40 pKa = 9.5NGLTNHH46 pKa = 5.97SVSMRR51 pKa = 11.84PQTQAEE57 pKa = 4.13RR58 pKa = 11.84NEE60 pKa = 4.13MFFMCTDD67 pKa = 3.34MVLAALNVQIGNISPDD83 pKa = 3.27YY84 pKa = 10.33DD85 pKa = 3.29QALATVGALATTEE98 pKa = 3.61IPYY101 pKa = 10.23NVQAMNDD108 pKa = 3.14IVRR111 pKa = 11.84ITGQMQTFGPSKK123 pKa = 10.5VQTGPYY129 pKa = 9.02AGAVEE134 pKa = 4.57VQQSGRR140 pKa = 11.84YY141 pKa = 7.76YY142 pKa = 10.0VPQGRR147 pKa = 11.84TRR149 pKa = 11.84GGYY152 pKa = 9.08INSNIAEE159 pKa = 4.2VCMDD163 pKa = 4.42AGAAGQVNALLAPRR177 pKa = 11.84RR178 pKa = 11.84GDD180 pKa = 2.91AVMIYY185 pKa = 8.43FVWRR189 pKa = 11.84PLRR192 pKa = 11.84IFCDD196 pKa = 3.62PQGASLEE203 pKa = 4.41SAPGTFVTVDD213 pKa = 3.69GVNVAAGDD221 pKa = 3.79VVAWNTIAPVNVGNPGARR239 pKa = 11.84RR240 pKa = 11.84SILQFEE246 pKa = 4.69VLWYY250 pKa = 9.47TSLDD254 pKa = 3.49RR255 pKa = 11.84SLDD258 pKa = 3.83TVPEE262 pKa = 4.24LAPTLTRR269 pKa = 11.84CYY271 pKa = 10.57AYY273 pKa = 10.75VSPTWHH279 pKa = 7.46ALRR282 pKa = 11.84AVIFQQMNMQPINPPIFPPTEE303 pKa = 3.78RR304 pKa = 11.84NEE306 pKa = 3.78IVAYY310 pKa = 10.61LLVASLADD318 pKa = 3.29VYY320 pKa = 11.39AALRR324 pKa = 11.84PDD326 pKa = 3.02FRR328 pKa = 11.84MNGVVAPVGQINRR341 pKa = 11.84ALVLAAYY348 pKa = 8.87HH349 pKa = 6.25

Molecular weight: 37.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4UUP0|B4UUP0_9REOV Outer capsid protein VP5 OS=African horse sickness virus OX=40050 GN=VP5 PE=3 SV=1
MM1 pKa = 7.32NLATIAKK8 pKa = 8.79NYY10 pKa = 10.04SMHH13 pKa = 6.55NGEE16 pKa = 4.51SGAIVPYY23 pKa = 10.42VPPPYY28 pKa = 10.35NFASAPTFSQRR39 pKa = 11.84TSQMEE44 pKa = 4.39SVSLGILNQAMSSTTGASGALKK66 pKa = 10.4DD67 pKa = 3.76EE68 pKa = 4.49KK69 pKa = 11.14AAFGAMAEE77 pKa = 4.23ALRR80 pKa = 11.84DD81 pKa = 3.88PEE83 pKa = 5.29PIRR86 pKa = 11.84QIKK89 pKa = 8.76KK90 pKa = 8.66QVGIRR95 pKa = 11.84TLKK98 pKa = 8.46NLKK101 pKa = 9.25MEE103 pKa = 5.0LATMRR108 pKa = 11.84RR109 pKa = 11.84KK110 pKa = 10.13KK111 pKa = 10.43SALKK115 pKa = 10.48IMIFISGCVTLATSMVGGLSIVDD138 pKa = 3.81DD139 pKa = 5.11EE140 pKa = 5.11ILRR143 pKa = 11.84DD144 pKa = 3.75YY145 pKa = 11.3KK146 pKa = 11.53NNDD149 pKa = 2.78WLMKK153 pKa = 9.64TIHH156 pKa = 6.52GLNLLCTTVLLAAGKK171 pKa = 10.19ISDD174 pKa = 4.01KK175 pKa = 9.65MQEE178 pKa = 4.12EE179 pKa = 4.24ISRR182 pKa = 11.84TKK184 pKa = 10.41RR185 pKa = 11.84DD186 pKa = 3.02IAKK189 pKa = 9.99RR190 pKa = 11.84EE191 pKa = 4.34SYY193 pKa = 11.01VSAASMSWSGDD204 pKa = 3.09TEE206 pKa = 4.02MLLQGIKK213 pKa = 10.53YY214 pKa = 10.44GEE216 pKa = 4.17SS217 pKa = 3.11

Molecular weight: 23.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6262

217

1305

626.2

71.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.547 ± 0.878

1.214 ± 0.183

6.196 ± 0.282

7.106 ± 0.676

3.881 ± 0.476

6.404 ± 0.812

1.996 ± 0.24

6.771 ± 0.414

5.957 ± 0.831

8.464 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.689 ± 0.206

4.104 ± 0.356

3.801 ± 0.458

3.338 ± 0.423

7.058 ± 0.291

6.052 ± 0.59

5.525 ± 0.313

6.707 ± 0.356

1.262 ± 0.192

3.928 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski