Common moorhen coronavirus HKU21
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H9BR42|H9BR42_9NIDO NS7c protein OS=Common moorhen coronavirus HKU21 OX=1159902 GN=NS7c PE=4 SV=1
MM1 pKa = 7.84 LDD3 pKa = 3.76 FKK5 pKa = 11.21 SAAEE9 pKa = 4.58 SIDD12 pKa = 3.69 SVLLILSQVFNLSIYY27 pKa = 10.78 SEE29 pKa = 4.31 LQGCYY34 pKa = 10.47 YY35 pKa = 10.16 IDD37 pKa = 3.66 GSFWEE42 pKa = 4.23 QDD44 pKa = 3.39 SCDD47 pKa = 3.88 EE48 pKa = 4.23 EE49 pKa = 5.01 SEE51 pKa = 4.09 SDD53 pKa = 3.73 ADD55 pKa = 4.55 ISSDD59 pKa = 3.96 DD60 pKa = 3.4 AA61 pKa = 6.22
Molecular weight: 6.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.47
IPC2_protein 3.147
IPC_protein 3.045
Toseland 2.88
ProMoST 3.3
Dawson 3.096
Bjellqvist 3.274
Wikipedia 3.096
Rodwell 2.918
Grimsley 2.804
Solomon 2.969
Lehninger 2.931
Nozaki 3.261
DTASelect 3.389
Thurlkill 2.982
EMBOSS 3.096
Sillero 3.198
Patrickios 0.006
IPC_peptide 2.956
IPC2_peptide 3.134
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|H9BR41|H9BR41_9NIDO NS7b protein OS=Common moorhen coronavirus HKU21 OX=1159902 GN=NS7b PE=4 SV=1
MM1 pKa = 7.71 GNHH4 pKa = 5.17 QTSIQNTVQNTHH16 pKa = 5.57 IKK18 pKa = 10.41 ASEE21 pKa = 3.99 HH22 pKa = 6.76 SNVSTLTHH30 pKa = 6.51 TFSEE34 pKa = 4.75 LQSVMQIISPVLIIVLSILLIISFALNIALVRR66 pKa = 11.84 KK67 pKa = 9.57 LRR69 pKa = 11.84 FMKK72 pKa = 10.58 AKK74 pKa = 10.07 LRR76 pKa = 11.84 ARR78 pKa = 11.84 TIVKK82 pKa = 9.96 RR83 pKa = 11.84 KK84 pKa = 10.04 SLIDD88 pKa = 3.47 SAA90 pKa = 4.75
Molecular weight: 10.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.189
IPC2_protein 10.014
IPC_protein 11.257
Toseland 11.594
ProMoST 11.915
Dawson 11.608
Bjellqvist 11.491
Wikipedia 11.989
Rodwell 11.74
Grimsley 11.637
Solomon 11.989
Lehninger 11.915
Nozaki 11.579
DTASelect 11.491
Thurlkill 11.579
EMBOSS 12.047
Sillero 11.579
Patrickios 11.506
IPC_peptide 12.003
IPC2_peptide 10.891
IPC2.peptide.svr19 9.157
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
8493
61
6208
943.7
105.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.723 ± 0.735
2.685 ± 0.441
5.722 ± 0.745
3.415 ± 0.23
4.733 ± 0.382
5.204 ± 0.558
2.473 ± 0.388
6.594 ± 0.723
5.216 ± 0.846
8.843 ± 0.612
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.849 ± 0.254
5.958 ± 0.722
4.357 ± 0.779
3.933 ± 0.555
3.356 ± 0.471
6.605 ± 1.365
7.383 ± 0.319
8.89 ± 1.283
0.801 ± 0.184
5.263 ± 0.536
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here