Burkholderia phage BcepMu (isolate -/United States/Summer/2002) (Bacteriophage BcepMu)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Bcepmuvirus; Burkholderia virus BcepMu

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6QIA4|Q6QIA4_BPBMU Gp45 OS=Burkholderia phage BcepMu (isolate -/United States/Summer/2002) OX=1283335 GN=BcepMu45 PE=4 SV=1
MM1 pKa = 7.51FLTHH5 pKa = 6.41ITTEE9 pKa = 4.19GEE11 pKa = 3.76RR12 pKa = 11.84WDD14 pKa = 3.92QIAYY18 pKa = 9.87RR19 pKa = 11.84YY20 pKa = 10.35YY21 pKa = 10.76GDD23 pKa = 3.47PFAYY27 pKa = 9.81EE28 pKa = 4.49RR29 pKa = 11.84IIAANPSVPITAVLASGIALSIPVVAADD57 pKa = 3.98DD58 pKa = 4.06VADD61 pKa = 4.13EE62 pKa = 4.74EE63 pKa = 4.71LPPWMRR69 pKa = 3.95

Molecular weight: 7.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6QIA6|Q6QIA6_BPBMU Gp43 (Fragment) OS=Burkholderia phage BcepMu (isolate -/United States/Summer/2002) OX=1283335 GN=BcepMu43 PE=4 SV=1
MM1 pKa = 7.57ASEE4 pKa = 4.93FYY6 pKa = 10.53IGVKK10 pKa = 10.01IGATLLGSFGAALSGTRR27 pKa = 11.84TTLNGLGRR35 pKa = 11.84VADD38 pKa = 4.21EE39 pKa = 3.62LRR41 pKa = 11.84ARR43 pKa = 11.84HH44 pKa = 5.45TRR46 pKa = 11.84LGDD49 pKa = 3.19AMARR53 pKa = 11.84AVAHH57 pKa = 6.07PMRR60 pKa = 11.84NIAEE64 pKa = 4.1LRR66 pKa = 11.84GQYY69 pKa = 10.5DD70 pKa = 3.37RR71 pKa = 11.84LGRR74 pKa = 11.84TIDD77 pKa = 3.44QVQAKK82 pKa = 8.79QAALATRR89 pKa = 11.84LARR92 pKa = 11.84GAALRR97 pKa = 11.84EE98 pKa = 3.92QRR100 pKa = 11.84QGLGADD106 pKa = 3.42MLGTYY111 pKa = 8.0ATAAATAAPVIGAVRR126 pKa = 11.84QAATFEE132 pKa = 4.2AGLRR136 pKa = 11.84DD137 pKa = 3.5IAITGNLTRR146 pKa = 11.84DD147 pKa = 3.09EE148 pKa = 4.22EE149 pKa = 4.22FRR151 pKa = 11.84IGEE154 pKa = 4.27TMRR157 pKa = 11.84RR158 pKa = 11.84AALATSQGHH167 pKa = 5.08NSILEE172 pKa = 4.22GVGTLVAAGMDD183 pKa = 3.48AKK185 pKa = 10.87EE186 pKa = 4.62AGQKK190 pKa = 10.64SNLLGRR196 pKa = 11.84VATATNADD204 pKa = 3.77MKK206 pKa = 10.84DD207 pKa = 3.54LAGMVYY213 pKa = 10.26SFSEE217 pKa = 4.06TLGIKK222 pKa = 10.17GDD224 pKa = 3.84AALKK228 pKa = 9.69EE229 pKa = 4.3AFNRR233 pKa = 11.84AAYY236 pKa = 9.71GGKK239 pKa = 10.07LGRR242 pKa = 11.84FEE244 pKa = 5.05LKK246 pKa = 10.8DD247 pKa = 3.31MAKK250 pKa = 10.47ALPEE254 pKa = 3.77MTAAFAAKK262 pKa = 10.08GIKK265 pKa = 10.08GQDD268 pKa = 3.2ALTQIIASLEE278 pKa = 4.07VGRR281 pKa = 11.84EE282 pKa = 3.94GAGSGDD288 pKa = 3.37EE289 pKa = 4.43AVTNLRR295 pKa = 11.84NWLSHH300 pKa = 5.73MNAKK304 pKa = 8.64ATIDD308 pKa = 3.52AYY310 pKa = 10.11KK311 pKa = 10.38KK312 pKa = 10.77AGVDD316 pKa = 3.73YY317 pKa = 10.29QKK319 pKa = 11.73SMSNLVAGGYY329 pKa = 9.46SSYY332 pKa = 11.01EE333 pKa = 3.64GSLQIAQKK341 pKa = 10.33FIASRR346 pKa = 11.84GDD348 pKa = 3.14AFMKK352 pKa = 9.23QWKK355 pKa = 9.13AAGAKK360 pKa = 9.93GDD362 pKa = 3.88EE363 pKa = 4.14EE364 pKa = 4.25AQRR367 pKa = 11.84KK368 pKa = 8.89LMEE371 pKa = 4.45SFGLNEE377 pKa = 4.17VFQDD381 pKa = 3.12IQTINHH387 pKa = 7.1LLAMRR392 pKa = 11.84QGWDD396 pKa = 3.12KK397 pKa = 11.1YY398 pKa = 7.82QQNKK402 pKa = 9.53KK403 pKa = 11.01DD404 pKa = 3.64MGSAQALNTIDD415 pKa = 3.16QDD417 pKa = 3.94YY418 pKa = 10.72VRR420 pKa = 11.84RR421 pKa = 11.84AEE423 pKa = 4.12LATVAWGRR431 pKa = 11.84FQTQIADD438 pKa = 3.58LGITVGRR445 pKa = 11.84ALLPSLTDD453 pKa = 3.29LMNTVTPLIQRR464 pKa = 11.84TAQFAAAHH472 pKa = 6.54PGLIRR477 pKa = 11.84GVVGFATAVIGMKK490 pKa = 10.1VATLAAGWGLNFFVKK505 pKa = 10.55SPLNMVSTALTTVGAKK521 pKa = 6.89WTLFRR526 pKa = 11.84ALWAGGGSRR535 pKa = 11.84LSTVFQIFGMGAGAAGKK552 pKa = 8.95FAAVIGRR559 pKa = 11.84AGSLFMGFGRR569 pKa = 11.84GALVVGRR576 pKa = 11.84ALLPFGQGMLMTFIGPLRR594 pKa = 11.84LLAQGGMLLARR605 pKa = 11.84VLGGQLVNGLMLAGRR620 pKa = 11.84AVLWLGRR627 pKa = 11.84ALMLNPIGIAITAIAVGAYY646 pKa = 10.36LIYY649 pKa = 10.4RR650 pKa = 11.84YY651 pKa = 6.93WTPIKK656 pKa = 10.55QFFGGIWASIRR667 pKa = 11.84TAFAGVLGWFGVGLPKK683 pKa = 10.46TFTDD687 pKa = 4.81FGSHH691 pKa = 7.57LIDD694 pKa = 3.79GLVNGIRR701 pKa = 11.84NRR703 pKa = 11.84FTAAKK708 pKa = 7.81NTLIEE713 pKa = 4.47FGSNVKK719 pKa = 9.94AWFANTLGIKK729 pKa = 9.99SPSRR733 pKa = 11.84VFMGFGDD740 pKa = 4.89NIAQGAAIGIGRR752 pKa = 11.84SSAVAARR759 pKa = 11.84AAAGMATQAAAAASLQRR776 pKa = 11.84INAARR781 pKa = 11.84GGSPAGASVAGSGITVHH798 pKa = 6.72FSPTITVQGGSPDD811 pKa = 3.55GVKK814 pKa = 10.7DD815 pKa = 3.82QVKK818 pKa = 10.38QGLNLSLRR826 pKa = 11.84DD827 pKa = 4.07LEE829 pKa = 5.47RR830 pKa = 11.84MLDD833 pKa = 3.55DD834 pKa = 5.13LLAKK838 pKa = 10.08RR839 pKa = 11.84EE840 pKa = 3.81RR841 pKa = 11.84RR842 pKa = 11.84AYY844 pKa = 10.42RR845 pKa = 11.84SS846 pKa = 3.23

Molecular weight: 89.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11923

67

846

225.0

24.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.715 ± 0.468

0.646 ± 0.087

6.014 ± 0.326

5.661 ± 0.374

3.229 ± 0.22

8.11 ± 0.461

1.61 ± 0.177

4.697 ± 0.22

4.361 ± 0.307

9.134 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.29 ± 0.165

3.095 ± 0.251

4.378 ± 0.26

4.202 ± 0.182

7.087 ± 0.39

5.603 ± 0.309

5.871 ± 0.399

7.314 ± 0.299

1.283 ± 0.131

2.701 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski