Perinet vesiculovirus
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I1SV89|I1SV89_9RHAB Matrix protein OS=Perinet vesiculovirus OX=1972569 PE=3 SV=1
MM1 pKa = 7.38 SRR3 pKa = 11.84 LLEE6 pKa = 4.18 TFKK9 pKa = 11.09 NYY11 pKa = 9.87 PNLEE15 pKa = 3.99 KK16 pKa = 10.38 TMEE19 pKa = 4.72 EE20 pKa = 3.92 INDD23 pKa = 3.84 IEE25 pKa = 5.14 DD26 pKa = 3.48 STSQEE31 pKa = 4.09 EE32 pKa = 4.48 KK33 pKa = 10.7 KK34 pKa = 9.33 VTLVLSEE41 pKa = 4.1 EE42 pKa = 3.97 PSYY45 pKa = 11.1 FLAHH49 pKa = 6.32 MLPSEE54 pKa = 4.04 EE55 pKa = 5.41 DD56 pKa = 3.33 EE57 pKa = 4.21 TDD59 pKa = 3.47 EE60 pKa = 4.75 EE61 pKa = 5.14 GGGQVDD67 pKa = 3.64 VNPYY71 pKa = 10.11 EE72 pKa = 4.43 DD73 pKa = 5.23 AEE75 pKa = 4.36 SSDD78 pKa = 3.67 LNEE81 pKa = 5.58 DD82 pKa = 3.49 EE83 pKa = 5.95 FEE85 pKa = 4.7 VNFDD89 pKa = 3.78 KK90 pKa = 11.28 KK91 pKa = 10.32 PWTALTEE98 pKa = 4.18 KK99 pKa = 10.22 VVKK102 pKa = 10.53 GNIRR106 pKa = 11.84 IDD108 pKa = 3.41 MMHH111 pKa = 7.33 PEE113 pKa = 4.69 GLTDD117 pKa = 3.67 AQFTQWVQGIQALLDD132 pKa = 3.6 ISHH135 pKa = 6.81 HH136 pKa = 6.19 VDD138 pKa = 3.74 LNRR141 pKa = 11.84 LKK143 pKa = 10.83 LGQTSDD149 pKa = 4.03 GIVLIEE155 pKa = 4.5 EE156 pKa = 4.28 KK157 pKa = 10.11 PKK159 pKa = 10.76 RR160 pKa = 11.84 PSSPSLKK167 pKa = 10.63 LEE169 pKa = 4.1 LKK171 pKa = 10.4 EE172 pKa = 4.24 FVSPDD177 pKa = 3.14 SRR179 pKa = 11.84 FGTPTDD185 pKa = 3.52 NSSEE189 pKa = 4.25 SSLGLPPTPEE199 pKa = 3.77 KK200 pKa = 11.27 VNWKK204 pKa = 7.61 QTVEE208 pKa = 4.14 IILEE212 pKa = 4.34 VKK214 pKa = 10.53 ALDD217 pKa = 4.36 PDD219 pKa = 3.83 LPSYY223 pKa = 9.29 KK224 pKa = 8.35 TTLSKK229 pKa = 11.12 LFGSQEE235 pKa = 3.86 AALQYY240 pKa = 10.49 CNSGSPMLKK249 pKa = 10.14 DD250 pKa = 3.67 AFIAGLKK257 pKa = 10.11 RR258 pKa = 11.84 KK259 pKa = 10.18 GIFNRR264 pKa = 11.84 MRR266 pKa = 11.84 TKK268 pKa = 10.89 YY269 pKa = 10.28 ILDD272 pKa = 3.59 PTLPMM277 pKa = 5.19
Molecular weight: 31.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.71
IPC2_protein 4.609
IPC_protein 4.546
Toseland 4.393
ProMoST 4.635
Dawson 4.482
Bjellqvist 4.635
Wikipedia 4.342
Rodwell 4.38
Grimsley 4.304
Solomon 4.482
Lehninger 4.431
Nozaki 4.584
DTASelect 4.724
Thurlkill 4.393
EMBOSS 4.355
Sillero 4.647
Patrickios 4.151
IPC_peptide 4.482
IPC2_peptide 4.647
IPC2.peptide.svr19 4.577
Protein with the highest isoelectric point:
>tr|I1SV90|I1SV90_9RHAB Glycoprotein OS=Perinet vesiculovirus OX=1972569 PE=3 SV=1
MM1 pKa = 7.32 NRR3 pKa = 11.84 IRR5 pKa = 11.84 KK6 pKa = 8.49 FWGSNKK12 pKa = 9.65 NSQSPEE18 pKa = 3.86 KK19 pKa = 10.28 KK20 pKa = 9.91 SKK22 pKa = 10.37 KK23 pKa = 9.51 KK24 pKa = 10.41 KK25 pKa = 9.93 KK26 pKa = 9.91 NNNNHH31 pKa = 5.6 GSQMEE36 pKa = 4.31 WDD38 pKa = 3.88 NPPTYY43 pKa = 10.73 SEE45 pKa = 4.13 AAKK48 pKa = 9.93 FDD50 pKa = 3.71 PSAPFFGLEE59 pKa = 4.03 PEE61 pKa = 4.44 DD62 pKa = 3.84 MEE64 pKa = 4.52 FNYY67 pKa = 10.64 YY68 pKa = 10.43 SGLASVKK75 pKa = 10.12 LQYY78 pKa = 10.89 KK79 pKa = 8.38 CTIQVRR85 pKa = 11.84 AKK87 pKa = 10.08 KK88 pKa = 10.03 PFVSFLDD95 pKa = 3.56 AAEE98 pKa = 4.67 NLSAWEE104 pKa = 3.92 NNYY107 pKa = 10.78 KK108 pKa = 10.7 GFLGKK113 pKa = 10.36 KK114 pKa = 7.74 PFYY117 pKa = 10.13 RR118 pKa = 11.84 AIILTTLKK126 pKa = 10.27 KK127 pKa = 10.73 LKK129 pKa = 10.28 VSPHH133 pKa = 6.3 SLMDD137 pKa = 4.07 GNAPEE142 pKa = 4.28 YY143 pKa = 10.58 NGEE146 pKa = 4.18 SEE148 pKa = 4.42 GRR150 pKa = 11.84 CLILHH155 pKa = 6.23 SLGIIPPMMYY165 pKa = 10.06 VPEE168 pKa = 4.07 QFTRR172 pKa = 11.84 EE173 pKa = 3.47 WSTRR177 pKa = 11.84 DD178 pKa = 3.21 NQGIISVRR186 pKa = 11.84 ISVGITDD193 pKa = 3.7 TVDD196 pKa = 3.1 NLEE199 pKa = 4.6 GLFSSPVFLDD209 pKa = 3.13 HH210 pKa = 7.58 KK211 pKa = 10.45 EE212 pKa = 4.2 LITAGRR218 pKa = 11.84 MFNLDD223 pKa = 3.58 LKK225 pKa = 11.0 VSPEE229 pKa = 4.0 NGWFISKK236 pKa = 10.23 SYY238 pKa = 10.99
Molecular weight: 27.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.576
IPC2_protein 8.463
IPC_protein 8.39
Toseland 9.136
ProMoST 8.975
Dawson 9.399
Bjellqvist 9.136
Wikipedia 9.545
Rodwell 9.677
Grimsley 9.458
Solomon 9.472
Lehninger 9.443
Nozaki 9.253
DTASelect 9.092
Thurlkill 9.253
EMBOSS 9.56
Sillero 9.37
Patrickios 4.838
IPC_peptide 9.472
IPC2_peptide 7.673
IPC2.peptide.svr19 7.692
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3458
238
2094
691.6
78.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.714 ± 0.765
1.764 ± 0.418
6.044 ± 0.525
5.726 ± 0.826
4.309 ± 0.255
5.87 ± 0.293
2.4 ± 0.247
6.449 ± 0.605
6.536 ± 0.565
9.919 ± 0.721
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.718 ± 0.407
4.685 ± 0.467
4.829 ± 0.852
3.21 ± 0.128
5.35 ± 0.742
8.618 ± 0.924
5.986 ± 0.588
5.552 ± 0.307
1.909 ± 0.189
3.412 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here