Amino acid dipepetide frequency for Novosphingobium sp. 9U

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.254AlaAla: 18.254 ± 0.19	1.254AlaCys: 1.254 ± 0.034	7.103AlaAsp: 7.103 ± 0.075	7.507AlaGlu: 7.507 ± 0.081	4.221AlaPhe: 4.221 ± 0.063	11.164AlaGly: 11.164 ± 0.111	2.45AlaHis: 2.45 ± 0.051	6.347AlaIle: 6.347 ± 0.074	4.101AlaLys: 4.101 ± 0.067	13.783AlaLeu: 13.783 ± 0.142	3.773AlaMet: 3.773 ± 0.059	3.041AlaAsn: 3.041 ± 0.049	6.403AlaPro: 6.403 ± 0.094	4.967AlaGln: 4.967 ± 0.078	9.67AlaArg: 9.67 ± 0.098	7.097AlaSer: 7.097 ± 0.076	6.42AlaThr: 6.42 ± 0.075	8.643AlaVal: 8.643 ± 0.078	1.728AlaTrp: 1.728 ± 0.04	2.599AlaTyr: 2.599 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	1.13CysAla: 1.13 ± 0.035	0.132CysCys: 0.132 ± 0.01	0.595CysAsp: 0.595 ± 0.022	0.536CysGlu: 0.536 ± 0.02	0.319CysPhe: 0.319 ± 0.014	0.957CysGly: 0.957 ± 0.028	0.251CysHis: 0.251 ± 0.013	0.357CysIle: 0.357 ± 0.016	0.228CysLys: 0.228 ± 0.014	0.82CysLeu: 0.82 ± 0.026	0.207CysMet: 0.207 ± 0.014	0.239CysAsn: 0.239 ± 0.015	0.516CysPro: 0.516 ± 0.021	0.262CysGln: 0.262 ± 0.012	0.681CysArg: 0.681 ± 0.025	0.567CysSer: 0.567 ± 0.022	0.446CysThr: 0.446 ± 0.019	0.645CysVal: 0.645 ± 0.019	0.119CysTrp: 0.119 ± 0.009	0.195CysTyr: 0.195 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.393AspAla: 7.393 ± 0.075	0.544AspCys: 0.544 ± 0.018	3.096AspAsp: 3.096 ± 0.054	3.401AspGlu: 3.401 ± 0.061	2.078AspPhe: 2.078 ± 0.045	5.081AspGly: 5.081 ± 0.066	1.291AspHis: 1.291 ± 0.036	2.5AspIle: 2.5 ± 0.045	1.84AspLys: 1.84 ± 0.04	5.643AspLeu: 5.643 ± 0.066	1.31AspMet: 1.31 ± 0.031	1.342AspAsn: 1.342 ± 0.03	3.648AspPro: 3.648 ± 0.055	1.778AspGln: 1.778 ± 0.034	4.489AspArg: 4.489 ± 0.062	2.15AspSer: 2.15 ± 0.041	2.713AspThr: 2.713 ± 0.044	4.167AspVal: 4.167 ± 0.062	1.064AspTrp: 1.064 ± 0.03	1.489AspTyr: 1.489 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	7.796GluAla: 7.796 ± 0.088	0.393GluCys: 0.393 ± 0.016	2.802GluAsp: 2.802 ± 0.052	2.962GluGlu: 2.962 ± 0.053	1.609GluPhe: 1.609 ± 0.037	4.558GluGly: 4.558 ± 0.061	1.389GluHis: 1.389 ± 0.033	2.801GluIle: 2.801 ± 0.046	1.763GluLys: 1.763 ± 0.04	5.348GluLeu: 5.348 ± 0.064	1.317GluMet: 1.317 ± 0.032	1.309GluAsn: 1.309 ± 0.027	2.879GluPro: 2.879 ± 0.047	2.283GluGln: 2.283 ± 0.044	5.156GluArg: 5.156 ± 0.075	2.268GluSer: 2.268 ± 0.037	2.919GluThr: 2.919 ± 0.043	3.831GluVal: 3.831 ± 0.049	0.861GluTrp: 0.861 ± 0.026	1.067GluTyr: 1.067 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	4.784PheAla: 4.784 ± 0.057	0.37PheCys: 0.37 ± 0.016	2.53PheAsp: 2.53 ± 0.04	2.009PheGlu: 2.009 ± 0.039	1.202PhePhe: 1.202 ± 0.035	3.685PheGly: 3.685 ± 0.061	0.735PheHis: 0.735 ± 0.024	1.3PheIle: 1.3 ± 0.034	0.862PheLys: 0.862 ± 0.025	2.951PheLeu: 2.951 ± 0.048	0.671PheMet: 0.671 ± 0.024	0.994PheAsn: 0.994 ± 0.028	1.437PhePro: 1.437 ± 0.032	0.943PheGln: 0.943 ± 0.027	2.122PheArg: 2.122 ± 0.04	2.006PheSer: 2.006 ± 0.044	1.914PheThr: 1.914 ± 0.04	2.666PheVal: 2.666 ± 0.045	0.521PheTrp: 0.521 ± 0.021	0.926PheTyr: 0.926 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	9.879GlyAla: 9.879 ± 0.093	0.927GlyCys: 0.927 ± 0.028	4.586GlyAsp: 4.586 ± 0.061	4.998GlyGlu: 4.998 ± 0.064	3.404GlyPhe: 3.404 ± 0.057	7.908GlyGly: 7.908 ± 0.111	1.794GlyHis: 1.794 ± 0.038	4.124GlyIle: 4.124 ± 0.055	3.311GlyLys: 3.311 ± 0.06	8.634GlyLeu: 8.634 ± 0.091	2.404GlyMet: 2.404 ± 0.043	2.206GlyAsn: 2.206 ± 0.04	3.69GlyPro: 3.69 ± 0.055	3.1GlyGln: 3.1 ± 0.05	6.226GlyArg: 6.226 ± 0.068	5.19GlySer: 5.19 ± 0.072	5.139GlyThr: 5.139 ± 0.08	6.4GlyVal: 6.4 ± 0.079	1.595GlyTrp: 1.595 ± 0.04	2.417GlyTyr: 2.417 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	2.617HisAla: 2.617 ± 0.042	0.254HisCys: 0.254 ± 0.015	1.237HisAsp: 1.237 ± 0.032	1.139HisGlu: 1.139 ± 0.029	0.84HisPhe: 0.84 ± 0.022	2.072HisGly: 2.072 ± 0.043	0.608HisHis: 0.608 ± 0.026	0.834HisIle: 0.834 ± 0.024	0.496HisLys: 0.496 ± 0.02	1.994HisLeu: 1.994 ± 0.044	0.451HisMet: 0.451 ± 0.02	0.473HisAsn: 0.473 ± 0.018	1.324HisPro: 1.324 ± 0.037	0.62HisGln: 0.62 ± 0.019	1.563HisArg: 1.563 ± 0.04	1.044HisSer: 1.044 ± 0.027	0.824HisThr: 0.824 ± 0.023	1.569HisVal: 1.569 ± 0.037	0.332HisTrp: 0.332 ± 0.017	0.567HisTyr: 0.567 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	7.129IleAla: 7.129 ± 0.08	0.474IleCys: 0.474 ± 0.018	3.626IleAsp: 3.626 ± 0.048	3.39IleGlu: 3.39 ± 0.044	1.252IlePhe: 1.252 ± 0.031	4.74IleGly: 4.74 ± 0.061	0.882IleHis: 0.882 ± 0.023	1.656IleIle: 1.656 ± 0.039	1.249IleLys: 1.249 ± 0.03	3.557IleLeu: 3.557 ± 0.062	0.879IleMet: 0.879 ± 0.027	1.301IleAsn: 1.301 ± 0.035	2.082IlePro: 2.082 ± 0.041	1.118IleGln: 1.118 ± 0.03	2.889IleArg: 2.889 ± 0.045	2.565IleSer: 2.565 ± 0.044	2.408IleThr: 2.408 ± 0.044	3.873IleVal: 3.873 ± 0.055	0.551IleTrp: 0.551 ± 0.023	0.992IleTyr: 0.992 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	3.951LysAla: 3.951 ± 0.064	0.179LysCys: 0.179 ± 0.012	1.49LysAsp: 1.49 ± 0.037	1.238LysGlu: 1.238 ± 0.037	0.855LysPhe: 0.855 ± 0.027	2.529LysGly: 2.529 ± 0.045	0.561LysHis: 0.561 ± 0.023	1.405LysIle: 1.405 ± 0.035	0.947LysLys: 0.947 ± 0.03	3.364LysLeu: 3.364 ± 0.055	0.7LysMet: 0.7 ± 0.024	0.678LysAsn: 0.678 ± 0.022	2.068LysPro: 2.068 ± 0.039	1.046LysGln: 1.046 ± 0.029	2.321LysArg: 2.321 ± 0.041	1.661LysSer: 1.661 ± 0.032	1.589LysThr: 1.589 ± 0.039	2.377LysVal: 2.377 ± 0.045	0.368LysTrp: 0.368 ± 0.018	0.613LysTyr: 0.613 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	14.532LeuAla: 14.532 ± 0.125	0.879LeuCys: 0.879 ± 0.023	6.028LeuAsp: 6.028 ± 0.07	4.996LeuGlu: 4.996 ± 0.061	3.371LeuPhe: 3.371 ± 0.054	8.591LeuGly: 8.591 ± 0.079	1.922LeuHis: 1.922 ± 0.04	4.372LeuIle: 4.372 ± 0.073	2.903LeuLys: 2.903 ± 0.05	9.484LeuLeu: 9.484 ± 0.114	2.062LeuMet: 2.062 ± 0.04	2.38LeuAsn: 2.38 ± 0.043	5.675LeuPro: 5.675 ± 0.068	2.935LeuGln: 2.935 ± 0.048	7.061LeuArg: 7.061 ± 0.077	6.079LeuSer: 6.079 ± 0.064	5.636LeuThr: 5.636 ± 0.064	7.541LeuVal: 7.541 ± 0.087	1.197LeuTrp: 1.197 ± 0.039	1.891LeuTyr: 1.891 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	3.133MetAla: 3.133 ± 0.049	0.172MetCys: 0.172 ± 0.011	1.132MetAsp: 1.132 ± 0.026	1.035MetGlu: 1.035 ± 0.029	0.686MetPhe: 0.686 ± 0.024	1.819MetGly: 1.819 ± 0.04	0.47MetHis: 0.47 ± 0.02	1.287MetIle: 1.287 ± 0.035	0.85MetLys: 0.85 ± 0.025	2.575MetLeu: 2.575 ± 0.051	0.619MetMet: 0.619 ± 0.023	0.684MetAsn: 0.684 ± 0.022	1.495MetPro: 1.495 ± 0.035	0.791MetGln: 0.791 ± 0.026	1.836MetArg: 1.836 ± 0.034	1.541MetSer: 1.541 ± 0.032	1.689MetThr: 1.689 ± 0.035	1.632MetVal: 1.632 ± 0.038	0.23MetTrp: 0.23 ± 0.013	0.287MetTyr: 0.287 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.292AsnAla: 3.292 ± 0.052	0.294AsnCys: 0.294 ± 0.016	1.355AsnAsp: 1.355 ± 0.028	1.137AsnGlu: 1.137 ± 0.033	0.938AsnPhe: 0.938 ± 0.031	2.396AsnGly: 2.396 ± 0.046	0.482AsnHis: 0.482 ± 0.019	1.1AsnIle: 1.1 ± 0.033	0.67AsnLys: 0.67 ± 0.024	2.554AsnLeu: 2.554 ± 0.051	0.537AsnMet: 0.537 ± 0.018	0.702AsnAsn: 0.702 ± 0.028	1.709AsnPro: 1.709 ± 0.039	0.753AsnGln: 0.753 ± 0.025	1.801AsnArg: 1.801 ± 0.039	1.264AsnSer: 1.264 ± 0.036	1.218AsnThr: 1.218 ± 0.034	1.894AsnVal: 1.894 ± 0.038	0.413AsnTrp: 0.413 ± 0.02	0.714AsnTyr: 0.714 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	6.939ProAla: 6.939 ± 0.091	0.396ProCys: 0.396 ± 0.017	3.469ProAsp: 3.469 ± 0.06	3.776ProGlu: 3.776 ± 0.054	1.862ProPhe: 1.862 ± 0.044	4.698ProGly: 4.698 ± 0.053	1.07ProHis: 1.07 ± 0.028	2.421ProIle: 2.421 ± 0.041	1.508ProLys: 1.508 ± 0.035	5.18ProLeu: 5.18 ± 0.067	1.192ProMet: 1.192 ± 0.034	1.314ProAsn: 1.314 ± 0.031	2.679ProPro: 2.679 ± 0.062	1.929ProGln: 1.929 ± 0.039	3.287ProArg: 3.287 ± 0.057	3.035ProSer: 3.035 ± 0.049	2.681ProThr: 2.681 ± 0.049	4.2ProVal: 4.2 ± 0.057	0.727ProTrp: 0.727 ± 0.023	1.085ProTyr: 1.085 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.689GlnAla: 4.689 ± 0.063	0.246GlnCys: 0.246 ± 0.011	1.717GlnAsp: 1.717 ± 0.036	1.491GlnGlu: 1.491 ± 0.034	1.148GlnPhe: 1.148 ± 0.028	2.772GlnGly: 2.772 ± 0.043	0.699GlnHis: 0.699 ± 0.025	1.705GlnIle: 1.705 ± 0.037	0.906GlnLys: 0.906 ± 0.029	3.397GlnLeu: 3.397 ± 0.049	0.84GlnMet: 0.84 ± 0.023	0.78GlnAsn: 0.78 ± 0.026	1.89GlnPro: 1.89 ± 0.044	1.271GlnGln: 1.271 ± 0.035	2.601GlnArg: 2.601 ± 0.051	1.797GlnSer: 1.797 ± 0.033	1.705GlnThr: 1.705 ± 0.038	2.755GlnVal: 2.755 ± 0.051	0.486GlnTrp: 0.486 ± 0.021	0.634GlnTyr: 0.634 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	8.392ArgAla: 8.392 ± 0.097	0.665ArgCys: 0.665 ± 0.025	4.083ArgAsp: 4.083 ± 0.051	4.332ArgGlu: 4.332 ± 0.058	3.023ArgPhe: 3.023 ± 0.051	5.115ArgGly: 5.115 ± 0.069	1.799ArgHis: 1.799 ± 0.04	3.88ArgIle: 3.88 ± 0.055	2.271ArgLys: 2.271 ± 0.038	7.966ArgLeu: 7.966 ± 0.088	1.975ArgMet: 1.975 ± 0.039	1.899ArgAsn: 1.899 ± 0.035	3.643ArgPro: 3.643 ± 0.051	2.663ArgGln: 2.663 ± 0.046	6.042ArgArg: 6.042 ± 0.089	4.133ArgSer: 4.133 ± 0.061	3.664ArgThr: 3.664 ± 0.051	4.995ArgVal: 4.995 ± 0.062	1.227ArgTrp: 1.227 ± 0.03	1.921ArgTyr: 1.921 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	6.819SerAla: 6.819 ± 0.08	0.465SerCys: 0.465 ± 0.017	3.088SerAsp: 3.088 ± 0.048	2.838SerGlu: 2.838 ± 0.048	2.12SerPhe: 2.12 ± 0.039	5.712SerGly: 5.712 ± 0.075	1.116SerHis: 1.116 ± 0.03	2.504SerIle: 2.504 ± 0.046	1.517SerLys: 1.517 ± 0.033	5.416SerLeu: 5.416 ± 0.067	1.346SerMet: 1.346 ± 0.032	1.473SerAsn: 1.473 ± 0.033	2.997SerPro: 2.997 ± 0.047	1.827SerGln: 1.827 ± 0.04	3.842SerArg: 3.842 ± 0.062	3.289SerSer: 3.289 ± 0.062	2.926SerThr: 2.926 ± 0.059	3.831SerVal: 3.831 ± 0.053	0.822SerTrp: 0.822 ± 0.026	1.468SerTyr: 1.468 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	6.324ThrAla: 6.324 ± 0.074	0.509ThrCys: 0.509 ± 0.017	2.739ThrAsp: 2.739 ± 0.05	