Hubei narna-like virus 23

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIF8|A0A1L3KIF8_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 23 OX=1922954 PE=4 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84KK4 pKa = 8.9NVQRR8 pKa = 11.84GSLCKK13 pKa = 10.09LASPCLGSSTPRR25 pKa = 11.84YY26 pKa = 9.26LHH28 pKa = 6.79QLDD31 pKa = 3.95HH32 pKa = 7.01WIKK35 pKa = 10.08HH36 pKa = 6.05HH37 pKa = 6.1GEE39 pKa = 3.5EE40 pKa = 4.62WTCSRR45 pKa = 11.84LKK47 pKa = 10.18TIANAAMQLRR57 pKa = 11.84SGHH60 pKa = 7.15PDD62 pKa = 3.02TARR65 pKa = 11.84EE66 pKa = 3.75ILEE69 pKa = 4.02EE70 pKa = 3.85GRR72 pKa = 11.84IAVRR76 pKa = 11.84KK77 pKa = 9.49DD78 pKa = 3.0KK79 pKa = 10.43PVPRR83 pKa = 11.84GIMGIVALQYY93 pKa = 11.43VNAQKK98 pKa = 10.54PYY100 pKa = 9.73AVRR103 pKa = 11.84TAACLLRR110 pKa = 11.84AYY112 pKa = 10.6SGIYY116 pKa = 9.36LDD118 pKa = 4.07EE119 pKa = 4.65VSVEE123 pKa = 4.05QVRR126 pKa = 11.84KK127 pKa = 9.57ARR129 pKa = 11.84HH130 pKa = 5.75SICDD134 pKa = 3.58KK135 pKa = 10.85AGKK138 pKa = 9.47GQLLPTHH145 pKa = 7.18PGLQWRR151 pKa = 11.84LIHH154 pKa = 6.75EE155 pKa = 4.48GTKK158 pKa = 9.57MSGLVRR164 pKa = 11.84EE165 pKa = 4.75TPRR168 pKa = 11.84SPVDD172 pKa = 3.55LSYY175 pKa = 11.45LNPSRR180 pKa = 11.84KK181 pKa = 9.39YY182 pKa = 9.2FCGQFRR188 pKa = 11.84LAPEE192 pKa = 5.04LKK194 pKa = 10.24DD195 pKa = 3.57KK196 pKa = 10.39PYY198 pKa = 11.23SSAALSLMTMGSVPKK213 pKa = 10.47DD214 pKa = 4.06LITLCGDD221 pKa = 3.95FALRR225 pKa = 11.84RR226 pKa = 11.84QAEE229 pKa = 4.25RR230 pKa = 11.84FQEE233 pKa = 4.21DD234 pKa = 3.33RR235 pKa = 11.84MNYY238 pKa = 9.62PGFLGKK244 pKa = 9.88ISFLQEE250 pKa = 3.95GGCKK254 pKa = 9.76SRR256 pKa = 11.84TVCQPTAWLQAYY268 pKa = 9.12SMPLAAILCRR278 pKa = 11.84AIYY281 pKa = 10.35LLEE284 pKa = 4.19TGKK287 pKa = 10.97GNRR290 pKa = 11.84RR291 pKa = 11.84SLGLSVVHH299 pKa = 6.49DD300 pKa = 3.95QEE302 pKa = 4.51TGSLFMKK309 pKa = 10.4SAMEE313 pKa = 3.7EE314 pKa = 3.79RR315 pKa = 11.84KK316 pKa = 10.34EE317 pKa = 3.89IFSYY321 pKa = 10.6DD322 pKa = 3.53LKK324 pKa = 11.07SATDD328 pKa = 3.76RR329 pKa = 11.84FPRR332 pKa = 11.84GPQLLLLKK340 pKa = 10.77EE341 pKa = 4.14MGLGDD346 pKa = 3.07WCGAVEE352 pKa = 4.35RR353 pKa = 11.84TAEE356 pKa = 4.12GPYY359 pKa = 8.68WAPEE363 pKa = 3.51ARR365 pKa = 11.84QPWSYY370 pKa = 10.05TVGQPMGLAYY380 pKa = 10.58SFSLFHH386 pKa = 6.0LTHH389 pKa = 6.54RR390 pKa = 11.84AILEE394 pKa = 4.2GLAKK398 pKa = 9.91EE399 pKa = 4.21YY400 pKa = 11.0SPNSPAYY407 pKa = 9.78AVLGDD412 pKa = 4.98DD413 pKa = 4.95VLITCKK419 pKa = 10.48SLALAYY425 pKa = 10.19EE426 pKa = 4.25DD427 pKa = 4.62MISGLGVEE435 pKa = 4.57ISTAKK440 pKa = 10.57SFEE443 pKa = 4.3GPDD446 pKa = 3.5LQSFAGFTGISSKK459 pKa = 10.87KK460 pKa = 9.4GITVFRR466 pKa = 11.84PFKK469 pKa = 10.35HH470 pKa = 6.73GPDD473 pKa = 3.62FQIKK477 pKa = 9.51GKK479 pKa = 8.97EE480 pKa = 3.96LNVLHH485 pKa = 6.25TLGPKK490 pKa = 8.75VRR492 pKa = 11.84EE493 pKa = 3.95WSSWWSKK500 pKa = 10.15QFDD503 pKa = 3.73AYY505 pKa = 10.9RR506 pKa = 11.84CTITFRR512 pKa = 11.84GNDD515 pKa = 4.01LSPLLPEE522 pKa = 4.88DD523 pKa = 3.76QPKK526 pKa = 10.55VGPNRR531 pKa = 11.84PSLGWIGSMVNQLTSPGALRR551 pKa = 11.84IKK553 pKa = 10.84GMGGRR558 pKa = 11.84GCVDD562 pKa = 3.18YY563 pKa = 10.65PVDD566 pKa = 3.99YY567 pKa = 10.58FRR569 pKa = 11.84QEE571 pKa = 3.7HH572 pKa = 5.75NRR574 pKa = 11.84LFLGRR579 pKa = 11.84LSNWFNQKK587 pKa = 8.67AWNHH591 pKa = 5.41EE592 pKa = 3.75FSILVSDD599 pKa = 5.13KK600 pKa = 10.77EE601 pKa = 4.42PNSPAIFAPEE611 pKa = 3.72LLKK614 pKa = 10.9RR615 pKa = 11.84EE616 pKa = 4.58DD617 pKa = 3.98GPFVRR622 pKa = 11.84PYY624 pKa = 10.63SALSTDD630 pKa = 4.1PLIQGHH636 pKa = 5.84RR637 pKa = 11.84HH638 pKa = 4.94DD639 pKa = 3.53QYY641 pKa = 11.39LRR643 pKa = 11.84KK644 pKa = 9.72LRR646 pKa = 11.84EE647 pKa = 3.73EE648 pKa = 3.9SRR650 pKa = 11.84EE651 pKa = 4.15SIRR654 pKa = 11.84KK655 pKa = 8.49QVEE658 pKa = 3.62QEE660 pKa = 3.55ALIRR664 pKa = 11.84AKK666 pKa = 9.92EE667 pKa = 3.86EE668 pKa = 3.55KK669 pKa = 10.01LARR672 pKa = 11.84EE673 pKa = 3.81EE674 pKa = 5.12LEE676 pKa = 3.91RR677 pKa = 11.84QGCLEE682 pKa = 3.8RR683 pKa = 11.84EE684 pKa = 3.4RR685 pKa = 11.84RR686 pKa = 11.84ARR688 pKa = 11.84IKK690 pKa = 11.05APVTKK695 pKa = 8.76PTSRR699 pKa = 11.84STGLGRR705 pKa = 4.33

Molecular weight: 79.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIF8|A0A1L3KIF8_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 23 OX=1922954 PE=4 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84KK4 pKa = 8.9NVQRR8 pKa = 11.84GSLCKK13 pKa = 10.09LASPCLGSSTPRR25 pKa = 11.84YY26 pKa = 9.26LHH28 pKa = 6.79QLDD31 pKa = 3.95HH32 pKa = 7.01WIKK35 pKa = 10.08HH36 pKa = 6.05HH37 pKa = 6.1GEE39 pKa = 3.5EE40 pKa = 4.62WTCSRR45 pKa = 11.84LKK47 pKa = 10.18TIANAAMQLRR57 pKa = 11.84SGHH60 pKa = 7.15PDD62 pKa = 3.02TARR65 pKa = 11.84EE66 pKa = 3.75ILEE69 pKa = 4.02EE70 pKa = 3.85GRR72 pKa = 11.84IAVRR76 pKa = 11.84KK77 pKa = 9.49DD78 pKa = 3.0KK79 pKa = 10.43PVPRR83 pKa = 11.84GIMGIVALQYY93 pKa = 11.43VNAQKK98 pKa = 10.54PYY100 pKa = 9.73AVRR103 pKa = 11.84TAACLLRR110 pKa = 11.84AYY112 pKa = 10.6SGIYY116 pKa = 9.36LDD118 pKa = 4.07EE119 pKa = 4.65VSVEE123 pKa = 4.05QVRR126 pKa = 11.84KK127 pKa = 9.57ARR129 pKa = 11.84HH130 pKa = 5.75SICDD134 pKa = 3.58KK135 pKa = 10.85AGKK138 pKa = 9.47GQLLPTHH145 pKa = 7.18PGLQWRR151 pKa = 11.84LIHH154 pKa = 6.75EE155 pKa = 4.48GTKK158 pKa = 9.57MSGLVRR164 pKa = 11.84EE165 pKa = 4.75TPRR168 pKa = 11.84SPVDD172 pKa = 3.55LSYY175 pKa = 11.45LNPSRR180 pKa = 11.84KK181 pKa = 9.39YY182 pKa = 9.2FCGQFRR188 pKa = 11.84LAPEE192 pKa = 5.04LKK194 pKa = 10.24DD195 pKa = 3.57KK196 pKa = 10.39PYY198 pKa = 11.23SSAALSLMTMGSVPKK213 pKa = 10.47DD214 pKa = 4.06LITLCGDD221 pKa = 3.95FALRR225 pKa = 11.84RR226 pKa = 11.84QAEE229 pKa = 4.25RR230 pKa = 11.84FQEE233 pKa = 4.21DD234 pKa = 3.33RR235 pKa = 11.84MNYY238 pKa = 9.62PGFLGKK244 pKa = 9.88ISFLQEE250 pKa = 3.95GGCKK254 pKa = 9.76SRR256 pKa = 11.84TVCQPTAWLQAYY268 pKa = 9.12SMPLAAILCRR278 pKa = 11.84AIYY281 pKa = 10.35LLEE284 pKa = 4.19TGKK287 pKa = 10.97GNRR290 pKa = 11.84RR291 pKa = 11.84SLGLSVVHH299 pKa = 6.49DD300 pKa = 3.95QEE302 pKa = 4.51TGSLFMKK309 pKa = 10.4SAMEE313 pKa = 3.7EE314 pKa = 3.79RR315 pKa = 11.84KK316 pKa = 10.34EE317 pKa = 3.89IFSYY321 pKa = 10.6DD322 pKa = 3.53LKK324 pKa = 11.07SATDD328 pKa = 3.76RR329 pKa = 11.84FPRR332 pKa = 11.84GPQLLLLKK340 pKa = 10.77EE341 pKa = 4.14MGLGDD346 pKa = 3.07WCGAVEE352 pKa = 4.35RR353 pKa = 11.84TAEE356 pKa = 4.12GPYY359 pKa = 8.68WAPEE363 pKa = 3.51ARR365 pKa = 11.84QPWSYY370 pKa = 10.05TVGQPMGLAYY380 pKa = 10.58SFSLFHH386 pKa = 6.0LTHH389 pKa = 6.54RR390 pKa = 11.84AILEE394 pKa = 4.2GLAKK398 pKa = 9.91EE399 pKa = 4.21YY400 pKa = 11.0SPNSPAYY407 pKa = 9.78AVLGDD412 pKa = 4.98DD413 pKa = 4.95VLITCKK419 pKa = 10.48SLALAYY425 pKa = 10.19EE426 pKa = 4.25DD427 pKa = 4.62MISGLGVEE435 pKa = 4.57ISTAKK440 pKa = 10.57SFEE443 pKa = 4.3GPDD446 pKa = 3.5LQSFAGFTGISSKK459 pKa = 10.87KK460 pKa = 9.4GITVFRR466 pKa = 11.84PFKK469 pKa = 10.35HH470 pKa = 6.73GPDD473 pKa = 3.62FQIKK477 pKa = 9.51GKK479 pKa = 8.97EE480 pKa = 3.96LNVLHH485 pKa = 6.25TLGPKK490 pKa = 8.75VRR492 pKa = 11.84EE493 pKa = 3.95WSSWWSKK500 pKa = 10.15QFDD503 pKa = 3.73AYY505 pKa = 10.9RR506 pKa = 11.84CTITFRR512 pKa = 11.84GNDD515 pKa = 4.01LSPLLPEE522 pKa = 4.88DD523 pKa = 3.76QPKK526 pKa = 10.55VGPNRR531 pKa = 11.84PSLGWIGSMVNQLTSPGALRR551 pKa = 11.84IKK553 pKa = 10.84GMGGRR558 pKa = 11.84GCVDD562 pKa = 3.18YY563 pKa = 10.65PVDD566 pKa = 3.99YY567 pKa = 10.58FRR569 pKa = 11.84QEE571 pKa = 3.7HH572 pKa = 5.75NRR574 pKa = 11.84LFLGRR579 pKa = 11.84LSNWFNQKK587 pKa = 8.67AWNHH591 pKa = 5.41EE592 pKa = 3.75FSILVSDD599 pKa = 5.13KK600 pKa = 10.77EE601 pKa = 4.42PNSPAIFAPEE611 pKa = 3.72LLKK614 pKa = 10.9RR615 pKa = 11.84EE616 pKa = 4.58DD617 pKa = 3.98GPFVRR622 pKa = 11.84PYY624 pKa = 10.63SALSTDD630 pKa = 4.1PLIQGHH636 pKa = 5.84RR637 pKa = 11.84HH638 pKa = 4.94DD639 pKa = 3.53QYY641 pKa = 11.39LRR643 pKa = 11.84KK644 pKa = 9.72LRR646 pKa = 11.84EE647 pKa = 3.73EE648 pKa = 3.9SRR650 pKa = 11.84EE651 pKa = 4.15SIRR654 pKa = 11.84KK655 pKa = 8.49QVEE658 pKa = 3.62QEE660 pKa = 3.55ALIRR664 pKa = 11.84AKK666 pKa = 9.92EE667 pKa = 3.86EE668 pKa = 3.55KK669 pKa = 10.01LARR672 pKa = 11.84EE673 pKa = 3.81EE674 pKa = 5.12LEE676 pKa = 3.91RR677 pKa = 11.84QGCLEE682 pKa = 3.8RR683 pKa = 11.84EE684 pKa = 3.4RR685 pKa = 11.84RR686 pKa = 11.84ARR688 pKa = 11.84IKK690 pKa = 11.05APVTKK695 pKa = 8.76PTSRR699 pKa = 11.84STGLGRR705 pKa = 4.33

Molecular weight: 79.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

705

705

705

705.0

79.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.234 ± 0.0

2.128 ± 0.0

3.972 ± 0.0

6.667 ± 0.0

3.546 ± 0.0

8.085 ± 0.0

2.411 ± 0.0

4.255 ± 0.0

6.099 ± 0.0

10.922 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.0

2.27 ± 0.0

6.241 ± 0.0

4.255 ± 0.0

8.227 ± 0.0

7.801 ± 0.0

4.255 ± 0.0

4.397 ± 0.0

1.844 ± 0.0

3.262 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski