Acinetobacter phage AbKT21phiIII
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3T0IGI5|A0A3T0IGI5_9CAUD Capsid and scaffold protein OS=Acinetobacter phage AbKT21phiIII OX=2500826 PE=4 SV=1
MM1 pKa = 7.12 TNYY4 pKa = 9.44 VVKK7 pKa = 10.55 NAEE10 pKa = 4.23 LTTEE14 pKa = 3.85 QHH16 pKa = 7.2 DD17 pKa = 3.77 RR18 pKa = 11.84 LQGKK22 pKa = 8.83 GYY24 pKa = 10.71 KK25 pKa = 9.57 FDD27 pKa = 3.72 NTNGEE32 pKa = 4.39 DD33 pKa = 3.58 LTGGNHH39 pKa = 7.18 FIVSDD44 pKa = 4.69 RR45 pKa = 11.84 INEE48 pKa = 3.99 YY49 pKa = 9.23 STNANNLCLLTCGGFEE65 pKa = 4.63 LYY67 pKa = 10.62 EE68 pKa = 4.38 SVDD71 pKa = 3.38 QLIDD75 pKa = 3.4 VLPNVVGFVVGARR88 pKa = 11.84 VKK90 pKa = 10.92 LNNLQGNPKK99 pKa = 8.46 MFNYY103 pKa = 8.15 ATWSEE108 pKa = 4.0 AGFEE112 pKa = 4.14 LGAAGTIIAEE122 pKa = 3.77 VDD124 pKa = 3.58 YY125 pKa = 11.54 AGDD128 pKa = 3.48 VYY130 pKa = 11.58 VRR132 pKa = 11.84 FDD134 pKa = 3.61 TASHH138 pKa = 7.41 PDD140 pKa = 3.52 RR141 pKa = 11.84 DD142 pKa = 4.83 DD143 pKa = 3.83 KK144 pKa = 11.34 EE145 pKa = 4.33 LWVAVADD152 pKa = 5.56 LILEE156 pKa = 4.23
Molecular weight: 17.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.281
IPC2_protein 4.342
IPC_protein 4.266
Toseland 4.088
ProMoST 4.355
Dawson 4.24
Bjellqvist 4.431
Wikipedia 4.139
Rodwell 4.101
Grimsley 3.999
Solomon 4.228
Lehninger 4.19
Nozaki 4.355
DTASelect 4.546
Thurlkill 4.113
EMBOSS 4.151
Sillero 4.393
Patrickios 3.338
IPC_peptide 4.24
IPC2_peptide 4.38
IPC2.peptide.svr19 4.312
Protein with the highest isoelectric point:
>tr|A0A3T0IGH8|A0A3T0IGH8_9CAUD Uncharacterized protein OS=Acinetobacter phage AbKT21phiIII OX=2500826 PE=4 SV=1
MM1 pKa = 7.24 LTDD4 pKa = 5.66 CIDD7 pKa = 3.49 HH8 pKa = 6.82 GKK10 pKa = 7.95 TKK12 pKa = 10.55 SLSPEE17 pKa = 4.14 GYY19 pKa = 10.67 VMVLNPYY26 pKa = 9.32 KK27 pKa = 10.48 KK28 pKa = 9.45 PRR30 pKa = 11.84 ISRR33 pKa = 11.84 LHH35 pKa = 6.57 RR36 pKa = 11.84 IVYY39 pKa = 8.27 CQSNNIHH46 pKa = 5.74 MQDD49 pKa = 2.93 IEE51 pKa = 4.52 GLVVRR56 pKa = 11.84 HH57 pKa = 5.98 KK58 pKa = 10.65 CDD60 pKa = 3.02 NPRR63 pKa = 11.84 CVNPEE68 pKa = 3.68 HH69 pKa = 8.13 LEE71 pKa = 4.01 IGTLADD77 pKa = 3.68 NNKK80 pKa = 9.7 DD81 pKa = 3.07 RR82 pKa = 11.84 AKK84 pKa = 10.37 RR85 pKa = 11.84 GRR87 pKa = 11.84 STKK90 pKa = 10.08 VVPSKK95 pKa = 11.01 HH96 pKa = 6.41 KK97 pKa = 10.03 LTTEE101 pKa = 4.76 DD102 pKa = 2.87 ISTIRR107 pKa = 11.84 SRR109 pKa = 11.84 YY110 pKa = 8.98 IKK112 pKa = 10.66 GKK114 pKa = 9.46 AGKK117 pKa = 9.62 YY118 pKa = 9.36 NPNGYY123 pKa = 9.71 AALAKK128 pKa = 9.97 EE129 pKa = 4.26 YY130 pKa = 10.96 NVDD133 pKa = 3.19 EE134 pKa = 4.22 KK135 pKa = 11.47 VIYY138 pKa = 10.59 NVVKK142 pKa = 10.78 HH143 pKa = 6.08 KK144 pKa = 10.75 GRR146 pKa = 11.84 YY147 pKa = 7.55 AA148 pKa = 3.33
Molecular weight: 16.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.155
IPC2_protein 9.136
IPC_protein 9.048
Toseland 9.75
ProMoST 9.487
Dawson 9.999
Bjellqvist 9.692
Wikipedia 10.16
Rodwell 10.379
Grimsley 10.072
Solomon 10.014
Lehninger 9.984
Nozaki 9.823
DTASelect 9.663
Thurlkill 9.838
EMBOSS 10.175
Sillero 9.926
Patrickios 7.571
IPC_peptide 10.014
IPC2_peptide 8.39
IPC2.peptide.svr19 8.117
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
42
0
42
11546
65
1032
274.9
30.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.159 ± 0.357
0.979 ± 0.171
6.253 ± 0.216
6.167 ± 0.268
3.386 ± 0.146
7.327 ± 0.252
2.044 ± 0.175
5.604 ± 0.245
6.643 ± 0.292
8.54 ± 0.292
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.624 ± 0.159
4.954 ± 0.29
3.698 ± 0.177
4.651 ± 0.273
4.833 ± 0.251
5.699 ± 0.327
6.037 ± 0.308
6.773 ± 0.283
1.169 ± 0.104
4.46 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here