Siphoviridae sp. cttb18

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WBE0|A0A5Q2WBE0_9CAUD Uncharacterized protein OS=Siphoviridae sp. cttb18 OX=2656723 PE=4 SV=1
MM1 pKa = 7.59ASYY4 pKa = 11.39NKK6 pKa = 9.41FQPFVEE12 pKa = 4.27KK13 pKa = 10.52LAEE16 pKa = 4.19KK17 pKa = 10.31AHH19 pKa = 6.06NLGSDD24 pKa = 3.62TLTVALTTAANAPVATNGVLADD46 pKa = 4.28LTQIAYY52 pKa = 8.22TNLSSRR58 pKa = 11.84AITTTTSSQSSGTYY72 pKa = 10.44KK73 pKa = 10.69LVLADD78 pKa = 4.67LVLTASGAVATFRR91 pKa = 11.84YY92 pKa = 9.27VVIYY96 pKa = 10.85NDD98 pKa = 3.27TATNDD103 pKa = 3.94EE104 pKa = 4.62LIGWYY109 pKa = 9.85DD110 pKa = 3.38YY111 pKa = 11.72GDD113 pKa = 3.66NVTLANGDD121 pKa = 3.92TFTLDD126 pKa = 3.56FDD128 pKa = 3.96ATNGFIQIAA137 pKa = 3.54

Molecular weight: 14.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WBD1|A0A5Q2WBD1_9CAUD Uncharacterized protein OS=Siphoviridae sp. cttb18 OX=2656723 PE=4 SV=1
MM1 pKa = 7.5LFKK4 pKa = 10.76FGIDD8 pKa = 3.36LFVSKK13 pKa = 10.75VLIRR17 pKa = 11.84IIYY20 pKa = 7.54FSRR23 pKa = 11.84MFSFYY28 pKa = 10.47FVFPIPIVLEE38 pKa = 3.86NEE40 pKa = 4.4YY41 pKa = 10.87ACRR44 pKa = 11.84WMHH47 pKa = 6.85LPISKK52 pKa = 10.42SLFGKK57 pKa = 10.05MMDD60 pKa = 3.52KK61 pKa = 10.28ILRR64 pKa = 11.84FFYY67 pKa = 10.48RR68 pKa = 11.84VGKK71 pKa = 8.54LTHH74 pKa = 6.63LLVIKK79 pKa = 10.0QCISACVKK87 pKa = 10.67SNCQSRR93 pKa = 11.84NSRR96 pKa = 11.84SLNHH100 pKa = 6.5HH101 pKa = 6.14LLFRR105 pKa = 11.84VLYY108 pKa = 10.09LGNQSTITTLNHH120 pKa = 5.91YY121 pKa = 8.6FLSIIILFFLIKK133 pKa = 10.36PFLLKK138 pKa = 9.96TRR140 pKa = 11.84FKK142 pKa = 10.86ISINFLLKK150 pKa = 10.53VFQCGKK156 pKa = 9.24FQWSSCSFF164 pKa = 3.57

Molecular weight: 19.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

12773

29

1014

177.4

20.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.13 ± 0.3

1.112 ± 0.144

5.645 ± 0.24

7.242 ± 0.312

4.705 ± 0.225

6.459 ± 0.353

1.879 ± 0.189

6.866 ± 0.232

9.035 ± 0.405

8.604 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.771 ± 0.198

4.744 ± 0.222

3.664 ± 0.16

4.306 ± 0.236

4.345 ± 0.217

5.418 ± 0.247

5.527 ± 0.323

6.224 ± 0.242

1.746 ± 0.162

3.578 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski