Amino acid dipepetide frequency for Firmicutes bacterium CAG:94

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.94AlaAla: 10.94 ± 0.177	1.644AlaCys: 1.644 ± 0.052	4.131AlaAsp: 4.131 ± 0.09	5.288AlaGlu: 5.288 ± 0.096	3.57AlaPhe: 3.57 ± 0.081	8.017AlaGly: 8.017 ± 0.137	1.712AlaHis: 1.712 ± 0.061	4.647AlaIle: 4.647 ± 0.098	4.037AlaLys: 4.037 ± 0.087	10.794AlaLeu: 10.794 ± 0.179	2.589AlaMet: 2.589 ± 0.064	2.153AlaAsn: 2.153 ± 0.048	3.752AlaPro: 3.752 ± 0.095	5.082AlaGln: 5.082 ± 0.097	4.663AlaArg: 4.663 ± 0.096	4.575AlaSer: 4.575 ± 0.092	3.934AlaThr: 3.934 ± 0.086	7.789AlaVal: 7.789 ± 0.14	0.978AlaTrp: 0.978 ± 0.045	2.889AlaTyr: 2.889 ± 0.065	0.001AlaXaa: 0.001 ± 0.001
Cys	1.444CysAla: 1.444 ± 0.052	0.4CysCys: 0.4 ± 0.027	0.756CysAsp: 0.756 ± 0.032	0.764CysGlu: 0.764 ± 0.04	0.619CysPhe: 0.619 ± 0.029	1.84CysGly: 1.84 ± 0.055	0.376CysHis: 0.376 ± 0.028	0.699CysIle: 0.699 ± 0.03	0.744CysLys: 0.744 ± 0.038	1.431CysLeu: 1.431 ± 0.046	0.333CysMet: 0.333 ± 0.022	0.453CysAsn: 0.453 ± 0.028	0.922CysPro: 0.922 ± 0.041	0.811CysGln: 0.811 ± 0.037	1.0CysArg: 1.0 ± 0.044	0.882CysSer: 0.882 ± 0.041	0.801CysThr: 0.801 ± 0.037	1.2CysVal: 1.2 ± 0.044	0.203CysTrp: 0.203 ± 0.019	0.522CysTyr: 0.522 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	4.282AspAla: 4.282 ± 0.078	0.787AspCys: 0.787 ± 0.035	2.078AspAsp: 2.078 ± 0.065	3.289AspGlu: 3.289 ± 0.077	2.584AspPhe: 2.584 ± 0.064	4.585AspGly: 4.585 ± 0.1	0.873AspHis: 0.873 ± 0.034	3.166AspIle: 3.166 ± 0.077	2.451AspLys: 2.451 ± 0.071	4.739AspLeu: 4.739 ± 0.089	1.513AspMet: 1.513 ± 0.05	1.553AspAsn: 1.553 ± 0.053	2.396AspPro: 2.396 ± 0.067	1.581AspGln: 1.581 ± 0.046	2.321AspArg: 2.321 ± 0.066	2.855AspSer: 2.855 ± 0.071	3.044AspThr: 3.044 ± 0.066	3.684AspVal: 3.684 ± 0.071	0.665AspTrp: 0.665 ± 0.036	2.071AspTyr: 2.071 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	6.811GluAla: 6.811 ± 0.109	0.736GluCys: 0.736 ± 0.035	4.162GluAsp: 4.162 ± 0.089	7.015GluGlu: 7.015 ± 0.127	2.183GluPhe: 2.183 ± 0.069	5.426GluGly: 5.426 ± 0.1	1.342GluHis: 1.342 ± 0.047	3.701GluIle: 3.701 ± 0.088	4.777GluLys: 4.777 ± 0.107	6.78GluLeu: 6.78 ± 0.102	2.01GluMet: 2.01 ± 0.054	3.166GluAsn: 3.166 ± 0.065	2.624GluPro: 2.624 ± 0.07	2.901GluGln: 2.901 ± 0.07	4.067GluArg: 4.067 ± 0.082	3.434GluSer: 3.434 ± 0.07	3.447GluThr: 3.447 ± 0.068	4.15GluVal: 4.15 ± 0.105	0.577GluTrp: 0.577 ± 0.032	2.31GluTyr: 2.31 ± 0.063	0.0GluXaa: 0.0 ± 0.0
Phe	3.506PheAla: 3.506 ± 0.08	0.799PheCys: 0.799 ± 0.033	2.238PheAsp: 2.238 ± 0.062	2.14PheGlu: 2.14 ± 0.063	1.902PhePhe: 1.902 ± 0.061	3.312PheGly: 3.312 ± 0.081	0.875PheHis: 0.875 ± 0.032	1.829PheIle: 1.829 ± 0.069	1.128PheLys: 1.128 ± 0.041	4.376PheLeu: 4.376 ± 0.089	0.746PheMet: 0.746 ± 0.034	1.072PheAsn: 1.072 ± 0.041	1.807PhePro: 1.807 ± 0.052	1.849PheGln: 1.849 ± 0.053	1.958PheArg: 1.958 ± 0.056	2.936PheSer: 2.936 ± 0.083	2.569PheThr: 2.569 ± 0.062	2.667PheVal: 2.667 ± 0.062	0.512PheTrp: 0.512 ± 0.031	1.499PheTyr: 1.499 ± 0.055	0.0PheXaa: 0.0 ± 0.0
Gly	6.987GlyAla: 6.987 ± 0.13	1.467GlyCys: 1.467 ± 0.055	4.455GlyAsp: 4.455 ± 0.085	6.434GlyGlu: 6.434 ± 0.114	3.37GlyPhe: 3.37 ± 0.077	6.784GlyGly: 6.784 ± 0.158	1.619GlyHis: 1.619 ± 0.066	4.477GlyIle: 4.477 ± 0.08	5.192GlyLys: 5.192 ± 0.088	7.893GlyLeu: 7.893 ± 0.131	2.42GlyMet: 2.42 ± 0.061	2.559GlyAsn: 2.559 ± 0.056	2.478GlyPro: 2.478 ± 0.062	3.358GlyGln: 3.358 ± 0.09	4.165GlyArg: 4.165 ± 0.088	4.329GlySer: 4.329 ± 0.085	4.28GlyThr: 4.28 ± 0.088	6.542GlyVal: 6.542 ± 0.111	0.938GlyTrp: 0.938 ± 0.042	2.865GlyTyr: 2.865 ± 0.063	0.006GlyXaa: 0.006 ± 0.003
His	1.391HisAla: 1.391 ± 0.054	0.413HisCys: 0.413 ± 0.028	0.82HisAsp: 0.82 ± 0.039	0.753HisGlu: 0.753 ± 0.038	0.835HisPhe: 0.835 ± 0.035	1.751HisGly: 1.751 ± 0.065	0.542HisHis: 0.542 ± 0.033	1.131HisIle: 1.131 ± 0.041	0.764HisLys: 0.764 ± 0.032	1.967HisLeu: 1.967 ± 0.063	0.437HisMet: 0.437 ± 0.024	0.733HisAsn: 0.733 ± 0.032	1.202HisPro: 1.202 ± 0.053	0.875HisGln: 0.875 ± 0.042	1.27HisArg: 1.27 ± 0.043	1.067HisSer: 1.067 ± 0.048	1.146HisThr: 1.146 ± 0.044	1.277HisVal: 1.277 ± 0.043	0.255HisTrp: 0.255 ± 0.019	0.753HisTyr: 0.753 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	4.705IleAla: 4.705 ± 0.089	0.826IleCys: 0.826 ± 0.038	2.861IleAsp: 2.861 ± 0.089	2.67IleGlu: 2.67 ± 0.071	1.832IlePhe: 1.832 ± 0.057	3.733IleGly: 3.733 ± 0.084	1.103IleHis: 1.103 ± 0.041	2.485IleIle: 2.485 ± 0.066	1.945IleLys: 1.945 ± 0.062	5.245IleLeu: 5.245 ± 0.094	1.009IleMet: 1.009 ± 0.042	1.557IleAsn: 1.557 ± 0.055	2.853IlePro: 2.853 ± 0.072	2.233IleGln: 2.233 ± 0.063	2.728IleArg: 2.728 ± 0.076	3.228IleSer: 3.228 ± 0.077	3.223IleThr: 3.223 ± 0.08	3.5IleVal: 3.5 ± 0.081	0.451IleTrp: 0.451 ± 0.028	1.56IleTyr: 1.56 ± 0.05	0.0IleXaa: 0.0 ± 0.0
Lys	4.841LysAla: 4.841 ± 0.086	0.53LysCys: 0.53 ± 0.034	2.583LysAsp: 2.583 ± 0.074	4.185LysGlu: 4.185 ± 0.097	1.295LysPhe: 1.295 ± 0.046	3.536LysGly: 3.536 ± 0.078	0.734LysHis: 0.734 ± 0.034	2.557LysIle: 2.557 ± 0.07	3.323LysLys: 3.323 ± 0.095	4.649LysLeu: 4.649 ± 0.082	1.322LysMet: 1.322 ± 0.045	1.826LysAsn: 1.826 ± 0.056	1.962LysPro: 1.962 ± 0.053	1.606LysGln: 1.606 ± 0.058	2.819LysArg: 2.819 ± 0.072	2.273LysSer: 2.273 ± 0.064	2.563LysThr: 2.563 ± 0.063	3.231LysVal: 3.231 ± 0.068	0.423LysTrp: 0.423 ± 0.024	1.683LysTyr: 1.683 ± 0.062	0.003LysXaa: 0.003 ± 0.002
Leu	9.552LeuAla: 9.552 ± 0.152	2.168LeuCys: 2.168 ± 0.064	5.218LeuAsp: 5.218 ± 0.082	8.107LeuGlu: 8.107 ± 0.126	4.345LeuPhe: 4.345 ± 0.106	8.272LeuGly: 8.272 ± 0.134	2.145LeuHis: 2.145 ± 0.065	4.177LeuIle: 4.177 ± 0.101	4.107LeuLys: 4.107 ± 0.07	11.562LeuLeu: 11.562 ± 0.218	2.312LeuMet: 2.312 ± 0.057	2.793LeuAsn: 2.793 ± 0.067	5.568LeuPro: 5.568 ± 0.118	3.598LeuGln: 3.598 ± 0.079	6.052LeuArg: 6.052 ± 0.112	6.844LeuSer: 6.844 ± 0.12	5.227LeuThr: 5.227 ± 0.088	7.061LeuVal: 7.061 ± 0.12	1.171LeuTrp: 1.171 ± 0.043	3.479LeuTyr: 3.479 ± 0.081	0.004LeuXaa: 0.004 ± 0.002
Met	2.836MetAla: 2.836 ± 0.067	0.286MetCys: 0.286 ± 0.021	1.487MetAsp: 1.487 ± 0.045	2.364MetGlu: 2.364 ± 0.067	0.768MetPhe: 0.768 ± 0.031	2.162MetGly: 2.162 ± 0.064	0.357MetHis: 0.357 ± 0.024	1.101MetIle: 1.101 ± 0.043	1.733MetLys: 1.733 ± 0.046	2.386MetLeu: 2.386 ± 0.06	0.651MetMet: 0.651 ± 0.038	0.995MetAsn: 0.995 ± 0.035	1.129MetPro: 1.129 ± 0.04	0.753MetGln: 0.753 ± 0.032	1.265MetArg: 1.265 ± 0.048	1.325MetSer: 1.325 ± 0.048	1.362MetThr: 1.362 ± 0.049	1.894MetVal: 1.894 ± 0.056	0.188MetTrp: 0.188 ± 0.017	0.691MetTyr: 0.691 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	2.75AsnAla: 2.75 ± 0.059	0.474AsnCys: 0.474 ± 0.031	1.335AsnAsp: 1.335 ± 0.045	1.57AsnGlu: 1.57 ± 0.054	1.265AsnPhe: 1.265 ± 0.045	2.822AsnGly: 2.822 ± 0.069	0.586AsnHis: 0.586 ± 0.03	1.977AsnIle: 1.977 ± 0.065	1.261AsnLys: 1.261 ± 0.046	3.287AsnLeu: 3.287 ± 0.069	0.826AsnMet: 0.826 ± 0.038	1.009AsnAsn: 1.009 ± 0.043	1.77AsnPro: 1.77 ± 0.054	1.183AsnGln: 1.183 ± 0.042	1.748AsnArg: 1.748 ± 0.049	1.696AsnSer: 1.696 ± 0.053	1.806AsnThr: 1.806 ± 0.057	2.285AsnVal: 2.285 ± 0.058	0.361AsnTrp: 0.361 ± 0.024	1.131AsnTyr: 1.131 ± 0.042	0.001AsnXaa: 0.001 ± 0.001
Pro	4.473ProAla: 4.473 ± 0.096	0.687ProCys: 0.687 ± 0.029	2.347ProAsp: 2.347 ± 0.062	3.919ProGlu: 3.919 ± 0.077	1.801ProPhe: 1.801 ± 0.052	4.052ProGly: 4.052 ± 0.098	0.916ProHis: 0.916 ± 0.034	1.977ProIle: 1.977 ± 0.057	1.823ProLys: 1.823 ± 0.058	4.335ProLeu: 4.335 ± 0.09	1.15ProMet: 1.15 ± 0.047	1.234ProAsn: 1.234 ± 0.039	1.748ProPro: 1.748 ± 0.071	2.628ProGln: 2.628 ± 0.064	1.995ProArg: 1.995 ± 0.062	2.284ProSer: 2.284 ± 0.062	2.171ProThr: 2.171 ± 0.067	3.678ProVal: 3.678 ± 0.07	0.628ProTrp: 0.628 ± 0.031	1.547ProTyr: 1.547 ± 0.053	0.0ProXaa: 0.0 ± 0.0
Gln	4.542GlnAla: 4.542 ± 0.114	0.536GlnCys: 0.536 ± 0.031	1.859GlnAsp: 1.859 ± 0.056	3.965GlnGlu: 3.965 ± 0.095	1.301GlnPhe: 1.301 ± 0.042	3.676GlnGly: 3.676 ± 0.081	0.619GlnHis: 0.619 ± 0.031	1.819GlnIle: 1.819 ± 0.051	2.205GlnLys: 2.205 ± 0.057	4.222GlnLeu: 4.222 ± 0.087	1.063GlnMet: 1.063 ± 0.042	1.431GlnAsn: 1.431 ± 0.054	1.761GlnPro: 1.761 ± 0.061	1.798GlnGln: 1.798 ± 0.053	2.731GlnArg: 2.731 ± 0.068	1.776GlnSer: 1.776 ± 0.064	1.616GlnThr: 1.616 ± 0.045	3.474GlnVal: 3.474 ± 0.078	0.536GlnTrp: 0.536 ± 0.031	1.4GlnTyr: 1.4 ± 0.05	0.0GlnXaa: 0.0 ± 0.0
Arg	4.4ArgAla: 4.4 ± 0.089	0.864ArgCys: 0.864 ± 0.037	2.674ArgAsp: 2.674 ± 0.071	4.832ArgGlu: 4.832 ± 0.105	2.332ArgPhe: 2.332 ± 0.059	3.835ArgGly: 3.835 ± 0.081	1.122ArgHis: 1.122 ± 0.041	2.543ArgIle: 2.543 ± 0.065	2.917ArgLys: 2.917 ± 0.071	5.639ArgLeu: 5.639 ± 0.107	1.535ArgMet: 1.535 ± 0.051	1.595ArgAsn: 1.595 ± 0.052	2.389ArgPro: 2.389 ± 0.067	2.697ArgGln: 2.697 ± 0.068	3.892ArgArg: 3.892 ± 0.1	2.392ArgSer: 2.392 ± 0.065	2.236ArgThr: 2.236 ± 0.054	3.82ArgVal: 3.82 ± 0.078	0.687ArgTrp: 0.687 ± 0.032	1.96ArgTyr: 1.96 ± 0.055	0.003ArgXaa: 0.003 ± 0.002
Ser	4.65SerAla: 4.65 ± 0.086	0.802SerCys: 0.802 ± 0.038	2.364SerAsp: 2.364 ± 0.054	2.68SerGlu: 2.68 ± 0.077	2.522SerPhe: 2.522 ± 0.061	4.822SerGly: 4.822 ± 0.106	1.137SerHis: 1.137 ± 0.046	2.784SerIle: 2.784 ± 0.065	2.119SerLys: 2.119 ± 0.06	6.178SerLeu: 6.178 ± 0.118	1.604SerMet: 1.604 ± 0.051	1.473SerAsn: 1.473 ± 0.048	2.739SerPro: 2.739 ± 0.075	2.902SerGln: 2.902 ± 0.067	3.001SerArg: 3.001 ± 0.074	3.576SerSer: 3.576 ± 0.122	2.933SerThr: 2.933 ± 0.092	3.913SerVal: 3.913 ± 0.085	0.71SerTrp: 0.71 ± 0.035	2.044SerTyr: 2.044 ± 0.056	0.001SerXaa: 0.001 ± 0.002
Thr	4.989ThrAla: 4.989 ± 0.094	0.724ThrCys: 0.724 ± 0.032	2.473ThrAsp: 2.473 ± 0.064	2.964ThrGlu: 2.964 ± 0.078	2.06ThrPhe: 2.06 ± 0.06	4.848ThrGly: 4.848 ± 0.1	0.962ThrHis: 0.962 ± 0.041	2.762ThrIle: 2.762 ± 0.067	1.933ThrLys: 1.933 ± 0.062	5.43ThrLeu: 5.43 ± 0.101	1.291ThrMet: 1.291 ± 0.048	1.413ThrAsn: 1.413 ± 0.046	2.932ThrPro: 2.932 ± 0.084	2.073ThrGln: 2.073 ± 0.057	2.321ThrArg: 2.321 ± 0.058	2.689ThrSer: 2.689 ± 0.073	2.705ThrThr: 2.705 ± 0.063	4.887ThrVal: 4.887 ± 0.083	0.613ThrTrp: 0.613 ± 0.033	1.829ThrTyr: 1.829 ± 0.061	0.0ThrXaa: 0.0 ± 0.0
Val	6.383ValAla: 6.383 ± 0.12	1.333ValCys: 1.333 ± 0.037	3.944ValAsp: 3.944 ± 0.089	5.609ValGlu: 5.609 ± 0.086	3.135ValPhe: 3.135 ± 0.076	5.414ValGly: 5.414 ± 0.117	1.262ValHis: 1.262 ± 0.044	3.777ValIle: 3.777 ± 0.09	3.518ValLys: 3.518 ± 0.079	8.276ValLeu: 8.276 ± 0.133	1.886ValMet: 1.886 ± 0.06	2.365ValAsn: 2.365 ± 0.062	3.373ValPro: 3.373 ± 0.062	2.242ValGln: 2.242 ± 0.056	3.678ValArg: 3.678 ± 0.086	4.354ValSer: 4.354 ± 0.083	4.258ValThr: 4.258 ± 0.081	5.701ValVal: 5.701 ± 0.119	0.789ValTrp: 0.789 ± 0.037	2.476ValTyr: 2.476 ± 0.062	0.001ValXaa: 0.001 ± 0.001
Trp	1.006TrpAla: 1.006 ± 0.044	0.155TrpCys: 0.155 ± 0.016	0.666TrpAsp: 0.666 ± 0.031	1.003TrpGlu: 1.003 ± 0.042	0.444TrpPhe: 0.444 ± 0.027	0.925TrpGly: 0.925 ± 0.037	0.249TrpHis: 0.249 ± 0.021	0.414TrpIle: 0.414 ± 0.026	0.579TrpLys: 0.579 ± 0.025	1.128TrpLeu: 1.128 ± 0.048	0.345TrpMet: 0.345 ± 0.025	0.444TrpAsn: 0.444 ± 0.027	0.367TrpPro: 0.367 ± 0.027	0.533TrpGln: 0.533 ± 0.029	0.552TrpArg: 0.552 ± 0.03	0.533TrpSer: 0.533 ± 0.029	0.468TrpThr: 0.468 ± 0.027	0.815TrpVal: 0.815 ± 0.032	0.154TrpTrp: 0.154 ± 0.016	0.416TrpTyr: 0.416 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.695TyrAla: 2.695 ± 0.073	0.568TyrCys: 0.568 ± 0.028	2.063TyrAsp: 2.063 ± 0.063	2.066TyrGlu: 2.066 ± 0.057	1.557TyrPhe: 1.557 ± 0.05	2.8TyrGly: 2.8 ± 0.063	0.85TyrHis: 0.85 ± 0.036	1.724TyrIle: 1.724 ± 0.052	1.388TyrLys: 1.388 ± 0.046	3.445TyrLeu: 3.445 ± 0.072	0.693TyrMet: 0.693 ± 0.037	1.32TyrAsn: 1.32 ± 0.047	1.61TyrPro: 1.61 ± 0.049	1.625TyrGln: 1.625 ± 0.047	2.137TyrArg: 2.137 ± 0.053	1.909TyrSer: 1.909 ± 0.059	2.108TyrThr: 2.108 ± 0.062	2.207TyrVal: 2.207 ± 0.06	0.358TyrTrp: 0.358 ± 0.025	1.332TyrTyr: 1.332 ± 0.048	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.003XaaAla: 0.003 ± 0.002	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.004XaaGly: 0.004 ± 0.003	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.01XaaPro: 0.01 ± 0.004	0.001XaaGln: 0.001 ± 0.002	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.04XaaXaa: 0.04 ± 0.01

Statistics based on 2410 proteins (675675 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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