Hubei sobemo-like virus 44

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KET7|A0A1L3KET7_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 44 OX=1923232 PE=4 SV=1
MM1 pKa = 6.73EE2 pKa = 4.54TLRR5 pKa = 11.84RR6 pKa = 11.84AGQALEE12 pKa = 4.24EE13 pKa = 4.51TTSEE17 pKa = 4.21TVNGIKK23 pKa = 10.34SLLGAVSDD31 pKa = 4.66CLEE34 pKa = 4.14EE35 pKa = 4.3FQWPHH40 pKa = 5.85NADD43 pKa = 3.8DD44 pKa = 3.91LTSFRR49 pKa = 11.84IHH51 pKa = 6.45ADD53 pKa = 2.68KK54 pKa = 11.0YY55 pKa = 10.92FRR57 pKa = 11.84LATNACIPDD66 pKa = 4.1LEE68 pKa = 4.27LQEE71 pKa = 4.97RR72 pKa = 11.84VLNKK76 pKa = 9.36TEE78 pKa = 3.35EE79 pKa = 4.2WYY81 pKa = 10.83APVRR85 pKa = 11.84WKK87 pKa = 11.19LPDD90 pKa = 3.36NYY92 pKa = 10.65LSKK95 pKa = 10.98EE96 pKa = 4.02VFKK99 pKa = 10.68TVLRR103 pKa = 11.84RR104 pKa = 11.84LDD106 pKa = 3.94FSSSPGYY113 pKa = 9.37PYY115 pKa = 10.65CKK117 pKa = 9.28RR118 pKa = 11.84KK119 pKa = 8.01QTIGEE124 pKa = 3.98WLGFDD129 pKa = 4.42GFQFDD134 pKa = 4.22PVQVDD139 pKa = 4.51LLWMDD144 pKa = 3.35VQRR147 pKa = 11.84AILGEE152 pKa = 4.18SEE154 pKa = 4.83LIMRR158 pKa = 11.84VFIKK162 pKa = 10.42QEE164 pKa = 3.47PHH166 pKa = 5.9KK167 pKa = 10.44AAKK170 pKa = 9.93AVEE173 pKa = 4.26GRR175 pKa = 11.84WRR177 pKa = 11.84LIMAFPLAHH186 pKa = 5.15QVLWHH191 pKa = 5.86MLFDD195 pKa = 3.9YY196 pKa = 10.95MNDD199 pKa = 3.52LEE201 pKa = 4.32ITKK204 pKa = 10.21AAEE207 pKa = 3.87IPSQQGIVLYY217 pKa = 10.59GGAWKK222 pKa = 10.25SHH224 pKa = 5.29LARR227 pKa = 11.84WKK229 pKa = 9.3QQGYY233 pKa = 8.17DD234 pKa = 3.13TGLDD238 pKa = 3.15KK239 pKa = 11.3SAWDD243 pKa = 3.15WTYY246 pKa = 10.81TYY248 pKa = 11.07WLMQMDD254 pKa = 4.45LQLRR258 pKa = 11.84YY259 pKa = 8.79RR260 pKa = 11.84TGRR263 pKa = 11.84GFKK266 pKa = 9.85MMEE269 pKa = 3.92WFQLATRR276 pKa = 11.84EE277 pKa = 4.03YY278 pKa = 9.29EE279 pKa = 3.94LAFGVGAKK287 pKa = 10.4FITTDD292 pKa = 3.53GYY294 pKa = 11.06EE295 pKa = 4.15LEE297 pKa = 4.15QLVPGLMKK305 pKa = 10.49SGSVNTISSNSHH317 pKa = 4.99AQAMLHH323 pKa = 6.24ALVCIEE329 pKa = 4.74GGVDD333 pKa = 3.53PDD335 pKa = 5.21PYY337 pKa = 10.23PACCGDD343 pKa = 3.68DD344 pKa = 3.67TLQKK348 pKa = 9.85MSQALIPLYY357 pKa = 9.91EE358 pKa = 4.38KK359 pKa = 10.76YY360 pKa = 10.92GVVVKK365 pKa = 10.55SASEE369 pKa = 3.76GMEE372 pKa = 4.49FIGHH376 pKa = 6.57EE377 pKa = 4.34FLDD380 pKa = 4.22SGPQPLYY387 pKa = 9.82MRR389 pKa = 11.84KK390 pKa = 8.66HH391 pKa = 6.0LNRR394 pKa = 11.84FLHH397 pKa = 6.28MKK399 pKa = 10.45DD400 pKa = 3.94EE401 pKa = 4.18YY402 pKa = 11.38LGDD405 pKa = 3.89YY406 pKa = 10.09LDD408 pKa = 4.2GMSRR412 pKa = 11.84LYY414 pKa = 11.16AKK416 pKa = 9.56TPYY419 pKa = 10.29FEE421 pKa = 4.04FWSWLGDD428 pKa = 3.72VYY430 pKa = 11.26DD431 pKa = 4.14VPVPSKK437 pKa = 10.72QYY439 pKa = 9.19VNNWYY444 pKa = 9.77DD445 pKa = 2.94HH446 pKa = 6.8SYY448 pKa = 11.1

Molecular weight: 51.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KET7|A0A1L3KET7_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 44 OX=1923232 PE=4 SV=1
MM1 pKa = 7.82PCFLGLISEE10 pKa = 4.81LSKK13 pKa = 9.98ITAKK17 pKa = 10.38QQTPPTWKK25 pKa = 10.29DD26 pKa = 3.27FLITPSSLGGSTSSKK41 pKa = 9.59IACTVGATVITVGIGVAVVKK61 pKa = 10.18GWYY64 pKa = 8.62RR65 pKa = 11.84LKK67 pKa = 10.4RR68 pKa = 11.84AVGIRR73 pKa = 11.84PSTKK77 pKa = 10.13VQVTPAAVTNHH88 pKa = 5.91GSRR91 pKa = 11.84EE92 pKa = 3.96SRR94 pKa = 11.84FAGSAEE100 pKa = 4.0VAMPMATGQVLIGFYY115 pKa = 10.7SGDD118 pKa = 3.46EE119 pKa = 4.38YY120 pKa = 10.49ITSGGGYY127 pKa = 10.42RR128 pKa = 11.84MDD130 pKa = 4.18LTTGPYY136 pKa = 10.28LVVPCHH142 pKa = 6.52VLDD145 pKa = 3.78EE146 pKa = 4.56VEE148 pKa = 4.18ARR150 pKa = 11.84GSGEE154 pKa = 3.99VVLRR158 pKa = 11.84GPKK161 pKa = 9.24TDD163 pKa = 3.31ISMKK167 pKa = 10.27LDD169 pKa = 3.43RR170 pKa = 11.84EE171 pKa = 4.31NIAEE175 pKa = 4.87IITDD179 pKa = 3.66VYY181 pKa = 10.28VMKK184 pKa = 10.63VQEE187 pKa = 4.23NLFPNLGVKK196 pKa = 9.63KK197 pKa = 9.79PKK199 pKa = 9.52IGRR202 pKa = 11.84IEE204 pKa = 3.83RR205 pKa = 11.84TASAKK210 pKa = 10.09IAGPGGKK217 pKa = 8.2GTLGDD222 pKa = 3.34ISLQIGQVGMTYY234 pKa = 10.69YY235 pKa = 10.76RR236 pKa = 11.84GTTLPGYY243 pKa = 9.48SGCPYY248 pKa = 10.32QVDD251 pKa = 3.96GNIVGMHH258 pKa = 6.02LRR260 pKa = 11.84GSIVPDD266 pKa = 3.86GPNIGIAARR275 pKa = 11.84MIYY278 pKa = 8.4ITANYY283 pKa = 9.87LLAKK287 pKa = 10.33PEE289 pKa = 5.22DD290 pKa = 3.79SLEE293 pKa = 3.98WLTDD297 pKa = 3.53QVIKK301 pKa = 10.66RR302 pKa = 11.84GNKK305 pKa = 9.71LKK307 pKa = 10.06IDD309 pKa = 4.11KK310 pKa = 10.41NWGSLDD316 pKa = 3.37SYY318 pKa = 10.91RR319 pKa = 11.84FQLGGDD325 pKa = 3.91FAIVEE330 pKa = 4.2SDD332 pKa = 3.76TIYY335 pKa = 10.95EE336 pKa = 4.11ALGEE340 pKa = 4.19DD341 pKa = 3.48WLDD344 pKa = 3.4KK345 pKa = 9.1YY346 pKa = 9.38TEE348 pKa = 4.04YY349 pKa = 11.48SDD351 pKa = 5.35FEE353 pKa = 4.39DD354 pKa = 5.04RR355 pKa = 11.84YY356 pKa = 10.34HH357 pKa = 6.65SKK359 pKa = 10.88KK360 pKa = 9.95KK361 pKa = 10.07GKK363 pKa = 10.08SRR365 pKa = 11.84GEE367 pKa = 3.98SAGNEE372 pKa = 3.92QSPGVSGLPEE382 pKa = 4.19SAQGSQSLVGQQSPPSEE399 pKa = 3.99WKK401 pKa = 10.92LSDD404 pKa = 4.0GLDD407 pKa = 3.26KK408 pKa = 11.13LSRR411 pKa = 11.84KK412 pKa = 8.19QRR414 pKa = 11.84QKK416 pKa = 11.34LSTALKK422 pKa = 10.14AFSAQSQTAA431 pKa = 3.0

Molecular weight: 46.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

879

431

448

439.5

49.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.485 ± 0.008

1.138 ± 0.142

5.802 ± 0.474

5.916 ± 0.551

3.299 ± 0.823

9.215 ± 1.62

1.82 ± 0.606

5.006 ± 1.013

6.257 ± 0.478

9.329 ± 0.978

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.958 ± 0.591

2.389 ± 0.047

4.778 ± 0.222

4.664 ± 0.174

4.664 ± 0.016

7.281 ± 1.201

5.461 ± 0.703

6.257 ± 0.478

2.503 ± 0.754

4.778 ± 0.566
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski