Pseudoalteromonas phage H105/1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|E1AC18|E1AC18_9CAUD Conserved hypothetical phage protein OS=Pseudoalteromonas phage H105/1 OX=877240 PE=4 SV=1
MM1 pKa = 6.88TTLKK5 pKa = 10.89SEE7 pKa = 4.29FQEE10 pKa = 4.28LAAEE14 pKa = 4.95LIDD17 pKa = 6.07DD18 pKa = 4.71EE19 pKa = 5.07FADD22 pKa = 4.4FQRR25 pKa = 11.84PFIIKK30 pKa = 10.36KK31 pKa = 9.81NGDD34 pKa = 3.51YY35 pKa = 11.22SPVTGEE41 pKa = 4.09SPSFNAEE48 pKa = 3.55TGAIPLDD55 pKa = 3.62LKK57 pKa = 9.8TAEE60 pKa = 4.3KK61 pKa = 10.86VFTNVTSSEE70 pKa = 4.07IYY72 pKa = 10.64LVILNAAPVPSDD84 pKa = 3.16FDD86 pKa = 3.78EE87 pKa = 5.58SYY89 pKa = 8.98HH90 pKa = 6.44CSYY93 pKa = 11.39NGVDD97 pKa = 3.44YY98 pKa = 10.86DD99 pKa = 3.83INQVEE104 pKa = 4.86GDD106 pKa = 4.05PADD109 pKa = 3.04AAYY112 pKa = 9.61FVRR115 pKa = 11.84IVII118 pKa = 4.24

Molecular weight:
13.07 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|E1ABZ6|E1ABZ6_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage H105/1 OX=877240 PE=4 SV=1
MM1 pKa = 7.23NAGAGVIEE9 pKa = 4.39CQYY12 pKa = 10.94LYY14 pKa = 10.97LILYY18 pKa = 8.79PMYY21 pKa = 10.93LITNYY26 pKa = 10.37LWLITLAMVSFLTLRR41 pKa = 11.84CRR43 pKa = 11.84AISLRR48 pKa = 11.84DD49 pKa = 3.8AITEE53 pKa = 3.91KK54 pKa = 11.16GLIKK58 pKa = 10.47ALSLIMRR65 pKa = 11.84RR66 pKa = 11.84FSGYY70 pKa = 8.22RR71 pKa = 11.84TLRR74 pKa = 11.84LKK76 pKa = 10.82ALAKK80 pKa = 10.01RR81 pKa = 11.84LPFFACNN88 pKa = 3.21

Molecular weight:
10.16 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

9503

33

866

182.8

20.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.524 ± 0.652

1.084 ± 0.159

6.577 ± 0.308

6.577 ± 0.453

4.051 ± 0.251

7.345 ± 0.461

0.958 ± 0.146

6.608 ± 0.299

6.64 ± 0.503

8.197 ± 0.446

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.252 ± 0.29

5.64 ± 0.296

2.673 ± 0.268

4.335 ± 0.407

4.136 ± 0.347

7.766 ± 0.425

6.198 ± 0.368

6.072 ± 0.308

0.8 ± 0.117

3.567 ± 0.236

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski