Cyanophage P-RSM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4QGE7|M4QGE7_9CAUD DNA primase OS=Cyanophage P-RSM1 OX=536444 GN=CPPG_00119 PE=4 SV=1
MM1 pKa = 6.51TTAQRR6 pKa = 11.84FSSCLSILEE15 pKa = 4.45DD16 pKa = 3.54AVDD19 pKa = 3.87RR20 pKa = 11.84QIMLDD25 pKa = 3.1SDD27 pKa = 3.99YY28 pKa = 11.14PIIHH32 pKa = 6.05NQLVKK37 pKa = 10.32HH38 pKa = 5.49YY39 pKa = 9.32EE40 pKa = 3.81DD41 pKa = 4.38RR42 pKa = 11.84GVDD45 pKa = 3.84FYY47 pKa = 12.02GDD49 pKa = 3.16VDD51 pKa = 3.75EE52 pKa = 6.87DD53 pKa = 4.4YY54 pKa = 11.26DD55 pKa = 3.71ILLAKK60 pKa = 10.44LEE62 pKa = 4.28SDD64 pKa = 4.59LYY66 pKa = 11.53YY67 pKa = 10.66YY68 pKa = 10.86DD69 pKa = 3.6QDD71 pKa = 3.42

Molecular weight: 8.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4QQI5|M4QQI5_9CAUD Uncharacterized protein OS=Cyanophage P-RSM1 OX=536444 GN=CPPG_00043 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84VRR4 pKa = 11.84LEE6 pKa = 3.71PRR8 pKa = 11.84KK9 pKa = 10.13GNRR12 pKa = 11.84EE13 pKa = 3.88AEE15 pKa = 4.06EE16 pKa = 3.75MFRR19 pKa = 11.84YY20 pKa = 9.41HH21 pKa = 6.44LHH23 pKa = 6.34RR24 pKa = 11.84DD25 pKa = 3.77GYY27 pKa = 8.53VHH29 pKa = 5.03VTEE32 pKa = 5.74RR33 pKa = 11.84LDD35 pKa = 2.95RR36 pKa = 11.84WYY38 pKa = 11.11VFHH41 pKa = 7.61PNANTGFWVHH51 pKa = 5.82PTKK54 pKa = 10.88DD55 pKa = 4.05PNWIMHH61 pKa = 6.38KK62 pKa = 10.65

Molecular weight: 7.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

212

0

212

56307

51

5356

265.6

29.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.933 ± 0.237

0.902 ± 0.095

6.66 ± 0.125

5.974 ± 0.313

4.314 ± 0.108

7.972 ± 0.318

1.575 ± 0.111

5.98 ± 0.164

6.031 ± 0.405

7.024 ± 0.171

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.094 ± 0.208

5.886 ± 0.187

3.865 ± 0.147

3.765 ± 0.096

3.912 ± 0.153

7.139 ± 0.226

7.711 ± 0.478

6.777 ± 0.167

1.14 ± 0.086

4.344 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski