Streptomonospora sp. M2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Streptomonospora; unclassified Streptomonospora

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5052 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V0ZJC5|A0A4V0ZJC5_9ACTN Glucosamine kinase GspK OS=Streptomonospora sp. M2 OX=2498135 GN=gspK PE=4 SV=1
MM1 pKa = 7.18NLARR5 pKa = 11.84RR6 pKa = 11.84IALIGGAAALASGFAVALPSEE27 pKa = 4.37PAEE30 pKa = 3.82AHH32 pKa = 6.37GGFTFPATRR41 pKa = 11.84TYY43 pKa = 11.43ACYY46 pKa = 10.32KK47 pKa = 10.54DD48 pKa = 4.53GIEE51 pKa = 4.41GGSGGSLNPQNPMCQQALAEE71 pKa = 4.04NSYY74 pKa = 11.03GFWNWFGNLISDD86 pKa = 3.54AGGRR90 pKa = 11.84HH91 pKa = 5.75RR92 pKa = 11.84EE93 pKa = 4.05IIPDD97 pKa = 3.75GKK99 pKa = 10.87LCGPTEE105 pKa = 4.0QFDD108 pKa = 3.92AFNAPGDD115 pKa = 3.73WPATEE120 pKa = 4.22VQSGDD125 pKa = 3.34TVTFEE130 pKa = 4.24HH131 pKa = 6.49NAWAAHH137 pKa = 6.6PGTFTQYY144 pKa = 8.42ITKK147 pKa = 10.42DD148 pKa = 3.07GWDD151 pKa = 3.56PSQPLGWDD159 pKa = 3.57DD160 pKa = 5.62LEE162 pKa = 4.35PAPFDD167 pKa = 3.98EE168 pKa = 4.74VTNPPKK174 pKa = 10.42RR175 pKa = 11.84SGGVEE180 pKa = 3.53GAEE183 pKa = 4.39YY184 pKa = 10.66YY185 pKa = 9.95WDD187 pKa = 3.26ATLPDD192 pKa = 3.24KK193 pKa = 10.96SGRR196 pKa = 11.84HH197 pKa = 5.2VIYY200 pKa = 10.47SIWQRR205 pKa = 11.84SDD207 pKa = 3.2SPEE210 pKa = 3.71AFYY213 pKa = 11.37NCSDD217 pKa = 4.02VIFGGDD223 pKa = 3.11GGDD226 pKa = 4.31NGNEE230 pKa = 4.87DD231 pKa = 3.88DD232 pKa = 4.48TQAPTAPGAPTADD245 pKa = 3.7SVSGDD250 pKa = 3.52AVDD253 pKa = 5.01LSWAASSDD261 pKa = 3.71NEE263 pKa = 4.07GVTRR267 pKa = 11.84YY268 pKa = 7.72EE269 pKa = 3.86VRR271 pKa = 11.84DD272 pKa = 3.41AATGEE277 pKa = 4.61TVATTSGATSATVGSLDD294 pKa = 3.9PEE296 pKa = 4.14TDD298 pKa = 3.17YY299 pKa = 11.79SFNVVALDD307 pKa = 3.69AAGNASDD314 pKa = 4.6ASPAVSVTTDD324 pKa = 3.04SADD327 pKa = 3.64NTSGACTVDD336 pKa = 3.66FTIANEE342 pKa = 3.84WSGGYY347 pKa = 8.7SANVEE352 pKa = 4.24LTNDD356 pKa = 3.38SDD358 pKa = 4.19SALSSWTVDD367 pKa = 2.78WEE369 pKa = 4.38FSDD372 pKa = 4.5GATVTNAWSADD383 pKa = 3.77VEE385 pKa = 4.25QHH387 pKa = 5.76EE388 pKa = 4.54AHH390 pKa = 6.4VMASNAGWNGSVPAGGSVSFGFNAEE415 pKa = 4.41TTGSAAAPEE424 pKa = 4.38EE425 pKa = 3.97FHH427 pKa = 7.46LDD429 pKa = 3.65GSPCSTAA436 pKa = 3.87

Molecular weight: 45.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V0ZJ76|A0A4V0ZJ76_9ACTN Fructose-1 6-bisphosphatase OS=Streptomonospora sp. M2 OX=2498135 GN=glpX PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVVAARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.55KK38 pKa = 9.79GRR40 pKa = 11.84ARR42 pKa = 11.84LTVSQQ47 pKa = 4.05

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5052

0

5052

1614646

30

4798

319.6

34.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.103 ± 0.066

0.807 ± 0.008

6.128 ± 0.032

6.36 ± 0.034

2.668 ± 0.018

9.749 ± 0.039

2.224 ± 0.016

3.152 ± 0.027

1.569 ± 0.023

9.801 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.853 ± 0.015

1.677 ± 0.018

6.187 ± 0.034

2.681 ± 0.022

8.636 ± 0.038

5.25 ± 0.027

5.475 ± 0.023

8.243 ± 0.038

1.452 ± 0.014

1.984 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski