Wenzhou bivalvia virus 2
Average proteome isoelectric point is 7.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KL44|A0A1L3KL44_9VIRU Uncharacterized protein OS=Wenzhou bivalvia virus 2 OX=1923554 PE=4 SV=1
MM1 pKa = 7.63 TDD3 pKa = 3.17 AKK5 pKa = 10.5 VKK7 pKa = 10.51 RR8 pKa = 11.84 PAGRR12 pKa = 11.84 QCNNWQPVSHH22 pKa = 6.55 PRR24 pKa = 11.84 KK25 pKa = 10.01 GKK27 pKa = 8.94 GHH29 pKa = 5.27 VANRR33 pKa = 11.84 SNSGLSRR40 pKa = 11.84 FYY42 pKa = 11.09 CGIKK46 pKa = 10.48 GEE48 pKa = 4.24 NEE50 pKa = 3.39 ANIAVEE56 pKa = 4.64 TISLLDD62 pKa = 4.52 QINLDD67 pKa = 3.22 WKK69 pKa = 10.83 SVVKK73 pKa = 10.45 RR74 pKa = 11.84 HH75 pKa = 6.04 ALPDD79 pKa = 4.54 GYY81 pKa = 9.98 PCALVAFAACLPFEE95 pKa = 3.97 EE96 pKa = 5.95 HH97 pKa = 6.62 IEE99 pKa = 4.02 YY100 pKa = 9.91 FYY102 pKa = 10.78 KK103 pKa = 10.57 QLMLNGLLARR113 pKa = 11.84 NEE115 pKa = 3.99 QGPPGGMSLVGMLKK129 pKa = 10.48 ALNTWEE135 pKa = 3.75 QAVRR139 pKa = 11.84 NKK141 pKa = 10.35 RR142 pKa = 11.84 FRR144 pKa = 11.84 TDD146 pKa = 2.68 KK147 pKa = 10.85 LRR149 pKa = 11.84 GMDD152 pKa = 3.4 VHH154 pKa = 7.08 HH155 pKa = 6.78 ITLKK159 pKa = 10.03 GSKK162 pKa = 9.61 IVDD165 pKa = 3.89 FSIYY169 pKa = 8.62 PTGLKK174 pKa = 10.12 GSNVEE179 pKa = 4.12 TIIIFIEE186 pKa = 4.24 GDD188 pKa = 3.09 GRR190 pKa = 11.84 EE191 pKa = 4.12 GHH193 pKa = 6.23 FFSANKK199 pKa = 8.2 MWPGQHH205 pKa = 6.01 YY206 pKa = 10.46 RR207 pKa = 11.84 MKK209 pKa = 10.62 QLAEE213 pKa = 3.8 QNRR216 pKa = 11.84 LIEE219 pKa = 4.3 EE220 pKa = 4.53 AAQTTSNVPTPSSEE234 pKa = 3.72 PRR236 pKa = 11.84 VTVTTPEE243 pKa = 3.84 SRR245 pKa = 11.84 DD246 pKa = 3.29 IEE248 pKa = 4.6 TVTWLGLTPDD258 pKa = 3.69 EE259 pKa = 4.55 LNIVEE264 pKa = 4.59 ASVGDD269 pKa = 3.87 MDD271 pKa = 5.33 PEE273 pKa = 4.46 PEE275 pKa = 3.87 EE276 pKa = 4.11 SRR278 pKa = 11.84 EE279 pKa = 4.0 EE280 pKa = 3.86 IAGQGIMIEE289 pKa = 4.32 PYY291 pKa = 10.4 EE292 pKa = 4.37 PYY294 pKa = 10.18 HH295 pKa = 7.32 LNGKK299 pKa = 8.92 VINTASDD306 pKa = 3.98 DD307 pKa = 4.22 LPSLIPGQPAMPRR320 pKa = 11.84 PRR322 pKa = 11.84 VGRR325 pKa = 11.84 RR326 pKa = 11.84 LSYY329 pKa = 10.92 DD330 pKa = 2.56 GTMVVWSPDD339 pKa = 3.5 PPDD342 pKa = 4.55 PPSPPPEE349 pKa = 4.53 EE350 pKa = 4.16 IRR352 pKa = 11.84 PPSPPPSPMLEE363 pKa = 3.98 FVPDD367 pKa = 3.53 EE368 pKa = 4.44 SIEE371 pKa = 4.44 FIKK374 pKa = 10.4 TNVTVPRR381 pKa = 11.84 FTHH384 pKa = 6.74 ALRR387 pKa = 11.84 GYY389 pKa = 10.74 AVGDD393 pKa = 4.09 LLPDD397 pKa = 3.9 GAVGHH402 pKa = 6.82 TDD404 pKa = 3.68 ASCEE408 pKa = 4.12 NACGATEE415 pKa = 3.88 LHH417 pKa = 6.81 PAVQEE422 pKa = 4.06 KK423 pKa = 10.62 FGFTRR428 pKa = 11.84 RR429 pKa = 11.84 SLYY432 pKa = 10.24 YY433 pKa = 9.2 VSADD437 pKa = 3.09 AGVNPLDD444 pKa = 4.83 AKK446 pKa = 11.07 YY447 pKa = 8.86 LTNGQYY453 pKa = 10.53 NPAWLTSMVFGNTSYY468 pKa = 10.83 VLEE471 pKa = 4.19 YY472 pKa = 11.0 VGIFSDD478 pKa = 3.71 CHH480 pKa = 5.47 FFRR483 pKa = 11.84 LAEE486 pKa = 4.01 TATIVPKK493 pKa = 10.4 SRR495 pKa = 11.84 KK496 pKa = 8.69 FFSRR500 pKa = 11.84 LFSGLGAPAVGTLVCKK516 pKa = 10.37 GAALLKK522 pKa = 10.12 VLGVAASVGAVGTVSGPIIATAGLAYY548 pKa = 10.15 AAFPDD553 pKa = 4.05 AVPTLTHH560 pKa = 6.51 RR561 pKa = 11.84 LVGVLRR567 pKa = 11.84 EE568 pKa = 3.82 RR569 pKa = 11.84 GFISEE574 pKa = 4.89 LPAKK578 pKa = 10.08 CFHH581 pKa = 6.4 AQKK584 pKa = 10.46 KK585 pKa = 9.81 LPAADD590 pKa = 4.3 LSTFDD595 pKa = 3.19 GHH597 pKa = 6.9 RR598 pKa = 11.84 RR599 pKa = 11.84 AEE601 pKa = 4.04 WTVMRR606 pKa = 11.84 QYY608 pKa = 10.91 VRR610 pKa = 11.84 EE611 pKa = 4.01 DD612 pKa = 3.44 LVPAFHH618 pKa = 7.13 SLQVRR623 pKa = 11.84 NGDD626 pKa = 3.44 HH627 pKa = 7.65 DD628 pKa = 4.56 VDD630 pKa = 4.02 PEE632 pKa = 3.99 MALEE636 pKa = 4.27 GLQTLKK642 pKa = 10.75 HH643 pKa = 5.91 RR644 pKa = 11.84 LEE646 pKa = 4.38 TKK648 pKa = 10.27 RR649 pKa = 11.84 GTRR652 pKa = 11.84 GYY654 pKa = 10.98 KK655 pKa = 9.61 VFQTSGPKK663 pKa = 9.32 HH664 pKa = 6.52 CKK666 pKa = 9.85 SCGKK670 pKa = 9.09 PPPPAGVKK678 pKa = 9.39 YY679 pKa = 9.05 KK680 pKa = 9.71 WKK682 pKa = 10.4 HH683 pKa = 4.98 RR684 pKa = 11.84 VCTDD688 pKa = 3.17 CDD690 pKa = 3.54 KK691 pKa = 11.27 TLGLCGAITPMGRR704 pKa = 11.84 DD705 pKa = 3.05 IQANCAVADD714 pKa = 4.35 GPPGRR719 pKa = 11.84 VHH721 pKa = 6.93 MYY723 pKa = 10.78 SSTLPPKK730 pKa = 9.69 KK731 pKa = 10.3 KK732 pKa = 9.53 KK733 pKa = 8.87 WEE735 pKa = 4.03 QVEE738 pKa = 4.47 VPPGAITIRR747 pKa = 11.84 ASDD750 pKa = 4.48 APWMAGVRR758 pKa = 11.84 TMAKK762 pKa = 9.2 VLEE765 pKa = 4.31 VTKK768 pKa = 10.9 EE769 pKa = 4.09 DD770 pKa = 3.11 IFKK773 pKa = 10.26 IDD775 pKa = 3.03 TSLEE779 pKa = 3.84 KK780 pKa = 10.38 RR781 pKa = 11.84 RR782 pKa = 11.84 QEE784 pKa = 3.99 CVLAGIAISGCYY796 pKa = 9.85 PMVTQKK802 pKa = 11.27 GLYY805 pKa = 10.2 SRR807 pKa = 11.84 MQALLGRR814 pKa = 11.84 AFLKK818 pKa = 10.57 KK819 pKa = 9.85 PQSCPRR825 pKa = 11.84 AWKK828 pKa = 10.47 KK829 pKa = 10.17 MEE831 pKa = 4.67 DD832 pKa = 3.95 LKK834 pKa = 11.49 HH835 pKa = 6.6 LILPKK840 pKa = 10.5 GALDD844 pKa = 4.26 GPQMDD849 pKa = 3.79 VEE851 pKa = 4.35 EE852 pKa = 5.58 WIASMPGRR860 pKa = 11.84 RR861 pKa = 11.84 KK862 pKa = 9.71 RR863 pKa = 11.84 ALKK866 pKa = 10.07 RR867 pKa = 11.84 AYY869 pKa = 9.23 KK870 pKa = 10.32 QFMEE874 pKa = 5.7 DD875 pKa = 3.67 GLILEE880 pKa = 4.86 KK881 pKa = 10.94 DD882 pKa = 3.53 LTFSAFVKK890 pKa = 10.66 QEE892 pKa = 3.63 LLAAFEE898 pKa = 4.59 EE899 pKa = 5.03 YY900 pKa = 10.19 EE901 pKa = 4.47 GPVSKK906 pKa = 10.52 EE907 pKa = 3.74 LEE909 pKa = 3.91 EE910 pKa = 4.44 TIARR914 pKa = 11.84 MIMAPQDD921 pKa = 3.6 KK922 pKa = 9.85 AHH924 pKa = 6.62 VIAGPVIKK932 pKa = 9.99 PKK934 pKa = 10.73 LMRR937 pKa = 11.84 LKK939 pKa = 10.72 DD940 pKa = 3.5 HH941 pKa = 6.32 WHH943 pKa = 6.17 HH944 pKa = 7.2 DD945 pKa = 2.81 NWLFYY950 pKa = 11.1 GATTPKK956 pKa = 10.49 NLQSWLDD963 pKa = 3.63 KK964 pKa = 11.21 AVGICADD971 pKa = 3.78 GEE973 pKa = 4.8 VFTFWCDD980 pKa = 3.48 FSMFDD985 pKa = 4.04 CTHH988 pKa = 6.46 NEE990 pKa = 3.67 YY991 pKa = 11.29 SMKK994 pKa = 10.53 LIEE997 pKa = 4.56 SYY999 pKa = 10.27 YY1000 pKa = 10.63 SEE1002 pKa = 4.82 MGTSPLFKK1010 pKa = 10.02 MIIDD1014 pKa = 4.1 AWRR1017 pKa = 11.84 VPAGTMGEE1025 pKa = 4.24 LKK1027 pKa = 10.5 FRR1029 pKa = 11.84 LHH1031 pKa = 7.04 QIMLASGRR1039 pKa = 11.84 DD1040 pKa = 3.41 DD1041 pKa = 3.63 TALMNAMYY1049 pKa = 10.02 CGFVMGLAVAAAVRR1063 pKa = 11.84 NKK1065 pKa = 10.11 PLEE1068 pKa = 4.49 DD1069 pKa = 4.33 LDD1071 pKa = 4.77 SGDD1074 pKa = 3.89 ILFAAAYY1081 pKa = 9.44 VRR1083 pKa = 11.84 ISICGDD1089 pKa = 3.13 DD1090 pKa = 3.78 TLGFLPKK1097 pKa = 10.43 NLWFRR1102 pKa = 11.84 RR1103 pKa = 11.84 AQIMASIEE1111 pKa = 4.31 TNLSRR1116 pKa = 11.84 FGLVSKK1122 pKa = 10.45 LDD1124 pKa = 3.56 CSNYY1128 pKa = 10.15 LGSAVYY1134 pKa = 10.57 LGMRR1138 pKa = 11.84 PYY1140 pKa = 10.75 NVPTPFGRR1148 pKa = 11.84 QWLWGRR1154 pKa = 11.84 TIGRR1158 pKa = 11.84 AAYY1161 pKa = 9.69 KK1162 pKa = 9.2 MGWMLDD1168 pKa = 3.64 LSKK1171 pKa = 11.4 GDD1173 pKa = 3.67 AAAWAYY1179 pKa = 10.22 GVADD1183 pKa = 5.56 AIARR1187 pKa = 11.84 TQPYY1191 pKa = 9.79 VPLLSDD1197 pKa = 3.97 LAKK1200 pKa = 10.53 KK1201 pKa = 10.23 VVEE1204 pKa = 4.39 LSQGYY1209 pKa = 9.45 KK1210 pKa = 8.6 RR1211 pKa = 11.84 TPVLEE1216 pKa = 4.83 DD1217 pKa = 3.7 PNKK1220 pKa = 10.24 PWTHH1224 pKa = 5.29 WTPHH1228 pKa = 5.72 EE1229 pKa = 4.16 NLGQLTYY1236 pKa = 11.16 DD1237 pKa = 4.0 DD1238 pKa = 4.04 QTLEE1242 pKa = 4.34 CLVLSYY1248 pKa = 7.98 DD1249 pKa = 3.87 TPTHH1253 pKa = 6.46 HH1254 pKa = 7.08 GASQPVSPTIHH1265 pKa = 7.05 DD1266 pKa = 3.47 LHH1268 pKa = 7.66 RR1269 pKa = 11.84 SVRR1272 pKa = 11.84 NIQKK1276 pKa = 9.6 IDD1278 pKa = 3.5 RR1279 pKa = 11.84 LPYY1282 pKa = 9.98 NLEE1285 pKa = 4.45 DD1286 pKa = 3.81 YY1287 pKa = 11.18 ALQCYY1292 pKa = 9.44 CNRR1295 pKa = 11.84 DD1296 pKa = 3.4 DD1297 pKa = 4.91 KK1298 pKa = 11.86
Molecular weight: 144.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.942
IPC2_protein 7.322
IPC_protein 7.132
Toseland 6.912
ProMoST 7.79
Dawson 7.834
Bjellqvist 8.258
Wikipedia 7.746
Rodwell 7.863
Grimsley 7.015
Solomon 7.849
Lehninger 7.878
Nozaki 8.478
DTASelect 7.995
Thurlkill 8.053
EMBOSS 8.053
Sillero 8.361
Patrickios 4.317
IPC_peptide 7.849
IPC2_peptide 7.337
IPC2.peptide.svr19 7.289
Protein with the highest isoelectric point:
>tr|A0A1L3KL44|A0A1L3KL44_9VIRU Uncharacterized protein OS=Wenzhou bivalvia virus 2 OX=1923554 PE=4 SV=1
MM1 pKa = 7.34 TFLSAVLLPVRR12 pKa = 11.84 AKK14 pKa = 10.43 EE15 pKa = 3.63 RR16 pKa = 11.84 TARR19 pKa = 11.84 VMSHH23 pKa = 6.56 TGLKK27 pKa = 10.59 SLDD30 pKa = 3.37 QVAQTICLPNEE41 pKa = 4.01 RR42 pKa = 11.84 APVRR46 pKa = 11.84 LPTYY50 pKa = 10.14 PSIDD54 pKa = 3.03 KK55 pKa = 9.25 TALFRR60 pKa = 11.84 YY61 pKa = 9.49 RR62 pKa = 11.84 YY63 pKa = 10.09 QNTEE67 pKa = 3.71 SLKK70 pKa = 10.6 DD71 pKa = 3.87 DD72 pKa = 3.94 KK73 pKa = 11.13 LVPPGDD79 pKa = 4.76 LDD81 pKa = 3.71 TLNIPGRR88 pKa = 11.84 KK89 pKa = 8.84 RR90 pKa = 11.84 FILSRR95 pKa = 11.84 DD96 pKa = 3.12 PAAPMLLDD104 pKa = 3.87 SVHH107 pKa = 6.89 LLQHH111 pKa = 5.65 TWGLDD116 pKa = 3.02 PGAFDD121 pKa = 3.22 GTIFIRR127 pKa = 11.84 GEE129 pKa = 3.87 TVYY132 pKa = 10.98 LVDD135 pKa = 3.98 SASGPSTVHH144 pKa = 6.52 SVDD147 pKa = 3.8 FDD149 pKa = 3.18 KK150 pKa = 11.1 CYY152 pKa = 10.1 PIEE155 pKa = 4.1 YY156 pKa = 10.07 YY157 pKa = 8.98 RR158 pKa = 11.84 TLPAGRR164 pKa = 11.84 LDD166 pKa = 3.5 GKK168 pKa = 9.76 EE169 pKa = 3.5 WFMVPRR175 pKa = 11.84 VLDD178 pKa = 3.55 SRR180 pKa = 11.84 GKK182 pKa = 9.38 SQPFLNVLCVGLITVDD198 pKa = 4.11 GPWPFAANPLGKK210 pKa = 10.44 GLIRR214 pKa = 11.84 TYY216 pKa = 10.87 HH217 pKa = 6.36 SDD219 pKa = 3.64 ASVTSLNASNGEE231 pKa = 3.87 LCLDD235 pKa = 3.45 YY236 pKa = 10.77 TITIEE241 pKa = 4.74 AMSGDD246 pKa = 3.71 GAVQEE251 pKa = 5.26 LVMSYY256 pKa = 10.8 DD257 pKa = 3.08 WSNWDD262 pKa = 3.33 YY263 pKa = 11.17 TGSFDD268 pKa = 4.91 LHH270 pKa = 6.84 PDD272 pKa = 3.03 VAMVRR277 pKa = 11.84 IKK279 pKa = 10.83 SLTYY283 pKa = 9.29 RR284 pKa = 11.84 GLVRR288 pKa = 11.84 LVDD291 pKa = 3.92 NNLPGADD298 pKa = 3.47 VPMKK302 pKa = 10.46 GCYY305 pKa = 8.71 PVLGLSRR312 pKa = 11.84 SGAHH316 pKa = 6.22 IPAGSPPTTFRR327 pKa = 11.84 CLSLPPTAVNPEE339 pKa = 4.36 YY340 pKa = 10.21 YY341 pKa = 10.6 NSVAPFQSTRR351 pKa = 11.84 LNSSAVLLTNVSKK364 pKa = 11.05 VLNKK368 pKa = 10.29 EE369 pKa = 3.84 GTVQSSRR376 pKa = 11.84 LLFNPNAGQTVHH388 pKa = 6.62 HH389 pKa = 7.25 ADD391 pKa = 3.38 VVSVSTSNPDD401 pKa = 2.8 TRR403 pKa = 11.84 YY404 pKa = 10.23 FGALEE409 pKa = 3.95 KK410 pKa = 10.7 GAYY413 pKa = 8.23 TFTAPDD419 pKa = 3.42 QEE421 pKa = 4.44 SLKK424 pKa = 10.79 FVTPYY429 pKa = 9.55 VTVDD433 pKa = 3.23 VNDD436 pKa = 4.04 AGTGEE441 pKa = 4.24 GNIAILTLVSTKK453 pKa = 10.74 NVVRR457 pKa = 11.84 PVLDD461 pKa = 4.68 LGAKK465 pKa = 9.44 YY466 pKa = 9.79 YY467 pKa = 11.2 NCIICTDD474 pKa = 4.35 LDD476 pKa = 4.05 STDD479 pKa = 3.75 DD480 pKa = 3.65 TQLALTLDD488 pKa = 3.78 THH490 pKa = 5.87 WEE492 pKa = 3.82 FRR494 pKa = 11.84 TISTLYY500 pKa = 8.6 TLDD503 pKa = 3.39 YY504 pKa = 11.04 SRR506 pKa = 11.84 IPMEE510 pKa = 4.32 VYY512 pKa = 9.64 HH513 pKa = 6.86 AAMLAVVKK521 pKa = 10.88 AGFFYY526 pKa = 10.94 EE527 pKa = 4.38 NNNHH531 pKa = 6.14 AAILRR536 pKa = 11.84 MLSAGVKK543 pKa = 9.79 FAAPLIAGYY552 pKa = 9.69 AARR555 pKa = 11.84 IPAGFLQAQATSALAKK571 pKa = 10.22 GAVNLALHH579 pKa = 6.38 GAKK582 pKa = 9.69 KK583 pKa = 10.08 AHH585 pKa = 6.21 AAYY588 pKa = 7.75 RR589 pKa = 11.84 THH591 pKa = 6.69 QNNKK595 pKa = 8.54 QKK597 pKa = 10.57 QKK599 pKa = 10.4 PKK601 pKa = 10.16 NKK603 pKa = 9.16 EE604 pKa = 3.75 RR605 pKa = 11.84 GNMRR609 pKa = 11.84 QKK611 pKa = 11.12 GLRR614 pKa = 3.51
Molecular weight: 67.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.733
IPC2_protein 7.98
IPC_protein 7.849
Toseland 7.746
ProMoST 8.448
Dawson 8.58
Bjellqvist 8.77
Wikipedia 8.58
Rodwell 8.609
Grimsley 7.79
Solomon 8.668
Lehninger 8.653
Nozaki 8.843
DTASelect 8.58
Thurlkill 8.624
EMBOSS 8.756
Sillero 8.887
Patrickios 4.228
IPC_peptide 8.653
IPC2_peptide 7.644
IPC2.peptide.svr19 7.828
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1912
614
1298
956.0
105.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.682 ± 0.257
1.831 ± 0.309
5.701 ± 0.191
4.759 ± 1.071
3.504 ± 0.049
7.27 ± 0.347
2.615 ± 0.101
4.184 ± 0.352
5.596 ± 0.512
9.623 ± 0.755
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.772 ± 0.479
3.661 ± 0.718
6.956 ± 0.354
2.981 ± 0.124
5.596 ± 0.13
6.015 ± 0.579
6.119 ± 0.804
6.904 ± 0.536
1.621 ± 0.377
3.609 ± 0.367
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here