Aeromonas phage phiAS5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 343 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1A2V4|E1A2V4_9CAUD Uncharacterized protein OS=Aeromonas phage phiAS5 OX=879630 GN=phiAS5_ORF0207 PE=4 SV=1
MM1 pKa = 7.46TNQEE5 pKa = 4.22VIALGLTLVNVIYY18 pKa = 10.39GIGVVSFLEE27 pKa = 3.89IWDD30 pKa = 3.83EE31 pKa = 3.73QDD33 pKa = 3.23TIKK36 pKa = 10.7RR37 pKa = 11.84AIVGLTAMIPFFALSYY53 pKa = 10.78FLLNYY58 pKa = 10.46CEE60 pKa = 4.59VII62 pKa = 3.63

Molecular weight: 6.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1A2T0|E1A2T0_9CAUD Uncharacterized protein OS=Aeromonas phage phiAS5 OX=879630 GN=phiAS5_ORF0183 PE=4 SV=1
MM1 pKa = 7.52NIFKK5 pKa = 10.87RR6 pKa = 11.84LLNRR10 pKa = 11.84MKK12 pKa = 9.73YY13 pKa = 10.57KK14 pKa = 10.8PMIKK18 pKa = 9.57INAYY22 pKa = 10.28AMQIHH27 pKa = 6.06TASGASVCTPDD38 pKa = 5.23GIAVKK43 pKa = 10.39QRR45 pKa = 11.84IEE47 pKa = 3.81RR48 pKa = 11.84LKK50 pKa = 10.92RR51 pKa = 11.84EE52 pKa = 3.94MKK54 pKa = 10.22INRR57 pKa = 11.84GG58 pKa = 3.38

Molecular weight: 6.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

343

0

343

69023

37

1331

201.2

22.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.947 ± 0.166

1.214 ± 0.069

6.428 ± 0.09

6.753 ± 0.162

4.265 ± 0.088

6.449 ± 0.2

1.985 ± 0.07

6.757 ± 0.116

7.316 ± 0.166

6.917 ± 0.12

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.226 ± 0.11

5.358 ± 0.115

3.183 ± 0.081

3.358 ± 0.075

5.066 ± 0.096

6.026 ± 0.121

5.926 ± 0.211

7.379 ± 0.111

1.291 ± 0.052

4.157 ± 0.108
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski