Pseudoalteromonas phage H105/1
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E1AC18|E1AC18_9CAUD Conserved hypothetical phage protein OS=Pseudoalteromonas phage H105/1 OX=877240 PE=4 SV=1
MM1 pKa = 6.88 TTLKK5 pKa = 10.89 SEE7 pKa = 4.29 FQEE10 pKa = 4.28 LAAEE14 pKa = 4.95 LIDD17 pKa = 6.07 DD18 pKa = 4.71 EE19 pKa = 5.07 FADD22 pKa = 4.4 FQRR25 pKa = 11.84 PFIIKK30 pKa = 10.36 KK31 pKa = 9.81 NGDD34 pKa = 3.51 YY35 pKa = 11.22 SPVTGEE41 pKa = 4.09 SPSFNAEE48 pKa = 3.55 TGAIPLDD55 pKa = 3.62 LKK57 pKa = 9.8 TAEE60 pKa = 4.3 KK61 pKa = 10.86 VFTNVTSSEE70 pKa = 4.07 IYY72 pKa = 10.64 LVILNAAPVPSDD84 pKa = 3.16 FDD86 pKa = 3.78 EE87 pKa = 5.58 SYY89 pKa = 8.98 HH90 pKa = 6.44 CSYY93 pKa = 11.39 NGVDD97 pKa = 3.44 YY98 pKa = 10.86 DD99 pKa = 3.83 INQVEE104 pKa = 4.86 GDD106 pKa = 4.05 PADD109 pKa = 3.04 AAYY112 pKa = 9.61 FVRR115 pKa = 11.84 IVII118 pKa = 4.24
Molecular weight: 13.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.986
IPC2_protein 3.961
IPC_protein 3.91
Toseland 3.706
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.732
Grimsley 3.617
Solomon 3.872
Lehninger 3.821
Nozaki 3.999
DTASelect 4.19
Thurlkill 3.757
EMBOSS 3.821
Sillero 4.024
Patrickios 2.88
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.921
Protein with the highest isoelectric point:
>tr|E1ABZ6|E1ABZ6_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage H105/1 OX=877240 PE=4 SV=1
MM1 pKa = 7.23 NAGAGVIEE9 pKa = 4.39 CQYY12 pKa = 10.94 LYY14 pKa = 10.97 LILYY18 pKa = 8.79 PMYY21 pKa = 10.93 LITNYY26 pKa = 10.37 LWLITLAMVSFLTLRR41 pKa = 11.84 CRR43 pKa = 11.84 AISLRR48 pKa = 11.84 DD49 pKa = 3.8 AITEE53 pKa = 3.91 KK54 pKa = 11.16 GLIKK58 pKa = 10.47 ALSLIMRR65 pKa = 11.84 RR66 pKa = 11.84 FSGYY70 pKa = 8.22 RR71 pKa = 11.84 TLRR74 pKa = 11.84 LKK76 pKa = 10.82 ALAKK80 pKa = 10.01 RR81 pKa = 11.84 LPFFACNN88 pKa = 3.21
Molecular weight: 10.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.109
IPC2_protein 9.721
IPC_protein 10.218
Toseland 9.926
ProMoST 9.838
Dawson 10.248
Bjellqvist 10.028
Wikipedia 10.482
Rodwell 10.409
Grimsley 10.35
Solomon 10.292
Lehninger 10.233
Nozaki 10.028
DTASelect 9.999
Thurlkill 10.058
EMBOSS 10.365
Sillero 10.175
Patrickios 7.439
IPC_peptide 10.277
IPC2_peptide 9.224
IPC2.peptide.svr19 8.395
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
52
0
52
9503
33
866
182.8
20.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.524 ± 0.652
1.084 ± 0.159
6.577 ± 0.308
6.577 ± 0.453
4.051 ± 0.251
7.345 ± 0.461
0.958 ± 0.146
6.608 ± 0.299
6.64 ± 0.503
8.197 ± 0.446
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.252 ± 0.29
5.64 ± 0.296
2.673 ± 0.268
4.335 ± 0.407
4.136 ± 0.347
7.766 ± 0.425
6.198 ± 0.368
6.072 ± 0.308
0.8 ± 0.117
3.567 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here