Amino acid dipepetide frequency for Caenorhabditis japonica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.153AlaAla: 6.153 ± 0.048	1.164AlaCys: 1.164 ± 0.011	3.463AlaAsp: 3.463 ± 0.022	4.474AlaGlu: 4.474 ± 0.041	2.755AlaPhe: 2.755 ± 0.019	3.496AlaGly: 3.496 ± 0.028	1.553AlaHis: 1.553 ± 0.012	3.919AlaIle: 3.919 ± 0.023	4.199AlaLys: 4.199 ± 0.027	5.729AlaLeu: 5.729 ± 0.031	1.659AlaMet: 1.659 ± 0.013	2.983AlaAsn: 2.983 ± 0.019	3.822AlaPro: 3.822 ± 0.04	2.837AlaGln: 2.837 ± 0.025	3.715AlaArg: 3.715 ± 0.028	5.101AlaSer: 5.101 ± 0.03	4.238AlaThr: 4.238 ± 0.028	4.596AlaVal: 4.596 ± 0.027	0.726AlaTrp: 0.726 ± 0.008	1.707AlaTyr: 1.707 ± 0.014	0.013AlaXaa: 0.013 ± 0.001
Cys	1.258CysAla: 1.258 ± 0.014	0.51CysCys: 0.51 ± 0.013	1.06CysAsp: 1.06 ± 0.015	1.23CysGlu: 1.23 ± 0.014	0.88CysPhe: 0.88 ± 0.01	1.27CysGly: 1.27 ± 0.016	0.517CysHis: 0.517 ± 0.009	0.98CysIle: 0.98 ± 0.013	0.96CysLys: 0.96 ± 0.013	1.576CysLeu: 1.576 ± 0.015	0.417CysMet: 0.417 ± 0.007	0.776CysAsn: 0.776 ± 0.011	1.134CysPro: 1.134 ± 0.017	0.923CysGln: 0.923 ± 0.018	1.114CysArg: 1.114 ± 0.011	1.646CysSer: 1.646 ± 0.018	1.069CysThr: 1.069 ± 0.011	1.223CysVal: 1.223 ± 0.014	0.21CysTrp: 0.21 ± 0.005	0.597CysTyr: 0.597 ± 0.011	0.007CysXaa: 0.007 ± 0.001
Asp	3.473AspAla: 3.473 ± 0.029	0.936AspCys: 0.936 ± 0.013	3.966AspAsp: 3.966 ± 0.03	4.714AspGlu: 4.714 ± 0.039	2.389AspPhe: 2.389 ± 0.016	3.587AspGly: 3.587 ± 0.046	1.14AspHis: 1.14 ± 0.012	2.894AspIle: 2.894 ± 0.017	2.823AspLys: 2.823 ± 0.019	4.348AspLeu: 4.348 ± 0.022	1.242AspMet: 1.242 ± 0.013	2.175AspAsn: 2.175 ± 0.016	2.391AspPro: 2.391 ± 0.017	1.86AspGln: 1.86 ± 0.014	2.86AspArg: 2.86 ± 0.02	3.835AspSer: 3.835 ± 0.022	2.604AspThr: 2.604 ± 0.016	3.744AspVal: 3.744 ± 0.021	0.715AspTrp: 0.715 ± 0.009	1.689AspTyr: 1.689 ± 0.014	0.01AspXaa: 0.01 ± 0.001
Glu	4.268GluAla: 4.268 ± 0.03	1.109GluCys: 1.109 ± 0.015	4.118GluAsp: 4.118 ± 0.032	6.656GluGlu: 6.656 ± 0.049	2.459GluPhe: 2.459 ± 0.018	2.689GluGly: 2.689 ± 0.017	1.504GluHis: 1.504 ± 0.016	3.822GluIle: 3.822 ± 0.022	5.991GluLys: 5.991 ± 0.037	5.543GluLeu: 5.543 ± 0.03	1.982GluMet: 1.982 ± 0.015	3.676GluAsn: 3.676 ± 0.025	2.601GluPro: 2.601 ± 0.026	2.911GluGln: 2.911 ± 0.021	3.818GluArg: 3.818 ± 0.028	4.386GluSer: 4.386 ± 0.026	3.778GluThr: 3.778 ± 0.031	3.857GluVal: 3.857 ± 0.024	0.866GluTrp: 0.866 ± 0.01	1.824GluTyr: 1.824 ± 0.016	0.015GluXaa: 0.015 ± 0.001
Phe	3.035PheAla: 3.035 ± 0.019	0.927PheCys: 0.927 ± 0.012	2.543PheAsp: 2.543 ± 0.017	2.672PheGlu: 2.672 ± 0.018	2.154PhePhe: 2.154 ± 0.019	2.844PheGly: 2.844 ± 0.025	1.079PheHis: 1.079 ± 0.011	2.307PheIle: 2.307 ± 0.017	2.064PheLys: 2.064 ± 0.015	3.955PheLeu: 3.955 ± 0.024	1.011PheMet: 1.011 ± 0.01	1.905PheAsn: 1.905 ± 0.016	1.858PhePro: 1.858 ± 0.016	1.64PheGln: 1.64 ± 0.013	2.257PheArg: 2.257 ± 0.017	3.469PheSer: 3.469 ± 0.021	2.324PheThr: 2.324 ± 0.017	2.966PheVal: 2.966 ± 0.018	0.527PheTrp: 0.527 ± 0.007	1.517PheTyr: 1.517 ± 0.018	0.017PheXaa: 0.017 ± 0.001
Gly	3.614GlyAla: 3.614 ± 0.032	1.155GlyCys: 1.155 ± 0.015	2.756GlyAsp: 2.756 ± 0.018	3.245GlyGlu: 3.245 ± 0.026	2.388GlyPhe: 2.388 ± 0.02	4.265GlyGly: 4.265 ± 0.055	1.34GlyHis: 1.34 ± 0.015	2.928GlyIle: 2.928 ± 0.019	3.521GlyLys: 3.521 ± 0.028	3.908GlyLeu: 3.908 ± 0.027	1.331GlyMet: 1.331 ± 0.013	2.556GlyAsn: 2.556 ± 0.025	2.425GlyPro: 2.425 ± 0.035	2.168GlyGln: 2.168 ± 0.026	3.325GlyArg: 3.325 ± 0.023	4.391GlySer: 4.391 ± 0.031	2.98GlyThr: 2.98 ± 0.023	3.418GlyVal: 3.418 ± 0.021	0.657GlyTrp: 0.657 ± 0.008	1.895GlyTyr: 1.895 ± 0.022	0.013GlyXaa: 0.013 ± 0.001
His	1.424HisAla: 1.424 ± 0.012	0.498HisCys: 0.498 ± 0.007	1.067HisAsp: 1.067 ± 0.011	1.327HisGlu: 1.327 ± 0.014	1.235HisPhe: 1.235 ± 0.013	1.423HisGly: 1.423 ± 0.016	0.885HisHis: 0.885 ± 0.014	1.285HisIle: 1.285 ± 0.014	1.105HisLys: 1.105 ± 0.013	2.178HisLeu: 2.178 ± 0.016	0.574HisMet: 0.574 ± 0.007	0.968HisAsn: 0.968 ± 0.01	1.245HisPro: 1.245 ± 0.013	1.11HisGln: 1.11 ± 0.011	1.575HisArg: 1.575 ± 0.013	1.737HisSer: 1.737 ± 0.015	1.114HisThr: 1.114 ± 0.012	1.636HisVal: 1.636 ± 0.015	0.294HisTrp: 0.294 ± 0.006	0.753HisTyr: 0.753 ± 0.008	0.006HisXaa: 0.006 ± 0.001
Ile	3.875IleAla: 3.875 ± 0.021	1.143IleCys: 1.143 ± 0.013	3.301IleAsp: 3.301 ± 0.018	3.7IleGlu: 3.7 ± 0.021	2.583IlePhe: 2.583 ± 0.022	3.205IleGly: 3.205 ± 0.02	1.339IleHis: 1.339 ± 0.012	3.011IleIle: 3.011 ± 0.02	2.841IleLys: 2.841 ± 0.018	4.907IleLeu: 4.907 ± 0.024	1.204IleMet: 1.204 ± 0.012	2.421IleAsn: 2.421 ± 0.016	2.882IlePro: 2.882 ± 0.021	2.136IleGln: 2.136 ± 0.016	3.426IleArg: 3.426 ± 0.02	4.478IleSer: 4.478 ± 0.023	3.033IleThr: 3.033 ± 0.019	3.821IleVal: 3.821 ± 0.023	0.706IleTrp: 0.706 ± 0.015	1.604IleTyr: 1.604 ± 0.016	0.014IleXaa: 0.014 ± 0.001
Lys	3.804LysAla: 3.804 ± 0.029	1.312LysCys: 1.312 ± 0.016	3.068LysAsp: 3.068 ± 0.023	4.666LysGlu: 4.666 ± 0.033	2.386LysPhe: 2.386 ± 0.016	2.557LysGly: 2.557 ± 0.019	1.3LysHis: 1.3 ± 0.011	3.672LysIle: 3.672 ± 0.02	5.868LysLys: 5.868 ± 0.044	5.333LysLeu: 5.333 ± 0.027	1.9LysMet: 1.9 ± 0.015	3.301LysAsn: 3.301 ± 0.018	2.7LysPro: 2.7 ± 0.031	2.314LysGln: 2.314 ± 0.018	4.096LysArg: 4.096 ± 0.023	4.694LysSer: 4.694 ± 0.028	3.922LysThr: 3.922 ± 0.023	3.578LysVal: 3.578 ± 0.023	0.817LysTrp: 0.817 ± 0.01	1.918LysTyr: 1.918 ± 0.015	0.023LysXaa: 0.023 ± 0.002
Leu	6.117LeuAla: 6.117 ± 0.032	1.558LeuCys: 1.558 ± 0.014	4.407LeuAsp: 4.407 ± 0.026	5.806LeuGlu: 5.806 ± 0.033	4.035LeuPhe: 4.035 ± 0.029	4.011LeuGly: 4.011 ± 0.024	2.048LeuHis: 2.048 ± 0.018	4.674LeuIle: 4.674 ± 0.026	5.518LeuLys: 5.518 ± 0.029	8.191LeuLeu: 8.191 ± 0.04	2.131LeuMet: 2.131 ± 0.016	4.037LeuAsn: 4.037 ± 0.023	4.491LeuPro: 4.491 ± 0.036	3.561LeuGln: 3.561 ± 0.021	4.987LeuArg: 4.987 ± 0.024	6.572LeuSer: 6.572 ± 0.029	4.736LeuThr: 4.736 ± 0.025	5.181LeuVal: 5.181 ± 0.025	0.899LeuTrp: 0.899 ± 0.01	2.182LeuTyr: 2.182 ± 0.017	0.027LeuXaa: 0.027 ± 0.002
Met	1.81MetAla: 1.81 ± 0.013	0.481MetCys: 0.481 ± 0.007	1.412MetAsp: 1.412 ± 0.013	1.736MetGlu: 1.736 ± 0.014	1.071MetPhe: 1.071 ± 0.011	1.27MetGly: 1.27 ± 0.014	0.552MetHis: 0.552 ± 0.007	1.385MetIle: 1.385 ± 0.012	1.692MetLys: 1.692 ± 0.015	2.143MetLeu: 2.143 ± 0.013	0.842MetMet: 0.842 ± 0.011	1.214MetAsn: 1.214 ± 0.011	1.208MetPro: 1.208 ± 0.013	0.994MetGln: 0.994 ± 0.011	1.561MetArg: 1.561 ± 0.014	2.113MetSer: 2.113 ± 0.015	1.53MetThr: 1.53 ± 0.012	1.462MetVal: 1.462 ± 0.01	0.294MetTrp: 0.294 ± 0.005	0.656MetTyr: 0.656 ± 0.008	0.005MetXaa: 0.005 ± 0.001
Asn	3.053AsnAla: 3.053 ± 0.017	0.949AsnCys: 0.949 ± 0.011	2.499AsnAsp: 2.499 ± 0.017	3.126AsnGlu: 3.126 ± 0.02	2.049AsnPhe: 2.049 ± 0.018	3.257AsnGly: 3.257 ± 0.023	1.097AsnHis: 1.097 ± 0.009	2.422AsnIle: 2.422 ± 0.017	2.36AsnLys: 2.36 ± 0.015	3.898AsnLeu: 3.898 ± 0.021	1.145AsnMet: 1.145 ± 0.01	2.302AsnAsn: 2.302 ± 0.02	2.257AsnPro: 2.257 ± 0.018	1.897AsnGln: 1.897 ± 0.016	2.687AsnArg: 2.687 ± 0.019	3.795AsnSer: 3.795 ± 0.025	2.496AsnThr: 2.496 ± 0.016	3.137AsnVal: 3.137 ± 0.018	0.567AsnTrp: 0.567 ± 0.007	1.466AsnTyr: 1.466 ± 0.016	0.017AsnXaa: 0.017 ± 0.001
Pro	3.6ProAla: 3.6 ± 0.038	0.737ProCys: 0.737 ± 0.01	2.464ProAsp: 2.464 ± 0.029	3.111ProGlu: 3.111 ± 0.026	1.973ProPhe: 1.973 ± 0.016	2.782ProGly: 2.782 ± 0.051	1.101ProHis: 1.101 ± 0.011	2.841ProIle: 2.841 ± 0.022	2.96ProLys: 2.96 ± 0.019	3.906ProLeu: 3.906 ± 0.034	1.148ProMet: 1.148 ± 0.011	2.226ProAsn: 2.226 ± 0.016	4.105ProPro: 4.105 ± 0.045	2.001ProGln: 2.001 ± 0.021	2.678ProArg: 2.678 ± 0.018	4.393ProSer: 4.393 ± 0.029	3.544ProThr: 3.544 ± 0.044	3.311ProVal: 3.311 ± 0.028	0.459ProTrp: 0.459 ± 0.007	1.297ProTyr: 1.297 ± 0.011	0.01ProXaa: 0.01 ± 0.001
Gln	2.412GlnAla: 2.412 ± 0.023	0.914GlnCys: 0.914 ± 0.019	1.617GlnAsp: 1.617 ± 0.012	2.427GlnGlu: 2.427 ± 0.019	1.871GlnPhe: 1.871 ± 0.02	1.66GlnGly: 1.66 ± 0.015	1.11GlnHis: 1.11 ± 0.012	2.348GlnIle: 2.348 ± 0.016	2.948GlnLys: 2.948 ± 0.021	3.947GlnLeu: 3.947 ± 0.025	1.318GlnMet: 1.318 ± 0.014	2.114GlnAsn: 2.114 ± 0.017	2.057GlnPro: 2.057 ± 0.019	2.926GlnGln: 2.926 ± 0.053	2.399GlnArg: 2.399 ± 0.019	2.91GlnSer: 2.91 ± 0.022	2.143GlnThr: 2.143 ± 0.018	2.344GlnVal: 2.344 ± 0.017	0.475GlnTrp: 0.475 ± 0.008	1.25GlnTyr: 1.25 ± 0.014	0.013GlnXaa: 0.013 ± 0.001
Arg	3.737ArgAla: 3.737 ± 0.024	1.038ArgCys: 1.038 ± 0.012	2.769ArgAsp: 2.769 ± 0.023	3.703ArgGlu: 3.703 ± 0.024	2.331ArgPhe: 2.331 ± 0.02	2.953ArgGly: 2.953 ± 0.026	1.482ArgHis: 1.482 ± 0.014	3.395ArgIle: 3.395 ± 0.019	4.304ArgLys: 4.304 ± 0.026	5.006ArgLeu: 5.006 ± 0.028	1.559ArgMet: 1.559 ± 0.012	2.985ArgAsn: 2.985 ± 0.018	2.784ArgPro: 2.784 ± 0.022	2.527ArgGln: 2.527 ± 0.018	5.226ArgArg: 5.226 ± 0.037	4.446ArgSer: 4.446 ± 0.028	3.164ArgThr: 3.164 ± 0.023	3.367ArgVal: 3.367 ± 0.019	0.709ArgTrp: 0.709 ± 0.009	1.551ArgTyr: 1.551 ± 0.012	0.017ArgXaa: 0.017 ± 0.001
Ser	5.364SerAla: 5.364 ± 0.027	1.446SerCys: 1.446 ± 0.017	4.191SerAsp: 4.191 ± 0.024	4.914SerGlu: 4.914 ± 0.028	3.157SerPhe: 3.157 ± 0.02	4.469SerGly: 4.469 ± 0.033	1.664SerHis: 1.664 ± 0.014	4.403SerIle: 4.403 ± 0.022	4.401SerLys: 4.401 ± 0.022	6.546SerLeu: 6.546 ± 0.034	1.861SerMet: 1.861 ± 0.014	3.521SerAsn: 3.521 ± 0.023	4.28SerPro: 4.28 ± 0.031	3.062SerGln: 3.062 ± 0.02	4.466SerArg: 4.466 ± 0.025	8.531SerSer: 8.531 ± 0.061	5.63SerThr: 5.63 ± 0.043	4.881SerVal: 4.881 ± 0.026	0.827SerTrp: 0.827 ± 0.009	1.967SerTyr: 1.967 ± 0.019	0.019SerXaa: 0.019 ± 0.001
Thr	4.162ThrAla: 4.162 ± 0.029	1.259ThrCys: 1.259 ± 0.017	2.927ThrAsp: 2.927 ± 0.045	3.519ThrGlu: 3.519 ± 0.028	2.458ThrPhe: 2.458 ± 0.018	3.01ThrGly: 3.01 ± 0.026	1.219ThrHis: 1.219 ± 0.012	3.605ThrIle: 3.605 ± 0.023	3.327ThrLys: 3.327 ± 0.024	4.743ThrLeu: 4.743 ± 0.024	1.425ThrMet: 1.425 ± 0.013	2.504ThrAsn: 2.504 ± 0.017	3.402ThrPro: 3.402 ± 0.029	2.008ThrGln: 2.008 ± 0.016	3.008ThrArg: 3.008 ± 0.02	5.331ThrSer: 5.331 ± 0.038	4.889ThrThr: 4.889 ± 0.065	4.247ThrVal: 4.247 ± 0.028	0.681ThrTrp: 0.681 ± 0.009	1.511ThrTyr: 1.511 ± 0.014	0.012ThrXaa: 0.012 ± 0.001
Val	4.548ValAla: 4.548 ± 0.025	1.328ValCys: 1.328 ± 0.015	3.565ValAsp: 3.565 ± 0.02	4.389ValGlu: 4.389 ± 0.03	3.018ValPhe: 3.018 ± 0.02	3.146ValGly: 3.146 ± 0.02	1.466ValHis: 1.466 ± 0.012	3.575ValIle: 3.575 ± 0.023	3.883ValLys: 3.883 ± 0.025	5.642ValLeu: 5.642 ± 0.031	1.545ValMet: 1.545 ± 0.014	2.871ValAsn: 2.871 ± 0.018	3.134ValPro: 3.134 ± 0.021	2.553ValGln: 2.553 ± 0.016	3.431ValArg: 3.431 ± 0.022	4.661ValSer: 4.661 ± 0.024	3.689ValThr: 3.689 ± 0.025	4.423ValVal: 4.423 ± 0.029	0.76ValTrp: 0.76 ± 0.009	1.919ValTyr: 1.919 ± 0.016	0.016ValXaa: 0.016 ± 0.001
Trp	0.824TrpAla: 0.824 ± 0.011	0.221TrpCys: 0.221 ± 0.004	0.571TrpAsp: 0.571 ± 0.008	0.655TrpGlu: 0.655 ± 0.008	0.512TrpPhe: 0.512 ± 0.008	0.504TrpGly: 0.504 ± 0.009	0.27TrpHis: 0.27 ± 0.005	0.738TrpIle: 0.738 ± 0.009	0.948TrpLys: 0.948 ± 0.009	1.009TrpLeu: 1.009 ± 0.013	0.364TrpMet: 0.364 ± 0.005	0.622TrpAsn: 0.622 ± 0.007	0.502TrpPro: 0.502 ± 0.007	0.486TrpGln: 0.486 ± 0.007	0.747TrpArg: 0.747 ± 0.01	0.899TrpSer: 0.899 ± 0.012	0.791TrpThr: 0.791 ± 0.009	0.575TrpVal: 0.575 ± 0.008	0.157TrpTrp: 0.157 ± 0.004	0.33TrpTyr: 0.33 ± 0.006	0.003TrpXaa: 0.003 ± 0.0
Tyr	1.754TyrAla: 1.754 ± 0.015	0.665TyrCys: 0.665 ± 0.011	1.622TyrAsp: 1.622 ± 0.014	1.789TyrGlu: 1.789 ± 0.015	1.351TyrPhe: 1.351 ± 0.012	1.87TyrGly: 1.87 ± 0.016	0.768TyrHis: 0.768 ± 0.008	1.411TyrIle: 1.411 ± 0.013	1.499TyrLys: 1.499 ± 0.013	2.631TyrLeu: 2.631 ± 0.022	0.769TyrMet: 0.769 ± 0.01	1.305TyrAsn: 1.305 ± 0.012	1.354TyrPro: 1.354 ± 0.021	1.275TyrGln: 1.275 ± 0.011	1.66TyrArg: 1.66 ± 0.013	2.188TyrSer: 2.188 ± 0.022	1.558TyrThr: 1.558 ± 0.014	1.78TyrVal: 1.78 ± 0.014	0.379TyrTrp: 0.379 ± 0.006	1.063TyrTyr: 1.063 ± 0.013	0.012TyrXaa: 0.012 ± 0.001
Xaa	0.017XaaAla: 0.017 ± 0.001	0.007XaaCys: 0.007 ± 0.001	0.013XaaAsp: 0.013 ± 0.001	0.014XaaGlu: 0.014 ± 0.001	0.014XaaPhe: 0.014 ± 0.001	0.01XaaGly: 0.01 ± 0.001	0.007XaaHis: 0.007 ± 0.001	0.014XaaIle: 0.014 ± 0.001	0.018XaaLys: 0.018 ± 0.001	0.026XaaLeu: 0.026 ± 0.002	0.005XaaMet: 0.005 ± 0.001	0.014XaaAsn: 0.014 ± 0.001	0.015XaaPro: 0.015 ± 0.001	0.012XaaGln: 0.012 ± 0.001	0.017XaaArg: 0.017 ± 0.001	0.024XaaSer: 0.024 ± 0.001	0.017XaaThr: 0.017 ± 0.001	0.015XaaVal: 0.015 ± 0.001	0.003XaaTrp: 0.003 ± 0.001	0.009XaaTyr: 0.009 ± 0.001	0.337XaaXaa: 0.337 ± 0.027

Statistics based on 35026 proteins (10578374 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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