2.333ThrGlu: 2.333 ± 0.043	1.934ThrPhe: 1.934 ± 0.035	5.38ThrGly: 5.38 ± 0.078	1.024ThrHis: 1.024 ± 0.029	2.876ThrIle: 2.876 ± 0.051	1.398ThrLys: 1.398 ± 0.034	5.647ThrLeu: 5.647 ± 0.066	1.272ThrMet: 1.272 ± 0.032	1.288ThrAsn: 1.288 ± 0.033	3.328ThrPro: 3.328 ± 0.057	1.685ThrGln: 1.685 ± 0.036	3.61ThrArg: 3.61 ± 0.053	3.055ThrSer: 3.055 ± 0.058	2.811ThrThr: 2.811 ± 0.053	4.033ThrVal: 4.033 ± 0.055	0.747ThrTrp: 0.747 ± 0.026	1.247ThrTyr: 1.247 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	9.424ValAla: 9.424 ± 0.095	0.629ValCys: 0.629 ± 0.02	4.141ValAsp: 4.141 ± 0.055	4.322ValGlu: 4.322 ± 0.05	2.355ValPhe: 2.355 ± 0.044	5.718ValGly: 5.718 ± 0.077	1.439ValHis: 1.439 ± 0.033	3.657ValIle: 3.657 ± 0.06	1.979ValLys: 1.979 ± 0.042	7.267ValLeu: 7.267 ± 0.073	1.611ValMet: 1.611 ± 0.036	2.0ValAsn: 2.0 ± 0.042	4.136ValPro: 4.136 ± 0.055	2.166ValGln: 2.166 ± 0.041	5.271ValArg: 5.271 ± 0.06	4.465ValSer: 4.465 ± 0.064	4.592ValThr: 4.592 ± 0.059	5.48ValVal: 5.48 ± 0.067	0.909ValTrp: 0.909 ± 0.025	1.4ValTyr: 1.4 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.368TrpAla: 1.368 ± 0.033	0.157TrpCys: 0.157 ± 0.009	0.711TrpAsp: 0.711 ± 0.026	0.627TrpGlu: 0.627 ± 0.024	0.585TrpPhe: 0.585 ± 0.021	0.923TrpGly: 0.923 ± 0.03	0.392TrpHis: 0.392 ± 0.016	0.674TrpIle: 0.674 ± 0.022	0.48TrpLys: 0.48 ± 0.02	1.804TrpLeu: 1.804 ± 0.047	0.379TrpMet: 0.379 ± 0.016	0.483TrpAsn: 0.483 ± 0.019	0.712TrpPro: 0.712 ± 0.022	0.648TrpGln: 0.648 ± 0.021	1.382TrpArg: 1.382 ± 0.032	0.952TrpSer: 0.952 ± 0.03	0.809TrpThr: 0.809 ± 0.026	0.856TrpVal: 0.856 ± 0.026	0.242TrpTrp: 0.242 ± 0.014	0.314TrpTyr: 0.314 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.682TyrAla: 2.682 ± 0.047	0.264TyrCys: 0.264 ± 0.015	1.488TyrAsp: 1.488 ± 0.032	1.241TyrGlu: 1.241 ± 0.03	0.829TyrPhe: 0.829 ± 0.025	2.116TyrGly: 2.116 ± 0.047	0.485TyrHis: 0.485 ± 0.019	0.813TyrIle: 0.813 ± 0.025	0.661TyrLys: 0.661 ± 0.026	2.148TyrLeu: 2.148 ± 0.048	0.414TyrMet: 0.414 ± 0.018	0.635TyrAsn: 0.635 ± 0.021	1.141TyrPro: 1.141 ± 0.031	0.744TyrGln: 0.744 ± 0.02	1.84TyrArg: 1.84 ± 0.039	1.257TyrSer: 1.257 ± 0.03	1.138TyrThr: 1.138 ± 0.033	1.572TyrVal: 1.572 ± 0.037	0.361TyrTrp: 0.361 ± 0.017	0.644TyrTyr: 0.644 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4782 proteins (1383869 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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