Emergomyces pasteurianus Ep9510

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Ajellomycetaceae; Emergomyces; Emergomyces pasteurianus

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8944 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J9PW76|A0A1J9PW76_9EURO Uncharacterized protein OS=Emergomyces pasteurianus Ep9510 OX=1447872 GN=AJ78_07210 PE=4 SV=1
MM1 pKa = 6.43TTPFAMEE8 pKa = 4.23RR9 pKa = 11.84LLLHH13 pKa = 6.61AGVPRR18 pKa = 11.84LHH20 pKa = 6.43TATRR24 pKa = 11.84PPQLNPTVTGPQSRR38 pKa = 11.84CDD40 pKa = 3.76PCNSTSDD47 pKa = 4.29DD48 pKa = 3.77EE49 pKa = 4.23QAEE52 pKa = 4.01ADD54 pKa = 3.84FEE56 pKa = 4.81VIEE59 pKa = 4.46VRR61 pKa = 11.84PSTDD65 pKa = 3.3DD66 pKa = 3.85GEE68 pKa = 4.35TDD70 pKa = 3.21EE71 pKa = 4.91MSDD74 pKa = 3.42VSSIFSDD81 pKa = 3.09IDD83 pKa = 4.52DD84 pKa = 4.41IEE86 pKa = 4.95DD87 pKa = 3.92PNPYY91 pKa = 9.98LFYY94 pKa = 10.73VIPLTEE100 pKa = 4.4EE101 pKa = 4.38PDD103 pKa = 4.04DD104 pKa = 4.6EE105 pKa = 6.53DD106 pKa = 5.87DD107 pKa = 4.23EE108 pKa = 5.47FILVIPYY115 pKa = 10.27QGDD118 pKa = 3.65NQPSLSTLLEE128 pKa = 4.29RR129 pKa = 11.84DD130 pKa = 3.58QIYY133 pKa = 9.39PWASPTNPDD142 pKa = 3.43PYY144 pKa = 10.77HH145 pKa = 6.46YY146 pKa = 10.77LNQDD150 pKa = 3.13DD151 pKa = 5.5VYY153 pKa = 9.88TDD155 pKa = 3.69HH156 pKa = 7.39SDD158 pKa = 3.55SVPSSMVNSAILGPYY173 pKa = 10.0DD174 pKa = 3.52FTANRR179 pKa = 11.84EE180 pKa = 4.08GSGEE184 pKa = 4.05DD185 pKa = 3.67MLWSMSMSSSSITSSRR201 pKa = 11.84MNCSGRR207 pKa = 11.84NGSASLSLKK216 pKa = 10.4YY217 pKa = 10.53AGTYY221 pKa = 10.48LDD223 pKa = 4.91DD224 pKa = 4.87LVDD227 pKa = 3.51GHH229 pKa = 6.32MEE231 pKa = 4.65GFPDD235 pKa = 3.58ILGMII240 pKa = 4.82

Molecular weight: 26.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J9PDG2|A0A1J9PDG2_9EURO BZIP domain-containing protein OS=Emergomyces pasteurianus Ep9510 OX=1447872 GN=AJ78_05029 PE=4 SV=1
MM1 pKa = 6.85GQAVQCHH8 pKa = 6.92LDD10 pKa = 3.19NFARR14 pKa = 11.84VITSNADD21 pKa = 2.92KK22 pKa = 11.07ARR24 pKa = 11.84AANRR28 pKa = 11.84AIILAMLHH36 pKa = 6.29TIRR39 pKa = 11.84SASRR43 pKa = 11.84TRR45 pKa = 11.84AGLRR49 pKa = 11.84TLGTFCIDD57 pKa = 3.76FPSPKK62 pKa = 10.13LILYY66 pKa = 7.7RR67 pKa = 11.84NPGSGAVGMTKK78 pKa = 10.24DD79 pKa = 3.36SCMLSGVIQGG89 pKa = 3.83

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8944

0

8944

4270530

31

5770

477.5

52.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.974 ± 0.024

1.198 ± 0.009

5.614 ± 0.018

6.209 ± 0.022

3.652 ± 0.017

6.734 ± 0.024

2.485 ± 0.012

5.098 ± 0.017

5.047 ± 0.02

8.834 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.085 ± 0.009

3.995 ± 0.015

6.286 ± 0.028

4.069 ± 0.02

6.298 ± 0.022

8.749 ± 0.03

5.934 ± 0.017

5.781 ± 0.02

1.293 ± 0.009

2.665 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski