Cymbidium ringspot virus satellite RNA
Average proteome isoelectric point is 9.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q66228|Q66228_9VIRU Uncharacterized protein OS=Cymbidium ringspot virus satellite RNA OX=192023 PE=4 SV=1
MM1 pKa = 7.36 LLSVRR6 pKa = 11.84 KK7 pKa = 9.8 DD8 pKa = 2.76 RR9 pKa = 11.84 WFLTTNGGHH18 pKa = 6.37 AAKK21 pKa = 10.09 GLLGIRR27 pKa = 11.84 TAEE30 pKa = 4.1 VTVCLPPYY38 pKa = 10.0 CVRR41 pKa = 4.28
Molecular weight: 4.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.205
IPC2_protein 9.107
IPC_protein 9.355
Toseland 9.853
ProMoST 10.101
Dawson 10.058
Bjellqvist 9.838
Wikipedia 10.218
Rodwell 10.335
Grimsley 10.101
Solomon 10.189
Lehninger 10.175
Nozaki 10.072
DTASelect 9.765
Thurlkill 9.926
EMBOSS 10.262
Sillero 10.043
Patrickios 10.423
IPC_peptide 10.189
IPC2_peptide 9.18
IPC2.peptide.svr19 8.036
Protein with the highest isoelectric point:
>tr|Q66228|Q66228_9VIRU Uncharacterized protein OS=Cymbidium ringspot virus satellite RNA OX=192023 PE=4 SV=1
MM1 pKa = 7.36 LLSVRR6 pKa = 11.84 KK7 pKa = 9.8 DD8 pKa = 2.76 RR9 pKa = 11.84 WFLTTNGGHH18 pKa = 6.37 AAKK21 pKa = 10.09 GLLGIRR27 pKa = 11.84 TAEE30 pKa = 4.1 VTVCLPPYY38 pKa = 10.0 CVRR41 pKa = 4.28
Molecular weight: 4.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.205
IPC2_protein 9.107
IPC_protein 9.355
Toseland 9.853
ProMoST 10.101
Dawson 10.058
Bjellqvist 9.838
Wikipedia 10.218
Rodwell 10.335
Grimsley 10.101
Solomon 10.189
Lehninger 10.175
Nozaki 10.072
DTASelect 9.765
Thurlkill 9.926
EMBOSS 10.262
Sillero 10.043
Patrickios 10.423
IPC_peptide 10.189
IPC2_peptide 9.18
IPC2.peptide.svr19 8.036
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
41
41
41
41.0
4.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.317 ± 0.0
4.878 ± 0.0
2.439 ± 0.0
2.439 ± 0.0
2.439 ± 0.0
9.756 ± 0.0
2.439 ± 0.0
2.439 ± 0.0
4.878 ± 0.0
14.634 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.439 ± 0.0
2.439 ± 0.0
4.878 ± 0.0
0.0 ± 0.0
9.756 ± 0.0
2.439 ± 0.0
9.756 ± 0.0
9.756 ± 0.0
2.439 ± 0.0
2.439 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here