Albugo candida
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13200 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A024GRH0|A0A024GRH0_9STRA Uncharacterized protein OS=Albugo candida OX=65357 GN=BN9_108320 PE=4 SV=1
MM1 pKa = 7.82 RR2 pKa = 11.84 LLASLQMISVALTVIGVTTTLQEE25 pKa = 4.3 VQSSSSSLRR34 pKa = 11.84 SLQSSDD40 pKa = 2.55 HH41 pKa = 6.03 SEE43 pKa = 3.94 RR44 pKa = 11.84 NNRR47 pKa = 11.84 PTISMVVISEE57 pKa = 4.09 YY58 pKa = 10.9 FPIPPTEE65 pKa = 4.27 EE66 pKa = 4.18 ADD68 pKa = 3.47 QSQNEE73 pKa = 4.35 PKK75 pKa = 10.64 VVIPDD80 pKa = 3.26 DD81 pKa = 3.99 TFNEE85 pKa = 4.55 KK86 pKa = 10.86 YY87 pKa = 10.77 GIFDD91 pKa = 3.77 GGDD94 pKa = 3.35 DD95 pKa = 3.78 EE96 pKa = 5.63 EE97 pKa = 4.88 EE98 pKa = 4.1 EE99 pKa = 4.43 EE100 pKa = 4.36 EE101 pKa = 4.47 EE102 pKa = 4.36 KK103 pKa = 11.22 VHH105 pKa = 6.33 TGYY108 pKa = 10.78 EE109 pKa = 4.26 LEE111 pKa = 4.47 CEE113 pKa = 4.2 GLEE116 pKa = 4.08 KK117 pKa = 10.9 DD118 pKa = 3.51 SAEE121 pKa = 4.19 SVNEE125 pKa = 3.62 ISSDD129 pKa = 3.82 YY130 pKa = 11.44 LLDD133 pKa = 4.14 CQDD136 pKa = 4.29 DD137 pKa = 4.12 EE138 pKa = 6.15 KK139 pKa = 11.82 VDD141 pKa = 4.58 LDD143 pKa = 4.09 TKK145 pKa = 10.87 KK146 pKa = 8.7 HH147 pKa = 4.97 TKK149 pKa = 7.31 EE150 pKa = 3.62 TGYY153 pKa = 10.83 GQTFFDD159 pKa = 5.58 CDD161 pKa = 3.91 DD162 pKa = 4.15 EE163 pKa = 6.76 GDD165 pKa = 3.92 NVDD168 pKa = 5.03 EE169 pKa = 4.31 VVQDD173 pKa = 3.58 KK174 pKa = 11.21 EE175 pKa = 4.38 EE176 pKa = 4.06 NTEE179 pKa = 3.73 EE180 pKa = 3.91 MRR182 pKa = 11.84 YY183 pKa = 9.41 EE184 pKa = 4.07 LQPLLDD190 pKa = 5.34 CDD192 pKa = 6.17 DD193 pKa = 4.15 EE194 pKa = 7.03 DD195 pKa = 4.61 EE196 pKa = 6.04 DD197 pKa = 5.3 INEE200 pKa = 3.84 VDD202 pKa = 3.51 QDD204 pKa = 3.73 KK205 pKa = 11.5 EE206 pKa = 4.3 EE207 pKa = 4.14 NTEE210 pKa = 3.79 ATRR213 pKa = 11.84 YY214 pKa = 9.21 NPQPILICDD223 pKa = 3.55 EE224 pKa = 5.57 DD225 pKa = 4.2 EE226 pKa = 6.64 DD227 pKa = 4.92 EE228 pKa = 6.7 DD229 pKa = 4.78 IDD231 pKa = 4.09 HH232 pKa = 7.24 KK233 pKa = 11.14 SNSMVLKK240 pKa = 10.59 EE241 pKa = 4.04 EE242 pKa = 4.17 AKK244 pKa = 10.55 SLQEE248 pKa = 4.46 SKK250 pKa = 10.88 RR251 pKa = 11.84 EE252 pKa = 4.06 EE253 pKa = 3.9 NWSEE257 pKa = 3.45 ILQYY261 pKa = 11.59 DD262 pKa = 4.03 SMQAYY267 pKa = 10.12 LDD269 pKa = 3.79 KK270 pKa = 10.79 EE271 pKa = 4.33 AEE273 pKa = 4.02 KK274 pKa = 10.9 SRR276 pKa = 11.84 EE277 pKa = 3.91 SEE279 pKa = 3.29 FDD281 pKa = 3.71 YY282 pKa = 11.55 NFGRR286 pKa = 11.84 SNDD289 pKa = 3.48 NEE291 pKa = 3.83 FRR293 pKa = 11.84 SCKK296 pKa = 9.93 SLCAYY301 pKa = 7.86 DD302 pKa = 3.83 TTGPACGSDD311 pKa = 2.86 GQTYY315 pKa = 10.86 SNEE318 pKa = 3.61 CMMRR322 pKa = 11.84 CLEE325 pKa = 4.27 PDD327 pKa = 2.52 IKK329 pKa = 10.78 YY330 pKa = 10.17 YY331 pKa = 10.83 HH332 pKa = 7.06 EE333 pKa = 5.57 GYY335 pKa = 10.43 CLQQEE340 pKa = 4.46 DD341 pKa = 4.47 SSCDD345 pKa = 3.2 VDD347 pKa = 4.87 CSHH350 pKa = 6.65 THH352 pKa = 7.18 DD353 pKa = 4.76 LVCGIDD359 pKa = 3.2 GQTYY363 pKa = 10.5 INYY366 pKa = 9.58 CLYY369 pKa = 11.02 AVTYY373 pKa = 9.84 CDD375 pKa = 4.6 KK376 pKa = 11.2 NLATLPFLFGACEE389 pKa = 4.06 SDD391 pKa = 3.56 KK392 pKa = 10.71 VTAFF396 pKa = 3.46
Molecular weight: 45.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.935
IPC_protein 3.935
Toseland 3.732
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.783
Rodwell 3.757
Grimsley 3.643
Solomon 3.897
Lehninger 3.846
Nozaki 3.999
DTASelect 4.19
Thurlkill 3.757
EMBOSS 3.795
Sillero 4.037
Patrickios 0.985
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.94
Protein with the highest isoelectric point:
>tr|A0A024GJV7|A0A024GJV7_9STRA Uncharacterized protein OS=Albugo candida OX=65357 GN=BN9_079480 PE=3 SV=1
MM1 pKa = 7.43 IGHH4 pKa = 7.22 RR5 pKa = 11.84 DD6 pKa = 3.47 SLRR9 pKa = 11.84 DD10 pKa = 3.31 AVIAKK15 pKa = 9.81 KK16 pKa = 10.84 VEE18 pKa = 4.21 TVGIRR23 pKa = 11.84 GDD25 pKa = 3.34 VCNTSRR31 pKa = 11.84 RR32 pKa = 11.84 EE33 pKa = 3.76 WRR35 pKa = 11.84 TVTNLKK41 pKa = 10.17 VSIMSKK47 pKa = 10.32 SFAHH51 pKa = 6.62 IDD53 pKa = 3.25 AEE55 pKa = 4.16 NRR57 pKa = 11.84 QGALIIACEE66 pKa = 4.12 PSTSFSGPYY75 pKa = 8.3 QVCRR79 pKa = 11.84 LSSTSEE85 pKa = 3.87 STLVRR90 pKa = 11.84 SVFLVVFKK98 pKa = 10.85 KK99 pKa = 10.81 AIGRR103 pKa = 11.84 CEE105 pKa = 3.87 EE106 pKa = 3.85 HH107 pKa = 6.43 CTRR110 pKa = 11.84 GVFVLRR116 pKa = 11.84 IRR118 pKa = 11.84 YY119 pKa = 8.38 HH120 pKa = 7.88 VGIEE124 pKa = 3.96 CCRR127 pKa = 11.84 NGVRR131 pKa = 11.84 TFVRR135 pKa = 11.84 LIGLRR140 pKa = 11.84 RR141 pKa = 11.84 RR142 pKa = 11.84 RR143 pKa = 3.54
Molecular weight: 16.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.399
IPC_protein 10.072
Toseland 10.584
ProMoST 10.54
Dawson 10.672
Bjellqvist 10.409
Wikipedia 10.877
Rodwell 10.804
Grimsley 10.701
Solomon 10.804
Lehninger 10.774
Nozaki 10.643
DTASelect 10.379
Thurlkill 10.584
EMBOSS 10.994
Sillero 10.628
Patrickios 10.555
IPC_peptide 10.818
IPC2_peptide 9.853
IPC2.peptide.svr19 8.669
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13200
0
13200
6569959
49
14607
497.7
56.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.788 ± 0.015
2.073 ± 0.01
5.405 ± 0.013
6.622 ± 0.021
4.079 ± 0.013
4.813 ± 0.017
2.847 ± 0.01
5.97 ± 0.015
5.802 ± 0.023
9.68 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.438 ± 0.007
4.321 ± 0.011
3.953 ± 0.015
4.651 ± 0.014
6.018 ± 0.017
8.817 ± 0.023
5.688 ± 0.013
5.968 ± 0.014
1.146 ± 0.006
2.923 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here